LOCUS       NM_019988               3361 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus MTOR associated protein, LST8 homolog (S. cerevisiae)
            (Mlst8), transcript variant 2, mRNA.
ACCESSION   NM_019988
VERSION     NM_019988.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   El-Saafin,F., Bergamasco,M.I., Chen,Y., May,R.E., Esakky,P.,
            Hediyeh-Zadeh,S., Dixon,M., Wilcox,S., Davis,M.J., Strasser,A.,
            Smyth,G.K., Thomas,T. and Voss,A.K.
  TITLE     Loss of TAF8 causes TFIID dysfunction and p53-mediated apoptotic
            neuronal cell death
  JOURNAL   Cell Death Differ 29 (5), 1013-1027 (2022)
   PUBMED   35361962
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Olthof,A.M., White,A.K., Mieruszynski,S., Doggett,K., Lee,M.F.,
            Chakroun,A., Abdel Aleem,A.K., Rousseau,J., Magnani,C.,
            Roifman,C.M., Campeau,P.M., Heath,J.K. and Kanadia,R.N.
  TITLE     Disruption of exon-bridging interactions between the minor and
            major spliceosomes results in alternative splicing around minor
            introns
  JOURNAL   Nucleic Acids Res 49 (6), 3524-3545 (2021)
   PUBMED   33660780
REFERENCE   3  (bases 1 to 3361)
  AUTHORS   Saxton,R.A. and Sabatini,D.M.
  TITLE     mTOR Signaling in Growth, Metabolism, and Disease
  JOURNAL   Cell 168 (6), 960-976 (2017)
   PUBMED   28283069
  REMARK    Review article
            Erratum:[Cell. 2017 Apr 6;169(2):361-371. doi:
            10.1016/j.cell.2017.03.035.. PMID: 28388417]
REFERENCE   4  (bases 1 to 3361)
  AUTHORS   Smithson,L.J. and Gutmann,D.H.
  TITLE     Proteomic analysis reveals GIT1 as a novel mTOR complex component
            critical for mediating astrocyte survival
  JOURNAL   Genes Dev 30 (12), 1383-1388 (2016)
   PUBMED   27340174
REFERENCE   5  (bases 1 to 3361)
  AUTHORS   Kakumoto,K., Ikeda,J., Okada,M., Morii,E. and Oneyama,C.
  TITLE     mLST8 Promotes mTOR-Mediated Tumor Progression
  JOURNAL   PLoS One 10 (4), e0119015 (2015)
   PUBMED   25906254
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3361)
  AUTHORS   Zhao,Q., Albsoul-Younes,A.M., Zhao,P., Kozasa,T., Nakajima,Y. and
            Nakajima,S.
  TITLE     Dominant negative effects of a Gbeta mutant on G-protein coupled
            inward rectifier K+ channel
  JOURNAL   FEBS Lett 580 (16), 3879-3882 (2006)
   PUBMED   16797547
  REMARK    GeneRIF: The mutant, Gbeta1(W332A) protein, although inactive,
            retains its ability to bind Kir3 and prevents the wild type Gbeta
            from activating the channel.
REFERENCE   7  (bases 1 to 3361)
  AUTHORS   Jacinto,E., Loewith,R., Schmidt,A., Lin,S., Ruegg,M.A., Hall,A. and
            Hall,M.N.
  TITLE     Mammalian TOR complex 2 controls the actin cytoskeleton and is
            rapamycin insensitive
  JOURNAL   Nat Cell Biol 6 (11), 1122-1128 (2004)
   PUBMED   15467718
REFERENCE   8  (bases 1 to 3361)
  AUTHORS   Sarbassov,D.D., Ali,S.M., Kim,D.H., Guertin,D.A., Latek,R.R.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     Rictor, a novel binding partner of mTOR, defines a
            rapamycin-insensitive and raptor-independent pathway that regulates
            the cytoskeleton
  JOURNAL   Curr Biol 14 (14), 1296-1302 (2004)
   PUBMED   15268862
REFERENCE   9  (bases 1 to 3361)
  AUTHORS   Kim,D.H., Sarbassov,D.D., Ali,S.M., Latek,R.R., Guntur,K.V.,
            Erdjument-Bromage,H., Tempst,P. and Sabatini,D.M.
  TITLE     GbetaL, a positive regulator of the rapamycin-sensitive pathway
            required for the nutrient-sensitive interaction between raptor and
            mTOR
  JOURNAL   Mol Cell 11 (4), 895-904 (2003)
   PUBMED   12718876
REFERENCE   10 (bases 1 to 3361)
  AUTHORS   Rodgers,B.D., Levine,M.A., Bernier,M. and Montrose-Rafizadeh,C.
  TITLE     Insulin regulation of a novel WD-40 repeat protein in adipocytes
  JOURNAL   J Endocrinol 168 (2), 325-332 (2001)
   PUBMED   11182770
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK040100.1 and AC154237.1.
            
            On Nov 16, 2011 this sequence version replaced NM_019988.4.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the
            longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK040100.1, BC015279.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2463              AK040100.1         2-2464
            2464-3361           AC154237.1         133629-134526       c
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 12.39 cM"
     gene            1..3361
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /note="MTOR associated protein, LST8 homolog (S.
                     cerevisiae)"
                     /db_xref="GeneID:56716"
                     /db_xref="MGI:MGI:1929514"
     exon            1..67
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            68..226
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     CDS             98..1078
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /note="isoform 1 is encoded by transcript variant 2;
                     target of rapamycin complex subunit LST8; protein GbetaL;
                     TORC subunit LST8; mammalian lethal with SEC13 protein 8;
                     transducin (beta)-like 4"
                     /codon_start=1
                     /product="target of rapamycin complex subunit LST8 isoform
                     1"
                     /protein_id="NP_064372.2"
                     /db_xref="CCDS:CCDS28484.1"
                     /db_xref="GeneID:56716"
                     /db_xref="MGI:MGI:1929514"
                     /translation="MNTTPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQ
                     VNALEITPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVSKNIASVGFHEDGRWMYT
                     GGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTD
                     HNEQLIPEPESSITSAHIDPDASYMAAVNSAGNCYVWNLTGGIGDDVTQLIPKTKIPA
                     HTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSSNPGESSRGWMWGC
                     AFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG"
     exon            227..278
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            279..441
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            442..517
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            518..670
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            671..795
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            796..959
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     exon            960..3361
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3339..3344
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /note="hexamer: AATAAA"
     polyA_site      3360
                     /gene="Mlst8"
                     /gene_synonym="0610033N12Rik; Gbl"
                     /note="major polyA site"
ORIGIN      
        1 ttctatcgac tagctcctcc tcgcctcgcc gtcctggact aaggcagagt gcagagcgtg
       61 tgttaaggtc tcattttttt tctccagcca tcaaacaatg aataccaccc caggcacagt
      121 gggcagtgac cctgtcatct tagcaactgc aggctatgac cacacggtgc gcttttggca
      181 ggctcacagt ggaatctgca ctcgaacagt gcagcatcag gactctcagg taaatgcatt
      241 ggagattact ccggaccgaa gcatgattgc tgctgcaggt taccaacata tccgcatgta
      301 tgatctcaac tccaataacc ccaaccccat catcagttat gacggagtca gtaagaacat
      361 tgcatcagtg ggctttcacg aggatggtcg ctggatgtat acaggtggcg aggactgcac
      421 agctcgcatc tgggacctca ggtcccggaa cttgcagtgt cagcgtatct tccaggtgaa
      481 cgcacccatt aattgcgtgt gtctgcatcc caaccaggca gaactcattg tgggtgatca
      541 gagcggtgct atccacatct gggacctgaa gacagaccac aatgagcagc tgattcccga
      601 gcctgagtct tccatcacgt ctgctcacat cgacccagat gctagctaca tggcagccgt
      661 caatagtgcc ggaaactgct atgtctggaa cctgacaggg ggcattggtg acgatgtgac
      721 tcagctcatc cctaagacca agatcccagc ccatacacgc tatgccctgc aatgccgctt
      781 cagccctgat tccacgcttc ttgccacctg ttcagctgac cagacatgta aaatctggag
      841 gacatccaac ttctccctga tgacagagct tagcatcaag agtagtaacc ctggagagtc
      901 atcccgtggc tggatgtggg gctgtgcctt ctcaggggat tcccagtaca ttgtcacagc
      961 ttcttctgac aacctagccc ggctctggtg tgtagagact ggagaaatca agagagagta
     1021 tggtggccat cagaaagctg tcgtctgctt ggccttcaat gacagtgtgc tgggttagac
     1081 ttggcccttg aggctgcatt gggcaatcag gactgcctgg tatagtgacg ccaagaggat
     1141 gtatctgaac aatacccggg tccaagtgag actctcaggt tagcatgtca ggccagttgg
     1201 acctgaccgg actatgcagg cccctcctgg ccaacctaga ctttttggct actctacgct
     1261 gggactcttt ccatgcccag tttgttgtca gaaagctcac tcactaagcc agactggact
     1321 gggagtcttt gctcctttag agagactaga cctagaggac ttgggactgt tgaggaggtc
     1381 agaggttaat gcctgtattc caggtatgtt accccgactt gcttcagaaa ttaccattat
     1441 ggccaggcaa tctgggttta ttaatcccta ctgtcagctg ccttccatag gtactggtga
     1501 cctggccagc ttactgacct gtggggacag gactggccta tggaaccaaa ggccaggagg
     1561 gcttgggtag ggggtcttag tctgggaggt aataaaagca gacagacaca cagatgttgc
     1621 tcgggaagca gatgtcaatg cagaggtaga tcagccgctg tctctggggc cctggggaga
     1681 agggaacttc gtgaatccca agggccccct acccagtact gtcctacagg agccatagtc
     1741 ctcttcaccc aacctatccc aatttacctt ctgcccattg ggtccagtag atgggagtgt
     1801 ctgcacaaag gtgttgcact gaaactccca cttgggtagg gtccacagta tggcccccca
     1861 aaactgccag cagctcctgg gcatagtggg tatcccggcc aggtacatgt gactcttggg
     1921 cctgcagaaa gactctggtg gaccaccgta tagtacttcc ttgctcctgg ctgggaaggg
     1981 tattggtgcc caccccaggc tgatggcccc tccacctgcc cttgtcaaca gccttagtca
     2041 ggggaaaata gagctttctg ttcccctgaa accagagcct tggctggccc agctggactg
     2101 cacagaggac cttttgttta tcccctggcc ttgggggttt gtgggtggca agccaagtag
     2161 tactgctcac cctggaagtg ttcagcagca gctgcagggt ctcccaatct tgggcttcca
     2221 tcagacggag gaagcaggct tggtgctctg cagggcggta acagatgtag ccctgggggt
     2281 ggcggggcag ataggtaagt cgaggccacc cctgacacct ccactctctc tctctcccaa
     2341 gcccactcac gttctgcccg tcaaacagca cagcccagct gtggttgctc tgaaacggag
     2401 tcaccacgat ggttgccatg ttctgggcca catccactag agtagtttgg ttgggccagg
     2461 gtaccctgga gctcgggaag gtctttcgga gcatctgcag caacggctaa gggatatttg
     2521 ggattcagcc ttaacctatg tttccacatc cttctggttc tctatctgct cgaggctggt
     2581 atctagacct ggccccatgc gagaactgac aggtactggc cttcctgccc tcttcccact
     2641 ctgcataccc cgaggcttga agaggtgctt accttgggag agctgtacat gaagccaaga
     2701 agccccccag ccacggcccc cgcagtgagc agtgtcagca gcagcagcag gcctgctgct
     2761 ctccagcccc tatggcaggg tttggtcttc acctgagggc acagtactgg ccgtggggct
     2821 tctggcttag agactcaggg aaggggctga ggatggtatg tggtaacatt cctttcttct
     2881 atggcatccc tgagttcttg tcttgggggt ggtagagact tacctcagca ggcttggacc
     2941 tgaggctctc agtgtggatg cgcccttgct tcatgctgca gcccctgctg acaatgcact
     3001 gattgtctgc agccccttgg aggagtcagc cagggaaacc tcaggatgcc tttgatgaat
     3061 caaactgtat cctccccttc caaccccact gcttccttcc cagccagtat ctgatatcct
     3121 gcctcagcca ggacaaccct ctcccccaca tgtgattgct ggtcactagt ttgtctctgg
     3181 actcttggcc tgtgcagaac atggggtctt ggtgtgtgtt agagctggcc ttgaagtcag
     3241 aattggctag gtcttcgcca gggtgatttt gatctggttg ggcagacttt ttttcttggt
     3301 gttgcctccg gactcctgag ctagacaccg gaactatcaa taaatgtgta tgaaccatca
     3361 g
//
