LOCUS       NM_001009927            2564 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus mitochondrial elongation factor 2 (Mief2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001009927 XM_110964
VERSION     NM_001009927.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Osellame,L.D., Singh,A.P., Stroud,D.A., Palmer,C.S.,
            Stojanovski,D., Ramachandran,R. and Ryan,M.T.
  TITLE     Cooperative and independent roles of the Drp1 adaptors Mff, MiD49
            and MiD51 in mitochondrial fission
  JOURNAL   J Cell Sci 129 (11), 2170-2181 (2016)
   PUBMED   27076521
  REMARK    GeneRIF: MiD51 can suppress Mff-dependent enhancement of Drp1
            GTPase activity.
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Elgass,K.D., Smith,E.A., LeGros,M.A., Larabell,C.A. and Ryan,M.T.
  TITLE     Analysis of ER-mitochondria contacts using correlative fluorescence
            microscopy and soft X-ray tomography of mammalian cells
  JOURNAL   J Cell Sci 128 (15), 2795-2804 (2015)
   PUBMED   26101352
  REMARK    GeneRIF: This paper combines confocal live-cell imaging with
            correlative cryogenic fluorescence microscopy and soft x-ray
            tomography to link MiD49 and MiD51 to the involvement of the
            endoplasmic reticulum in mitochondrial fission.
REFERENCE   3  (bases 1 to 2564)
  AUTHORS   Loson,O.C., Meng,S., Ngo,H., Liu,R., Kaiser,J.T. and Chan,D.C.
  TITLE     Crystal structure and functional analysis of MiD49, a receptor for
            the mitochondrial fission protein Drp1
  JOURNAL   Protein Sci 24 (3), 386-394 (2015)
   PUBMED   25581164
  REMARK    GeneRIF: Determined the crystal structure and fperformed unctional
            analysis of mouse MiD49.
REFERENCE   4  (bases 1 to 2564)
  AUTHORS   Loson,O.C., Liu,R., Rome,M.E., Meng,S., Kaiser,J.T., Shan,S.O. and
            Chan,D.C.
  TITLE     The mitochondrial fission receptor MiD51 requires ADP as a cofactor
  JOURNAL   Structure 22 (3), 367-377 (2014)
   PUBMED   24508339
REFERENCE   5  (bases 1 to 2564)
  AUTHORS   Richter,V., Palmer,C.S., Osellame,L.D., Singh,A.P., Elgass,K.,
            Stroud,D.A., Sesaki,H., Kvansakul,M. and Ryan,M.T.
  TITLE     Structural and functional analysis of MiD51, a dynamin receptor
            required for mitochondrial fission
  JOURNAL   J Cell Biol 204 (4), 477-486 (2014)
   PUBMED   24515348
REFERENCE   6  (bases 1 to 2564)
  AUTHORS   Palmer,C.S., Elgass,K.D., Parton,R.G., Osellame,L.D.,
            Stojanovski,D. and Ryan,M.T.
  TITLE     Adaptor proteins MiD49 and MiD51 can act independently of Mff and
            Fis1 in Drp1 recruitment and are specific for mitochondrial fission
  JOURNAL   J Biol Chem 288 (38), 27584-27593 (2013)
   PUBMED   23921378
  REMARK    GeneRIF: MiD49 and MiD51 can act independently of Mff and Fis1 in
            Drp1 recruitment and suggest that they provide specificity to the
            division of mitochondria.
REFERENCE   7  (bases 1 to 2564)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   8  (bases 1 to 2564)
  AUTHORS   Loson,O.C., Song,Z., Chen,H. and Chan,D.C.
  TITLE     Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in
            mitochondrial fission
  JOURNAL   Mol Biol Cell 24 (5), 659-667 (2013)
   PUBMED   23283981
REFERENCE   9  (bases 1 to 2564)
  AUTHORS   Ricard,G., Molina,J., Chrast,J., Gu,W., Gheldof,N., Pradervand,S.,
            Schutz,F., Young,J.I., Lupski,J.R., Reymond,A. and Walz,K.
  TITLE     Phenotypic consequences of copy number variation: insights from
            Smith-Magenis and Potocki-Lupski syndrome mouse models
  JOURNAL   PLoS Biol 8 (11), e1000543 (2010)
   PUBMED   21124890
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2564)
  AUTHORS   Yan,J., Bi,W. and Lupski,J.R.
  TITLE     Penetrance of craniofacial anomalies in mouse models of
            Smith-Magenis syndrome is modified by genomic sequence surrounding
            Rai1: not all null alleles are alike
  JOURNAL   Am J Hum Genet 80 (3), 518-525 (2007)
   PUBMED   17273973
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY234735.1, BB871610.1 and BC088984.1.
            
            On Jan 26, 2013 this sequence version replaced NM_001009927.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK156917.1, BQ952487.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                BY234735.1         1-45
            46-111              BB871610.1         2-67
            112-2564            BC088984.1         1-2453
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.81 cM"
     gene            1..2564
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /note="mitochondrial elongation factor 2"
                     /db_xref="GeneID:237781"
                     /db_xref="MGI:MGI:2144199"
     exon            1..170
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /inference="alignment:Splign:2.1.0"
     exon            171..323
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /inference="alignment:Splign:2.1.0"
     exon            324..486
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /inference="alignment:Splign:2.1.0"
     exon            487..2522
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2501..2506
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /note="hexamer: AATAAA"
     polyA_site      2522
                     /gene="Mief2"
                     /gene_synonym="Gm11; Smcr7"
                     /note="major polyA site"
ORIGIN      
        1 tcggattgga gcgaaggcct gcttatccag cgtgcctttc gggtcacttg ccgcctcggg
       61 cctcggcgtt cggttcccgg ggtttgaacg ttccattcct aggcgagtcc tgggcctcca
      121 gggtcctact cgccctgtcc ttgggtccat gcgctgacgc tcggacgcag gtgaccatgg
      181 cagagttctc ccagaagcag aggaaacaga gcggcagcga gggcctgggc agtgtggtgg
      241 acttcctctt ggctaatgct cgattggtgc taggtgtggg cggggctgca gtgctgggca
      301 ttgctaccct ggctgtaaag cggctcatcg acagggccac cagccctcca gatgaggatg
      361 acaccaaggg ggacagctgg aaggaactga gcctgctgag agccacatcg ccccaaaagc
      421 cccaaccccc tcctgctgcc ttcagccagc ccctagctac tgggtccccc tccccctctg
      481 tgccagtgga gcccacgccc attcattctc ccacgacacc taagttcagc actatagcac
      541 ccctgtgcct gacgttccag gagaggctgc tggccttcga gcgcaagcat gtgattaccc
      601 cagaagccca cgtgacgttg gcgaaacagc tggctggaga cattgccttg gagctgcagg
      661 cctacttgag gagcaagttc ccagaactgc cctttggtgc actcgtgccg ggtgggcctc
      721 tctacgacgg gctccaggca gggactgctg agcacgtgcg cctgctggcg cctctggagt
      781 tggagccggg gctatggagc ctggtacctg gcgtggacac tgtggccagg gagcctcgat
      841 gctgggctgt acgcaggact cagcttgagt ttcatccccg tgggtgcagc ccatgggacc
      901 gcttcctggt ggggggctac ctctcctccc gagtcttgct ggagctgcta cggaaggccc
      961 tgtcagcctc tgtcaactgg ccagccattg gcagcctgct cgggtgtctg atctggcccg
     1021 atgtggcttc ggaggagctg ctgctcaaag tgcaacacga gtgcctggag ttcactttag
     1081 ctgtgctcat ggtagtccct ggggccagca ctgatgaccg cctgctgctg gcttggcccc
     1141 tagaggggct ggccagcaat ctctggctgc aggatctata tccagtagag accgcccgtt
     1201 tgcgggccct ggatgaccag gatgctggta ctcggcggag gttgttgctg ctgctgtgtg
     1261 gtatctgtcg tggccatccg gctctagtgc ggctgggctg gagccacctg actcaggtgg
     1321 ttctgcatct gggtgaagag gaagtggcct ggactgagga ggccttgggg gaacggtttc
     1381 tgcaagccct ggagtttctg gtgggcagct tggagcaggc tagcctgccc tgtcacttca
     1441 accccagtgt gaacctcttg ggtaacttcc gggaagagga gattgatgac attggctacg
     1501 tgctgtacag cggcctccag gttcctgaaa gcctattcta ggtaaatgag gactgtaact
     1561 gacatgtttt ctgatgctgg agagcagcag ccacctccct cctaggacac tgcagtgtgg
     1621 tttgctttat tttagccaga gtggaggggg agtgacatca cctaagccca cacgtgggca
     1681 tgtgcctggg tgatggtgat caagagtctc aaacccagag actctgggtt gctgacatct
     1741 gagtgtggct ggcctcccct ggctgaccgc tttcagtgcc agccacatta atggccctta
     1801 gccgtctggg gttcatttag ggacttgggc tcaggccacc atcaccagca atgaactcac
     1861 aaagaataag atgtggcttt tgatttccta ggcttcacct aggccaagac tgtttttttt
     1921 gtttttttgt tttttttttt ttttcaaaaa aaagaaaatg ttcttgggtg ggtaacagcc
     1981 ccaggtctcc tggaggcact ccgagggggt cagctggagg acagctgctt agctgctgaa
     2041 ggacagtggc ttcctgatgg tggtgcattt gtttttctga ttgggtcctc aggccttgaa
     2101 gctgaagttg actccctcga gggctccctg ccttataccc cagggaagga aggtctcgtt
     2161 ctcttctgct tggttttctc tatgggccac gaattacctc tgggagaaca acttcctcac
     2221 tggggggctg aactctgtgc tgtctatgca aggcaatccc atagaggctg ctcctagacc
     2281 tcactgcagg gtagaggata cgctcctctc agaggcctgt gtgcagacct gtcctctgca
     2341 gcgtgccaaa atattcatga tagatgtgtg tcctcacctt gcaccctggt ccaggtggca
     2401 tggctggagg agggagactg tctcccctac aaggggggct ttctatttta ggtaccaccc
     2461 ccttaaacac actgaaacaa agccatgcct tgaagatgtg aataaaagga gaaagaactg
     2521 tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
