LOCUS       NM_001416092            5307 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mitochondrial elongation factor 1 (Mief1), transcript
            variant 5, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001416092 XM_030248555
VERSION     NM_001416092.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Zhao,Y., Sun,X., Hu,D., Prosdocimo,D.A., Hoppel,C., Jain,M.K.,
            Ramachandran,R. and Qi,X.
  TITLE     ATAD3A oligomerization causes neurodegeneration by coupling
            mitochondrial fragmentation and bioenergetics defects
  JOURNAL   Nat Commun 10 (1), 1371 (2019)
   PUBMED   30914652
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5307)
  AUTHORS   Osellame,L.D., Singh,A.P., Stroud,D.A., Palmer,C.S.,
            Stojanovski,D., Ramachandran,R. and Ryan,M.T.
  TITLE     Cooperative and independent roles of the Drp1 adaptors Mff, MiD49
            and MiD51 in mitochondrial fission
  JOURNAL   J Cell Sci 129 (11), 2170-2181 (2016)
   PUBMED   27076521
  REMARK    GeneRIF: MiD51 can suppress Mff-dependent enhancement of Drp1
            GTPase activity.
REFERENCE   4  (bases 1 to 5307)
  AUTHORS   Elgass,K.D., Smith,E.A., LeGros,M.A., Larabell,C.A. and Ryan,M.T.
  TITLE     Analysis of ER-mitochondria contacts using correlative fluorescence
            microscopy and soft X-ray tomography of mammalian cells
  JOURNAL   J Cell Sci 128 (15), 2795-2804 (2015)
   PUBMED   26101352
  REMARK    GeneRIF: This paper combines confocal live-cell imaging with
            correlative cryogenic fluorescence microscopy and soft x-ray
            tomography to link MiD49 and MiD51 to the involvement of the
            endoplasmic reticulum in mitochondrial fission.
REFERENCE   5  (bases 1 to 5307)
  AUTHORS   Loson,O.C., Liu,R., Rome,M.E., Meng,S., Kaiser,J.T., Shan,S.O. and
            Chan,D.C.
  TITLE     The mitochondrial fission receptor MiD51 requires ADP as a cofactor
  JOURNAL   Structure 22 (3), 367-377 (2014)
   PUBMED   24508339
  REMARK    GeneRIF: ADP is an essential cofactor for MiD51 during
            mitochondrial fission.
REFERENCE   6  (bases 1 to 5307)
  AUTHORS   Richter,V., Palmer,C.S., Osellame,L.D., Singh,A.P., Elgass,K.,
            Stroud,D.A., Sesaki,H., Kvansakul,M. and Ryan,M.T.
  TITLE     Structural and functional analysis of MiD51, a dynamin receptor
            required for mitochondrial fission
  JOURNAL   J Cell Biol 204 (4), 477-486 (2014)
   PUBMED   24515348
REFERENCE   7  (bases 1 to 5307)
  AUTHORS   Palmer,C.S., Elgass,K.D., Parton,R.G., Osellame,L.D.,
            Stojanovski,D. and Ryan,M.T.
  TITLE     Adaptor proteins MiD49 and MiD51 can act independently of Mff and
            Fis1 in Drp1 recruitment and are specific for mitochondrial fission
  JOURNAL   J Biol Chem 288 (38), 27584-27593 (2013)
   PUBMED   23921378
  REMARK    GeneRIF: MiD49 and MiD51 can act independently of Mff and Fis1 in
            Drp1 recruitment and suggest that they provide specificity to the
            division of mitochondria.
REFERENCE   8  (bases 1 to 5307)
  AUTHORS   Loson,O.C., Song,Z., Chen,H. and Chan,D.C.
  TITLE     Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in
            mitochondrial fission
  JOURNAL   Mol Biol Cell 24 (5), 659-667 (2013)
   PUBMED   23283981
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140267.2.
            
            On Jan 12, 2023 this sequence version replaced XM_030248555.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.328182.1,
                                           SRR10223969.16257.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC140267.2         28732-28830         c
            100-224             AC140267.2         28355-28479         c
            225-323             AC140267.2         28133-28231         c
            324-672             AC140267.2         26760-27108         c
            673-823             AC140267.2         16026-16176         c
            824-1001            AC140267.2         14862-15039         c
            1002-1264           AC140267.2         14410-14672         c
            1265-5307           AC140267.2         9542-13584          c
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.85 cM"
     gene            1..5307
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="mitochondrial elongation factor 1"
                     /db_xref="GeneID:239555"
                     /db_xref="MGI:MGI:2146020"
     exon            1..99
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            100..224
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            225..323
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            324..672
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            673..823
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     CDS             680..2071
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="isoform 1 is encoded by transcript variant 5;
                     mitochondrial dynamic protein of 51 kDa homolog;
                     mitochondrial dynamic protein MID51; Smith-Magenis
                     syndrome chromosome region, candidate 7-like;
                     mitochondrial dynamics protein of 51 kDa homolog;
                     mitochondrial dynamics protein MID51"
                     /codon_start=1
                     /product="mitochondrial dynamics protein MID51 isoform 1"
                     /protein_id="NP_001403021.1"
                     /db_xref="GeneID:239555"
                     /db_xref="MGI:MGI:2146020"
                     /translation="MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATL
                     AVKRMYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPRLLNKDMKAGLSRSLQTLPTDS
                     SAFDTDTFCPPRPKPLARRGQVDLKKSRLRMSLQEKLLSYYRNRAAIPAGEQARAKQA
                     AVDICAELRSFLRAKLPDMPLRDMYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCI
                     PGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINW
                     PAIGSLLDYVIRPAPPPEALTLEVQYEKDKHLVIDFLPSVTLGDTVLVARPHRLAQYD
                     NLWRLSLRPAETARLRALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHL
                     AQEEADWSPDMLADRFLQALRGLISYLEAGVLPSALNPKVNLFAELTPQEIDELGYTL
                     YCSLSEPEVLLQT"
     exon            824..1001
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1264
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..5307
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5285..5290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="hexamer: AATAAA"
     polyA_site      5307
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="major polyA site"
ORIGIN      
        1 gtcgtgcccc ggaagtagag gggccatgtt gatgggtgac ccggagagag gtactgggcg
       61 agtggcgagt cctgaggaaa ggtagcgcgt ggtccgcggc cctccgttgt ttttctgcat
      121 cttttgcatc ccggaagacc ctcagggcca aaggggagct gttctgcagt tgaggaaaaa
      181 gggacagcgg tggcttgccc cggttcgcgc tggaagttag caagggttat ggggaggatt
      241 cctccttcca gcctagtttt gaggttggtg agccctgggc cctgtgctgg caaagtttgc
      301 tgaacgccct gaagtgcaca catagtgtga cgattagtac acgtcggtcc cccatggccc
      361 cttggagccg agaggcagtg ctgtgtctgt accgggctct gctgcgccaa ggccgagagc
      421 ttcgctacac tgatcgagac ttctactttg cttccatccg ccgagaattc aggaaaaatc
      481 agaagcttga gaatctggag gctcgagaga agcagctgga gaagggcctg gtcttcctcc
      541 atagcaaact aggaggcctt atctaggatc ctcttttccc ctcctacccc agtcccaaga
      601 cgaagacaaa ggtgccttca gagacactcc cggctcccca ccccacctca cagatgcatt
      661 aggaagcctc aggtgagcaa tggcaggcgc tggtgagcgc aaagggaaga aggatgacaa
      721 tggcattggt acggccatcg attttgtgct ctccaatgcc cggctggtgc tgggtgtggg
      781 tggagcagct atgttgggca ttgccacact ggcagttaag cggatgtatg accgggcaat
      841 cagtgcccct accagcccta cccgcctaag ccattcaggg aagaggagct gggaggagcc
      901 aaactggatg ggctctcctc gcctattgaa caaggacatg aaggcaggcc tgagccggtc
      961 cctgcagacc cttcccacag actcttctgc ctttgacaca gatacattct gcccaccccg
     1021 gcccaaacca ttggccagga ggggccaggt agacttgaag aagtcacgac tccgcatgtc
     1081 cctgcaggag aaacttcttt cttactaccg gaaccgggca gccatccctg ctggagagca
     1141 ggctcgggcc aagcaagctg cggtggacat atgtgctgag ctccggagct tcctgcgggc
     1201 caagctgcct gatatgccac ttcgggacat gtacttgagt ggcagcctct atgatgatct
     1261 gcaggtggtg acggctgacc atatccaact cattgtcccc cttgtgctgg agcagaacct
     1321 gtggtcatgc atacctgggg aggacaccat catgaatgtc cctggtttct tcctggttcg
     1381 tcgtgagaac ccagagtact ttcctcgtgg tagcagttat tgggaccgat gtgttgtagg
     1441 tggctacctc tccccaaaga cagtggcaga cacctttgag aaagtggtgg caggctccat
     1501 caactggcca gccatagggt ccctcttaga ctatgtgatc cgaccagcac cacccccaga
     1561 ggccctgaca ctagaagttc agtatgagaa ggacaaacat cttgtcattg acttcctgcc
     1621 atcagtaacc cttggtgaca ctgtcttggt ggccagacca caccggttag ctcagtatga
     1681 caacctgtgg cggctgagcc ttcgccctgc tgagacagca cgcttacggg ctttggacca
     1741 ggcggactcc ggctgccggt ctctttgcct caagatcctc aaggccatat gcaagtccac
     1801 tccagctctg ggccacctca ctgccagcca gctaaccaac gtcatcctcc acttggccca
     1861 ggaggaggct gactggtctc cagacatgtt ggctgaccgg tttctacagg ccctgagggg
     1921 actcatcagc tacttggagg ctggggtctt gcccagtgcc ctgaacccta aggtgaacct
     1981 atttgcagag ctcacccctc aagaaataga cgagttggga tataccctct actgctcgct
     2041 gtctgagccg gaggtgctgc tgcagacgta gggcaggtaa aggcccagtc tctccgttag
     2101 aaacacttgg ctacgtggtt ggtgcctcac agggtcccta ggtgcctcct gttttgctgg
     2161 taattgtcct ggtcacttca tgctgatgag attgacgtcc ctgcggtttc cccacccacc
     2221 cctatttttg taaccaagca ctgtgctccc cacttgccgc cttactctag gctgagtttt
     2281 tttgtttttt gtttttttgg tttttttttt tttttttttt ggaacaaaca gagggtgtgg
     2341 agtgctggcc tcagcctttg ggtgttttgt ttttggcccg gttttcttgc ttttctgtct
     2401 gctatgtccg gttctgcccc ttggcctctt gtttgctctc ttctctatct ttctctttct
     2461 tggtcaccct ggtggctctt gtccccagag tacatttgcc taatgaagat gttgcctgta
     2521 gttgatgtca cagaagagtg attgcatgtt tccaggctga gctctgcaga ggaataagac
     2581 agtatttagg tttccagggt gaggctggca aagagcctct gccctggcaa atcagccttt
     2641 gcccatggtt cctggaaaac aagcttatct cctctcccta ccctaagcaa ctcctcttta
     2701 tctgagctaa ggcttttgat cgtggggacg gggcattgac caacactgga ggcgccggct
     2761 gcaactgttt tatcagaggc ttatgaggtg cttccttatg gctgtcagcg gaagctcagg
     2821 gctagcacgc tgaagctgat ttggagagcc tgtgtctcac tgtttgtttc tatgtgtggt
     2881 catcagagtc acacagacaa ggcaggtgtg ggggcttcac aggcagcacc tgtaagatgc
     2941 tggtttcccc ttaattgctg gtagaaggtg gccttgcttt agccacggag gccatgggaa
     3001 ggcaaaagga gggaatgcca gccgaaaccc agtgaccaca tttgctttct tgtcaggctt
     3061 tatctttctt aggtgttgcc ttagagcagg aacataccca gggccagaac ctcccacctg
     3121 acccctgtcc ttgccactat aggtacctcc acaggccaga gggcacatga ccatgccagc
     3181 cctgctccct cacttgactc ctgggaagat tggtgctacc taggaagaga gtacatagat
     3241 aagacccaca gaaaagagtt ggggcttccc accttgctgc taagattgct atatatggcc
     3301 atgtaatcat cctatgggtg ggggaaggga catttggaat gtcatttcta tgtgtccttt
     3361 ttcttccgtt catgacaaga ggctgacagt aactctggtt ctgatttgcc attctagtat
     3421 tgtgttctcc catcaggtct ttctttatct gttcttacta cctctccatc agtgtgattc
     3481 tggtgatgca ctctgtggct gtgttgttcc taatgagggt gacacaaaat gactataaat
     3541 agcactgact ctgcagcaac ccatttgaaa ttagggtaga gacacacacc cctctccaca
     3601 ggttaggagg ttgagctagg ctatatattt agcctttttt cctgttctcc cacacatgtg
     3661 taaagacttc taaaggagtt tctttttgat ttgtataaga tagtatgaga aacgtgataa
     3721 agccaagaac cttaactgaa acatgccctc ctggtagtta ataaaaacaa agtaggagct
     3781 gaaaacatct attgctaagt tggactgagt aggcatggaa gggctggcat agacagtcca
     3841 aggttcttgc tgctgcctgt tggagggaga gctggtagaa tgctgcaaaa ccagatttta
     3901 gagaggagtt agatttcctg tcagaagtat tataagactc ctcagctgcc atctctgaac
     3961 tgattccatt taggatgcca ggcagagtgc aaatgaaccc ccagaacttg aagatgctca
     4021 agattccaaa tgtcctaaat cacagccata tgttagtgaa cttaggaaaa gcaatgaatt
     4081 tatcatttgt cactgtgaat ttaggaaaag caatgaattt attgcctgtc tttatgaaca
     4141 gctttgactt tggactatgc tttgcaatcc aagatcatga cttggccatc ttataagcag
     4201 gaacctctta gaagtcctca gaacaaggac aaatgggacc agctctaacc tggtgggttt
     4261 gtggattagc tgggcatgcc ttctttgctg tcattctttg tcataaagta tgggaggcgg
     4321 ccacatgctc tgggatgctt ggatggaaga aaagatgctc tgccctgctt ccctctttgt
     4381 tgaaagaccc agttggctgt gccactgctc tgcctttctc ttcagttact cccacttttg
     4441 cctgcaggaa attccactgt taggccccta cttctgtttg ggaggtattt tgagagtgtc
     4501 aagattagca gactatatac ttgatgccta aaactgtaca cattaccctg gtaaccaagt
     4561 aaatccaaat ttatttattt atttattttt tggttctttg ttgccctttc ccaaaaatgg
     4621 aattcaggat agcagtattc ttctaagtat caacagttgg aatttaaaaa aataaagaga
     4681 aaaactgttg gagtggccca tcatctccta ctccactgta atccactgca gggctggctc
     4741 tgatggttaa gatgaatccg cttcaggctg ggagatctga gagcaggatg cactgtgtgt
     4801 tcagagcctt ctaccaaggt acatttcctt ggcattatga cttgataaaa ctgagagcat
     4861 ttgctacaac tttagctttt ttagtataaa agctcaagcc aaggtaaatg aggaggccct
     4921 tgggaacaca gtgacccttg taccaggcta ggaggttaca agcccctgag actgagtgtc
     4981 cagaatatca gcatgcaggt tctatgttta actgcaggtg gagtgatgtg ggtgttaact
     5041 tttattttga tcaccttgta gtcttttaaa cccttggtct ctgaggccaa gtccacaact
     5101 tgccttccag tcacttgcct gctggtagaa tggaatcagt cactaatctt tgtgtcctgt
     5161 cttggctggg gccatatagg agcagcttag ctactaaggc ccagagaacc tttatcctcc
     5221 ttctccttga aggagagaaa ttgaactggt gaaatcagcg tcttctgcag catgggaaga
     5281 agaaaataaa aatctccttt ccaacaa
//
