LOCUS       NM_023290               2240 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus makorin, ring finger protein, 2 (Mkrn2), mRNA.
ACCESSION   NM_023290
VERSION     NM_023290.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wang,L., Yong,Y.L., Wang,K.K., Xie,Y.X., Qian,Y.C., Zhou,F.M.,
            Qiu,J.G. and Jiang,B.H.
  TITLE     MKRN2 knockout causes male infertility through decreasing STAT1,
            SIX4, and TNC expression
  JOURNAL   Front Endocrinol (Lausanne) 14, 1138096 (2023)
   PUBMED   36967804
  REMARK    GeneRIF: MKRN2 knockout causes male infertility through decreasing
            STAT1, SIX4, and TNC expression.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2240)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2240)
  AUTHORS   Qian,Y.C., Xie,Y.X., Wang,C.S., Shi,Z.M., Jiang,C.F., Tang,Y.Y.,
            Qian,X., Wang,L. and Jiang,B.H.
  TITLE     Mkrn2 deficiency induces teratozoospermia and male infertility
            through p53/PERP-mediated apoptosis in testis
  JOURNAL   Asian J Androl 22 (4), 414-421 (2020)
   PUBMED   31489847
  REMARK    GeneRIF: Mkrn2 deficiency induces teratozoospermia and male
            infertility through p53/PERP-mediated apoptosis in testis.
REFERENCE   5  (bases 1 to 2240)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2240)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   7  (bases 1 to 2240)
  AUTHORS   Wiese,C.B., Ireland,S., Fleming,N.L., Yu,J., Valerius,M.T.,
            Georgas,K., Chiu,H.S., Brennan,J., Armstrong,J., Little,M.H.,
            McMahon,A.P. and Southard-Smith,E.M.
  TITLE     A genome-wide screen to identify transcription factors expressed in
            pelvic Ganglia of the lower urinary tract
  JOURNAL   Front Neurosci 6, 130 (2012)
   PUBMED   22988430
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 2240)
  AUTHORS   Guo,G., Huss,M., Tong,G.Q., Wang,C., Li Sun,L., Clarke,N.D. and
            Robson,P.
  TITLE     Resolution of cell fate decisions revealed by single-cell gene
            expression analysis from zygote to blastocyst
  JOURNAL   Dev Cell 18 (4), 675-685 (2010)
   PUBMED   20412781
REFERENCE   9  (bases 1 to 2240)
  AUTHORS   Yang,P.H., Cheung,W.K., Peng,Y., He,M.L., Wu,G.Q., Xie,D.,
            Jiang,B.H., Huang,Q.H., Chen,Z., Lin,M.C. and Kung,H.F.
  TITLE     Makorin-2 is a neurogenesis inhibitor downstream of
            phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal
  JOURNAL   J Biol Chem 283 (13), 8486-8495 (2008)
   PUBMED   18198183
REFERENCE   10 (bases 1 to 2240)
  AUTHORS   Gray,T.A., Azama,K., Whitmore,K., Min,A., Abe,S. and Nicholls,R.D.
  TITLE     Phylogenetic conservation of the makorin-2 gene, encoding a
            multiple zinc-finger protein, antisense to the RAF1 proto-oncogene
  JOURNAL   Genomics 77 (3), 119-126 (2001)
   PUBMED   11597136
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160032.7.
            
            On Aug 14, 2018 this sequence version replaced NM_023290.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077926.1, AK011295.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-76                AC160032.7         109384-109459       c
            77-205              AC160032.7         101688-101816       c
            206-387             AC160032.7         100697-100878       c
            388-692             AC160032.7         99458-99762         c
            693-907             AC160032.7         97856-98070         c
            908-1018            AC160032.7         96712-96822         c
            1019-1163           AC160032.7         93955-94099         c
            1164-2240           AC160032.7         92727-93803         c
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.61 cM"
     gene            1..2240
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /note="makorin, ring finger protein, 2"
                     /db_xref="GeneID:67027"
                     /db_xref="MGI:MGI:1914277"
     exon            1..76
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1301
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /EC_number="2.3.2.27"
                     /note="probable E3 ubiquitin-protein ligase makorin-2;
                     Makorin RING zinc-finger protein 2; RING-type E3 ubiquitin
                     transferase makorin-2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase makorin-2"
                     /protein_id="NP_075779.2"
                     /db_xref="CCDS:CCDS39600.1"
                     /db_xref="GeneID:67027"
                     /db_xref="MGI:MGI:1914277"
                     /translation="MSTKQVTCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGY
                     CAYGARCRYDHTKPPAAAGGAVGPAPNPSPSSGLHSPHPSPDIATSVMRTHSNEPGKR
                     EKKTLVLRDRNLTGLAEDKTPPSKVNNPGGCSDPQTSPEMKPHSYLDAIRTGLDDLEA
                     SSSYSNEPQLCPYAAAGECRFGDACVYLHGDMCEICRLQVLHPFDPEQRKAHEKMCMS
                     TFEHEMEKAFAFQASQDKVCSICMEVILEKASASERRFGILSNCSHTYCLSCIRQWRC
                     AKQFENPIIKSCPECRVISEFVIPSVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGK
                     GTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSEGTVRFFNSVRLWDFIENRETRQVPS
                     TDDVDVTELGDLFMHLSGVESSEP"
     exon            77..205
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            206..387
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            388..692
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            693..907
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1018
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1163
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..2240
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1547..1552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /note="hexamer: AATGAA"
     polyA_site      1564
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /note="major polyA site"
     regulatory      1597..1602
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      1618
                     /gene="Mkrn2"
                     /gene_synonym="2610002L04Rik"
ORIGIN      
        1 gctggcggct ctgaggtttc agtgcgactc cagcccgtgg ccccgccgcc atgagcacca
       61 agcaggtcac ctgcaggtat tttatgcatg gcgtatgtcg cgaaggcagc cagtgcctgt
      121 tctcacatga cttggcgaac agcaagccgt ccaccatctg caaatactac cagaagggct
      181 actgcgccta tggtgcacgc tgcagatatg atcatacaaa gcccccagct gcagcaggtg
      241 gagctgtggg tcctgcaccc aacccttcgc cctcctcagg cctgcatagt cctcaccctt
      301 ctcctgacat tgccacatct gttatgagga cacattcgaa tgaacctgga aaacgggaga
      361 agaagacgct ggtgctcaga gaccgcaatc tcactggtct ggctgaagat aagactcctc
      421 caagcaaagt gaataatcca ggtggctgca gtgaccctca gaccagccca gagatgaagc
      481 cccattccta cctagatgcc atcaggactg gcctggatga cttggaggcc agcagctcct
      541 acagcaatga gccgcagttg tgcccctatg ctgccgctgg ggagtgtcgc tttggagatg
      601 cctgtgtcta cctgcatggg gacatgtgtg agatctgcag gcttcaagtc ctccacccct
      661 ttgacccaga acaaaggaaa gctcacgaga agatgtgtat gtcgacattt gaacatgaga
      721 tggaaaaggc gtttgccttc caggcgagcc aggacaaagt gtgcagcatc tgcatggagg
      781 tgatcctgga gaaagcatct gcttctgaaa ggaggtttgg cattctctcc aactgcagcc
      841 acacctactg cctgtcctgc atccgtcaat ggagatgtgc caagcagttt gaaaacccaa
      901 tcataaagtc ttgtccagag tgccgtgtga tatcagagtt tgtaattcca agtgtgtatt
      961 gggtagaaga tcagaataaa aagaatgaat taattgaagc tttcaaacag ggaatgggga
     1021 agaaggcatg caaatacttt gaacaaggca aggggacctg cccctttgga agcaagtgcc
     1081 tttaccgtca tgcttaccct gatgggcggc tggcagagcc agagaaacct cggaagcagc
     1141 taagctctga aggcactgtg aggttcttta actcagttcg gctttgggat tttatcgaga
     1201 accgagaaac ccggcaagtc cccagcactg acgatgtgga tgtgacagag ctaggagacc
     1261 tcttcatgca cctttctgga gtagaatcat cagaacccta gtcagtgctg acgccttcat
     1321 ttggctctgc agtcaacttt ccaaggcacg gtgtggacgc atctgtcacc gacccgaggt
     1381 gacgtggtac tgggatgtga gtgcagtagt gagtccccct gggaagggaa gggagatcac
     1441 cacgtccaca gaatcactac atttagagtt gagctctcag ttgtacctcc aacccctttc
     1501 tccgggacca gctatgtggt ccagctgttt taattgagtg cttttaaatg aaacctgact
     1561 gccatctttc ttttgtgact ttcttcctta agcaacagta aacttgcagc agatctggtt
     1621 tttttttttt ttccttgcca agacatcgcc agccctgtag aaggctcctc tgcagtgatc
     1681 atctctaata gagttgtcga gccttggaat tgatgcagct gtgtagtgag aacacatttg
     1741 gccatattgg tgctgctgta ccatagtgta gacttttaca gctgtgaggg tggctattga
     1801 gttccttcca gtgtctcaag tcacaggtgt gtgcagttgt gaagatctcc agggagactc
     1861 catcttaccc tccccttgta ctaagagcac aagacacagg actcacagat tgcttgttct
     1921 cccctataaa gtagtaggct ccacacttgt ttttgtggta aacaggagca gtcactcctc
     1981 tgtctaagaa ctgggggcaa gaaagagcaa atttgtcctg aacctgaatt tatttttata
     2041 agaaaacctg ttattacctg tgtgcttttc acttggttgt tcatgaactg cagagggggc
     2101 tccttgagtt ctgtctgcac aagaaactgc actgcaagtt ttgaaggagt cagtagccat
     2161 taaacctaaa tttaatttat tttattaaaa taacataagg aaccaactgt attggctcaa
     2221 taaaaacaaa attaaaaccc
//
