LOCUS       NP_081712                711 aa            linear   ROD 03-JUN-2025
DEFINITION  mitochondrial intermediate peptidase precursor [Mus musculus].
ACCESSION   NP_081712 XP_919508 XP_928329
VERSION     NP_081712.2
DBSOURCE    REFSEQ: accession NM_027436.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 711)
  AUTHORS   Nozaki,Y., Kobayashi,M., Fukuoh,T., Ishimatsu,M., Narita,T.,
            Taki,K., Hirao,Y., Ayabe,S., Yokoyama,M., Otani,Y., Mizunoe,Y.,
            Matsumoto,M., Ohno,N., Kaifu,T., Okazaki,S., Goitsuka,R.,
            Nakagawa,Y., Shimano,H., Iwakura,Y. and Higami,Y.
  TITLE     Mipep deficiency in adipocytes impairs mitochondrial protein
            maturation and leads to systemic inflammation and metabolic
            dysfunctions
  JOURNAL   Sci Rep 15 (1), 12839 (2025)
   PUBMED   40229443
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 711)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 711)
  AUTHORS   Kobayashi,M., Takeda,K., Narita,T., Nagai,K., Okita,N., Sudo,Y.,
            Miura,Y., Tsumoto,H., Nakagawa,Y., Shimano,H. and Higami,Y.
  TITLE     Mitochondrial intermediate peptidase is a novel regulator of
            sirtuin-3 activation by caloric restriction
  JOURNAL   FEBS Lett 591 (24), 4067-4073 (2017)
   PUBMED   29151261
  REMARK    GeneRIF: MIPEP may contribute to the maintenance of mitochondrial
            quality during caloric restriction (CR) via activation of SIRT3.
            This novel mechanism of SIRT3 activation through MIPEP facilitates
            the elucidation of additional molecular pathways of CR.
REFERENCE   4  (residues 1 to 711)
  AUTHORS   Gomez-Del Arco,P., Perdiguero,E., Yunes-Leites,P.S., Acin-Perez,R.,
            Zeini,M., Garcia-Gomez,A., Sreenivasan,K., Jimenez-Alcazar,M.,
            Segales,J., Lopez-Maderuelo,D., Ornes,B., Jimenez-Borreguero,L.J.,
            D'Amato,G., Enshell-Seijffers,D., Morgan,B., Georgopoulos,K.,
            Islam,A.B., Braun,T., de la Pompa,J.L., Kim,J., Enriquez,J.A.,
            Ballestar,E., Munoz-Canoves,P. and Redondo,J.M.
  TITLE     The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle
            Identity and Metabolic Homeostasis
  JOURNAL   Cell Metab 23 (5), 881-892 (2016)
   PUBMED   27166947
REFERENCE   5  (residues 1 to 711)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CA460475.1, AK017491.1, AI606287.1 and BE995975.1.
            
            On Nov 15, 2007 this sequence version replaced NP_081712.1.
            
            Transcript Variant: This variant (1) encodes the functional
            protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC110358.1, AK038479.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 31.95 cM"
     Protein         1..711
                     /product="mitochondrial intermediate peptidase precursor"
                     /EC_number="3.4.24.59"
                     /calculated_mol_wt=77450
     transit_peptide 1..33
                     /note="Mitochondrion. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (A6H611.1)"
                     /calculated_mol_wt=3421
     mat_peptide     34..711
                     /product="Mitochondrial intermediate peptidase.
                     /id=PRO_0000319045"
                     /note="propagated from UniProtKB/Swiss-Prot (A6H611.1)"
                     /calculated_mol_wt=77450
     Region          62..695
                     /region_name="M3A_MIP"
                     /note="Peptidase M3 mitochondrial intermediate peptidase
                     (MIP); cd06457"
                     /db_xref="CDD:341052"
     Site            124
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q99797; propagated from
                     UniProtKB/Swiss-Prot (A6H611.1)"
     Site            order(444..446,493..494,497,522,525,574,621..622,626..627,
                     629..630,633)
                     /site_type="active"
                     /db_xref="CDD:341052"
     CDS             1..711
                     /gene="Mipep"
                     /gene_synonym="5730405E07Rik; MIP"
                     /coded_by="NM_027436.3:123..2258"
                     /db_xref="CCDS:CCDS49517.1"
                     /db_xref="GeneID:70478"
                     /db_xref="MGI:MGI:1917728"
ORIGIN      
        1 mllaagarya rrlcgrgaaa alqgrtgrsc ardvstswsp vgaafnvkpq shlwnllger
       61 rglfgvpels tpegfqvaqe ealkktewlv eracstppgp qtvlifdels dclcrvadla
      121 dfvkighpdp afreaaqeac rsigtmvekl ntnvelyqsl qrllgdkklm esldaetrrv
      181 aelfmfdfei sgihldeekr rravdlnvki ldlsnaflmr tnfpnkirks llpehiqhhf
      241 ardgshliid glhaeasddl vreaaykifl ypnadqlkcl eellssrdll aklvgystfs
      301 hralqgtiaq tpetvmqfle klseklsert rkdfkmmqgm ktklnpqnsk lmpwdppyys
      361 gviraeryni epslycpfls lgacmeglnv lfnkllgitl yaeqtfkgev wcndirklav
      421 vhesegllgy iycdffqran kpqqdchfti rggrlkedgs yqlpvvvlml nlphasrdfp
      481 tlltpgmmen lfhemghamh smlgrtryqh vtgtrcptdf aevpsilmey fsndyrvvsq
      541 fakhyqtgqp lpkamvsrlc eskkvctaae mqlqvfyaal dqiyhgqhpl kksttdilme
      601 tqeqfyglpy vpdtawqlrf shlvgygaky ysylmsrava smiwkecflq dpfnraager
      661 yrremlahgg gkepmlmiqg mlqkcpsidd fvdalvsdmn ldfetfflds k
//
