LOCUS       NM_001045959            4987 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1),
            transcript variant 3, mRNA.
ACCESSION   NM_001045959
VERSION     NM_001045959.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Zhan,K., Zhu,K., Gu,B., Yao,S., Fu,F., Zeng,H., Tian,K., Ji,W.,
            Jin,H., Tong,P., Wu,C., Yue,M. and Ruan,H.
  TITLE     MINK1 deficiency stimulates nucleus pulposus cell pyroptosis and
            exacerbates intervertebral disc degeneration
  JOURNAL   Int Immunopharmacol 134, 112202 (2024)
   PUBMED   38723371
  REMARK    GeneRIF: MINK1 deficiency stimulates nucleus pulposus cell
            pyroptosis and exacerbates intervertebral disc degeneration.
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4987)
  AUTHORS   Yu,D., Hu,J., Sheng,Z., Fu,G., Wang,Y., Chen,Y., Pan,Z., Zhang,X.,
            Wu,Y., Sun,H., Dai,J., Lu,L. and Ouyang,H.
  TITLE     Dual roles of misshapen/NIK-related kinase (MINK1) in
            osteoarthritis subtypes through the activation of TGFbeta signaling
  JOURNAL   Osteoarthritis Cartilage 28 (1), 112-121 (2020)
   PUBMED   31647983
  REMARK    GeneRIF: Dual roles of misshapen/NIK-related kinase (MINK1) in
            osteoarthritis subtypes through the activation of TGFbeta
            signaling.
REFERENCE   4  (bases 1 to 4987)
  AUTHORS   Fu,G., Xu,Q., Qiu,Y., Jin,X., Xu,T., Dong,S., Wang,J., Ke,Y.,
            Hu,H., Cao,X., Wang,D., Cantor,H., Gao,X. and Lu,L.
  TITLE     Suppression of Th17 cell differentiation by misshapen/NIK-related
            kinase MINK1
  JOURNAL   J Exp Med 214 (5), 1453-1469 (2017)
   PUBMED   28400474
  REMARK    GeneRIF: a critical regulator of Th17 cell differentiation
REFERENCE   5  (bases 1 to 4987)
  AUTHORS   Diril,M.K., Bisteau,X., Kitagawa,M., Caldez,M.J., Wee,S.,
            Gunaratne,J., Lee,S.H. and Kaldis,P.
  TITLE     Loss of the Greatwall Kinase Weakens the Spindle Assembly
            Checkpoint
  JOURNAL   PLoS Genet 12 (9), e1006310 (2016)
   PUBMED   27631493
  REMARK    GeneRIF: using in vitro dephosphorylation assays, we demonstrate
            that Mastl promotes persistent MPS1 phosphorylation by inhibiting
            PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1
            kinase activity. Our findings establish a key regulatory function
            of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing
            premature SAC silencing
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4987)
  AUTHORS   Hu,Y., Leo,C., Yu,S., Huang,B.C., Wang,H., Shen,M., Luo,Y.,
            Daniel-Issakani,S., Payan,D.G. and Xu,X.
  TITLE     Identification and functional characterization of a novel human
            misshapen/Nck interacting kinase-related kinase, hMINK beta
  JOURNAL   J Biol Chem 279 (52), 54387-54397 (2004)
   PUBMED   15469942
  REMARK    Erratum:[J Biol Chem. 2005 Feb 11;280(6):5128]
REFERENCE   7  (bases 1 to 4987)
  AUTHORS   Dan,I., Watanabe,N.M., Kajikawa,E., Ishida,T., Pandey,A. and
            Kusumi,A.
  TITLE     Overlapping of MINK and CHRNE gene loci in the course of mammalian
            evolution
  JOURNAL   Nucleic Acids Res 30 (13), 2906-2910 (2002)
   PUBMED   12087176
REFERENCE   8  (bases 1 to 4987)
  AUTHORS   Dan,I., Watanabe,N.M., Kobayashi,T., Yamashita-Suzuki,K.,
            Fukagaya,Y., Kajikawa,E., Kimura,W.K., Nakashima,T.M.,
            Matsumoto,K., Ninomiya-Tsuji,J. and Kusumi,A.
  TITLE     Molecular cloning of MINK, a novel member of mammalian GCK family
            kinases, which is up-regulated during postnatal mouse cerebral
            development
  JOURNAL   FEBS Lett 469 (1), 19-23 (2000)
   PUBMED   10708748
REFERENCE   9  (bases 1 to 4987)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
REFERENCE   10 (bases 1 to 4987)
  AUTHORS   Osada,S., Izawa,M., Saito,R., Mizuno,K., Suzuki,A., Hirai,S. and
            Ohno,S.
  TITLE     YSK1, a novel mammalian protein kinase structurally related to
            Ste20 and SPS1, but is not involved in the known MAPK pathways
  JOURNAL   Oncogene 14 (17), 2047-2057 (1997)
   PUBMED   9160885
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL592547.9.
            
            On Nov 18, 2022 this sequence version replaced NM_001045959.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.3292524.1,
                                           SRR13422603.359342.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-259               AL592547.9         71511-71769         c
            260-325             AL592547.9         37404-37469         c
            326-382             AL592547.9         35739-35795         c
            383-508             AL592547.9         35532-35657         c
            509-619             AL592547.9         32913-33023         c
            620-710             AL592547.9         32580-32670         c
            711-841             AL592547.9         31824-31954         c
            842-896             AL592547.9         31318-31372         c
            897-975             AL592547.9         31089-31167         c
            976-1151            AL592547.9         30694-30869         c
            1152-1221           AL592547.9         30384-30453         c
            1222-1432           AL592547.9         29432-29642         c
            1433-1549           AL592547.9         28473-28589         c
            1550-1774           AL592547.9         27493-27717         c
            1775-1948           AL592547.9         27122-27295         c
            1949-2212           AL592547.9         26617-26880         c
            2213-2298           AL592547.9         26211-26296         c
            2299-2409           AL592547.9         25938-26048         c
            2410-2528           AL592547.9         25679-25797         c
            2529-2617           AL592547.9         25383-25471         c
            2618-2781           AL592547.9         24920-25083         c
            2782-2887           AL592547.9         24454-24559         c
            2888-3025           AL592547.9         24234-24371         c
            3026-3134           AL592547.9         23928-24036         c
            3135-3302           AL592547.9         23160-23327         c
            3303-3446           AL592547.9         22908-23051         c
            3447-3581           AL592547.9         22266-22400         c
            3582-3682           AL592547.9         22056-22156         c
            3683-3832           AL592547.9         21822-21971         c
            3833-3992           AL592547.9         21571-21730         c
            3993-4132           AL592547.9         21343-21482         c
            4133-4987           AL592547.9         20154-21008         c
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 43.0 cM"
     gene            1..4987
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /note="misshapen-like kinase 1 (zebrafish)"
                     /db_xref="GeneID:50932"
                     /db_xref="MGI:MGI:1355329"
     exon            1..259
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..4216
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /EC_number="2.7.11.1"
                     /note="isoform 3 is encoded by transcript variant 3; MEKKK
                     6; MEK kinase kinase 6; GCK family kinase MiNK;
                     misshapen/NIK-related kinase; MAPK/ERK kinase kinase
                     kinase 6; Yeast Sps1/Ste20-related kinase 2;
                     Misshapen/NIKs-related kinase"
                     /codon_start=1
                     /product="misshapen-like kinase 1 isoform 3"
                     /protein_id="NP_001039424.1"
                     /db_xref="CCDS:CCDS36205.1"
                     /db_xref="GeneID:50932"
                     /db_xref="MGI:MGI:1355329"
                     /translation="MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRH
                     VKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLW
                     LVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVL
                     LTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWS
                     LGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLS
                     RPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHG
                     EEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLH
                     QRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQE
                     YKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYH
                     YGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQ
                     TPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPA
                     AVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATAL
                     NTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDL
                     RRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHR
                     SRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDT
                     PGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNL
                     PDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGD
                     TIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCA
                     ALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLR
                     VYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVY
                     AWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYD
                     IYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTS
                     VAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGG
                     SSQVYFMTLNRNCIMNW"
     exon            260..325
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            326..382
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            383..508
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            509..619
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            620..710
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            711..841
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            842..896
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            897..975
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1151
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1221
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1432
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1549
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1774
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1948
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2212
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2213..2298
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2409
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2528
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2617
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2781
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2782..2887
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2888..3025
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3026..3134
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3135..3302
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3303..3446
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3447..3581
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3582..3682
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3683..3832
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3833..3992
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            3993..4132
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     exon            4133..4987
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4959..4964
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /note="hexamer: AATAAA"
     polyA_site      4987
                     /gene="Mink1"
                     /gene_synonym="B55; Map4k6; MINK; Ysk2"
                     /note="major polyA site"
ORIGIN      
        1 agggcagccg gcgactcggg ttgcaggcgc gcgggtgaga gcgcctgtca gccgctgggt
       61 cggtcctccc gctggccctc cccctccccg gccttcgggg caggcgcggt ggagcccgcc
      121 cctcgagtcc cccgatgggg gagaagcggc gacggcggca gcggagtgag cgagccggag
      181 cgtgagtggc cccgcggcgg ccatgggcga cccagccccc gcccgcagcc tggacgacat
      241 cgacctgtct gccctgcggg accctgcagg aatctttgag ctggtggagg tggttggcaa
      301 tggaacctat ggacaggtat acaaggggcg gcacgtcaag actgggcagc tggctgccat
      361 taaggtcatg gatgtcacag aggatgagga ggaagagatc aaacaggaaa tcaacatgtt
      421 aaagaagtac tctcaccatc gcaatattgc cacctactat ggggccttta tcaagaagag
      481 ccctcctggg aacgatgacc agctctggct ggtgatggag ttctgcggtg ctggttcagt
      541 gaccgacctg gtaaagaaca caaaagggaa cgcactgaag gaggattgca ttgcttacat
      601 ctgcagggag attctcaggg gtcttgccca tctccatgcc cacaaggtga tccacagaga
      661 tatcaaggga caaaatgtgc tgctgacaga gaatgctgaa gtcaagctag tggattttgg
      721 ggtgagtgct cagctggacc gcactgtggg caggcggaac actttcattg gaaccccata
      781 ctggatggct ccagaagtca ttgcctgtga cgagaacccc gatgccacct atgactacag
      841 gagtgacatt tggtctctag gaatcacagc cattgaaatg gcagagggag ccccccctct
      901 gtgtgacatg caccctatgc gggccctctt cctcatccct cggaaccctc cccccaggct
      961 caagtcaaag aaatggtcta agaagttcac tgacttcatc gacacgtgtc tcatcaagac
     1021 ttacctgagc cgcccaccca ccgaacagtt actcaaattc cccttcatcc gagaccagcc
     1081 cacggagcgg caggtccgca tccagctcaa ggaccacatc gaccgctcgc ggaagaagcg
     1141 gggtgagaaa gaggagacag agtatgagta cagcggcagt gaggaggaag acgacagcca
     1201 tggagaggaa ggcgagccaa gctccatcat gaatgtgccc ggtgagtcca cactgcgcag
     1261 agaattcctc agactccagc aggagaataa gagcaactct gaggctttaa agcagcagca
     1321 gcagctgcag caacagcagc agcgggaccc ggaggcacac atcaaacacc tgctgcacca
     1381 gcggcagcgt cgcatagagg agcagaagga ggagcggcga cgtgtggagg agcaacagcg
     1441 gcgagagcga gaacagcgta agctacaaga gaaggagcag cagcggcgat tggaagacat
     1501 gcaagcccta cgacgagagg aagagaggcg gcaagcagag cgggaacagg aatacaagcg
     1561 gaagcagctg gaggagcagc ggcagtcaga gcggctgcag agacagctgc agcaggagca
     1621 cgcctacctc aagtccctgc agcagcagca gcagcagcag cagctccaga agcagcagca
     1681 gcagcagcag cagatcctgc ctggagacag gaagcccctg tatcattacg gtcggggcat
     1741 taatcctgct gacaagccag catgggcccg cgaggtggaa gagagagcac ggatgaacaa
     1801 gcagcagaac tctcccttgg cgaaggcgaa gccaagcagt gcggggccag agccccccat
     1861 ctcccaggcc tctcctagcc ccccaggacc tctttcccag actcctccta tgcagaggcc
     1921 tgtggagccc caggaaggac cgcacaagag cctggtggca caccgggtcc cactgaagcc
     1981 atatgcagca cctgtacccc gatcccagtc cctgcaggac cagccgactc gaaacctggc
     2041 tgccttccca gcctcccacg accctgaccc tgctgctgtc cctacaccca ctgccacacc
     2101 cagtgcccga ggagctgtca tccgccagaa ttcagacccc acctctgaag ggccagggcc
     2161 tagcccaaac cctccatcct gggttcggcc tgataatgag gctccaccta aggttccaca
     2221 gaggacctct tctatcgcca ctgcccttaa caccagtggg gccggagggt cccggccagc
     2281 tcaggctgtc cgtgccagac ctcgcagtaa ctccgcctgg caaatctatc tgcagaggcg
     2341 ggcagagcgg ggcaccccca agcctcccgg gcccccagct cagccccctg gcccgcccaa
     2401 cgcctctagt aaccctgacc tcaggaggag tgaccctggc tgggagcgct cagacagtgt
     2461 cctcccggcc tcccacggcc acctccctca ggctggctcc ttggagcgga accgaaaccg
     2521 tgtgggagcc tccacaaaac tggatagctc tccagtgctc tcccctggga acaaagccaa
     2581 gcctgaagac caccgctcaa ggccaggccg gcccgcagac tttgtgttgc tcaaagagcg
     2641 gactctggat gaggccccta agcctcccaa gaaggccatg gactactcct catccagtga
     2701 ggaggtggaa agcagtgaag aggaggagga ggaaggcgat ggggagccgt cagaggggag
     2761 cagagacact cccgggggcc gcagtgatgg tgatacagac agcgtcagca ccatggtggt
     2821 tcatgatgtt gaggagatat ccgggaccca gccctcatat ggcggcggca ccatggtggt
     2881 ccagcgtact cctgaagagg aacgaagcct gctgcttgct gatagcaatg gctacacaaa
     2941 cctgcctgat gtggtccagc ccagccactc acctactgag aacagcaaag gtcaaagccc
     3001 tccaacaaag gatggaggca gtgattacca gtctcgtggg ctggtaaagg ccccaggaaa
     3061 gagctcattc accatgtttg tggatctagg gatctaccag cctggaggca gtggggacac
     3121 catccctatc acagccctag tgggtggaga aggtggtcgc cttgatcaac tgcagttcga
     3181 tgtgaggaag ggctctgtgg tcaacgtcaa tcccaccaac acccgagctc atagtgaaac
     3241 tcctgaaatt cgcaagtaca agaagcgatt caactcagag atcctatgtg cagctctctg
     3301 gggggtcaac ctcctagtgg gcacagagaa tgggctgatg ttgctggacc gaagtgggca
     3361 gggcaaggtg tatggactta ttgggcgacg acgcttccag caaatggatg tcttagaagg
     3421 gctcaacttg ctcatcacca tctcagggaa aaggaacaaa ctgcgggtat attacctgtc
     3481 ctggcttcgg aacaagatcc tacacaatga cccagaggtg gaaaagaagc aggggtggac
     3541 caccgtgggg gacatggagg gctgcggcca ctaccgtgtt gtgaaatatg aacggattaa
     3601 gttcctggtc attgccctga agaactccgt ggaggtttat gcctgggctc ccaaacccta
     3661 ccacaaattc atggccttca agtcctttgc tgacctccct caccgccctc tactggtgga
     3721 cctgacagta gaggagggac agcggctcaa ggtcatctat ggctccagtg ctggcttcca
     3781 tgctgtggat gttgattctg ggaacagcta tgacatctac atccctgtac atatccagag
     3841 ccagatcaca ccccacgcca tcatcttcct ccccaacact gatggcatgg agatgctgct
     3901 gtgctatgaa gatgagggtg tctatgtcaa cacttacggg cggatcatca aggatgtggt
     3961 gctgcagtgg ggagagatgc ccacctctgt ggcctacatc tgctccaacc agataatggg
     4021 ctggggtgag aaggccatag agatccgctc tgtggagaca ggccacctag atggggtctt
     4081 catgcacaaa cgagcccaga ggctcaagtt cctgtgtgag cgcaatgaca aggtgttttt
     4141 tgcctctgtc cgctctggag gaagcagcca agtttacttt atgactctga accgtaactg
     4201 catcatgaac tggtgacgag gcccctgggc tggggctccc cacatggacc cagctctctt
     4261 cccacagcca gactacccag gccgcccctg ccctcccacc cccttgggct tttgctttta
     4321 ctggtttgat tcactggagc ctgctgggaa cgtgacctca tatccctgaa gctttcgtga
     4381 tcacgtgacc accctttccc ccaacattct caaaactgtg cctttcccca acttgtgggg
     4441 aatgacacag cttccttccc cttaccagga attgagtggg acttgctcct ccccttttct
     4501 ccacagaaga ggagagcgct tggggcttgg accccttacc ccattgctgc tgaatgagca
     4561 gggccctgac ccctttattt gcacgccagg ggagccggct ccccccttga atgtaccaga
     4621 ctctgggggg ggggtgttca ctgggccctg ggttctggag gcggggcggt caccagccac
     4681 tccaggggca gggaccattc ctcattttct gaaagcactt taatgattcc cccccaaccc
     4741 ccagggaatg gaggagggac cccatagcca aaacattccc ccctttcctc cccactccca
     4801 ccccttctag cctctcccct tccctagaag gagagagctc agagctccca gcctttatcc
     4861 tcccttgctt gcatctgtat atagtgtgag cagcaagtag cccttctccc tcctgtatcc
     4921 tttctcaatg tagtggcctt ggatatatcc cctttgttaa taaagacaat tcaaccagct
     4981 tccacca
//
