LOCUS       NM_053015               4484 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus melanophilin (Mlph), mRNA.
ACCESSION   NM_053015
VERSION     NM_053015.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Zhao,K., Matsunaga,K., Mizuno,K., Wang,H., Okunishi,K. and Izumi,T.
  TITLE     Functional hierarchy among different Rab27 effectors involved in
            secretory granule exocytosis
  JOURNAL   Elife 12, e82821 (2023)
   PUBMED   36803984
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4484)
  AUTHORS   Myung,C.H., Lee,J.E., Jo,C.S., Park,J.I. and Hwang,J.S.
  TITLE     Regulation of Melanophilin (Mlph) gene expression by the
            glucocorticoid receptor (GR)
  JOURNAL   Sci Rep 11 (1), 16813 (2021)
   PUBMED   34413386
  REMARK    GeneRIF: Regulation of Melanophilin (Mlph) gene expression by the
            glucocorticoid receptor (GR).
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4484)
  AUTHORS   Wang,H., Mizuno,K., Takahashi,N., Kobayashi,E., Shirakawa,J.,
            Terauchi,Y., Kasai,H., Okunishi,K. and Izumi,T.
  TITLE     Melanophilin Accelerates Insulin Granule Fusion without Predocking
            to the Plasma Membrane
  JOURNAL   Diabetes 69 (12), 2655-2666 (2020)
   PUBMED   32994278
REFERENCE   5  (bases 1 to 4484)
  AUTHORS   Cao,Q.J., Zhang,N., Zhou,R., Yao,L.L. and Li,X.D.
  TITLE     The cargo adaptor proteins RILPL2 and melanophilin co-regulate
            myosin-5a motor activity
  JOURNAL   J Biol Chem 294 (29), 11333-11341 (2019)
   PUBMED   31175157
  REMARK    GeneRIF: propose that Rab36 stimulates RILPL2 to interact with the
            myosin-5a GTD; this interaction then induces exposure of the
            melanophilin-binding site in the GTD, enabling melanophilin to
            interact with the GTD and activate myosin-5a motor activity
REFERENCE   6  (bases 1 to 4484)
  AUTHORS   Wilcox,F.H., Hirschhorn,L., Taylor,B.A., Womack,J.E. and
            Roderick,T.H.
  TITLE     Genetic variation in alkaline phosphatase of the house mouse (Mus
            musculus) with emphasis on a manganese-requiring isozyme
  JOURNAL   Biochem Genet 17 (11-12), 1093-1107 (1979)
   PUBMED   540002
REFERENCE   7  (bases 1 to 4484)
  AUTHORS   Plant,J. and Glynn,A.A.
  TITLE     Locating salmonella resistance gene on mouse chromosome 1
  JOURNAL   Clin Exp Immunol 37 (1), 1-6 (1979)
   PUBMED   385184
REFERENCE   8  (bases 1 to 4484)
  AUTHORS   Bradley,D.J., Taylor,B.A., Blackwell,J., Evans,E.P. and Freeman,J.
  TITLE     Regulation of Leishmania populations within the host. III. Mapping
            of the locus controlling susceptibility to visceral leishmaniasis
            in the mouse
  JOURNAL   Clin Exp Immunol 37 (1), 7-14 (1979)
   PUBMED   290436
REFERENCE   9  (bases 1 to 4484)
  AUTHORS   LaVail,J.H., Nixon,R.A. and Sidman,R.L.
  TITLE     Genetic control of retinal ganglion cell projections
  JOURNAL   J Comp Neurol 182 (3), 399-421 (1978)
   PUBMED   102659
REFERENCE   10 (bases 1 to 4484)
  AUTHORS   Wilson,C.M., Erdos,E.G., Wilson,J.D. and Taylor,B.A.
  TITLE     Location on chromosome 1 of Rnr, a gene that regulates renin in the
            submaxillary gland of the mouse
  JOURNAL   Proc Natl Acad Sci U S A 75 (11), 5623-5626 (1978)
   PUBMED   281712
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118682.8, AK019883.1 and AK148274.1.
            
            [WARNING] On Jul 25, 2024 this sequence was replaced by
            NM_053015.4.
            
            On Jun 19, 2009 this sequence version replaced NM_053015.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150804.1, AK148274.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC118682.8         106628-106656       c
            30-697              AK019883.1         2-669
            698-752             AK148274.1         670-724
            753-1533            AK019883.1         724-1504
            1534-1600           AK148274.1         1506-1572
            1601-2160           AK019883.1         1572-2131
            2161-4484           AC118682.8         70614-72937         c
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 45.73 cM"
     gene            1..4484
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /note="melanophilin"
                     /db_xref="GeneID:171531"
                     /db_xref="MGI:MGI:2176380"
     exon            1..154
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            155..288
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1951
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /note="slaC2-a; exophilin-3; leaden protein;
                     synaptotagmin-like protein 2a; slp homolog lacking C2
                     domains a"
                     /codon_start=1
                     /product="melanophilin"
                     /protein_id="NP_443748.2"
                     /db_xref="CCDS:CCDS15155.1"
                     /db_xref="GeneID:171531"
                     /db_xref="MGI:MGI:2176380"
                     /translation="MGKRLDLSTLTDEEAEHVWAVVQRDFDLRRREEERLQGLKGKIQ
                     KESSKRELLSDTAHLNETHCARCLQPYRLLLNSRRQCLECSLFVCKSCSHAHPEEQGW
                     LCDPCHLARVVKIGSLEWYYQHVRARFKRFGSAKVIRSLCGRLQGGGGSEPSLEEGNG
                     DSEQTDEDGDLDTEARDQPLNSKKKKRLLSFRDVDFEEDSDHLVQPCSQTLGLSSVPE
                     SAHSLQSLSGEPYSEDTTSLEPEGLEETGARALGCHPSPEVQPCSPLPSGEDAHAELD
                     SPAASCKSAFGTTAMPGTDDVRGKHLPSQYLADVDTSDEDSIQGPRAASQHSKRRART
                     VPETQILELNKRMSAVEHLLVHLENTVLPPSAQEPTVETHPSADTEEETLRRRLEELT
                     SNISGSSTSSEDETKPDGTFLGGSPKVCTDTGHMETQERNPRSPGNPARPTKSTDEEL
                     SEMEDRVAMTASEVQQAESEISDIQSRIAALRAAGLTVKPSGKPRRKSGIPIFLPRVT
                     EKLDRIPKTPPADPDDQAKMPKATTAVPSLLRRKYSPSSQGVDSGSFDRKSVYRGSLT
                     QRNPNGRRGTARHIFAKPVMAQQP"
     exon            289..510
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            511..623
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            624..727
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            728..850
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1055
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1195
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1279
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1432
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1588
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1681
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1682..1768
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1823
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1924
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1925..4484
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2249
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
     regulatory      4456..4461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /note="hexamer: AATAAA"
     polyA_site      4484
                     /gene="Mlph"
                     /gene_synonym="2210418F23Rik; 5031433I09Rik; D1Wsu84e;
                     l(1)-3Rk; l1Rk3; ln; Slac-2a"
                     /note="major polyA site"
ORIGIN      
        1 ggatccccgc acacagctct gtctagctca gtgcaccctg acactggcct cgctcggtgc
       61 ctggggtttc tctctcagga gctaagagaa aggagcagag gccaggaccc accgagccag
      121 agctacaggc tgggcattcc caggagagag gctggtgtgg cctggatgag gcgccgagat
      181 ggggaaaagg ttggaccttt ccacgctcac agacgaggag gctgagcacg tgtgggcagt
      241 ggttcagcgg gactttgacc tcaggaggcg agaggaagaa agactccagg ggctgaaggg
      301 caaaatacaa aaggagagct ccaagaggga gctgctgtcg gacacagccc atctgaatga
      361 gactcactgt gcccgctgcc tgcagcccta ccggctgctc ctgaacagca gacgacagtg
      421 cctagagtgc agcctcttcg tctgcaaaag ctgcagccac gcccacccag aagagcaggg
      481 ctggctctgc gacccctgcc acctggccag ggtcgtgaag atcggttctc tggagtggta
      541 ctaccagcac gtgagggctc gcttcaagcg tttcgggagt gccaaagtga tccggtctct
      601 ctgtgggcgg ctgcagggtg gaggtggatc tgagccaagc ttggaagaag gaaatggaga
      661 cagtgagcag actgatgagg atggagacct ggacacagag gccagagacc agcccctcaa
      721 cagcaaaaag aaaaagcgcc tgctctcctt ccgagatgtg gactttgagg aagactcaga
      781 ccacttggtg cagccttgca gccaaacctt gggcctgtcc tcagtccctg agtctgcaca
      841 cagcctgcag tccctctcag gtgagcccta ctctgaggac accacctctc tggagcccga
      901 gggcctagag gagactggtg caagggcttt gggatgtcat cccagtcctg aagtgcagcc
      961 atgtagccct ttaccctctg gggaggatgc tcacgctgaa ctggactcgc ctgcagcatc
     1021 ctgcaagagt gcctttggga ccacagctat gcctggaaca gacgatgtca ggggcaaaca
     1081 tctgccctca cagtacctgg ctgatgtaga cacctctgat gaagacagta tccagggtcc
     1141 cagggcagcc tcccagcata gcaagaggag ggcccggact gtgcctgaga ctcagatctt
     1201 ggagttgaac aagcgaatgt cagctgtgga gcacctgcta gtccacctgg agaatacggt
     1261 tctgccaccc tcagcccagg aaccaactgt ggagacacac cccagtgctg acacagagga
     1321 ggagacactc aggaggaggc tggaggagct gaccagcaac atcagtggtt ccagcacctc
     1381 atcagaagat gagaccaagc cagatggcac cttccttgga gggtccccaa aggtgtgcac
     1441 agacacaggg cacatggaga cacaggaaag gaaccctcgg agccctggga accctgctcg
     1501 gcctacaaaa agcacagatg aggagctctc tgagatggag gatagagtgg ccatgacagc
     1561 ctctgaagtt cagcaggctg agagtgagat ctcagacatc cagtccagga tcgcagctct
     1621 gagagccgca ggactcacag tgaagccctc gggaaaacct cggagaaagt caggcatccc
     1681 gatctttctt ccccgcgtta ctgagaaact tgacaggatc ccaaagactc cacctgcaga
     1741 ccctgatgac caagccaaga tgcccaaggc aacaacagct gtgccctctc tcctgaggag
     1801 gaagtattct cccagcagcc aaggcgtaga cagtggttct tttgatcgga aatcagtgta
     1861 ccgtggctcg ctgacacaaa ggaaccctaa cgggaggaga gggacagcca gacacatctt
     1921 cgcgaaaccc gtgatggccc agcagcccta aagggaggct catggcacag gacagtacca
     1981 caaagcaccc cacagtttcc tccacatgac cgtcgcctcc ccatgggctc tgttctttgt
     2041 gatgctcaca gctgcttgta gtcatcattc cagtgaggag taagaagaat tttatgtgtg
     2101 tctctacctg tgagactgca gaatctcaat cctccagctg tcaccaggtc accaaccagg
     2161 cacctgccag cctctgacct gggaaataca aactctgtga cttctcctca actgatgttc
     2221 tctgcctact gaaggtcact cagaagccat ctcgctgtga ttctttaacc cgatcctcga
     2281 ctttgctggg ccaccctcac agctgggatg aaaagagctg gttcagagga agccctgaac
     2341 atgtacacat ccatgactat gcacgagcat gagagagaga gagagagaga gagagagaga
     2401 gagagagaga gagagaggaa gagagagaga gagagaatga atctgtgggc atgatgagag
     2461 agcatacaat tgcaggtggt gaatggggga ggggtggatt cctcccctac ctgacagatt
     2521 ttgttcttca aacttccttg tgtgttctag atgagggcag ataacactga ccacccccac
     2581 cccagcattc ccaccacaat aaccactatc cacaatgcca cactaagacc aagcctccaa
     2641 cacaggcttt tggggtacac tacatgggga cctcgctttc tagatagctt gtcttcaaat
     2701 gcaggcttct aacacacaga gatgactggg atttatcaag aactcagatc ttccaggtca
     2761 agcccgtcag agccagtttt gctggtgtct gcacggtctg cccttcacag ccatcccacc
     2821 actacagcgg ggctgctacc cacacccagc gctgcctctt tcctgtcgtc ggtttgtatc
     2881 ctgttctggg ctagagaaca cacaggccag agccacaaac atccccgtgt cttctgaaaa
     2941 taaactccag gaggaagcct ctcactcatt cagagctcct ctcaggagcg ccatatttgt
     3001 tacaacctga tgcaggaaat gtgtcctcct gtgtcacctg tgccctgaac tgcttgccac
     3061 ctgaccctac ctgcccttgg gggttggagg tgaggtggcg cagagtcaac aacacagact
     3121 ccgggaggtg gtgggaaaca gaaacgctgc agcaagttca tctgaacaat gctctttaat
     3181 accctccacc caagccccat tggtcccagg aaagcacctc ctcctcctcc tcttcttcct
     3241 cctcctcctc ctcctcctcc tcttcctcct cctcttcctc ctcctcctcc tcttcctcct
     3301 cctcttcctc ctcctcttcc tcctcctcct cttcctcctc ctcctcttcc tcctcctctt
     3361 cctcctcctc ctcctcctcc tcctcctctt cctcctcctc ttcctcctcc tcttcctcct
     3421 cctcttcctc ctcctcctcc tcctcctctt cctcctcctc ctcctcctcc tcctcctctt
     3481 cctcctcctc ttcctcctcc tcttcctcct cctcctcctc ttcctcctcc tcctcctcct
     3541 cctcttcctc ctcctcctcc tcttcctcct cctcctcctc cttctcctcc tcctcctcct
     3601 cctcctcctc ctcctcttcc tcctcctcca ccttgttcta gagttcctta ccatacccac
     3661 ctcccaagga gcccactggc cagagccctc ttccaaggaa gaggcagcca ttccttgaga
     3721 acccagatgc tacgctgacc acgtgtccaa aggatgacgg ggcagcctaa agcttttcta
     3781 caagtctggt ggtgggcagg aaatggtgat cctggggaga cacctgcctg gtctcttctc
     3841 gtgcacagct ccttcaagaa tgttctattg cacctcaatg atatttcata taacctgtac
     3901 ctattgtcgt ggtgatgacg gggtgagtta aatggcagaa cgttgaacag tcctttcacc
     3961 agcacagacc tgcagatact tcaacacttc cagaatatat gctggtgaaa gaatgaggct
     4021 cagcacctct aagcgggctc aggagtcttc ccagaaggca caccccgaaa ggcaaacact
     4081 gacctttcca ggtgatctga gctcttctat ctatttttgt gtgcactaat tctttctcat
     4141 tacatgtaaa ttacctttcc agcataatag acttcctttt ttaaaagatt tttttgcctg
     4201 cgtgtatggg gtgaggtgtg tacacatcta tgaggggacg ctgtgcactt gtgcatagag
     4261 accagagatt aatgtggtgt cttcctcagt catgtctcca tttcctgttt ttgagacact
     4321 gatcttggag gttgtcaatt tggctaagct gactggccag tgagcatcag gggtcttcca
     4381 cacctctgct ctctctacct tagcactggg gttataaacc ggcgctgcca tgttctgcct
     4441 ttaaacctac cttacaataa aagtaacact ctgtttcgtt ttca
//
