LOCUS       NM_030218               2427 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus mitotic spindle positioning (Misp), mRNA.
ACCESSION   NM_030218
VERSION     NM_030218.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Hiura,K., Maruyama,T., Watanabe,M., Nakano,K., Okamura,T.,
            Sasaki,H. and Sasaki,N.
  TITLE     Mitotic spindle positioning protein (MISP) deficiency exacerbates
            dextran sulfate sodium (DSS)-induced colitis in mice
  JOURNAL   J Vet Med Sci 85 (2), 167-174 (2023)
   PUBMED   36596561
  REMARK    GeneRIF: Mitotic spindle positioning protein (MISP) deficiency
            exacerbates dextran sulfate sodium (DSS)-induced colitis in mice.
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124407.4.
            
            On Mar 21, 2007 this sequence version replaced NM_030218.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK020266.1, BC013508.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-73                AC124407.4         138156-138228
            74-1812             AC124407.4         142831-144569
            1813-1946           AC124407.4         145013-145146
            1947-1985           AC124407.4         146528-146566
            1986-2427           AC124407.4         147146-147587
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2427
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /note="mitotic spindle positioning"
                     /db_xref="GeneID:78906"
                     /db_xref="MGI:MGI:1926156"
     exon            1..73
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            74..1812
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..2075
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /note="mitotic spindle positioning protein"
                     /codon_start=1
                     /product="mitotic interactor and substrate of PLK1"
                     /protein_id="NP_084494.1"
                     /db_xref="CCDS:CCDS23992.1"
                     /db_xref="GeneID:78906"
                     /db_xref="MGI:MGI:1926156"
                     /translation="MDRVTRYPIFSNPRSARVTSLVLDEDTSYTVELVGVGPEAGWSQ
                     GDLQAWSTEYQTRPDVKRTNVSSNRRVFLGQQSPRSLYLEDEDEEIKSYHLDDSSDTL
                     CRQRRELEAERWAVIQSQAVRKGGTVATLQAASDHGDLRIARQPQSTVTEEILVDTEQ
                     IDFLAARQQFLSLEKANTNPVPCGLTARETFPRTPPGINQAPKASTGPHLANGYTTAV
                     TSPMKEVTLEKSFHVSPARSIHIVNDPGSQTQAESPETPKETPIEREIRLAQEREAEL
                     REQRGLGRAAGHQELVQIPSRPLLSKMSLTETPPRRDRGRPSLYVQRDMVQETQREED
                     HRREGLQVGRASTPDWPSQDPQPGLQRSLSSDCILSPDARATDPAPEARKVNRIPLDA
                     YQPYLGPGTPKLEFSAFGVYSKPSGVSTEDTKATVFQKATESPRHVSESSGRSLSSKQ
                     EWSKPPGKATNVGVVRLGNFHLRPLRFKVPDVPQGTETPHTWGWEVAGGPILRLQKSQ
                     SSDLLEREMESVLRREREVAEERRNAFFPEVFSPVPAEDESHEQDSRSSSRASGITGS
                     YSVSESPLFTPVHLNSGLVWKVEAPEDSAPPGQKTRKEMWYAGINPSDSVNSEVLGAT
                     RVKRHKNLLAERWEAHIYASEDEN"
     exon            1813..1946
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..1985
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2427
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2406..2411
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2427
                     /gene="Misp"
                     /gene_synonym="9130017N09Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagaaacttc tcccctgcgg ctgctgcagc cctcactggg cattttcgcc aggactctgc
       61 ggcacctggt cagtgagcct ggaggcctcc aacccacaga aggaaggtca cacacaagac
      121 cctttgagat ggatcgagtg accagatacc ccatcttcag caacccccgc tcagcacgtg
      181 tcaccagctt ggtactggat gaagatacca gctacaccgt tgagctagtg ggcgtggggc
      241 ctgaggctgg ttggagccag ggtgatctgc aggcctggtc cactgagtac cagaccagac
      301 cagatgtaaa gagaacaaac gtgtcttcca acagacgcgt cttccttggc cagcagtccc
      361 cgaggtcact gtacttggaa gatgaggatg aggagataaa gtcttatcac ctggacgaca
      421 gcagtgacac cctctgcagg cagcggaggg agctggaggc tgagcgctgg gcagtcatcc
      481 agagccaggc agtcaggaaa ggtggcacag tggccacact ccaggctgca tcagaccatg
      541 gggacctcag gattgccaga caacctcagt ccacagtcac agaggagatc ttggtggaca
      601 cggagcaaat tgatttcctg gctgctcgac agcaattcct gagcttagaa aaggcaaaca
      661 caaacccggt cccctgcggc ctcacagcca gggagacctt cccacgaacc ccaccaggca
      721 tcaaccaagc ccccaaggct tccactgggc cccacctagc caatggctat accactgccg
      781 tcacgtcacc aatgaaggaa gtgaccctgg agaagtcttt ccatgtttcc ccagctagat
      841 ccatccatat agtcaatgat cctggcagtc agacccaggc agaatcccct gagaccccca
      901 aagagacgcc catcgagaga gagatcaggc tggcccagga gcgagaggca gagctgaggg
      961 agcagagagg gcttgggcga gctgccgggc atcaagagct ggtacagatc cccagcaggc
     1021 cactgctcag caagatgagc ttgacagaga cacctccacg aagagacagg ggccgcccat
     1081 ccctctatgt gcaaagggac atggtgcaag agacacagcg ggaagaagat catcgacgag
     1141 aggggctgca ggtgggcagg gcatccacac ctgactggcc ttcccaggac cctcagcctg
     1201 ggctccagag aagcctgagc tccgattgca tcctcagccc agatgcccga gccacagacc
     1261 cggctccaga ggcaaggaaa gtcaaccgga tcccactgga tgcctaccag ccatatctag
     1321 gccctgggac ccctaaacta gaattctcag cctttggagt atacagcaag ccaagtggtg
     1381 tctccaccga agacaccaaa gccacagtct tccagaaggc cacggagtct ccaaggcatg
     1441 tctcagaatc ctccggcaga tcgctgagct caaagcaaga gtggtccaag cctcctggaa
     1501 aagctaccaa cgtgggtgtg gtgagattag ggaatttcca cctgcgtcca ctgcgattca
     1561 aggtcccaga tgtcccccag gggactgaga ccccccatac ctggggctgg gaagtggctg
     1621 gaggccccat attgaggcta caaaagtcac agtcatctga cctgctggag agggaaatgg
     1681 agagtgtcct gcggagggag cgggaggtgg ctgaagagcg gaggaatgct ttcttcccag
     1741 aggtgttctc cccagtgcca gccgaggatg agagccatga gcaagactcc cggagttcct
     1801 cccgggcatc tggcatcact ggcagctatt cagtgtcaga atcaccactc ttcacccccg
     1861 tccacctgaa ttcggggctg gtgtggaagg tggaggcccc tgaggacagt gctcccccag
     1921 ggcagaagac taggaaggag atgtggtatg caggtatcaa cccctcagac agtgtcaact
     1981 cagaggtcct gggagccaca agggtaaagc ggcacaagaa tctcttggcg gaacgctggg
     2041 aagcacacat ctatgccagc gaggatgaaa actgagactg gaagcagggg gccattctcc
     2101 tgcctcactg cagtcctgtg gaggccgcta gacctgcccc cagtctctgc tgtccctatt
     2161 taaacataca gcccaggctg atgtttgtgg cctgcagttg atttggtttt ccctggggtg
     2221 ggcgagtgat tcgtgcttag gggtggttct cagctaaggc tgaaatgata atccaccgat
     2281 ccccctcaca tccctcagcc ctccgcacag cccagagctt ggatctgtca ctgcagaaag
     2341 aaggctctgt ttggggacct gccattccac tcttgccaac cccaaacgta tgcctgttgc
     2401 tatgaaataa acctgtcctg aactgcc
//
