LOCUS       NM_001316699            2580 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus MIER family member 2 (Mier2), transcript variant 1,
            mRNA.
ACCESSION   NM_001316699 XM_006514084
VERSION     NM_001316699.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Nakahara,M., Tateyama,H., Araki,M., Nakagata,N., Yamamura,K. and
            Araki,K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Guo,G., Huss,M., Tong,G.Q., Wang,C., Li Sun,L., Clarke,N.D. and
            Robson,P.
  TITLE     Resolution of cell fate decisions revealed by single-cell gene
            expression analysis from zygote to blastocyst
  JOURNAL   Dev Cell 18 (4), 675-685 (2010)
   PUBMED   20412781
REFERENCE   3  (bases 1 to 2580)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   4  (bases 1 to 2580)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   5  (bases 1 to 2580)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC100708.10.
            
            On Feb 3, 2022 this sequence version replaced NM_001316699.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154672.1, SRR13948564.3672281.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-30                AC100708.10        4926-4955
            31-121              AC100708.10        9623-9713
            122-264             AC100708.10        10350-10492
            265-390             AC100708.10        10720-10845
            391-514             AC100708.10        11120-11243
            515-603             AC100708.10        11587-11675
            604-673             AC100708.10        12305-12374
            674-825             AC100708.10        14833-14984
            826-907             AC100708.10        15259-15340
            908-1002            AC100708.10        15415-15509
            1003-1127           AC100708.10        17308-17432
            1128-1204           AC100708.10        17548-17624
            1205-1625           AC100708.10        18095-18515
            1626-2580           AC100708.10        18830-19784
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2580
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /note="MIER family member 2"
                     /db_xref="GeneID:70427"
                     /db_xref="MGI:MGI:1917677"
     exon            1..30
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1647
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mesoderm induction early response protein 2; mesoderm
                     induction early response 1, family member 2"
                     /codon_start=1
                     /product="mesoderm induction early response protein 2
                     isoform 1"
                     /protein_id="NP_001303628.1"
                     /db_xref="CCDS:CCDS83723.1"
                     /db_xref="GeneID:70427"
                     /db_xref="MGI:MGI:1917677"
                     /translation="MAEASSLERQSPRVASCLVHSLCPREPSLQTTAVVSMGSADHQF
                     NLAELLTQNYNLQEGCAEAPQCPDKPEEELDKDFIDQSSDMPLDELLALYGYESSDPI
                     SEQESEGGDTAPALPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFH
                     NQSGSRFLAGDNGPGSSASSDTEEDALPANKCKKEIMVGPQFQADLNILHLNRHCDKI
                     YENEDQLLWSPSVLPEREVEEFLYRAVKRRWQEMAGPQIPEGEVVKDSEQALYELVKC
                     NFNVEEALRRLRFNVKVIRDGLCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVG
                     ECVEYYYLWKKSERYDYFAQQTRLGRRKFVSSGTTDTEQDLDGLDPDGHARLHSEAGV
                     PVEPLNVDIEAGGLDQPGVGSDDLPSSEPGPRPFQQLDEPPAVPSLQQPTSLAASAEL
                     PPAAAAAPEPGTSPRLPVDLALPEELPLVSSPVALSEDTAEPMAPAQVALSVTEFGLI
                     GIGDVNPFLTGHPACPTSTLHSEPLSQCNVMTC"
     exon            31..121
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            122..264
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            265..390
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            391..514
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            515..603
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            604..673
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            674..825
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            826..907
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1002
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1127
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1204
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1625
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..2580
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2560..2565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /note="hexamer: ATTAAA"
     polyA_site      2580
                     /gene="Mier2"
                     /gene_synonym="2700087H15Rik; mi-er2; mKIAA1193"
                     /note="major polyA site"
ORIGIN      
        1 ctcccgcgcg cacgctcggc catggcggag gcctcctctc tggagaggca gagtcctcgt
       61 gtggcctcct gccttgtaca cagcctgtgt cctcgggagc ccagcttgca gaccacggca
      121 gtggtatcca tgggctctgc cgaccatcag ttcaaccttg ctgagctcct gacgcagaac
      181 tacaacctcc aagaagggtg tgctgaggcc ccgcagtgtc ctgacaagcc ggaggaggag
      241 ctggacaagg acttcattga ccagagcagt gacatgcccc tggacgagct gcttgcactg
      301 tatgggtacg agtcgtcaga ccccatctct gagcaggaga gtgaaggcgg tgacacggct
      361 ccagccctgc cggacatgac cttggacaag gagcagatag ccaaggactt gctttctggg
      421 gaagaagagg aagaaacaca gtcctccgct gatgacctca ccccgtctgt cacctcccat
      481 gaggcctcgg acctcttcca taaccagagt gggtcccggt tcctggctgg tgataatggc
      541 ccaggctcct cagcctcctc tgacactgaa gaggacgcgc ttcctgccaa caagtgcaag
      601 aaggagatca tggtagggcc tcaattccaa gctgacctca acatcctgca cttgaaccga
      661 cattgtgaca agatctatga aaatgaagac cagctgctgt ggagccccag tgtactccca
      721 gagcgggagg tggaggagtt cctgtacagg gcagtgaaac ggaggtggca ggagatggct
      781 ggcccccaga tcccagaggg cgaggtggtg aaggacagtg agcaggcgct gtatgagctc
      841 gtgaagtgta atttcaacgt ggaggaggcc ctgcgcaggc tgaggttcaa cgtaaaagtg
      901 atcagagatg ggctctgtgc ctggagcgag gaggagtgca ggaactttga acatggcttc
      961 cgtgttcatg ggaagaactt tcatctgatt caggccaaca aggtacgcac gcggtccgtg
     1021 ggcgagtgtg tggagtatta ttacctgtgg aagaagtctg agcgctacga ctactttgcc
     1081 cagcagacac ggctgggccg gaggaagttt gtctcctctg gaaccacgga tactgaacag
     1141 gacctagatg ggcttgaccc cgatggccat gcccggctgc attctgaggc aggggtgcct
     1201 gtggagcccc tgaatgtgga cattgaagct ggtggacttg accagcctgg cgtgggctcg
     1261 gatgacctcc cttcctcaga gccaggaccg cgtccattcc agcagctgga cgaaccccct
     1321 gctgtgccct cgctccagca gcccactagc ctggccgcct cagctgaact cccaccggcg
     1381 gctgcagctg ctccagagcc gggcactagc cccaggctgc ctgtggacct tgccctaccc
     1441 gaggagctgc cccttgtgtc cagccctgtg gctctgagcg aggacactgc agagcccatg
     1501 gctccagcac aggtggcctt gtcggtcact gaatttggac tcattggcat tggggatgtg
     1561 aatcccttcc taactggcca cccagcgtgc ccaacctcca cactgcactc ggagcccttg
     1621 tcacagtgca atgtgatgac ctgttgaccc ctggtcatct gggggtacgt gtgctcagat
     1681 tggacgtggc aacactgcca ggcctgcgag tcagtcttcc tgacccccct tcccatgcag
     1741 gcctgtgtga tgccttctct gcttcagaac atggtgggat tcagtgaagt ggccagggcc
     1801 acacagacag accccagacc tcagcgtcca gcaccgccac agccccaggc tgccaccatg
     1861 ccatggagtc agcctggcag ctgggctcga agagggttcg tcccggcatg gagccagcca
     1921 ccggccctca gccagcagaa gctggccagg cggtgctgcc tggggccttg ggcaggactc
     1981 atcccgcacc agcagcctct gcccaggcac cctccacagg ccagctcttt ccccaccaca
     2041 gcggggttgt ggcctccagc ctttgctcag tgtgggactt tggggcacag agccatatac
     2101 ctcgtcctgt ggccgccctt tctcggccct tggctgtctt cacttcagga aaagccgcac
     2161 tttatacccc aactgcctcc tgcctgcact ccccaccctg tgccgtggtg gggcctcagc
     2221 atcccaggct cgaagggcag acggtctgct cccttcctgc ccctctcctt ggggcgtctt
     2281 ggggcacagc tctggaagca ccacccagcc acccacctgt cctgtgggtc tcgtttcttt
     2341 ggaggctcct agcagggtgg ctgggcccat ccagtcctcg gcctccctca ttttatttat
     2401 gttggtgttt acaatttgga gtggggtccc tgggcaggca gctccgcccg gtgttggcag
     2461 agcggtgctg tttgagcctg tgcggctgga ctcgaccgtc cctcgcccca ccgaggacac
     2521 tgtgatgtga gactgccttt gcccaatgct attttattta ttaaacttga tttgaagccc
//
