LOCUS       NM_025993               2993 bp    mRNA    linear   ROD 08-JUN-2024
DEFINITION  Mus musculus MIS12 kinetochore complex component (Mis12), mRNA.
ACCESSION   NM_025993
VERSION     NM_025993.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Bai,G.Y., Choe,M.H., Kim,J.S. and Oh,J.S.
  TITLE     Mis12 controls cyclin B1 stabilization via Cdc14B-mediated
            APC/CCdh1 regulation during meiotic G2/M transition in mouse
            oocytes
  JOURNAL   Development 147 (8) (2020)
   PUBMED   32341029
  REMARK    GeneRIF: Mis12 controls cyclin B1 stabilization via Cdc14B-mediated
            APC/C(Cdh1) regulation during meiotic G2/M transition in mouse
            oocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Varma,D. and Salmon,E.D.
  TITLE     The KMN protein network--chief conductors of the kinetochore
            orchestra
  JOURNAL   J Cell Sci 125 (Pt 24), 5927-5936 (2012)
   PUBMED   23418356
  REMARK    Review article
REFERENCE   3  (bases 1 to 2993)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2993)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL596136.5.
            
            On Jan 23, 2019 this sequence version replaced NM_025993.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026790.1, AK010995.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-579               AL596136.5         178828-179406
            580-741             AL596136.5         183767-183928
            742-2993            AL596136.5         184327-186578
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 43.21 cM"
     gene            1..2993
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /note="MIS12 kinetochore complex component"
                     /db_xref="GeneID:67139"
                     /db_xref="MGI:MGI:1914389"
     exon            1..579
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /inference="alignment:Splign:2.1.0"
     exon            580..741
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /inference="alignment:Splign:2.1.0"
     exon            742..2993
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /inference="alignment:Splign:2.1.0"
     CDS             775..1395
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /note="MIS12 homolog"
                     /codon_start=1
                     /product="protein MIS12 homolog"
                     /protein_id="NP_080269.1"
                     /db_xref="CCDS:CCDS24973.1"
                     /db_xref="GeneID:67139"
                     /db_xref="MGI:MGI:1914389"
                     /translation="MSVDPMAYEAQFFGFTPQTCLLRIYVAFQDHLFEVMQAVEQVIL
                     KKLEDIPNCEITPVQTRKCTEKFLCFMKGRFDNLFGKMEQLILQSILCIPPNILLPED
                     KCQETNPFSEEKLELLQQEIKELQEKYKVELCTEQALLAELEEQKTVKAKLRETLTFF
                     DELENIGRYQGTSNFRESLASLVQSCRKLQSIRDNVEKESRRLETQ"
     regulatory      2988..2993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /note="hexamer: AATAAA"
     polyA_site      2993
                     /gene="Mis12"
                     /gene_synonym="2510025F08Rik; Misc12"
                     /note="major polyA site"
ORIGIN      
        1 agtcgattcg gaggagccgg tgttaccgcc ttcttccagc cgctgagtgt agcttgaagg
       61 aagtggcggg aagccggtgt ggggcggaat ccatcactcc cgggatcgct tcggtagggg
      121 ctgtgtgctc tccgaggagg agcggaaagg gcgggaccga ggagtggcgt cggggcggcg
      181 gagcgttctg tagggaatcc ctccggtctg gccggcctgg gtacggagac ctagagttcc
      241 cggcatgcat cgcggccgcg gggtagtacg ggagctggag ctgaaggaag cgcttcccgt
      301 tctgcggttt ctgggccttg tctgtggcgg tcgtaggctc tgaagtccat tacattcgtc
      361 gagacggtgt gaggacctct catgtttatc ttcagccgca ggaactctaa tctagcagag
      421 aagagacggt agatgaaggg aaagcggagg cggttcttca gggatgtgta ttcgaactgg
      481 ctctggaatc tgcgcctact gcatgcttac ttgaaggaaa gaaaatagaa ttagattggg
      541 ctggagcaaa gactatcctg accgtggcag acgtgttcag attccagcat tctggtggta
      601 gaagcagaag gatcaaaaat tcaagaccat cctaagctac attccaagtt tgaggccatc
      661 ttcagctaca tgagaccctg gcacaaatga aagagtttga ggccaaccca taggcgacac
      721 agcaagaaat tgtcttcaga gattttcaca gctgagagtg caaaataaag caggatgtct
      781 gtggatccga tggcctatga ggcccagttc tttggcttca caccacagac ttgcctgctg
      841 aggatctacg tagcatttca agaccacctg tttgaagtga tgcaggctgt tgaacaggtt
      901 atcctaaaga agctggagga cattccaaac tgtgagatca cccctgtcca gactcggaaa
      961 tgcacagaga agtttctttg cttcatgaaa ggacgcttcg ataacctttt tggcaaaatg
     1021 gagcagctga ttttgcagtc gattttgtgt attcctccaa acatcctgct tcctgaagac
     1081 aagtgtcagg agacgaatcc tttcagtgaa gagaaactcg agcttctcca acaggaaatc
     1141 aaagagttac aggagaaata caaggttgag ttgtgcactg agcaggccct tcttgcagaa
     1201 ttagaggagc agaaaactgt taaggccaaa ctcagagaga ccttgacttt ctttgatgag
     1261 cttgaaaaca tcggcagata tcaaggaact agtaacttta gggagagttt ggcatccctg
     1321 gtccagagct gcagaaaact tcagagcatt agagacaatg tagaaaaaga aagcaggaga
     1381 ctggaaacac agtgatttct cagtaatgaa aagagagcat agaaatagat agcttttctt
     1441 tgtttattta ctcttatgtt taggtctttt ggggccacgc tcccctcctg tcctgggtta
     1501 cacaaagcaa atcagcagac cttctaaatc gcaggctgca gtggagacct cagaagcaga
     1561 gcttgtgtcc atttgctttt ctctctcaaa cttggctcac atctggtaaa gcctccagtt
     1621 cttttgggat tgcactcatt ttgctgttag gtatgtttag tatgatttca ggagggaata
     1681 gaatcctcat ggaactggat gtttctgctc atttttgctg tataaagcag gtctgtattc
     1741 attgagtata accatgaggt acatcaggga ctaatagagc cgggctgagg acatagctca
     1801 gtggtagagt atgctcaaca tacttaggat ttaacatgga gagaagcaga aagaggagtg
     1861 ggaaggagga gaaggaaatc aaagggcagc agggcacagt agaggggatc agggtatggg
     1921 atgacatata gtgcaatata ttaaaggggc atgtcccatt gataatgtaa gattcaagtc
     1981 aagcttagaa ggtgttagcc tctcacctaa gagatcactc taggcaaaga aaatagcaga
     2041 aaacccataa ggtagaacat atggaattca cacagctgga acagatatgg ccaatttagg
     2101 aaatgggata cgcctgtcac attccagtct actttttgta agtctgatga gatgggtcgg
     2161 caggtaaaat acttgtaata caagcctggt gacctgagta ggatccccaa aacctactta
     2221 aagatggaaa gaattgactc cacaaagttg tcttaaggta cactgtggca tgtacactct
     2281 catcccccac attgtgtgta cacacacagt ctctcttaag agacaagcaa aacctctgtg
     2341 tatgtgtgag tatgtgctat taaaaactaa ctcctagctg gatttcatgc cgttgatccc
     2401 agtactcagg aggcagaggc aggtggacct cttgtgaatt tagcctggtt tacataaaga
     2461 gttcagggca gccaggacta aagagacccc atctcagaaa acaaaaaacc caaatgtgat
     2521 tgatggcact tgttagattg gtaaagcaga ctttattcag gacccttaag ttagatactg
     2581 ggaccattgc agaaggattt tgcattgaga gattagattc tgaatataag aagtaaaaag
     2641 atgtttggga atgtgcaact aagggacaga atgggacttg ttgggacaaa gcaagtatca
     2701 ttggatggaa aattactaaa agggtcggta taaggggttt gaatgtatcc cataaaattc
     2761 atatgttaaa actcttgact tcttagtggt acactgtaga ggtttatact ttgattaatg
     2821 tcagtactgt gggaacagga gtgttcctta gggaatagat tcccaataaa ggatggactc
     2881 aggtccttct gctctggcat gatctcaccc tgtaacacct gagacagcag gaaggcatgt
     2941 aactttggat tctatacata caataaataa tctctatacc tgtaataaat aaa
//
