LOCUS       NM_001242407            3369 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mitoguardin 2 (Miga2), transcript variant 2, mRNA.
ACCESSION   NM_001242407
VERSION     NM_001242407.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Yan,M.Q., Zhu,B.H., Liu,X.H., Yang,Y.M., Duan,X.Y., Wang,Y.,
            Sun,H., Feng,M., Li,T. and Liu,X.M.
  TITLE     Mitoguardin 1 and 2 promote granulosa cell proliferation by
            activating AKT and regulating the Hippo-YAP1 signaling pathway
  JOURNAL   Cell Death Dis 14 (11), 779 (2023)
   PUBMED   38012141
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3369)
  AUTHORS   Fan,K.Q., Li,Y.Y., Wang,H.L., Mao,X.T., Guo,J.X., Wang,F.,
            Huang,L.J., Li,Y.N., Ma,X.Y., Gao,Z.J., Chen,W., Qian,D.D.,
            Xue,W.J., Cao,Q., Zhang,L., Shen,L., Zhang,L., Tong,C., Zhong,J.Y.,
            Lu,W., Lu,L., Ren,K.M., Zhong,G., Wang,Y., Tang,M., Feng,X.H.,
            Chai,R.J. and Jin,J.
  TITLE     Stress-Induced Metabolic Disorder in Peripheral CD4+ T Cells Leads
            to Anxiety-like Behavior
  JOURNAL   Cell 179 (4), 864-879 (2019)
   PUBMED   31675497
REFERENCE   4  (bases 1 to 3369)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3369)
  AUTHORS   Gao,Z., Li,Y., Wang,F., Huang,T., Fan,K., Zhang,Y., Zhong,J.,
            Cao,Q., Chao,T., Jia,J., Yang,S., Zhang,L., Xiao,Y., Zhou,J.Y.,
            Feng,X.H. and Jin,J.
  TITLE     Mitochondrial dynamics controls anti-tumour innate immunity by
            regulating CHIP-IRF1 axis stability
  JOURNAL   Nat Commun 8 (1), 1805 (2017)
   PUBMED   29180626
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3369)
  AUTHORS   Zhang,Y., Liu,X., Bai,J., Tian,X., Zhao,X., Liu,W., Duan,X.,
            Shang,W., Fan,H.Y. and Tong,C.
  TITLE     Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is
            Required for Neuronal Homeostasis
  JOURNAL   Mol Cell 61 (1), 111-124 (2016)
   PUBMED   26711011
  REMARK    GeneRIF: Propose that MIGA1/2 proteins promote mitochondrial fusion
            by regulating mitochondrial phospholipid metabolism via MitoPLD.
REFERENCE   7  (bases 1 to 3369)
  AUTHORS   Tuck,E., Estabel,J., Oellrich,A., Maguire,A.K., Adissu,H.A.,
            Souter,L., Siragher,E., Lillistone,C., Green,A.L., Wardle-Jones,H.,
            Carragher,D.M., Karp,N.A., Smedley,D., Adams,N.C., Bussell,J.N.,
            Adams,D.J., Ramirez-Solis,R., Steel,K.P., Galli,A. and White,J.K.
  CONSRTM   Sanger Institute Mouse Genetics Project
  TITLE     A gene expression resource generated by genome-wide lacZ profiling
            in the mouse
  JOURNAL   Dis Model Mech 8 (11), 1467-1478 (2015)
   PUBMED   26398943
REFERENCE   8  (bases 1 to 3369)
  AUTHORS   Podrini,C., Koffas,A., Chokshi,S., Vinciguerra,M., Lelliott,C.J.,
            White,J.K., Adissu,H.A., Williams,R. and Greco,A.
  TITLE     MacroH2A1 isoforms are associated with epigenetic markers for
            activation of lipogenic genes in fat-induced steatosis
  JOURNAL   FASEB J 29 (5), 1676-1687 (2015)
   PUBMED   25526730
REFERENCE   9  (bases 1 to 3369)
  AUTHORS   Bassett,J.H., Gogakos,A., White,J.K., Evans,H., Jacques,R.M., van
            der Spek,A.H., Ramirez-Solis,R., Ryder,E., Sunter,D., Boyde,A.,
            Campbell,M.J., Croucher,P.I. and Williams,G.R.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Rapid-throughput skeletal phenotyping of 100 knockout mice
            identifies 9 new genes that determine bone strength
  JOURNAL   PLoS Genet 8 (8), e1002858 (2012)
   PUBMED   22876197
REFERENCE   10 (bases 1 to 3369)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Kitamura,H., Nakagawa,T., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of FLJ
            genes: the complete nucleotide sequences of 110 mouse
            FLJ-homologous cDnas identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (2), 127-135 (2004)
   PUBMED   15449545
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL954388.5.
            
            On May 27, 2022 this sequence version replaced NM_001242407.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK165996.1, AK154200.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-63                AL954388.5         88268-88330
            64-299              AL954388.5         91577-91812
            300-510             AL954388.5         92902-93112
            511-623             AL954388.5         94320-94432
            624-741             AL954388.5         95140-95257
            742-878             AL954388.5         95558-95694
            879-996             AL954388.5         99698-99815
            997-1097            AL954388.5         100671-100771
            1098-1213           AL954388.5         101504-101619
            1214-1286           AL954388.5         101996-102068
            1287-1373           AL954388.5         102199-102285
            1374-1472           AL954388.5         105663-105761
            1473-1607           AL954388.5         105964-106098
            1608-1661           AL954388.5         106991-107044
            1662-1778           AL954388.5         107615-107731
            1779-3369           AL954388.5         107954-109544
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.63 cM"
     gene            1..3369
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /note="mitoguardin 2"
                     /db_xref="GeneID:108958"
                     /db_xref="MGI:MGI:1922035"
     exon            1..63
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            64..299
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..1985
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /note="isoform 1 is encoded by transcript variant 2;
                     ortholog of family with sequence similarity 73, member B
                     FAM73B; mFLJ00199 protein; protein FAM73B"
                     /codon_start=1
                     /product="mitoguardin 2 isoform 1"
                     /protein_id="NP_001229336.1"
                     /db_xref="CCDS:CCDS15880.1"
                     /db_xref="GeneID:108958"
                     /db_xref="MGI:MGI:1922035"
                     /translation="MAFRRTEGMSMIQALAMTVAEIPVFLYTTFGQSAFSQLRLTPGL
                     RKVLFATALGTVALALAAHQLKRRRRKKKQVGPEMGGEQLGTVPMPILMARKVPSVKK
                     GCSSRRVQSPSSKSNDTLSGISSIEPSKHSGSSHSLASMVVVNSSSPTAACSGSWEAR
                     GMEESVPTTDGSAESLYVQGMELFEEALQKWEQALSVGQRGDGGSTPTPGDSLQNPDT
                     ASEALSEPESQRREFAEKLESLLHRAYHLQEEFGSTFPSDSMLLDLERTLMLPLTEGS
                     LRLRADDEDSLTSEDSFFSATEIFESLQIGEYPLPLSRPAAAYEEALQLVKEGRVPCR
                     TLRTELLGCYSDQDFLAKLHCVRQAFEGLLEERSNQIFFGEVGRQMVTGLMTKAEKSP
                     KGFLESYEEMLSYALRPETWATTRLELEGRGVACMSFFDIVLDFILMDAFEDLENPPS
                     SVLAVLRNRWLSDSFKETALATACWSVLKAKRRLLMVPDGFISHFYSVSEHVSPVLAF
                     GFLGPKPQLSEVCAFFKHQIVQYLRDMFDLDNVRYTSVPALAEDILQLSRRRSEILLG
                     YLGAPVASSIGLNGPLPRENGPLEELQ"
     exon            300..510
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            511..623
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            624..741
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            742..878
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            879..996
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1097
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1213
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1286
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1373
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1472
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1607
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1661
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1778
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..3369
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3351..3356
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /note="hexamer: GATAAA"
     polyA_site      3369
                     /gene="Miga2"
                     /gene_synonym="5730472N09Rik; C9orf54; Fam73b"
                     /note="major polyA site"
ORIGIN      
        1 gtggtttggt tgtattgccg cctggaaagg agacgctgcc cttccccagc gatgggatcc
       61 ctgctttgtg gaaggacctg ttatgtgtgc ccctgtcctg ctggggagtg gatggtaccc
      121 aggacccagc cagagaccag ctgaagacat tctccttgca agctctcacc ccaggaggtc
      181 ccgtctcggg tggtggacct accatggcat tccggaggac tgagggtatg tccatgatcc
      241 aggctctggc catgactgtg gctgagattc cagtgttcct gtacacaact tttggtcagt
      301 ccgcgttctc ccagctgcgg ttgacaccgg gcctgaggaa ggttcttttt gccacagccc
      361 ttgggactgt ggccttggcc ctggctgccc accagctgaa aaggcgccgg cggaagaaga
      421 agcaggtggg ccctgagatg ggaggtgagc agctgggcac ggtgcccatg cccatcctca
      481 tggcccgcaa ggtcccttcg gtgaagaaag gctgctccag caggagggtt cagagcccca
      541 gcagcaagag caacgacact cttagtggca tctcctccat cgagcccagc aagcactcag
      601 gctcctccca cagcctggcc tcgatggtag tggtcaattc atccagccct acagctgcgt
      661 gctcaggttc atgggaagcc cgagggatgg aggagtctgt gcccaccact gatggcagtg
      721 ccgagagcct ctatgtgcaa ggcatggagc tgtttgagga agccctgcag aaatgggagc
      781 aggcactgag tgtgggccag agaggggatg gaggcagcac ccccacacca ggcgacagcc
      841 tccagaaccc agacaccgca tcagaagcac tgtcagagcc agagtcccaa cgaagggagt
      901 ttgctgagaa gctggagtcc ctgctgcacc gggcctacca cctgcaggaa gagtttggct
      961 ctaccttccc ctctgacagc atgctgttgg atcttgagag gaccctaatg ctgccactga
     1021 ctgagggctc actgcgactt cgagctgacg atgaggacag cctgacctct gaagactcct
     1081 ttttctcagc cactgagatc tttgagtccc tgcagatcgg cgagtacccg cttcctctct
     1141 ccaggcctgc tgctgcctac gaggaggctc tgcagctggt gaaggaagga agagtccctt
     1201 gccgaacact caggacagag ctgctgggct gctacagtga ccaggacttc ctagccaagc
     1261 tgcattgtgt acggcaggcc tttgaggggc ttctagaaga aagaagcaac cagatcttct
     1321 ttggggaggt cggccggcag atggtgacgg gcctgatgac caaggctgag aagagtccca
     1381 aaggcttcct ggagagctat gaggagatgc tgagctacgc cctgcggcct gagacctggg
     1441 ctaccacccg gctggagctg gagggcagag gggtggcctg catgagcttc ttcgacatcg
     1501 tgctggactt catcctcatg gatgcctttg aggacctaga gaaccctccg tcttcggtgc
     1561 ttgctgtcct gaggaaccgc tggctgtctg acagcttcaa agaaacagcc ctagccaccg
     1621 cgtgctggtc tgtcctgaaa gccaagagga ggctcctgat ggtgcctgat ggcttcatct
     1681 cccatttcta ctccgtatcg gagcatgtta gccctgtgtt ggcctttggc ttcctcggac
     1741 ccaagcccca gctctcggaa gtctgtgctt tcttcaagca ccagattgta caatacctga
     1801 gggacatgtt cgacctggac aacgtgcgct acacatctgt gccagcccta gcagaagaca
     1861 tcctacagct gtcccggcgg cgcagcgaga ttctattggg ctacctgggg gcacctgtgg
     1921 ctagtagcat cgggctgaat gggccactgc ctcgagaaaa tgggcccctg gaagagctgc
     1981 agtaacaggg gatgggctgg aggggagagg tggctgtttg ggcctcagat acaaggtgat
     2041 aggagagaga aagctgccac tcccttcctt tgggttggta tccaagggcc atggtggcca
     2101 agggccattg cccctaagct tgctccactt cctcacctgg gaattcccaa cacccagggg
     2161 acaaatttcc atggagagga accactagaa gcactgatag cagctgccct tggggttccc
     2221 tgagcagcag aggcaggcca gagtcagcag ggactggcca ggggcaggta gcaagagaga
     2281 aaggtgtggg agcaaatggc tcttgagtat ggaccacaga accccagtcc ccccctgctg
     2341 ctgagggagg gcaagggata gttgagggat agaacataca gggctaggtg gtgacagtca
     2401 cagggtcctg caattagcag ccaccaatag ctacattaag tcccagcacc taggcttctg
     2461 gccagtatgg aaagccatgt ctgggccatc cagaggtggg cttgccccat gtgtgctcac
     2521 atctgagcag gtactcaccc ttcacaaacc cttggcgctg ttctccacat tcccacaccc
     2581 tgccaaagat tgtttttatc ccctctgtca ccaaagatgg aggaggcagg actggccgct
     2641 tcctgctctc tggtgagcca ggagctcctg ggcagtgctg ggccccagtc agagctcaca
     2701 gtggcccatc tactgctcca aaagtgagca cggaaccatg gcagggtggg catgggctgc
     2761 acagcgggca gctccccctt cccctttctg acaggaagct ggactcagca ctttgccttc
     2821 aactccatgc acacgtgctt tggggctgcc accacccacg tcctgcagac agttggcatt
     2881 tgtcacggca gctgcagtag tcctggcagc cctccttttg cctgagctgg gaagttttta
     2941 tttattgcct taatacttta tttggctgtt ccatcccttc caggttgtga caccaggaga
     3001 aaagactcct ggtctggagt tgagcactgg ctcctggaac caagttcctc tccctggaag
     3061 ggaaaagggt tccccagggt cagcaggatg cagtaaagag agggcagcca tggctctggg
     3121 tcctctctga ggcttgtgct cagaagggag cagccctcat gcactgtctt gacattccat
     3181 tcatttgcac ttaccctgag ctgtgaatgt gaccctgggc taaggcctgc tctgctctgc
     3241 ccctgaccca tacagtgtgg tattttcatt tttgtcctgt tcatgtgtct atgccccacc
     3301 ctctagcact caaccagcag tggtagagtg tgtgtaaggc ctgtgagtca gataaaggac
     3361 cgaagcgac
//
