LOCUS       NM_175337               5533 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus mutL homolog 3 (Mlh3), transcript variant 1, mRNA.
ACCESSION   NM_175337 XM_900622 XM_900639 XM_900643 XM_920990 XM_921009
            XM_921016 XM_994145 XM_994168 XM_994183
VERSION     NM_175337.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Li,Y., Wu,Y., Khan,I., Zhou,J., Lu,Y., Ye,J., Liu,J., Xie,X.,
            Hu,C., Jiang,H., Fan,S., Zhang,H., Zhang,Y., Jiang,X., Xu,B., Ma,H.
            and Shi,Q.
  TITLE     M1AP interacts with the mammalian ZZS complex and promotes male
            meiotic recombination
  JOURNAL   EMBO Rep 24 (2), e55778 (2023)
   PUBMED   36440627
REFERENCE   3  (bases 1 to 5533)
  AUTHORS   Goold,R., Hamilton,J., Menneteau,T., Flower,M., Bunting,E.L.,
            Aldous,S.G., Porro,A., Vicente,J.R., Allen,N.D., Wilkinson,H.,
            Bates,G.P., Sartori,A.A., Thalassinos,K., Balmus,G. and
            Tabrizi,S.J.
  TITLE     FAN1 controls mismatch repair complex assembly via MLH1 retention
            to stabilize CAG repeat expansion in Huntington's disease
  JOURNAL   Cell Rep 36 (9), 109649 (2021)
   PUBMED   34469738
REFERENCE   4  (bases 1 to 5533)
  AUTHORS   Singh,P., Fragoza,R., Blengini,C.S., Tran,T.N., Pannafino,G.,
            Al-Sweel,N., Schimenti,K.J., Schindler,K., Alani,E.A., Yu,H. and
            Schimenti,J.C.
  TITLE     Human MLH1/3 variants causing aneuploidy, pregnancy loss, and
            premature reproductive aging
  JOURNAL   Nat Commun 12 (1), 5005 (2021)
   PUBMED   34408140
  REMARK    GeneRIF: Human MLH1/3 variants causing aneuploidy, pregnancy loss,
            and premature reproductive aging.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5533)
  AUTHORS   Hayward,B.E., Steinbach,P.J. and Usdin,K.
  TITLE     A point mutation in the nuclease domain of MLH3 eliminates repeat
            expansions in a mouse stem cell model of the Fragile X-related
            disorders
  JOURNAL   Nucleic Acids Res 48 (14), 7856-7863 (2020)
   PUBMED   32619224
  REMARK    GeneRIF: A point mutation in the nuclease domain of MLH3 eliminates
            repeat expansions in a mouse stem cell model of the Fragile
            X-related disorders.
REFERENCE   6  (bases 1 to 5533)
  AUTHORS   Zeng,F., Baldwin,D.A. and Schultz,R.M.
  TITLE     Transcript profiling during preimplantation mouse development
  JOURNAL   Dev Biol 272 (2), 483-496 (2004)
   PUBMED   15282163
REFERENCE   7  (bases 1 to 5533)
  AUTHORS   Kolas,N.K. and Cohen,P.E.
  TITLE     Novel and diverse functions of the DNA mismatch repair family in
            mammalian meiosis and recombination
  JOURNAL   Cytogenet Genome Res 107 (3-4), 216-231 (2004)
   PUBMED   15467367
  REMARK    Review article
REFERENCE   8  (bases 1 to 5533)
  AUTHORS   Lipkin,S.M., Moens,P.B., Wang,V., Lenzi,M., Shanmugarajah,D.,
            Gilgeous,A., Thomas,J., Cheng,J., Touchman,J.W., Green,E.D.,
            Schwartzberg,P., Collins,F.S. and Cohen,P.E.
  TITLE     Meiotic arrest and aneuploidy in MLH3-deficient mice
  JOURNAL   Nat Genet 31 (4), 385-390 (2002)
   PUBMED   12091911
  REMARK    GeneRIF: Mlh3 is required for Mlh1 binding to meiotic chromosomes
            and localizes to chromosomes from the mid pachynema stage of
            prophase. Mlh3(-/-) spermatocytes reach metaphase before succumbing
            to apoptosis, oocytes fail to complete meiosis I after
            fertilization.
REFERENCE   9  (bases 1 to 5533)
  AUTHORS   Santucci-Darmanin,S., Neyton,S., Lespinasse,F., Saunieres,A.,
            Gaudray,P. and Paquis-Flucklinger,V.
  TITLE     The DNA mismatch-repair MLH3 protein interacts with MSH4 in meiotic
            cells, supporting a role for this MutL homolog in mammalian meiotic
            recombination
  JOURNAL   Hum Mol Genet 11 (15), 1697-1706 (2002)
   PUBMED   12095912
  REMARK    GeneRIF: Associated with MSH4 in mammalian meiotic cells,
            supporting the possibility that MLH3 plays a role in meiotic
            recombination.
REFERENCE   10 (bases 1 to 5533)
  AUTHORS   Lipkin,S.M., Wang,V., Jacoby,R., Banerjee-Basu,S., Baxevanis,A.D.,
            Lynch,H.T., Elliott,R.M. and Collins,F.S.
  TITLE     MLH3: a DNA mismatch repair gene associated with mammalian
            microsatellite instability
  JOURNAL   Nat Genet 24 (1), 27-35 (2000)
   PUBMED   10615123
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK035887.1, BC079861.1 and BE993968.1.
            
            On Jan 28, 2015 this sequence version replaced NM_175337.1.
            
            Transcript Variant: This variant (1) is the longer transcript. Both
            variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079861.1, SRR9219382.22713.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AK035887.1         2-36
            36-5463             BC079861.1         1-5428
            5464-5533           BE993968.1         1-70                c
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 39.61 cM"
     gene            1..5533
                     /gene="Mlh3"
                     /note="mutL homolog 3"
                     /db_xref="GeneID:217716"
                     /db_xref="MGI:MGI:1353455"
     exon            1..61
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            62..3384
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..4361
                     /gene="Mlh3"
                     /note="mutL 3"
                     /codon_start=1
                     /product="DNA mismatch repair protein Mlh3"
                     /protein_id="NP_780546.1"
                     /db_xref="CCDS:CCDS36497.1"
                     /db_xref="GeneID:217716"
                     /db_xref="MGI:MGI:1353455"
                     /translation="MIRCLSDDVKTKLRSGLAISSLGQCVEELTLNSIDAEATCVAIR
                     VNMETFQVQVIDNGLGMAGDDVEKVGNRYFTSKCHSVRDLENPAFYGFRGEALASIAD
                     MAGAVEISSKKNTTLKTFVKMFQNGKALATHEADLTRPSVGTTVTVYNLFYQFPVRRK
                     SMDPRLEFEKVRQRVEALSLMHPSISFSLRNDVSGSMVLQLPKTKDICSRFCQIYGLG
                     KSQKLREIRFKYKEFEFSGYISSEAHYNKNMQFLFVNRRLVLRTKLHKLIDFLLRKES
                     IICRPKNGSASRQMNSSPRHRSASELHGIYVINVQCPFCEYDVCIEPAKTLIEFQSWD
                     TVLICIQEGVKRFLKQEKLFVELSGEDIKEFNEDNGFSLFGTTLQTHVSTHEKCDQSS
                     FREACNKILDSYEMFNLQSKAVKRIATLENKTRQNPGDSETIRKKTVGSLYTDASDGP
                     CYSKSVESVLQDSNNSAYLEPRVSEEEVAKTSHSGENEKWKKSFLENKTSGRIHETSP
                     KMFSSPIQMHHLLEEREADLEMQTISSTVNVMAANIPQNNDIPSQLEKWKDAPEVGCQ
                     PLPFETTLLRVRGTQRKKERRKKEPSSRGRVNVFSYGQVKLCSTGFITHVVQSEHAKS
                     TETEHSFKNYARPGPVSAQETFGKRTHHAIETPDSSDLTSTLSKESSQPPNKRFCRTN
                     TGYGTENKPVATDDNLALFQESCKESHTDRLLPDASSFPWCRYVSDGCRKIDKRGSFK
                     QVVRRKLSLRSQVGSLEKFKRQYGKVSSSLDTEKDNNTEVRTHLDPQNEPDVLLKDKS
                     HLDMSDGCEITTVEHSETCQPLSPILYPEKILFSKEDRLEQMPHLRESPITLEELSHC
                     NRKADVEKSAASLASKLSKLKDSEKEMQTVGMTGHTSELPDSNPSWKDNSQCTRLDLD
                     FCELLKNKLEKIESDMLPMADSATEDGPINKNSELHPNNTTDDTEKPETPLLFPCNDS
                     KISRDSDVLIRTSEQPTGNPDSVGKVIMSQVEDGIGSQGGVCPQGDESKARSCSKNEP
                     NAHCMDWQQHFDVTLGRMVYINRMTGLSTFVAPTDDLHTACTKDLTTVAVDVLLGNDA
                     VDAAAAAVSEPLQSLFSEWSNPVFARYPEVAVDVSSGQAESLAVKIHNVLYPYRFTKE
                     MIHSVKVLQQVDNKFIACLMSTRMDEDGRTGGNLLVLVDQHAAHERIRLEQLITDSYE
                     KQDPQSAGRKKLLSSTIIPPLAITVSEEQRRLLRSYHKHLEDLGLELLFPDASDSLIL
                     VGKVPLCFVEREASELRRGRSTVTKSIVEELIREQVELLQTTGGIQGTLPLTVQKVLA
                     SQACHGAIKFNDRLSLEESCRLIEALSLSQLPFQCAHGRPSMLPLADLDHLEQEKQVK
                     PNLAKLRKMVRAWHLFGKTEQNLQQPIRPCEPP"
     exon            3385..3476
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3477..3581
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3582..3654
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3655..3726
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3727..3838
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3839..3998
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..4022
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            4023..4101
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            4102..4253
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     exon            4254..5517
                     /gene="Mlh3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5444..5449
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mlh3"
                     /note="hexamer: AATAAA"
     polyA_site      5463
                     /gene="Mlh3"
                     /note="major polyA site"
     regulatory      5480..5485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mlh3"
                     /note="hexamer: AATGAA"
     polyA_site      5510
                     /gene="Mlh3"
ORIGIN      
        1 gggagcgcgc gctcggcgtt ggaggtggtt ggtcggtgag agttgatgga ggagattcga
       61 ggtgattatt ttccagtcag agaaggaagc cagtgtctgc cactccctcc acatgtgtcc
      121 ctgccatgat caggtgtcta tcagatgacg taaaaaccaa gttgcgttcc ggtttagcca
      181 taagctcctt gggccagtgt gttgaagaac ttacccttaa cagtattgat gctgaagcaa
      241 catgtgtggc catcagagtg aatatggaaa ccttccaagt tcaagtgata gacaatggac
      301 ttgggatggc gggggacgat gtagagaagg tgggaaaccg gtattttact agtaaatgcc
      361 actcagtgcg ggacttggag aacccagcat tttatggctt ccgaggagag gccttggcaa
      421 gtatagccga catggctggt gctgtggaga tttcatccaa gaaaaacaca acactgaaaa
      481 cctttgtgaa aatgtttcag aatggaaaag cccttgccac ccatgaggct gatttgacca
      541 gaccaagtgt ggggactaca gtaacggtct ataacctgtt ttaccagttt cctgtgcgga
      601 ggaaaagcat ggatcctaga ctagagtttg agaaagttcg gcagagggta gaagctctct
      661 cacttatgca cccctccatt tctttctctt tgaggaacga tgtatctgga tccatggttc
      721 ttcagctccc taaaaccaaa gacatatgct ctcgattctg tcaaatttac ggattgggca
      781 agtcccaaaa gttaagagaa atacgtttta aatacaagga atttgagttc agtggctaca
      841 tcagctctga agcacactac aataagaata tgcagttttt gtttgtgaac agaagactag
      901 ttttaagaac aaagttgcat aaacttattg actttttatt aagaaaagaa agcattatat
      961 gcaggccaaa gaatggctct gccagtaggc aaatgaattc aagtcctcga caccgttctg
     1021 cctcagaact ccacgggata tatgtaatca atgtgcagtg ccctttctgt gagtatgatg
     1081 tctgcataga gccagccaaa actctgattg agtttcagag ctgggatacc gtgttgattt
     1141 gtattcagga aggagtaaaa aggtttttaa agcaagaaaa attatttgta gaattatcag
     1201 gtgaagatat taaggaattt aatgaagata atggttttag tttgtttggc acgactcttc
     1261 agacacatgt gtctactcat gagaagtgtg accagagcag tttccgggaa gcgtgtaata
     1321 aaattctgga ttcctatgaa atgtttaatt tgcagtcaaa agctgtgaaa agaatagcta
     1381 ctctagaaaa taaaaccaga caaaaccctg gtgattcaga aactatcaga aaaaagacag
     1441 tgggctcatt gtacacagat gcatcggatg gcccgtgcta tagtaaatcg gtagagtctg
     1501 ttttacagga cagcaacaac agtgcttact tagaaccgag ggtgtcagaa gaagaggtag
     1561 ccaaaacatc acactccgga gaaaatgaga aatggaaaaa atcttttttg gaaaataaga
     1621 cttcaggaag gatacatgaa accagtccaa aaatgttttc aagccccatc caaatgcatc
     1681 acctccttga ggagagagag gcagatctgg aaatgcagac aataagtagt actgttaatg
     1741 tcatggctgc caacattccc caaaataatg acattccgag tcaactggag aaatggaaag
     1801 atgctcctga agtggggtgc caacctctgc cttttgagac aaccttatta agggtacggg
     1861 gtactcagag gaagaaggaa agaaggaaaa aggagcccag tagtcgtgga agagtaaatg
     1921 tttttagtta tggacaagtt aaattatgct ccactggctt tataactcat gtagtacaaa
     1981 gtgagcacgc taaatcaact gaaacagaac attcatttaa aaattatgct cgacctggtc
     2041 ctgtaagtgc ccaagaaaca tttggaaaaa gaacacacca tgcaattgag actccagaca
     2101 gcagtgattt aacaagcact ttaagtaaag aatccagtca accgcccaac aaaaggtttt
     2161 gcagaacaaa tacaggttac gggacagaga acaaacctgt agcaacagat gacaacttgg
     2221 ctctttttca ggaaagctgt aaagaatcac acacagatcg ccttttgcct gatgcatcct
     2281 ccttcccatg gtgtagatat gtttccgatg gttgtaggaa aatagataaa aggggttcct
     2341 tcaaacaggt agtccgtagg aagctaagct tgcgttcaca agtagggtct ttagagaagt
     2401 ttaagaggca gtatgggaag gtcagcagtt ccctagatac agaaaaggat aataacactg
     2461 aagtcaggac tcatcttgat cctcaaaatg aacccgatgt tcttctgaaa gacaagagcc
     2521 acttagatat gtctgatggt tgtgagatca ctactgtgga gcacagtgag acttgtcaac
     2581 cattaagtcc catcctgtac ccagaaaaaa ttttattttc caaagaagat cgcttagaac
     2641 agatgcctca tttgagagaa agtcctataa ctctggaaga attatctcac tgtaacagaa
     2701 aagctgatgt tgagaagtcc gctgcatcac tggcttctaa attatccaaa ctaaaggatt
     2761 ctgaaaaaga gatgcaaacc gtggggatga caggtcatac tagtgaactt ccagattcaa
     2821 atcccagttg gaaagataat agccagtgca ctaggttaga cttggatttt tgtgaattat
     2881 taaaaaacaa acttgaaaaa atagagagtg atatgcttcc aatggcagat tctgccacag
     2941 aggatggtcc catcaataaa aacagtgaac tacatcctaa caatacaacg gatgacacag
     3001 agaaaccaga aactcctttg ctgttcccct gtaatgattc taaaatcagc agagattcag
     3061 atgttcttat cagaacttca gaacaaccta caggaaaccc tgactctgtc ggtaaagtga
     3121 taatgagtca ggtagaggat ggcattggca gccaaggtgg agtctgtccc cagggtgatg
     3181 aatctaaggc aagatcttgt tccaaaaatg aaccaaacgc acactgtatg gattggcagc
     3241 agcattttga tgtaaccctg ggaagaatgg tttacatcaa cagaatgaca ggacttagca
     3301 cattcgttgc tccaactgac gaccttcata ctgcttgtac taaagatctg acaactgtgg
     3361 ctgtggatgt cctgcttggg aatgatgctg tggatgctgc tgctgctgct gtcagtgaac
     3421 cccttcagtc tctgttttca gaatggagca atccagtgtt tgctcgatac ccagaggttg
     3481 ctgttgatgt cagcagtggc caggctgaga gcttagccgt taaaattcac aacgtcctgt
     3541 atccctatcg cttcaccaaa gagatgattc actcagtgaa ggttctccag caagtggata
     3601 acaagtttat tgcctgctta atgagcacga ggatggatga ggatggccga acaggtggaa
     3661 acctgttagt cctggtggac cagcatgctg cccatgaacg cattcgtttg gagcagctta
     3721 ttactgattc ctatgagaaa caagatccac aaagcgctgg ccggaagaaa ttattgtctt
     3781 ccacaataat ccctccactg gcaatcaccg tgtcagagga acaaaggaga ctcttacggt
     3841 cttaccacaa acatttagaa gatctggggc ttgagttgct ctttccagat gctagtgatt
     3901 ctctgatcct ggtgggaaaa gtgccgctct gctttgtaga gagagaagct agtgagcttc
     3961 gaagaggacg ctctactgtg actaagagta ttgtggagga attaattcga gaacaagtgg
     4021 agctgctcca gaccacagga ggtatccaag ggacactgcc actgactgtc cagaaggtgt
     4081 tggcctccca ggcctgccat ggggctatta agtttaatga tcgtctgagc ctagaagaga
     4141 gctgccgcct catcgaagct ctgtccttgt cccagctgcc atttcagtgt gctcatggga
     4201 gaccctcaat gctgccctta gctgacctgg accacttgga gcaggaaaaa caggttaaac
     4261 ccaaccttgc taaacttcgc aaaatggttc gtgcctggca tctctttgga aaaacagagc
     4321 agaacctgca gcagcctata cgtccttgtg agcctccatg aggagaggat tctggagtgt
     4381 aaggagacaa gggagtgccg tgcatcccga gcaggagcag tgcagctgtg ggcaggtcgg
     4441 cggccctgag cgggctggca catcagtccc cctgagcaga tggagcaggc acgtgcactc
     4501 aagcctaagg gtagtttatt tctttgcatc catgcacaca ggagcttgac atataatacc
     4561 tatcttttgt aagttgattt agtgataaaa tgtaatgatt ttgtaattgg tgagttggct
     4621 tatgtttgag gggcgcagct attgttttta gcagttttcc ccagcctctc agtttatatt
     4681 acgtgaggat gctaagccct aagcgctggt ctgctcttct ctgagcccct ggctctgccc
     4741 ctccccatcc atttctcttt tgcatttgtc tccttcactt catacctctg cttcttcaca
     4801 ttgtgcttta cagacttacg gtgtttctct gctcattata aaaatatttc ccgccaggca
     4861 gcggtggctc acacctttag tcccagtact tgggaggcag aggcaggtgg atttctgatt
     4921 tcaaggccag cctggtctgc aaagtgagtt ccaggacagc cacagggcta caaagagaaa
     4981 ccctgtctca aaaaaaccaa aacaacaaca acaacaacaa aacttcctga tgtcttccag
     5041 agagactaaa ttatattagg gattaaaagt tatttatagc tgggtgtagt gacatatacc
     5101 tgtaatccta gtacctggga ggctgagaca agtccgtgac agggcaccat ttgcctaggc
     5161 tggatatata gcaagacctt gactcaaaat aaataagtaa actatagaca aagagagaca
     5221 caaagacaga atagagaaag tttgaaaaga atttttttaa ttctcttggt aacctggctg
     5281 tcctggaact ggaactcaga gatccccctt tgctgcctcc gagtgctggg attaacggtg
     5341 cgcgccacca ctgcctggca agaaaaatta caaccctgtc cctggttttt ttgatagtct
     5401 tactggtttt taaaaccaga ggtgaatgtt tctattctga actaataaaa cactaaaaaa
     5461 taatgggttt gtttttgaaa atgaatttgt actttataaa atgcttctgt gaaattaaaa
     5521 aaaaaaaaaa aaa
//
