LOCUS       NM_174868               5355 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus mitoguardin 1 (Miga1), transcript variant 1, mRNA.
ACCESSION   NM_174868 XM_981415
VERSION     NM_174868.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Gao,Z., Li,Y., Wang,F., Huang,T., Fan,K., Zhang,Y., Zhong,J.,
            Cao,Q., Chao,T., Jia,J., Yang,S., Zhang,L., Xiao,Y., Zhou,J.Y.,
            Feng,X.H. and Jin,J.
  TITLE     Mitochondrial dynamics controls anti-tumour innate immunity by
            regulating CHIP-IRF1 axis stability
  JOURNAL   Nat Commun 8 (1), 1805 (2017)
   PUBMED   29180626
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5355)
  AUTHORS   Liu,X.M., Zhang,Y.L., Ji,S.Y., Zhao,L.W., Shang,W.N., Li,D.,
            Chen,Z., Tong,C. and Fan,H.Y.
  TITLE     Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for
            Ovarian Endocrine Functions and Ovulation
  JOURNAL   Endocrinology 158 (11), 3988-3999 (2017)
   PUBMED   28938432
  REMARK    GeneRIF: The ovarian phenotypes of these mice suggested that
            MIGA1/2 proteins play an important role in ovulation and ovarian
            steroidogenesis. In vivo and in vitro analyses of Miga1/2-knockout
            granulosa cells showed severe defects in luteinization and
            steroidogenesis and disordered mitochondrial morphology and
            function in response to gonadotropins.
REFERENCE   4  (bases 1 to 5355)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 5355)
  AUTHORS   Liu,X.M., Zhang,Y.P., Ji,S.Y., Li,B.T., Tian,X., Li,D., Tong,C. and
            Fan,H.Y.
  TITLE     Mitoguardin-1 and -2 promote maturation and the developmental
            potential of mouse oocytes by maintaining mitochondrial dynamics
            and functions
  JOURNAL   Oncotarget 7 (2), 1155-1167 (2016)
   PUBMED   26716412
  REMARK    GeneRIF: Data indicate that mitoguardin (Miga) knoskout Miga1/2-/-
            females show greatly reduced quality of oocytes and early embryos
            and are subfertile.
REFERENCE   6  (bases 1 to 5355)
  AUTHORS   Zhang,Y., Liu,X., Bai,J., Tian,X., Zhao,X., Liu,W., Duan,X.,
            Shang,W., Fan,H.Y. and Tong,C.
  TITLE     Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is
            Required for Neuronal Homeostasis
  JOURNAL   Mol Cell 61 (1), 111-124 (2016)
   PUBMED   26711011
  REMARK    GeneRIF: Propose that MIGA1/2 proteins promote mitochondrial fusion
            by regulating mitochondrial phospholipid metabolism via MitoPLD.
REFERENCE   7  (bases 1 to 5355)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 5355)
  AUTHORS   Qiu,Z., Liu,M., Chen,Z., Shao,Y., Pan,H., Wei,G., Yu,C., Zhang,L.,
            Li,X., Wang,P., Fan,H.Y., Du,B., Liu,B., Liu,M. and Li,D.
  TITLE     High-efficiency and heritable gene targeting in mouse by
            transcription activator-like effector nucleases
  JOURNAL   Nucleic Acids Res 41 (11), e120 (2013)
   PUBMED   23630316
REFERENCE   9  (bases 1 to 5355)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK085017.1, BC098205.1 and AC123075.5.
            
            On Jun 20, 2009 this sequence version replaced NM_174868.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085017.1, BC098205.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1338              AK085017.1         1-1338
            1339-3423           BC098205.1         1322-3406
            3424-5355           AC123075.5         63767-65698
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 77.1 cM"
     gene            1..5355
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /note="mitoguardin 1"
                     /db_xref="GeneID:215708"
                     /db_xref="MGI:MGI:1924567"
     exon            1..156
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            157..475
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..2179
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein FAM73A; family with sequence similarity 73, member
                     A"
                     /codon_start=1
                     /product="mitoguardin 1 isoform 1"
                     /protein_id="NP_777357.2"
                     /db_xref="CCDS:CCDS17916.1"
                     /db_xref="GeneID:215708"
                     /db_xref="MGI:MGI:1924567"
                     /translation="MSDETVSRSQFSLKTYAVRVFALPVSWYYSLSQIKFSPVAKKLF
                     MVTAVSAVSVIFLAHHFKRRRGKQKGKVLPWEPEHLLLEHTRRAASEKGSSCSSSRQN
                     LTLSLSSTKEKGSQCCNYPNGGLLSRYSGSAQSLGSVQSVNSCHSCACGNSNSWDKAD
                     DDDIRLVNIPVTTPENLYLMGMELFEEALRRWEQALTFRSRQAEDEACSSVKLGAGDA
                     IAEESVDDIISSEFIHKLEALLQRAYRLQEEFEATLGGSDPNSIANDTDKDTDMSLRE
                     TMDELGLPDAMNMDSADLFASATELAEQREAQQTFSLESFCPCPFYEEAMHLVEEGKI
                     YSRVLRTEMLECLGDSDFLAKLHCIRQAFQLILAEADNRSFLAESGRKILSALIVKAR
                     KNPKKFQDVFDEMINFLEQTDHWDSTELELAARGVKNLNFYDVVLDFILMDSFEDLEN
                     PPTSIQSVVNNRWLNSSFKESAVASSCWSVLKQKRQQMKISDGFFAHFYAICEHVSPV
                     LAWGFLGPRNSLYDLCCFFKNQVLFFLKDIFDFEKVRYSSIDTLAEDLTHLLIRRTEL
                     LVTCLGADALRHATTCTSGHSHAVPTALLEAKVQ"
     exon            476..653
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            654..790
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            791..917
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            918..1051
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1175
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1276
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1395
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1396..1468
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1555
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1654
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1789
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1790..1843
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1960
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..5355
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4948..4953
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /note="hexamer: AATAAA"
     polyA_site      4980
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /note="major polyA site"
     regulatory      5329..5334
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
                     /note="hexamer: AATAAA"
     polyA_site      5351
                     /gene="Miga1"
                     /gene_synonym="A930038D06; C030011O14Rik; Fam73a; Mita1"
ORIGIN      
        1 gaagaactcc gcctcccgca gccagaggct gctaggtgcg ggctgcggct gggagaccga
       61 ggaggctcag gtgctggcgg gcgcggggca gagcgggcga cgggccgggg accacaccgc
      121 tcggagcgcg gggcccgcag agcggagaga acggcgacag ggtctcagta tgtatctctg
      181 tctggcctgg aactccccgt gtagaccaca ctgacctcaa actcacggag atctgcctcc
      241 caaaaactgg aagcaaaggc atacagcacc atactcagca agacttcaac tttgcatctt
      301 tcatggataa agcacccaga aatccctgtg gctgattaat tcctgtttca ttgatttaag
      361 attggacaga ggcgacatgt cagacgagac agtcagcaga tcacagttct ccttgaagac
      421 gtacgcagtt agagtgtttg ctctccctgt gtcttggtat tattctctct cccagatcaa
      481 attttcacca gtagctaaga agctgttcat ggtaactgcc gtgagtgctg tgtctgtgat
      541 ttttctggcc catcacttta aaagaagacg gggaaaacag aagggcaaag tgctgccctg
      601 ggaacccgag cacctcctcc ttgagcacac taggagagct gcatcagaaa aaggttcgag
      661 ttgctccagc agtcggcaga atctgacctt gtcgttaagc tccaccaagg agaaaggatc
      721 tcagtgttgc aactacccga atggagggct tctcagcaga tactcgggct ctgcccagag
      781 cctgggctcc gtccagagtg ttaactcctg tcatagttgt gcttgtggaa attctaattc
      841 ctgggacaaa gcagacgatg atgacatcag acttgttaac attcctgtga ccacacctga
      901 gaacttatac ctgatgggta tggaattatt tgaagaggcg ttacgacgat gggaacaagc
      961 tctgactttt cgaagtagac aggctgaaga tgaagcctgc agctctgtta aactgggagc
     1021 cggagatgcc attgcagaag aaagtgtgga tgatattatt agttcagaat tcatccataa
     1081 acttgaagct ctactgcaaa gagcgtatcg tctccaagag gagtttgagg ctacccttgg
     1141 gggatctgac cctaattcca ttgcaaatga tactgataaa gacacagaca tgagcttgag
     1201 ggagactatg gatgagcttg gcctgccaga cgccatgaac atggactcag cagatctctt
     1261 tgcttcagca acagagctcg cagagcagag agaagcgcag cagaccttca gcctggagtc
     1321 tttttgtccc tgccctttct acgaagaagc tatgcattta gttgaagaag gaaaaattta
     1381 ctccagagtg ctgagaacgg agatgctgga gtgcctggga gacagtgact tcctcgccaa
     1441 acttcactgt atccggcagg cttttcagct aatccttgca gaagcagaca acaggtcatt
     1501 ccttgctgaa agtggaagga aaattttgtc agctttaatt gtgaaagcac ggaagaatcc
     1561 aaagaaattt caagatgttt ttgatgaaat gatcaatttt ttagagcaga ctgatcactg
     1621 ggatagcact gagctggaac ttgctgccag aggggtgaaa aatttaaatt tttatgacgt
     1681 ggttctggat tttatattaa tggattcctt tgaagattta gaaaacccac cgacatccat
     1741 acagagcgta gtgaacaatc gctggctcaa ctcttccttc aaagaaagcg ctgtggcttc
     1801 aagttgttgg tcagtgctga aacagaaaag gcaacagatg aagatctcag atggattttt
     1861 cgctcatttt tatgccattt gtgagcatgt cagccctgtt ttagcctggg gcttcttggg
     1921 tcctagaaat tctctctatg acttatgttg cttctttaag aatcaagttc ttttctttct
     1981 caaagacatc ttcgactttg agaaggtgcg ctattcgagc atagacacgc tggcggagga
     2041 cctcacgcac ctactcatcc gccgcacgga gctgctggtc acctgtcttg gtgcagatgc
     2101 cctgaggcac gccaccactt gcacgagtgg acactcccac gccgtgccca ctgcgctctt
     2161 agaagccaaa gtacaataag gaccagaagt ggctggagaa tgctgtccag tgctctaggg
     2221 taacccctga ctgtgagtgc ctcagagtct acagggctga cagcgtggcc tcagggagag
     2281 gacagtctca aggatgggcg gggattgtcc tgcacgtatg caggaggttt gtcgctgaag
     2341 ccatgcgatg gatccagaga gactttttaa taatccttag gtaataattt aagttgtgaa
     2401 gtgatgcgta ttatgttgtg actgtcgctt gaattgaagc cagtgctcta ggagttcatt
     2461 gtcttcccac aatctgcagt gtcatctcaa aacacgtctt gatgctttgc tccttgagaa
     2521 tacactagcc caaggtgact gtatttatgt aataatctac ggcttctagc gtcaacgtac
     2581 ttaaccttgc ctgaaatgta ttcttaacct taggtactgc ttctttaata gaatgtagac
     2641 aatagacgtg tttacctaca gcttgtctgt ttgctaatgt atttgagtca aagaaggcat
     2701 cctcaccgag gtctggtgtg cgagagcacc tgaatgaagc gagcggttta cagcgcacac
     2761 ttggggtgca tcacctgcag tccacaggat attactgttg tcctggaagt tatgagaccc
     2821 tgaagtagtt tatctcatct tccctcagat gaatattaaa cttgaaaata taatttattt
     2881 accaaaaaaa gaattaaaaa tgtatcctct cttagatgtg acttagaggc tggattacag
     2941 tttccgactt tagcgtaaga taatttcctc tctcctttca caaaacagac ttttaatcac
     3001 tcacatagag tttatcacat taaaagtttg tcatgttatg cataaatttt aaaaatactc
     3061 atactttgag cttaggtttt aaaggaattt ttctcttaga tataaaacac aaatatttta
     3121 aatgctatgg gagtgtattt tatgtcctta tgataaatga gcaaccgggt tattataagt
     3181 aaccttccaa gtacactata tattttcaag gtctcattaa ttttgcttta agaccatgta
     3241 ccatgttttc accagtggat tggattcact gactctttat cctgaagtgg gctgcgttga
     3301 ttttaagagt gaagtgtaca aaaggatgtg aaatgggttt ttaaataatg catttttaga
     3361 ttaaagtgcc tagagtaagg ttaatgatga gaaaataaaa agtgaaaaga agtttacttt
     3421 tttaagattg taactgtgta gatacatcac agttatgtaa atcgttatta gatgtaggat
     3481 gtagacccgt gccattagtg tgtcaggctc aggccgtacc cctgtcctca gtgcatgtgg
     3541 cctggccttc tcctctcgcc ctttgtttcc tgaccaccag gcagcagtct gcctgaggac
     3601 aaagccctga gcccgacttt ccttatgctt tcacagcaga tgtttttacc tatgagatgt
     3661 caccgatgtg agtgacacac gtgtgcgtgc cttcctgtta ccacatgagc aagacagttg
     3721 tggattatag tcacttattc attggagatc taagtatttt gtcctgtggt tttgtaaaca
     3781 gagatattgg ggggatcctc cttgaaatag tttggaagaa ttgcactgta tagcagtaat
     3841 cccgcagtgg tgtgagatga tcctgtgggt gactcttcca aaaagtattt gatttctttc
     3901 tgtttttaga ttgcagttaa aaaataaata gattgcccat cttcaagaaa gggttggttt
     3961 gtcaaactca gtgcttaagt tttgatgggc taactaacta gaatataaac aagatcgttt
     4021 gtggcctttg ataaaagaga taagaatgac caaataaaat tttgggagca ataaatgtag
     4081 gagaaaaatc tttggtgggg gtttgggaaa gcttaacttt ttaaaggata atgtcttttt
     4141 aaaaagaaca tctctggctc tgactgttga aaatacttaa gatatacata ccagttttat
     4201 ttgccttaaa atcaaacaga gaagcaatgc tttaacagat aaaaacagaa ggtcaaacta
     4261 ggctagagcc tgttagggaa agaagaaaag gctaacctag tggactcatg gtgttaactg
     4321 aagatagcta ccacatgcaa gatgtgcacg ggcagagagt tgatcctgaa aaaatagcca
     4381 gttcctcttt tctggaagaa aaactgataa aaattcagtc gattgatttt gctttgtctt
     4441 gctatgttct tgttcaacta aaaatgtagt tatttcttcc cgaaggttaa caatactacg
     4501 ttcatggaat aacaaagcag aattctcttc ttttacaaga agccaagttt ttaattctat
     4561 aaaagtcagc tccaaagagc tgtctctgtc tcctgaaata gtttgcacca ggagtctgtt
     4621 gattgtgaag ctgactattg taagtactta caagtgttcc tttgctttaa ggtgtaggct
     4681 ttcctacttg ctttgcagaa gttagcctag aaatgaggaa ggccagagga agggtgagaa
     4741 agtggtagtg aactactccg cggcctgtcg tgaccagact ggaagctatg ttataacact
     4801 ggtttttatt ttttatatga acattattaa taattaacat aattaattgg actgtaattt
     4861 taaagtatga gaattagcct gaattaaaat ctgtgaattt ttaaattaag gaatatgtat
     4921 gtgaaagtaa gtgtttatgt gccctgtaat aaatgcgaat atgaactgct ttgttcttaa
     4981 tgactgtcac tttgttctta atcatgtcac agtctccttg actgacctca gcgtatctgt
     5041 ttcttcactg taaggacgga gctcactaat ctgtataaaa gtgaatccct gcagtggcag
     5101 acaacagcac cgtccctcac aggcactgca gtgagcgtgt tttctgtccc agccaaataa
     5161 accagtgatg gtgtcttagt gactagtgac gtgctggctg cattagtaac gctttctatg
     5221 atgtgtgatc acagacgcct tcgtaggcct gccctttcac atgaggcaca gagggaggca
     5281 aagagagaag aacactgaaa tcaagtgtag aataaatgct accatgggaa taaagacagc
     5341 attgcgatta ataaa
//
