LOCUS       NM_029005               2007 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus mixed lineage kinase domain-like (Mlkl), transcript
            variant 2, mRNA.
ACCESSION   NM_029005 XM_001003995 XM_001003998 XM_356104 XM_895027 XM_916960
            XM_924589
VERSION     NM_029005.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Jena,K.K., Mambu,J., Boehmer,D., Sposito,B., Millet,V., de Sousa
            Casal,J., Muendlein,H.I., Spreafico,R., Fenouil,R., Spinelli,L.,
            Wurbel,S., Riquier,C., Galland,F., Naquet,P., Chasson,L.,
            Elkins,M., Mitsialis,V., Ketelut-Carneiro,N., Bugda Gwilt,K.,
            Thiagarajah,J.R., Ruan,H.B., Lin,Z., Lien,E., Shao,F., Chou,J.,
            Poltorak,A., Ordovas-Montanes,J., Fitzgerald,K.A., Snapper,S.B.,
            Broggi,A. and Zanoni,I.
  TITLE     Type III interferons induce pyroptosis in gut epithelial cells and
            impair mucosal repair
  JOURNAL   Cell 187 (26), 7533-7550 (2024)
   PUBMED   39500322
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Hanggi,K., Li,J., Gangadharan,A., Liu,X., Celias,D.P.,
            Osunmakinde,O., Keske,A., Davis,J., Ahmad,F., Giron,A.,
            Anadon,C.M., Gardner,A., DeNardo,D.G., Shaw,T.I., Beg,A.A., Yu,X.
            and Ruffell,B.
  TITLE     Interleukin-1alpha release during necrotic-like cell death
            generates myeloid-driven immunosuppression that restricts
            anti-tumor immunity
  JOURNAL   Cancer Cell 42 (12), 2015-2031 (2024)
   PUBMED   39577420
REFERENCE   3  (bases 1 to 2007)
  AUTHORS   Liu,X., Zhang,J., Zhang,D., Pan,Y., Zeng,R., Xu,C., Shi,S., Xu,J.,
            Qi,Q., Dong,X., Wang,J., Liu,T. and Dong,L.
  TITLE     Necroptosis plays a role in TL1A-induced airway inflammation and
            barrier damage in asthma
  JOURNAL   Respir Res 25 (1), 271 (2024)
   PUBMED   38987753
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2007)
  AUTHORS   Mannion,J., Gifford,V., Bellenie,B., Fernando,W., Ramos Garcia,L.,
            Wilson,R., John,S.W., Udainiya,S., Patin,E.C., Tiu,C., Smith,A.,
            Goicoechea,M., Craxton,A., Moraes de Vasconcelos,N., Guppy,N.,
            Cheung,K.J., Cundy,N.J., Pierrat,O., Brennan,A., Roumeliotis,T.I.,
            Benstead-Hume,G., Alexander,J., Muirhead,G., Layzell,S., Lyu,W.,
            Roulstone,V., Allen,M., Baldock,H., Legrand,A., Gabel,F.,
            Serrano-Aparicio,N., Starling,C., Guo,H., Upton,J., Gyrd-Hansen,M.,
            MacFarlane,M., Seddon,B., Raynaud,F., Roxanis,I., Harrington,K.,
            Haider,S., Choudhary,J.S., Hoelder,S., Tenev,T. and Meier,P.
  TITLE     A RIPK1-specific PROTAC degrader achieves potent antitumor activity
            by enhancing immunogenic cell death
  JOURNAL   Immunity 57 (7), 1514-1532 (2024)
   PUBMED   38788712
REFERENCE   5  (bases 1 to 2007)
  AUTHORS   Rodriguez,D.A., Tummers,B., Shaw,J.J.P., Quarato,G., Weinlich,R.,
            Cripps,J., Fitzgerald,P., Janke,L.J., Pelletier,S., Crawford,J.C.
            and Green,D.R.
  TITLE     The interaction between RIPK1 and FADD controls perinatal lethality
            and inflammation
  JOURNAL   Cell Rep 43 (6), 114335 (2024)
   PUBMED   38850531
REFERENCE   6  (bases 1 to 2007)
  AUTHORS   Wu,J., Huang,Z., Ren,J., Zhang,Z., He,P., Li,Y., Ma,J., Chen,W.,
            Zhang,Y., Zhou,X., Yang,Z., Wu,S.Q., Chen,L. and Han,J.
  TITLE     Mlkl knockout mice demonstrate the indispensable role of Mlkl in
            necroptosis
  JOURNAL   Cell Res 23 (8), 994-1006 (2013)
   PUBMED   23835476
  REMARK    GeneRIF: Neither Mlkl nor Rip3 deficiency provided protection
            against polymicrobial sepsis-induced animal death in the study of
            septic shock induced by CLP.
REFERENCE   7  (bases 1 to 2007)
  AUTHORS   Chen,W., Zhou,Z., Li,L., Zhong,C.Q., Zheng,X., Wu,X., Zhang,Y.,
            Ma,H., Huang,D., Li,W., Xia,Z. and Han,J.
  TITLE     Diverse sequence determinants control human and mouse receptor
            interacting protein 3 (RIP3) and mixed lineage kinase domain-like
            (MLKL) interaction in necroptotic signaling
  JOURNAL   J Biol Chem 288 (23), 16247-16261 (2013)
   PUBMED   23612963
  REMARK    GeneRIF: the importance of the RIP3-MLKL interaction in the
            formation of functional necrosomes and suggest that translocation
            of necrosomes to mitochondria-associated membranes is essential for
            necroptosis signaling.
REFERENCE   8  (bases 1 to 2007)
  AUTHORS   Zhao,J., Jitkaew,S., Cai,Z., Choksi,S., Li,Q., Luo,J. and Liu,Z.G.
  TITLE     Mixed lineage kinase domain-like is a key receptor interacting
            protein 3 downstream component of TNF-induced necrosis
  JOURNAL   Proc Natl Acad Sci U S A 109 (14), 5322-5327 (2012)
   PUBMED   22421439
REFERENCE   9  (bases 1 to 2007)
  AUTHORS   Sun,L., Wang,H., Wang,Z., He,S., Chen,S., Liao,D., Wang,L., Yan,J.,
            Liu,W., Lei,X. and Wang,X.
  TITLE     Mixed lineage kinase domain-like protein mediates necrosis
            signaling downstream of RIP3 kinase
  JOURNAL   Cell 148 (1-2), 213-227 (2012)
   PUBMED   22265413
  REMARK    GeneRIF: Findings implicate MLKL as a key mediator of necrosis
            signaling downstream of the kinase RIP3.
REFERENCE   10 (bases 1 to 2007)
  AUTHORS   Bisson,N., Tremblay,M., Robinson,F., Kaplan,D.R., Trusko,S.P. and
            Moss,T.
  TITLE     Mice lacking both mixed-lineage kinase genes Mlk1 and Mlk2 retain a
            wild type phenotype
  JOURNAL   Cell Cycle 7 (7), 909-916 (2008)
   PUBMED   18414056
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC163625.5, BY740989.1,
            AK018636.1 and BY467591.1.
            
            On Jul 16, 2015 this sequence version replaced NM_029005.2.
            
            Summary: This gene belongs to the protein kinase superfamily. The
            encoded protein contains a protein kinase-like domain; however, is
            thought to lack protein kinase activity. This protein plays a
            critical role in tumor necrosis factor (TNF)-induced necroptosis, a
            programmed cell death process, via interaction with
            receptor-interacting protein 3 (Rip3), which is a key signaling
            molecule in necroptosis pathway. Knockout of this gene in mice
            showed that it is essential for necroptosis. Alternatively spliced
            transcript variants have been found for this gene. [provided by
            RefSeq, Sep 2015].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region compared to variant 1. It
            encodes isoform 2, which is shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023755.1, AK170260.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AC163625.5         227457-227462       c
            7-39                BY740989.1         1-33
            40-1721             AK018636.1         14-1695
            1722-1820           BY467591.1         193-291
            1821-1867           AK018636.1         1819-1865
            1868-1868           BY467591.1         338-338
            1869-2004           AK018636.1         1867-2002
            2005-2007           AC163625.5         201054-201056       c
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.98 cM"
     gene            1..2007
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /note="mixed lineage kinase domain-like"
                     /db_xref="GeneID:74568"
                     /db_xref="MGI:MGI:1921818"
     exon            1..382
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            383..815
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            816..889
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1073
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1171
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1307
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1383
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1535
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1585
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1726
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..2007
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1986..1991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2007
                     /gene="Mlkl"
                     /gene_synonym="9130019I15Rik"
                     /note="major polyA site"
ORIGIN      
        1 cggagcagaa ctctggacag aaatagctgt cggacaggtt ggttttgagg actctgctgg
       61 agcattgctg cgttggccca aatttgactt taggcgggaa ccgcggctgg tgcggagttc
      121 ctccgagagg cgagagcttt aaggttgcac caagggaggg gactcaggac accggggacc
      181 tgctccgacc tggcgtgcat tgtcaacgag gcagaagacc gcggtccggg agcggcggga
      241 gactgatgat cctacaggaa ctgagcgcag gatagaccgg acgagcgccc ctcggagatc
      301 acgtagaatc cagtgagctg tagcgcggca ggaggaagca ggagaatccc agaatcacag
      361 attggacttc ctggaactta ggctatggat aaattgggac agatcatcaa gttaggccag
      421 ctcatctatg aacagtgtga aaagatgaaa tactgccgga aacaatgcca gcgtctagga
      481 aaccgtgtgc acggcctgct acagcctctc cagagactcc aggcccaagg aaagaagaac
      541 ctgcccgatg acattactgc tgccctgggc cgttttgatg aagtcctgaa ggaggctaac
      601 cagcagatag aaaagttcag caagaagtcc catatttgga agtttgtgag tgtgggcaat
      661 gataagatcc tcttccatga agtgaatgag aagctgagag acgtctggga ggagctgttg
      721 ctgctgcttc aggtttatca ttggaatacc gtttcagatg tcagccagcc agcatcctgg
      781 cagcaggaag atcgacagga tgcagaggaa gacggaaatg aaaatatgaa agttatcctg
      841 atgcagttgc aaattagcgt ggaagaaatc aacaaaaccc tgaagcaatg ctcactaaaa
      901 cccacacagg agatcccaca agatctccaa atcaaggaga ttccaaagga acatcttgga
      961 cctccgtgga ccaaactgaa gacaagtaaa atgagcacca tttatagagg agagtatcac
     1021 agatctccag ttaccatcaa agtattcaac aacccccagg ccgaaagtgt tggaatagtg
     1081 aggttcactt tcaatgacga gatcaaaacc atgaagaaat tcgattctcc caacatcttg
     1141 cgtatatttg ggatttgcat tgatcaaaca gtgaagcccc ctgagttctc cattgtcatg
     1201 gagtactgtg aacttggaac cctgagggaa ctgctggata gagaaaaaga cctcacaatg
     1261 agtgtgcgca gcctcctagt cctgagggca gccagaggct tatacaggct acaccattcg
     1321 gaaacactcc acagaaacat cagcagctcc agtttcctcg tagccggagg ctaccaagta
     1381 aagcttgcag gatttgagtt aagcaaaaca cagaattcca tcagccggac agcaaagagc
     1441 actaaagcag agagatccag ttcaacgata tatgtctccc ctgagagact gaaaaatcca
     1501 ttttgccttt atgacataaa agctgaaata tatagctttg gaattgtact ctgggaaatt
     1561 gccactggaa agatcccatt tgaaggctgt gattctaaga agatccggga gctggtggct
     1621 gaggacaaga agcaggaacc agtgggtcag gattgccctg agttgttgcg ggaaatcatt
     1681 aatgagtgtc gtgcccatga gccctcccaa cggccctctg tggacggaat cttggagaga
     1741 ctgtctgcgg ttgaagaatc cacggacaag aaggtgtaag aggacaaagt ggacatttgt
     1801 ccttcatatc tgttagcagc atcaacttat tgctgggagc aaatagcttc tttctccctt
     1861 gcttgcttgc tttttttctt tcttttactt gagatgggat ctcaccatgt agcccctgaa
     1921 aactgtgtag aacaggctgg ctcaaactca aggaaatctt ccagttctgc ctcctgaatg
     1981 ctggaattaa aggcatagac caccaaa
//
