LOCUS       NM_011746               2561 bp    mRNA    linear   ROD 16-FEB-2025
DEFINITION  Mus musculus makorin, ring finger protein, 3 (Mkrn3), mRNA.
ACCESSION   NM_011746
VERSION     NM_011746.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Pereira,S.A., Oliveira,F.C.B., Naule,L., Royer,C., Neves,F.A.R.,
            Abreu,A.P., Carroll,R.S., Kaiser,U.B., Coelho,M.S. and
            Lofrano-Porto,A.
  TITLE     Mouse Testicular Mkrn3 Expression Is Primarily Interstitial,
            Increases Peripubertally, and Is Responsive to LH/hCG
  JOURNAL   Endocrinology 164 (9) (2023)
   PUBMED   37585624
  REMARK    GeneRIF: Mouse Testicular Mkrn3 Expression Is Primarily
            Interstitial, Increases Peripubertally, and Is Responsive to
            LH/hCG.
REFERENCE   3  (bases 1 to 2561)
  AUTHORS   Naule,L., Mancini,A., Pereira,S.A., Gassaway,B.M., Lydeard,J.R.,
            Magnotto,J.C., Kim,H.K., Liang,J., Matos,C., Gygi,S.P.,
            Merkle,F.T., Carroll,R.S., Abreu,A.P. and Kaiser,U.B.
  TITLE     MKRN3 inhibits puberty onset via interaction with IGF2BP1 and
            regulation of hypothalamic plasticity
  JOURNAL   JCI Insight 8 (8), e164178 (2023)
   PUBMED   37092553
  REMARK    GeneRIF: MKRN3 inhibits puberty onset via interaction with IGF2BP1
            and regulation of hypothalamic plasticity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2561)
  AUTHORS   Fanis,P., Morrou,M., Tomazou,M., Michailidou,K., Spyrou,G.M.,
            Toumba,M., Skordis,N., Neocleous,V. and Phylactou,L.A.
  TITLE     Methylation status of hypothalamic Mkrn3 promoter across puberty
  JOURNAL   Front Endocrinol (Lausanne) 13, 1075341 (2023)
   PUBMED   36714607
  REMARK    GeneRIF: Methylation status of hypothalamic Mkrn3 promoter across
            puberty.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2561)
  AUTHORS   Roberts,S.A., Naule,L., Chouman,S., Johnson,T., Johnson,M.,
            Carroll,R.S., Navarro,V.M. and Kaiser,U.B.
  TITLE     Hypothalamic Overexpression of Makorin Ring Finger Protein 3
            Results in Delayed Puberty in Female Mice
  JOURNAL   Endocrinology 163 (11) (2022)
   PUBMED   35974456
  REMARK    GeneRIF: Hypothalamic Overexpression of Makorin Ring Finger Protein
            3 Results in Delayed Puberty in Female Mice.
REFERENCE   6  (bases 1 to 2561)
  AUTHORS   Jong,M.T., Carey,A.H., Caldwell,K.A., Lau,M.H., Handel,M.A.,
            Driscoll,D.J., Stewart,C.L., Rinchik,E.M. and Nicholls,R.D.
  TITLE     Imprinting of a RING zinc-finger encoding gene in the mouse
            chromosome region homologous to the Prader-Willi syndrome genetic
            region
  JOURNAL   Hum Mol Genet 8 (5), 795-803 (1999)
   PUBMED   10196368
  REMARK    GeneRIF: Mkrn3, encoding a RING zinc-finger protein, is imprinted,
            with preferential expression from the paternal allele.
REFERENCE   7  (bases 1 to 2561)
  AUTHORS   Cattanach,B.M., Barr,J.A., Beechey,C.V., Martin,J., Noebels,J. and
            Jones,J.
  TITLE     A candidate model for Angelman syndrome in the mouse
  JOURNAL   Mamm Genome 8 (7), 472-478 (1997)
   PUBMED   9195990
  REMARK    Erratum:[Mamm Genome 1997;8(11):877]
REFERENCE   8  (bases 1 to 2561)
  AUTHORS   Toder,R., Wilcox,S.A., Smithwick,M. and Graves,J.A.
  TITLE     The human/mouse imprinted genes IGF2, H19, SNRPN and ZNF127 map to
            two conserved autosomal clusters in a marsupial
  JOURNAL   Chromosome Res 4 (4), 295-300 (1996)
   PUBMED   8817070
REFERENCE   9  (bases 1 to 2561)
  AUTHORS   Johnson,D.K., Stubbs,L.J., Culiat,C.T., Montgomery,C.S.,
            Russell,L.B. and Rinchik,E.M.
  TITLE     Molecular analysis of 36 mutations at the mouse pink-eyed dilution
            (p) locus
  JOURNAL   Genetics 141 (4), 1563-1571 (1995)
   PUBMED   8601494
REFERENCE   10 (bases 1 to 2561)
  AUTHORS   Nicholls,R.D., Gottlieb,W., Russell,L.B., Davda,M., Horsthemke,B.
            and Rinchik,E.M.
  TITLE     Evaluation of potential models for imprinted and nonimprinted
            components of human chromosome 15q11-q13 syndromes by
            fine-structure homology mapping in the mouse
  JOURNAL   Proc Natl Acad Sci U S A 90 (5), 2050-2054 (1993)
   PUBMED   8095339
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156555.8.
            
            On Jun 5, 2018 this sequence version replaced NM_011746.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK134870.1, BC054771.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 10196368
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2561              AC156555.8         235331-237891       c
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 34.37 cM"
     gene            1..2561
                     /gene="Mkrn3"
                     /gene_synonym="D7H15S9-1; Zfp127"
                     /note="makorin, ring finger protein, 3"
                     /db_xref="GeneID:22652"
                     /db_xref="MGI:MGI:2181178"
     exon            1..2561
                     /gene="Mkrn3"
                     /gene_synonym="D7H15S9-1; Zfp127"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..1747
                     /gene="Mkrn3"
                     /gene_synonym="D7H15S9-1; Zfp127"
                     /EC_number="2.3.2.27"
                     /note="probable E3 ubiquitin-protein ligase makorin-3;
                     zinc finger protein 127; RING-type E3 ubiquitin
                     transferase makorin-3"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase makorin-3"
                     /protein_id="NP_035876.2"
                     /db_xref="CCDS:CCDS21327.1"
                     /db_xref="GeneID:22652"
                     /db_xref="MGI:MGI:2181178"
                     /translation="MEESTAPIEAHAAAGAEAGAEGGEGVSVPPPPQFEAAGASAGVS
                     SAPLQQASGLAPLLVTPGPAIRRAASLRPAPAEGGGARSGPERNSGSWTKQILCRYYL
                     HGQCKEGDNCRYSHDLSGRRRSRGGQDAQPRASADRGPKMATRWEPPTQEVAEAPPAA
                     SSSSLPLIGSAAERGFTEAEIDNAGIRSAAERGFSEAEIDNASLAAGAAAGAGAEGWE
                     GAIEFVPGQPYRGRMVPPHGPEAPLQSPAIEREHMAMGMGMPMPVPMPMPVPMPVPMP
                     LPLCRYAARGQCLRGDRCAYPHGEICDMCGQQALHPWDAAQQEAHRRACVEAHERDME
                     LSFAVQRSMDKVCGICMEVVYEKADPSDRRFGILFSCNHTYCLRCIRRWRSATQFENR
                     ISKSCPQCRVSSGFVIPSEFWVEEEEEKEKLVQQYKEGMSQKACRYFAGGLGHCPFGE
                     FCFYKHEYPEGWRDQPPRPDGGGSSSAYWHQVLEPVQLREGNVLFKSRKKEHSVLRLA
                     NQLLKKLLCLRGSSSFSDDRWLLLQYQLEEYFSLNL"
     regulatory      2539..2544
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mkrn3"
                     /gene_synonym="D7H15S9-1; Zfp127"
                     /note="hexamer: AATAAA"
     polyA_site      2561
                     /gene="Mkrn3"
                     /gene_synonym="D7H15S9-1; Zfp127"
                     /note="major polyA site"
ORIGIN      
        1 gatgcacttc gctccgagaa gaagctggtc gagccatctc cagctccgca attcaagcac
       61 aaaaaagata taatattcgg gtttttggac agtgttagag gcacgctgta agatggaaga
      121 gtctacagct cccattgagg cccacgcggc cgccggcgcc gaggcaggtg ctgaaggggg
      181 ggagggtgtg tctgttcccc cccctcctca gtttgaggct gctggagctt ctgcgggagt
      241 cagctcggcc cctctgcagc aggcatcggg tctggcaccg ctcctggtga ctccaggccc
      301 agcgatccgg cgcgcggcga gtttgaggcc agcgccagcc gaagggggcg gggcccggtc
      361 tggtccggag cgcaacagtg gcagctggac gaagcaaatc ctctgcaggt attatctgca
      421 tgggcagtgc aaggagggcg ataactgtcg ctactctcac gacctttctg ggcggcggag
      481 atctaggggg gggcaagatg ctcagcctcg ggcctctgca gacagaggac ccaagatggc
      541 cactcgctgg gagcccccga ctcaggaagt ggcggaagcc ccccctgctg catcctcaag
      601 ctccttgcct ctgattggct cggctgctga aaggggcttc actgaagctg agattgacaa
      661 tgcaggcatt cgctcagctg ctgaaagggg cttctctgaa gccgagattg acaatgcaag
      721 ccttgcagca ggtgctgcag cgggagcggg tgcagaaggc tgggaggggg caattgaatt
      781 tgttcctgga cagccttacc gaggtcgcat ggtccccccg catggtcccg aggctcctct
      841 gcagagcccg gcaattgaga gagagcatat ggctatgggc atggggatgc cgatgccagt
      901 gccaatgcca atgccagtgc caatgccagt gccaatgccg ctgccgcttt gccgctatgc
      961 tgcccgggga caatgcctcc gtggggacag gtgtgcatac ccccatggag aaatatgcga
     1021 catgtgcggg cagcaggcct tgcacccttg ggatgcagct cagcaggaag ctcatagaag
     1081 ggcctgcgtt gaagcacacg agagagatat ggaactctct tttgctgtgc agcgcagcat
     1141 ggacaaagtg tgtggcatct gcatggaggt ggtctatgag aaagctgacc ccagcgaccg
     1201 ccgctttggc attcttttca gctgcaacca cacctactgt cttaggtgta tccgcaggtg
     1261 gagaagtgcc acacagtttg agaacaggat cagtaagtcc tgcccacagt gcagggtctc
     1321 ctctggcttt gtcattccta gtgagttttg ggtggaggag gaagaggaga aggagaaact
     1381 tgttcagcag tataaggagg ggatgagcca gaaagcgtgc aggtattttg ctggaggcct
     1441 aggtcactgc ccatttggag aattctgttt ttacaagcat gaataccccg agggctggag
     1501 agatcagcct ccaagaccag atggtggtgg atcatcaagc gcatactggc atcaagtttt
     1561 ggagcctgtg cagttgcgag agggaaacgt gctgtttaaa agccgtaaaa aagagcattc
     1621 tgtgcttcgc ctggccaatc agttgcttaa gaagttgctt tgcctgagag gcagttcttc
     1681 cttctctgat gaccgttggc tcttgcttca gtaccagctg gaagaatatt tcagtttgaa
     1741 tctgtagtat cacgctgtgg catgtggtct ggtctgctaa agctctgttg tctgctgttg
     1801 cttattcccc tttattgact gaaaaaccct atcgctttca aaggctactg aggcaatctt
     1861 tatgcagtat tgctgttttc ttctctattt cctatttctt cttgctagga ttgtgatgtt
     1921 tctctgtgtt acaaaataac aagaaatctt ataagtattg tttggtggaa ttaatatttc
     1981 tgtcaactga tgacttgtga tgtctttttt ccaacaggat tcaatcactt ctagtctagt
     2041 gtttacagtt tctcattgtg aagtccctta agagtaaatg tgacaaggtg acaaaagtct
     2101 ttaactgaat tatgagcttt ttgctatata gccttaaaaa aaatggaccc ttacaggacc
     2161 ttgcagctgc tgtctgtgtt tgtttacatg tttgtttctt cccttctctt tcaagtgcac
     2221 ttgttaactt gtggttactt tgtggttctg ttttacttga ccaaaatgag gtgcatatgt
     2281 ttacatgttt ttatcctgtt tggcttgatg tgaaattatt tatatttgga aatatatatt
     2341 taagaatgat atatatgcgt atatgtatac aaagatgtat ttgaaaaaaa ctggaaataa
     2401 atatacacca taatatatat gtttgaagta ttgaaataaa tgcagaactg aaagcccagt
     2461 gttaaagtcc aagtgtgtgg gctgtatgtg aattgtgtgg ataatctgcc cctgcactga
     2521 tgtcagtgat tagcatctaa taaaaatgac tgctaagtgt g
//
