LOCUS       NM_001357659            5182 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mitochondrial elongation factor 1 (Mief1), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001357659 XM_006520946
VERSION     NM_001357659.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Zhao,Y., Sun,X., Hu,D., Prosdocimo,D.A., Hoppel,C., Jain,M.K.,
            Ramachandran,R. and Qi,X.
  TITLE     ATAD3A oligomerization causes neurodegeneration by coupling
            mitochondrial fragmentation and bioenergetics defects
  JOURNAL   Nat Commun 10 (1), 1371 (2019)
   PUBMED   30914652
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5182)
  AUTHORS   Osellame,L.D., Singh,A.P., Stroud,D.A., Palmer,C.S.,
            Stojanovski,D., Ramachandran,R. and Ryan,M.T.
  TITLE     Cooperative and independent roles of the Drp1 adaptors Mff, MiD49
            and MiD51 in mitochondrial fission
  JOURNAL   J Cell Sci 129 (11), 2170-2181 (2016)
   PUBMED   27076521
  REMARK    GeneRIF: MiD51 can suppress Mff-dependent enhancement of Drp1
            GTPase activity.
REFERENCE   4  (bases 1 to 5182)
  AUTHORS   Elgass,K.D., Smith,E.A., LeGros,M.A., Larabell,C.A. and Ryan,M.T.
  TITLE     Analysis of ER-mitochondria contacts using correlative fluorescence
            microscopy and soft X-ray tomography of mammalian cells
  JOURNAL   J Cell Sci 128 (15), 2795-2804 (2015)
   PUBMED   26101352
  REMARK    GeneRIF: This paper combines confocal live-cell imaging with
            correlative cryogenic fluorescence microscopy and soft x-ray
            tomography to link MiD49 and MiD51 to the involvement of the
            endoplasmic reticulum in mitochondrial fission.
REFERENCE   5  (bases 1 to 5182)
  AUTHORS   Loson,O.C., Liu,R., Rome,M.E., Meng,S., Kaiser,J.T., Shan,S.O. and
            Chan,D.C.
  TITLE     The mitochondrial fission receptor MiD51 requires ADP as a cofactor
  JOURNAL   Structure 22 (3), 367-377 (2014)
   PUBMED   24508339
  REMARK    GeneRIF: ADP is an essential cofactor for MiD51 during
            mitochondrial fission.
REFERENCE   6  (bases 1 to 5182)
  AUTHORS   Richter,V., Palmer,C.S., Osellame,L.D., Singh,A.P., Elgass,K.,
            Stroud,D.A., Sesaki,H., Kvansakul,M. and Ryan,M.T.
  TITLE     Structural and functional analysis of MiD51, a dynamin receptor
            required for mitochondrial fission
  JOURNAL   J Cell Biol 204 (4), 477-486 (2014)
   PUBMED   24515348
REFERENCE   7  (bases 1 to 5182)
  AUTHORS   Palmer,C.S., Elgass,K.D., Parton,R.G., Osellame,L.D.,
            Stojanovski,D. and Ryan,M.T.
  TITLE     Adaptor proteins MiD49 and MiD51 can act independently of Mff and
            Fis1 in Drp1 recruitment and are specific for mitochondrial fission
  JOURNAL   J Biol Chem 288 (38), 27584-27593 (2013)
   PUBMED   23921378
  REMARK    GeneRIF: MiD49 and MiD51 can act independently of Mff and Fis1 in
            Drp1 recruitment and suggest that they provide specificity to the
            division of mitochondria.
REFERENCE   8  (bases 1 to 5182)
  AUTHORS   Loson,O.C., Song,Z., Chen,H. and Chan,D.C.
  TITLE     Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in
            mitochondrial fission
  JOURNAL   Mol Biol Cell 24 (5), 659-667 (2013)
   PUBMED   23283981
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140267.2.
            
            On Jan 12, 2023 this sequence version replaced NM_001357659.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.76321.1,
                                           SRR13862062.120312.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-99                AC140267.2         28732-28830         c
            100-198             AC140267.2         28133-28231         c
            199-547             AC140267.2         26760-27108         c
            548-698             AC140267.2         16026-16176         c
            699-876             AC140267.2         14862-15039         c
            877-1139            AC140267.2         14410-14672         c
            1140-5182           AC140267.2         9542-13584          c
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.85 cM"
     gene            1..5182
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="mitochondrial elongation factor 1"
                     /db_xref="GeneID:239555"
                     /db_xref="MGI:MGI:2146020"
     exon            1..99
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            100..198
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            199..547
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            548..698
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     CDS             555..1946
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial dynamic protein of 51 kDa homolog;
                     mitochondrial dynamic protein MID51; Smith-Magenis
                     syndrome chromosome region, candidate 7-like;
                     mitochondrial dynamics protein of 51 kDa homolog;
                     mitochondrial dynamics protein MID51"
                     /codon_start=1
                     /product="mitochondrial dynamics protein MID51 isoform 1"
                     /protein_id="NP_001344588.1"
                     /db_xref="CCDS:CCDS27661.1"
                     /db_xref="GeneID:239555"
                     /db_xref="MGI:MGI:2146020"
                     /translation="MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATL
                     AVKRMYDRAISAPTSPTRLSHSGKRSWEEPNWMGSPRLLNKDMKAGLSRSLQTLPTDS
                     SAFDTDTFCPPRPKPLARRGQVDLKKSRLRMSLQEKLLSYYRNRAAIPAGEQARAKQA
                     AVDICAELRSFLRAKLPDMPLRDMYLSGSLYDDLQVVTADHIQLIVPLVLEQNLWSCI
                     PGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINW
                     PAIGSLLDYVIRPAPPPEALTLEVQYEKDKHLVIDFLPSVTLGDTVLVARPHRLAQYD
                     NLWRLSLRPAETARLRALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHL
                     AQEEADWSPDMLADRFLQALRGLISYLEAGVLPSALNPKVNLFAELTPQEIDELGYTL
                     YCSLSEPEVLLQT"
     exon            699..876
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            877..1139
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..5182
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5160..5165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="hexamer: AATAAA"
     polyA_site      5182
                     /gene="Mief1"
                     /gene_synonym="A230016E22; Smcr7l"
                     /note="major polyA site"
ORIGIN      
        1 gtcgtgcccc ggaagtagag gggccatgtt gatgggtgac ccggagagag gtactgggcg
       61 agtggcgagt cctgaggaaa ggtagcgcgt ggtccgcggg gttatgggga ggattcctcc
      121 ttccagccta gttttgaggt tggtgagccc tgggccctgt gctggcaaag tttgctgaac
      181 gccctgaagt gcacacatag tgtgacgatt agtacacgtc ggtcccccat ggccccttgg
      241 agccgagagg cagtgctgtg tctgtaccgg gctctgctgc gccaaggccg agagcttcgc
      301 tacactgatc gagacttcta ctttgcttcc atccgccgag aattcaggaa aaatcagaag
      361 cttgagaatc tggaggctcg agagaagcag ctggagaagg gcctggtctt cctccatagc
      421 aaactaggag gccttatcta ggatcctctt ttcccctcct accccagtcc caagacgaag
      481 acaaaggtgc cttcagagac actcccggct ccccacccca cctcacagat gcattaggaa
      541 gcctcaggtg agcaatggca ggcgctggtg agcgcaaagg gaagaaggat gacaatggca
      601 ttggtacggc catcgatttt gtgctctcca atgcccggct ggtgctgggt gtgggtggag
      661 cagctatgtt gggcattgcc acactggcag ttaagcggat gtatgaccgg gcaatcagtg
      721 cccctaccag ccctacccgc ctaagccatt cagggaagag gagctgggag gagccaaact
      781 ggatgggctc tcctcgccta ttgaacaagg acatgaaggc aggcctgagc cggtccctgc
      841 agacccttcc cacagactct tctgcctttg acacagatac attctgccca ccccggccca
      901 aaccattggc caggaggggc caggtagact tgaagaagtc acgactccgc atgtccctgc
      961 aggagaaact tctttcttac taccggaacc gggcagccat ccctgctgga gagcaggctc
     1021 gggccaagca agctgcggtg gacatatgtg ctgagctccg gagcttcctg cgggccaagc
     1081 tgcctgatat gccacttcgg gacatgtact tgagtggcag cctctatgat gatctgcagg
     1141 tggtgacggc tgaccatatc caactcattg tcccccttgt gctggagcag aacctgtggt
     1201 catgcatacc tggggaggac accatcatga atgtccctgg tttcttcctg gttcgtcgtg
     1261 agaacccaga gtactttcct cgtggtagca gttattggga ccgatgtgtt gtaggtggct
     1321 acctctcccc aaagacagtg gcagacacct ttgagaaagt ggtggcaggc tccatcaact
     1381 ggccagccat agggtccctc ttagactatg tgatccgacc agcaccaccc ccagaggccc
     1441 tgacactaga agttcagtat gagaaggaca aacatcttgt cattgacttc ctgccatcag
     1501 taacccttgg tgacactgtc ttggtggcca gaccacaccg gttagctcag tatgacaacc
     1561 tgtggcggct gagccttcgc cctgctgaga cagcacgctt acgggctttg gaccaggcgg
     1621 actccggctg ccggtctctt tgcctcaaga tcctcaaggc catatgcaag tccactccag
     1681 ctctgggcca cctcactgcc agccagctaa ccaacgtcat cctccacttg gcccaggagg
     1741 aggctgactg gtctccagac atgttggctg accggtttct acaggccctg aggggactca
     1801 tcagctactt ggaggctggg gtcttgccca gtgccctgaa ccctaaggtg aacctatttg
     1861 cagagctcac ccctcaagaa atagacgagt tgggatatac cctctactgc tcgctgtctg
     1921 agccggaggt gctgctgcag acgtagggca ggtaaaggcc cagtctctcc gttagaaaca
     1981 cttggctacg tggttggtgc ctcacagggt ccctaggtgc ctcctgtttt gctggtaatt
     2041 gtcctggtca cttcatgctg atgagattga cgtccctgcg gtttccccac ccacccctat
     2101 ttttgtaacc aagcactgtg ctccccactt gccgccttac tctaggctga gtttttttgt
     2161 tttttgtttt tttggttttt tttttttttt tttttggaac aaacagaggg tgtggagtgc
     2221 tggcctcagc ctttgggtgt tttgtttttg gcccggtttt cttgcttttc tgtctgctat
     2281 gtccggttct gccccttggc ctcttgtttg ctctcttctc tatctttctc tttcttggtc
     2341 accctggtgg ctcttgtccc cagagtacat ttgcctaatg aagatgttgc ctgtagttga
     2401 tgtcacagaa gagtgattgc atgtttccag gctgagctct gcagaggaat aagacagtat
     2461 ttaggtttcc agggtgaggc tggcaaagag cctctgccct ggcaaatcag cctttgccca
     2521 tggttcctgg aaaacaagct tatctcctct ccctacccta agcaactcct ctttatctga
     2581 gctaaggctt ttgatcgtgg ggacggggca ttgaccaaca ctggaggcgc cggctgcaac
     2641 tgttttatca gaggcttatg aggtgcttcc ttatggctgt cagcggaagc tcagggctag
     2701 cacgctgaag ctgatttgga gagcctgtgt ctcactgttt gtttctatgt gtggtcatca
     2761 gagtcacaca gacaaggcag gtgtgggggc ttcacaggca gcacctgtaa gatgctggtt
     2821 tccccttaat tgctggtaga aggtggcctt gctttagcca cggaggccat gggaaggcaa
     2881 aaggagggaa tgccagccga aacccagtga ccacatttgc tttcttgtca ggctttatct
     2941 ttcttaggtg ttgccttaga gcaggaacat acccagggcc agaacctccc acctgacccc
     3001 tgtccttgcc actataggta cctccacagg ccagagggca catgaccatg ccagccctgc
     3061 tccctcactt gactcctggg aagattggtg ctacctagga agagagtaca tagataagac
     3121 ccacagaaaa gagttggggc ttcccacctt gctgctaaga ttgctatata tggccatgta
     3181 atcatcctat gggtggggga agggacattt ggaatgtcat ttctatgtgt cctttttctt
     3241 ccgttcatga caagaggctg acagtaactc tggttctgat ttgccattct agtattgtgt
     3301 tctcccatca ggtctttctt tatctgttct tactacctct ccatcagtgt gattctggtg
     3361 atgcactctg tggctgtgtt gttcctaatg agggtgacac aaaatgacta taaatagcac
     3421 tgactctgca gcaacccatt tgaaattagg gtagagacac acacccctct ccacaggtta
     3481 ggaggttgag ctaggctata tatttagcct tttttcctgt tctcccacac atgtgtaaag
     3541 acttctaaag gagtttcttt ttgatttgta taagatagta tgagaaacgt gataaagcca
     3601 agaaccttaa ctgaaacatg ccctcctggt agttaataaa aacaaagtag gagctgaaaa
     3661 catctattgc taagttggac tgagtaggca tggaagggct ggcatagaca gtccaaggtt
     3721 cttgctgctg cctgttggag ggagagctgg tagaatgctg caaaaccaga ttttagagag
     3781 gagttagatt tcctgtcaga agtattataa gactcctcag ctgccatctc tgaactgatt
     3841 ccatttagga tgccaggcag agtgcaaatg aacccccaga acttgaagat gctcaagatt
     3901 ccaaatgtcc taaatcacag ccatatgtta gtgaacttag gaaaagcaat gaatttatca
     3961 tttgtcactg tgaatttagg aaaagcaatg aatttattgc ctgtctttat gaacagcttt
     4021 gactttggac tatgctttgc aatccaagat catgacttgg ccatcttata agcaggaacc
     4081 tcttagaagt cctcagaaca aggacaaatg ggaccagctc taacctggtg ggtttgtgga
     4141 ttagctgggc atgccttctt tgctgtcatt ctttgtcata aagtatggga ggcggccaca
     4201 tgctctggga tgcttggatg gaagaaaaga tgctctgccc tgcttccctc tttgttgaaa
     4261 gacccagttg gctgtgccac tgctctgcct ttctcttcag ttactcccac ttttgcctgc
     4321 aggaaattcc actgttaggc ccctacttct gtttgggagg tattttgaga gtgtcaagat
     4381 tagcagacta tatacttgat gcctaaaact gtacacatta ccctggtaac caagtaaatc
     4441 caaatttatt tatttattta ttttttggtt ctttgttgcc ctttcccaaa aatggaattc
     4501 aggatagcag tattcttcta agtatcaaca gttggaattt aaaaaaataa agagaaaaac
     4561 tgttggagtg gcccatcatc tcctactcca ctgtaatcca ctgcagggct ggctctgatg
     4621 gttaagatga atccgcttca ggctgggaga tctgagagca ggatgcactg tgtgttcaga
     4681 gccttctacc aaggtacatt tccttggcat tatgacttga taaaactgag agcatttgct
     4741 acaactttag cttttttagt ataaaagctc aagccaaggt aaatgaggag gcccttggga
     4801 acacagtgac ccttgtacca ggctaggagg ttacaagccc ctgagactga gtgtccagaa
     4861 tatcagcatg caggttctat gtttaactgc aggtggagtg atgtgggtgt taacttttat
     4921 tttgatcacc ttgtagtctt ttaaaccctt ggtctctgag gccaagtcca caacttgcct
     4981 tccagtcact tgcctgctgg tagaatggaa tcagtcacta atctttgtgt cctgtcttgg
     5041 ctggggccat ataggagcag cttagctact aaggcccaga gaacctttat cctccttctc
     5101 cttgaaggag agaaattgaa ctggtgaaat cagcgtcttc tgcagcatgg gaagaagaaa
     5161 ataaaaatct cctttccaac aa
//
