LOCUS       NM_018810               3037 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus makorin, ring finger protein, 1 (Mkrn1), transcript
            variant 1, mRNA.
ACCESSION   NM_018810
VERSION     NM_018810.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Lee,M.S., Han,H.J., Han,S.Y., Kim,I.Y., Chae,S., Lee,C.S.,
            Kim,S.E., Yoon,S.G., Park,J.W., Kim,J.H., Shin,S., Jeong,M., Ko,A.,
            Lee,H.Y., Oh,K.J., Lee,Y.H., Bae,K.H., Koo,S.H., Kim,J.W.,
            Seong,J.K., Hwang,D. and Song,J.
  TITLE     Loss of the E3 ubiquitin ligase MKRN1 represses diet-induced
            metabolic syndrome through AMPK activation
  JOURNAL   Nat Commun 9 (1), 3404 (2018)
   PUBMED   30143610
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Timmermans,S. and Libert,C.
  TITLE     Overview of inactivating mutations in the protein-coding genome of
            the mouse reference strain C57BL/6J
  JOURNAL   JCI Insight 3 (13), 121758 (2018)
   PUBMED   29997285
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3037)
  AUTHORS   Cassar,P.A., Carpenedo,R.L., Samavarchi-Tehrani,P., Olsen,J.B.,
            Park,C.J., Chang,W.Y., Chen,Z., Choey,C., Delaney,S., Guo,H.,
            Guo,H., Tanner,R.M., Perkins,T.J., Tenenbaum,S.A., Emili,A.,
            Wrana,J.L., Gibbings,D. and Stanford,W.L.
  TITLE     Integrative genomics positions MKRN1 as a novel ribonucleoprotein
            within the embryonic stem cell gene regulatory network
  JOURNAL   EMBO Rep 16 (10), 1334-1357 (2015)
   PUBMED   26265008
  REMARK    GeneRIF: Systems-level analyses support a role for the E3 ligase
            MKRN1 as a ribonucleoprotein within the embryonic stem cell gene
            regulatory network
REFERENCE   4  (bases 1 to 3037)
  AUTHORS   Kim,J.H., Park,K.W., Lee,E.W., Jang,W.S., Seo,J., Shin,S.,
            Hwang,K.A. and Song,J.
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and
            degradation prevents adipocyte differentiation
  JOURNAL   Cell Death Differ 21 (4), 594-603 (2014)
   PUBMED   24336050
  REMARK    GeneRIF: MKRN1 is an elusive PPARgamma E3 ligase that targets
            PPARgamma for proteasomal degradation by ubiquitin-dependent
            pathways, and further depict MKRN1 as a novel target for diseases
            involving PPARgamma.
REFERENCE   5  (bases 1 to 3037)
  AUTHORS   Ko,A., Shin,J.Y., Seo,J., Lee,K.D., Lee,E.W., Lee,M.S., Lee,H.W.,
            Choi,I.J., Jeong,J.S., Chun,K.H. and Song,J.
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated
            posttranslational regulation of p14ARF
  JOURNAL   J Natl Cancer Inst 104 (21), 1660-1672 (2012)
   PUBMED   23104211
REFERENCE   6  (bases 1 to 3037)
  AUTHORS   Wang,L., Zheng,A., Yi,L., Xu,C., Ding,M. and Deng,H.
  TITLE     Identification of potential nuclear reprogramming and
            differentiation factors by a novel selection method for cloning
            chromatin-binding proteins
  JOURNAL   Biochem Biophys Res Commun 325 (1), 302-307 (2004)
   PUBMED   15522233
REFERENCE   7  (bases 1 to 3037)
  AUTHORS   Hirotsune,S., Yoshida,N., Chen,A., Garrett,L., Sugiyama,F.,
            Takahashi,S., Yagami,K., Wynshaw-Boris,A. and Yoshiki,A.
  TITLE     An expressed pseudogene regulates the messenger-RNA stability of
            its homologous coding gene
  JOURNAL   Nature 423 (6935), 91-96 (2003)
   PUBMED   12721631
  REMARK    GeneRIF: Mutagenesis reduces transcription of Makorin1-pseudogene,
            resulting in destabilization of Makorin1 mRNA in trans by way of a
            cis-acting RNA decay element within the 5' region of Makorin1 that
            is homologous between Makorin1 and Makorin1-pseudogene
REFERENCE   8  (bases 1 to 3037)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 3037)
  AUTHORS   Gray,T.A., Azama,K., Whitmore,K., Min,A., Abe,S. and Nicholls,R.D.
  TITLE     Phylogenetic conservation of the makorin-2 gene, encoding a
            multiple zinc-finger protein, antisense to the RAF1 proto-oncogene
  JOURNAL   Genomics 77 (3), 119-126 (2001)
   PUBMED   11597136
REFERENCE   10 (bases 1 to 3037)
  AUTHORS   Gray,T.A., Hernandez,L., Carey,A.H., Schaldach,M.A.,
            Smithwick,M.J., Rus,K., Marshall Graves,J.A., Stewart,C.L. and
            Nicholls,R.D.
  TITLE     The ancient source of a distinct gene family encoding proteins
            featuring RING and C(3)H zinc-finger motifs with abundant
            expression in developing brain and nervous system
  JOURNAL   Genomics 66 (1), 76-86 (2000)
   PUBMED   10843807
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153624.20.
            
            On Aug 23, 2022 this sequence version replaced NM_018810.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077060.1, SRR13861889.27057.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-355               AC153624.20        165335-165689       c
            356-484             AC153624.20        155324-155452       c
            485-714             AC153624.20        150967-151196       c
            715-941             AC153624.20        149884-150110       c
            942-1156            AC153624.20        146446-146660       c
            1157-1267           AC153624.20        145631-145741       c
            1268-1406           AC153624.20        145034-145172       c
            1407-3037           AC153624.20        143040-144670       c
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 18.18 cM"
     gene            1..3037
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /note="makorin, ring finger protein, 1"
                     /db_xref="GeneID:54484"
                     /db_xref="MGI:MGI:1859353"
     exon            1..355
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     CDS             171..1616
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase makorin-1; RING-type E3 ubiquitin
                     transferase makorin-1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase makorin-1 isoform 1"
                     /protein_id="NP_061280.2"
                     /db_xref="CCDS:CCDS20022.1"
                     /db_xref="GeneID:54484"
                     /db_xref="MGI:MGI:1859353"
                     /translation="MAEAAAPGTTATTSGAGAAAAAVAAASLTSIPTVAAPSPGAGGG
                     GGGSDGSGGGWTKQVTCRYFMHGVCKEGDNCRYSHDLSDSPYGVVCKYFQRGYCVYGD
                     RCRYEHSKPLKQEEVTATDLSAKPSLAASSSLSSGVGSLAEMNSGEAESRNPSFPTVG
                     AGSEDWVNAIEFVPGQPYCGRTAPSCTEVPPQGSVTKEESEKEPTTVETKKQLCPYAA
                     VGECRYGENCVYLHGDSCDMCGLQVLHPVDAAQRSQHIKSCIEAHEKDMELSFAVQRS
                     KDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCPEC
                     RITSNFVIPSENWVEEKEEKQKLIQKYKEAMSNKACRYFDEGRGSCPFGGNCFYKHAY
                     PDGRREEPQRQKVGTSSRYRAQRRSHFWELIEERENNPFDNDEEEVVTFELGEMLLML
                     LAAGGDDELTDSEDEWDLFHDELEDFYDLDL"
     exon            356..484
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            485..714
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            715..941
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1156
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1267
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1406
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..3037
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1810..1815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /note="hexamer: AATAAA"
     polyA_site      1843
                     /gene="Mkrn1"
                     /gene_synonym="RFP"
                     /note="major polyA site"
ORIGIN      
        1 gagacctgag cgctcggaga cgcggtgaca cggcgcggga ctggcgcaga gctctcgggc
       61 tcctcagcct ccctcccctc ccctcccttc gctgtcttct ccttctcagc cggaccggaa
      121 ctatgtgatc ccggaagttc cggggccatt gctgtgtggg ataaacagta atggcggagg
      181 ctgcggctcc cggaacaaca gccacaacat caggagcagg agcggcagcg gcggcggtgg
      241 cagcggcctc cctcacctct atccccacag tcgctgcccc gtccccgggg gcgggaggag
      301 ggggcggcgg cagcgacggc agcggcggcg gctggacgaa acaggtcacc tgcagatact
      361 ttatgcatgg ggtttgtaag gaaggcgata actgtcgata ctctcatgac ctctctgaca
      421 gtccatacgg cgtagtgtgc aagtattttc aaagagggta ctgtgtttat ggagaccgct
      481 gcagatacga acacagcaag ccactgaaac aggaagaagt gactgctact gatctgagtg
      541 caaaaccatc cctcgctgct tcctcaagtc tctcttctgg tgttggatca cttgctgaaa
      601 tgaattcagg cgaagctgag tcacgaaacc caagctttcc aactgtagga gcaggttcag
      661 aggactgggt gaatgccatt gagtttgtcc ctgggcagcc ctactgtggc cgtactgccc
      721 cttcctgcac tgaagtaccc ccgcagggtt cagtgaccaa ggaagagtca gagaaggagc
      781 caactacggt ggagacaaag aagcagcttt gcccctatgc tgcagtggga gagtgtcgct
      841 atggggaaaa ctgtgtgtat ctccacggag actcgtgtga catgtgtggc ctgcaggtcc
      901 tacacccagt ggatgctgcc cagaggtcac agcacataaa atcttgcatt gaggcccatg
      961 agaaggacat ggagctctct tttgctgttc agcgcagcaa ggacatggtg tgtgggatct
     1021 gcatggaggt ggtctatgag aaagccaacc ccagcgagcg tcgctttggg atcctctcca
     1081 actgcaacca cacctactgt ctcaagtgta ttcgcaagtg gagaagtgct aaacaatttg
     1141 agagcaagat cataaagtcc tgccccgaat gccggatcac atctaacttt gtcattccaa
     1201 gtgagaactg ggtggaggag aaagaagaga agcagaaact cattcagaaa tacaaggagg
     1261 ccatgagcaa caaggcgtgc aggtattttg atgaaggacg tgggagctgc ccatttggag
     1321 ggaactgttt ttacaagcat gcgtaccctg atggccgtag agaggagcca cagagacaga
     1381 aagtgggaac atcaagcaga taccgggccc aacgaaggag ccacttctgg gagctcattg
     1441 aggagagaga gaacaacccc tttgacaacg acgaagagga ggttgtcacc tttgagctgg
     1501 gcgagatgtt gcttatgctt ttggctgcag gtggggacga cgagctgaca gactctgagg
     1561 acgagtggga cttgtttcac gatgagctgg aggactttta tgacttggat ctatagcagc
     1621 gttgcgtggc gcgggcactg gtctgcggag cctcagacag tagctgtccc ctgtgctgtg
     1681 tggcagtgcg tgtggctctc ctaggcaggc ctctcaaact ccaggtgctg tgatcacttc
     1741 ccagggcctg ttcctctacc ctcacctccc ccaagagtgt gttgttttct ctgtttcaaa
     1801 agttacaaaa ataaatctta aagttagttt tttgtaacat gaatttaact gtcagacagt
     1861 tagtgtaggt ttgttgcgcc atctgtcctc agccaggttg tgttcatgga ctttgcacac
     1921 tcattctgag acccctttga ggttcaaagg aaaaagcact gattgctttg gggtccaagg
     1981 ataggggcaa ttgggaaaaa agggacctaa gtcgctctct gcgctagatg tgggagggct
     2041 ccccgaggtt tctgggagat ctgcaccttc atggtttaga gctgttccct gccctgtaat
     2101 cccctacatg tgcttgtcct gtgggtttgg gttcttgttc agttgcacgc aagaaagact
     2161 actgggtaac cagaaccagg tgcaaatgtg ttgaggttaa cctgagaaaa atgtaaaaac
     2221 acagggagga tgcagtggca ttccatcagc gcagagctgg cagctaactc ccaagggtcg
     2281 atggacttgg tgggcgagca cgagcgagca tgtgagcagc ttccttccca tctgtgcata
     2341 ggtgcagtat tcttagcttt agtgggcaga tgtgggttag tggtttcagc cacatgtgtc
     2401 ggtagatctt aaaggaccaa gcagtatgtc agtagttgtt aacgtagcag tagctgtctg
     2461 tctgtatgct agagaaacga gatcagctgt agaggttaaa cgacctgctt gtccccctct
     2521 cagtacaagc tgggtcttgg acacactgat gttttgaatg ttttacagtc tagccttttc
     2581 agtcaggcac tttttggaaa aaactttgtc ctcatttgag ggggcctgtt gtgaggttct
     2641 gtgcttgttt ttgtgggtat ctgcctcatt ccatccctga gctttgacag gaagacaggt
     2701 ggattccaaa ctgaggtgct ggttgtacta gaggtatctg tcggtttgca gaagtaatga
     2761 acccttcccc accgaagaaa agggagggtg cggccaccca cgaggcaagc aaaagttaag
     2821 ttgctaaaat tccttggcgg gaatttcacc tttgcatttt gttgctctct ttcctgaaat
     2881 aacgtgggag atgctctgat ctgtcctctg ctttgcttcc gcggaactca cccagtctta
     2941 aaaacacatc atcattgcag aggcctctgt attttgcagc tgcccttttg taagaagcac
     3001 ttttcccaaa taaaacgatt taaaaaaaaa aaaaaaa
//
