LOCUS       NM_173759               1826 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus membrane integral NOTCH2 associated receptor 2
            (Minar2), transcript variant 3, mRNA.
ACCESSION   NM_173759 NM_001167927
VERSION     NM_173759.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Lotfollahzadeh,S., Xia,C., Amraei,R., Hua,N., Kandror,K.V.,
            Farmer,S.R., Wei,W., Costello,C.E., Chitalia,V. and Rahimi,N.
  TITLE     Inactivation of Minar2 in mice hyperactivates mTOR signaling and
            results in obesity
  JOURNAL   Mol Metab 73, 101744 (2023)
   PUBMED   37245847
  REMARK    GeneRIF: Inactivation of Minar2 in mice hyperactivates mTOR
            signaling and results in obesity.
REFERENCE   3  (bases 1 to 1826)
  AUTHORS   Gao,G., Guo,S., Zhang,Q., Zhang,H., Zhang,C. and Peng,G.
  TITLE     Kiaa1024L/Minar2 is essential for hearing by regulating cholesterol
            distribution in hair bundles
  JOURNAL   Elife 11, e80865 (2022)
   PUBMED   36317962
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1826)
  AUTHORS   Bademci,G., Lachgar-Ruiz,M., Deokar,M., Zafeer,M.F., Abad,C.,
            Yildirim Baylan,M., Ingham,N.J., Chen,J., Sineni,C.J., Vadgama,N.,
            Karakikes,I., Guo,S., Duman,D., Singh,N., Harlalka,G., Jain,S.P.,
            Chioza,B.A., Walz,K., Steel,K.P., Nasir,J. and Tekin,M.
  TITLE     Mutations in MINAR2 encoding membrane integral NOTCH2-associated
            receptor 2 cause deafness in humans and mice
  JOURNAL   Proc Natl Acad Sci U S A 119 (26), e2204084119 (2022)
   PUBMED   35727972
  REMARK    GeneRIF: Mutations in MINAR2 encoding membrane integral
            NOTCH2-associated receptor 2 cause deafness in humans and mice.
REFERENCE   5  (bases 1 to 1826)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1826)
  AUTHORS   Ho,R.X., Amraei,R., De La Cena,K.O.C., Sutherland,E.G.,
            Mortazavi,F., Stein,T., Chitalia,V. and Rahimi,N.
  TITLE     Loss of MINAR2 impairs motor function and causes Parkinson's
            disease-like symptoms in mice
  JOURNAL   Brain Commun 2 (1), fcaa047 (2020)
   PUBMED   32954300
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1826)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1826)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   9  (bases 1 to 1826)
  AUTHORS   Tuck,E., Estabel,J., Oellrich,A., Maguire,A.K., Adissu,H.A.,
            Souter,L., Siragher,E., Lillistone,C., Green,A.L., Wardle-Jones,H.,
            Carragher,D.M., Karp,N.A., Smedley,D., Adams,N.C., Bussell,J.N.,
            Adams,D.J., Ramirez-Solis,R., Steel,K.P., Galli,A. and White,J.K.
  CONSRTM   Sanger Institute Mouse Genetics Project
  TITLE     A gene expression resource generated by genome-wide lacZ profiling
            in the mouse
  JOURNAL   Dis Model Mech 8 (11), 1467-1478 (2015)
   PUBMED   26398943
REFERENCE   10 (bases 1 to 1826)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132242.2, BY125990.1 and CO044279.1.
            
            On or before Jun 18, 2010 this sequence version replaced
            NM_001167927.1, NM_173759.4.
            
            Transcript Variant: This variant (3) lacks an alternate in-frame
            exon in the 5' coding region, compared to variant 2, resulting in
            an isoform (a) that is shorter than isoform c.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus and to represent an alternate splice pattern from a
            variant that lacks exons 2 and 3. The update changes the protein
            length from 151 aa to 193 aa. The AUG used in the update has some
            conservation, but there is another downstream AUG that is better
            conserved than the first, and which has a stronger Kozak signal,
            and is located 4 codons downstream. This means that the downstream
            AUG may also be used due to leaky scanning.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB234198.1, SRR7652917.780149.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AC132242.2         139280-139424
            146-467             BY125990.1         1-322
            468-1144            AC132242.2         152517-153193
            1145-1826           CO044279.1         1-682               c
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 33.9 cM"
     gene            1..1826
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="membrane integral NOTCH2 associated receptor 2"
                     /db_xref="GeneID:225583"
                     /db_xref="MGI:MGI:2442934"
     exon            1..182
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..587
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="isoform a is encoded by transcript variant 3;
                     UPF0258 protein KIAA1024-like homolog; major intrinsically
                     disordered Notch2-binding receptor 1-like homolog; major
                     intrinsically disordered NOTCH2-associated receptor 2"
                     /codon_start=1
                     /product="major intrinsically disordered NOTCH2-binding
                     receptor 1-like homolog isoform a"
                     /protein_id="NP_776120.3"
                     /db_xref="CCDS:CCDS29267.2"
                     /db_xref="GeneID:225583"
                     /db_xref="MGI:MGI:2442934"
                     /translation="MKGDMDLSVLPNNNHPDKFLQLDVKSLMRSSTLLQASLARFPGG
                     NYPATQHWQNLVYSQREKTIATQRIKGFGVENPVLPESPPASMSSVMKNNPLYGDITL
                     EEAMEERKKSPSWTIEEYDKHSVHTNLSGHLKENPNDLRFWLGDTYTPGFDTLLKKKK
                     KRNKRSKLCHMGLILLLVASILVTIVTLSTIFS"
     exon            183..407
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            408..1812
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1787..1792
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="hexamer: AATAAA"
     regulatory      1791..1796
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="hexamer: AATAAT"
     polyA_site      1807
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="major polyA site"
     polyA_site      1812
                     /gene="Minar2"
                     /gene_synonym="A730017C20Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggtagatgaa aggggacatg gatctttctg ttttgcccaa taacaaccat cctgacaaat
       61 tcctgcagct tgacgtgaag tctttaatga ggagctcaac ccttctgcag gctagcctcg
      121 cgagatttcc gggtggaaat tatcctgcca cacaacactg gcaaaacctt gtctactcac
      181 agagagaaaa aaccattgct actcagcgaa tcaagggatt tggtgtggag aacccagtcc
      241 ttcctgagag tcctccagca tccatgtctt cagttatgaa gaacaacccg ttgtatggtg
      301 acataacttt ggaagaagct atggaagaaa ggaaaaagag tccttcatgg accattgagg
      361 agtatgacaa gcattctgtg catacaaatc tctctggaca tctgaaggaa aaccccaacg
      421 acctgcgctt ttggttgggg gatacgtaca ctcctggctt cgacactctg ctgaaaaaga
      481 agaagaaacg caacaagcga tcaaaattgt gtcacatggg tctgatttta ctccttgtgg
      541 cctccattct ggttaccata gtgactctca gcaccatatt ctcttaagga agctgcagac
      601 ccatctttaa agctctaaaa tgccttctaa gtacttgcaa gagaatctgt ctgtgcattg
      661 tgaacgtccg cattgctaac tgtgtgacca aacaccattg tcctttagaa tgtgagcgtc
      721 gcttgtgtta tgttggcctc tctgctttgg tctgtgaaac ctgtgtgtca ttccagtctg
      781 ggtttgtcct ttgtgaagca ggtattccag gggctcacaa tatcaaaagt cactccatac
      841 agttaacaat caagctttag attggaagcg gactgcgcca agtgctggca actgcttgtc
      901 ttcatgctta cctgtttttc acatcattcc tccttcctcc tcctccctcc tccttcctcc
      961 tcctcctctt cctccttctt ttccttctcc tctgggcact cgggaagctg tagctcatct
     1021 gcccaacagc tagggtgacc agaaggcttc tgctgagcta cccctcagtt tagatctgtt
     1081 ttaccctcct ttatctccgc ttaagttaaa ggcggataaa gtcgaaggga gacattgttc
     1141 aaacctattg taatttcccc agtgatcctt tctggaaacc taaattgtat gttgttgttt
     1201 tagcagaaac aaacttggtc agccacagct gcataatcaa ttaagttaca tccaatcgtg
     1261 cttggggatt gataggaact ggcaagtgtg acctctagtg gcagtgggta cacgtttacc
     1321 agaaataggt ggaggggcac tggaaagagt ccaggaatgc tgccaccccc tgagcattca
     1381 attgaatgac agtcaggcct gaacaaaaga ttgtcagaga agggaactgg cttaaggatt
     1441 ctgtacaaca tggaggggag aagagcggtt ggctcctaac ctgtagcttt ttcttcagtg
     1501 gtgatgtcgt tttctttgtg tattgacaac cttctggtgg gtgcagatac ctgggaacaa
     1561 gccccaggat acaaagaacc aaagtgtctg tcttctccct ggacgttctt acctaaggct
     1621 ctcagcgcca ctcccaatgc tatttagtgt gtgaaataag gcttttcttc ttcttcttcc
     1681 caaagagtgc cgtcctctcg tgccccttga taacgcctta ctatctcgct gtattgctta
     1741 taacgatact tgaagtaaag tatataaaaa tgagggttta ctggaaaata aataattgtg
     1801 tacaaagcaa aaaaaaaaaa aaaaaa
//
