LOCUS       NM_021565               3747 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus midnolin (Midn), transcript variant 1, mRNA.
ACCESSION   NM_021565
VERSION     NM_021565.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Zhong,X., Peddada,N., Moresco,J.J., Wang,J., Jiang,Y., Rios,J.J.,
            Moresco,E.M.Y., Choi,J.H. and Beutler,B.
  TITLE     Viable mutations of mouse midnolin suppress B cell malignancies
  JOURNAL   J Exp Med 221 (6) (2024)
   PUBMED   38625151
  REMARK    GeneRIF: Viable mutations of mouse midnolin suppress B cell
            malignancies.
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Kweon,S.M., Irimia-Dominguez,J., Kim,G., Fueger,P.T., Asahina,K.,
            Lai,K.K., Allende,D.S., Lai,Q.R., Lou,C.H., Tsark,W.M., Yang,J.D.,
            Ng,D.S., Lee,J.S., Tso,P., Huang,W. and Lai,K.K.Y.
  TITLE     Heterozygous midnolin knockout attenuates severity of nonalcoholic
            fatty liver disease in mice fed a Western-style diet high in fat,
            cholesterol, and fructose
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 325 (2), G147-G157 (2023)
   PUBMED   37129245
  REMARK    GeneRIF: Heterozygous midnolin knockout attenuates severity of
            nonalcoholic fatty liver disease in mice fed a Western-style diet
            high in fat, cholesterol, and fructose.
REFERENCE   3  (bases 1 to 3747)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3747)
  AUTHORS   Hofmeister-Brix,A., Kollmann,K., Langer,S., Schultz,J., Lenzen,S.
            and Baltrusch,S.
  TITLE     Identification of the ubiquitin-like domain of midnolin as a new
            glucokinase interaction partner
  JOURNAL   J Biol Chem 288 (50), 35824-35839 (2013)
   PUBMED   24187134
  REMARK    GeneRIF: midnolin plays a role in cellular signaling of adult
            tissues and regulates glucokinase enzyme activity in pancreatic
            beta cells
REFERENCE   5  (bases 1 to 3747)
  AUTHORS   Tsukahara,M., Suemori,H., Noguchi,S., Ji,Z.S. and Tsunoo,H.
  TITLE     Novel nucleolar protein, midnolin, is expressed in the
            mesencephalon during mouse development
  JOURNAL   Gene 254 (1-2), 45-55 (2000)
   PUBMED   10974535
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK164324.1, AB036882.1 and
            AI117487.1.
            
            On Apr 3, 2015 this sequence version replaced NM_021565.1.
            
            Summary: This gene encodes a protein that contains an
            ubiquitin-like domain. This protein may be involved in the
            regulation of brain development as inferred by its high expression
            level in the embryonic midbrain. This protein has been found to
            negatively regulate glucokinase activity and insulin secretion in
            pancreatic beta cells. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Apr 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AK164324.1         1-18
            19-3726             AB036882.1         1-3708
            3727-3747           AI117487.1         466-486
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..3747
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /note="midnolin"
                     /db_xref="GeneID:59090"
                     /db_xref="MGI:MGI:1890222"
     exon            1..78
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            79..710
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             475..2001
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     midbrain nucleolar protein"
                     /codon_start=1
                     /product="midnolin isoform 1"
                     /protein_id="NP_067540.1"
                     /db_xref="CCDS:CCDS24011.1"
                     /db_xref="GeneID:59090"
                     /db_xref="MGI:MGI:1890222"
                     /translation="MEPQPGGARSCRRGAPGGACELNTATESAAPMSLAIHSTTGTRY
                     DLSVPHDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPT
                     VEAGLMSQASRPEQSVMQALESLTETQPPATPGPGRAAGGGFRKYRLILFKRPWHRQG
                     PQSPERGGERPQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAA
                     AAAAARGDSSVATPVSSPCRPVSSAARVPPVSSSPSSPVSPSPVTAGSFRSHAASTTC
                     PEQMDCSPPASSSSTSTPGSSPTPRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQD
                     SSGRPRRDIGTILQILNDLLSATRHYQGMPPSLTQLRCHAQCSPASPAPDLTPKTTSC
                     EKLAATSSTSLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARR
                     DARGPYHWTPSRKAGRSDSSSSGGGGGPSEATGLGLDFEDSVWKPEVNPDIQSEFVVA
                     "
     exon            711..798
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            799..861
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            862..990
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1308
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1450
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..1723
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..3727
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3706..3711
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3727
                     /gene="Midn"
                     /gene_synonym="3000003C15Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaccggcac ggccacctct tgccaggacc catacgcagg tcatctggag cagtggtgac
       61 ccaggaccta cggagccgat cttgcgagcg gcggctgcgg ctgttgctaa gggaagggac
      121 gcgcggggta gcacgacccg agagggatcc aggcaccgtg cgaaccagga tagcttgccc
      181 cgtttctcta ggaccctccc cgtgccgtac ccaggtcgct gtcatccgcc cttgaaaacg
      241 gatcacagac cccaccccgg ggcgcaaccc aaggatccca cacgaccccg gagcaccact
      301 cggattcttg attgccggct caattcctaa cgttgggacc ttccccaagg ggcgcacgca
      361 ctgctcctgt ccctgggcgc ccgcggagcg gaggaggacc ttgtttcggc gggggagtcc
      421 cggctaggat tggaggcgac ccgcaggccc ccagttctcc agcgcgcgcc ggggatggag
      481 ccgcagcccg gcggtgcccg gagctgccgg cgcggggccc ccggcggcgc ctgcgagctg
      541 aacacggcca cggagtcggc cgcacccatg agcctggcga tccacagcac tacggggacc
      601 cgctacgacc tgtcggtgcc ccacgacgag accgtggaag ggctgcgcaa aaggttgtcc
      661 caacgcctca aagtacccaa ggaacgcctg gcgctgcttc acaaagacac ccggctcagt
      721 tcggggaagc tgcaggaatt cggcgtgggg gatgggagca agttgacgct cgtgcccacg
      781 gtggaagctg gcctcatgtc ccaggcctcg aggccggaac agtccgttat gcaagccttg
      841 gaaagtttga ccgagaccca gcccccagcg acacccgggc caggccgggc tgccggagga
      901 ggcttccgga aatacagatt gattttattt aagcgtccgt ggcaccgaca gggaccccag
      961 agcccagaga ggggcggcga gaggccccag gtcagtgact ttctgtcagg ccgctcgcct
     1021 ttgaccctgg ccctgcgagt cggggatcac atgatgtttg tgcagttgca actggcagcc
     1081 cagcatgccc cactccagca ccgccatgtg cttgctgctg ccgcagcagc agccgccgcc
     1141 gcccggggag actccagtgt agccacccca gtgtcttcac cctgtaggcc ggtgtccagt
     1201 gccgcccgtg tacccccagt atccagcagc ccttcctcac ctgtatcccc ctcacctgtc
     1261 actgctggct ccttccgatc ccacgcagcc tccacaacct gtcctgagca gatggactgt
     1321 tctccacccg ccagcagcag ctccacatcc accccaggca gcagccccac ccctcgatcc
     1381 cgcaaacctg gtgcggtcat tgagagcttc gtgaaccatg ctccaggggt cttctcaggg
     1441 accttctctg gcacactaca ccccaactgc caggacagca gtgggcggcc tcggcgtgac
     1501 atcggcacca ttctgcagat actcaatgac ctcctaagtg ccacgaggca ctaccagggc
     1561 atgccaccct cactgaccca gctccgctgc catgctcagt gctcacctgc ctcaccagcc
     1621 cctgacctca cccccaaaac tacctcctgt gaaaagctgg cagccacctc ctccacatcc
     1681 ctgctccagg gccagagcca gattcgaatg tgcaagcccc ctggagaccg tcttcgacag
     1741 acagagaacc gtgccacacg ttgcaaagtg gaacgcctcc agctcctgtt gcagcagaag
     1801 cgcctgcgca ggaaggcacg gcgggacgcc cgggggcctt accactggac ccccagccgc
     1861 aaagctggcc gtagcgacag cagcagcagt gggggtggag gtggccccag cgaggccaca
     1921 ggcttgggcc tcgacttcga ggactccgtt tggaagcctg aagtcaaccc ggacatccag
     1981 tccgagtttg tggtggctta aaggatccag tccccttggg tctgtacatc ccctatccca
     2041 tacacggggc aggaggacgc cacagactgg acgctgagcg gcccagctct ggggggcacg
     2101 aggtcacctc cgatgccact taccagcctt tctgattctt ttttagctcc ccgattgtct
     2161 accccgcatc cctggtcaca tgcccactct attcccttac catggtcacg gcctctgcaa
     2221 cagggccctt ccctcccacc tctccagctc caaatatgta gcagtctgtt cccggatggc
     2281 ccagacggag gaagccaccc accccagccc ttccgctctg cctagctctc cccaccccct
     2341 tccataaccc aattcaggtt tttaactcaa tgtagattct ccaagcactt tacagatgag
     2401 ggttggggac cccaggatga gtcccacccc cacccccacc ccagtctgca gacttggcca
     2461 ctgttccagg ccagctggat cccctgcccc tcccaccccg gaaagctgat agactcacag
     2521 aggatacctt tgtccacgaa gcccacttaa ctcacagcct tcctcctgcc tcagtctccc
     2581 aagtgctaca atgacgggcc tttgccacta tgcttggctg gccttgctct tgccacaaaa
     2641 gctcgttgga tggaaaaagc tgaagcaagt ggctgttctc tgatcaggcg ggggatgcct
     2701 cttttcctcg cgaggcggcc atcctctctg gacactggcc tcgccgtctc tttcctccct
     2761 ccacacaccc acttatttcc ttaaccttct ttcccaatgg agcttggggg actatttata
     2821 gatttcattt ttctcactga gccagtgatg gttgctatcc tctaggaaaa aaaaagaaac
     2881 tggggtaggt tgtaggaggg gggcccagaa agacacccct gtacctcttc tccaggccca
     2941 gcctgccact ggggatggga agaggggttc tggactgtgc ctgcattaac tccacgctgg
     3001 ggagggcgcg ctgctggtcc acattggctc catgggggag ccctcccctt gagttggggc
     3061 tgatatagca ggagtgaatc ttgtggggga gtttttttcc tccacatttt gagattttgt
     3121 gataaatggg atgtgtgccc acccctgaca tctgtccatg ttgtctagac tccacctgtc
     3181 cacggttcct ttgttctagg ctgtgaaccc gtccttgtct cttcccccac tgtgtgttta
     3241 aaccttaatg gtttgggatg tgaacgtgtg ttggctagct gtaaaggttt tttggggtgg
     3301 gtgggtggga aggggatcct gaacggtaga ggccaagcga gaattcggtt tggttttttt
     3361 ttttttccta tttttttttc caaaaagaaa agggaggggg gtgtgagtgg gtgcattttt
     3421 taaagctgtc ttgaatacct atttaaatgt gtgttttggg gtgttttttt tagggttttt
     3481 tttttaagct gcctgtgata tgtccactgg gctgagattg gaggcctcag gcaggagccc
     3541 acccatagtt ctcttcccta tgacgacttc cccagatagc attagccacc ctggggggca
     3601 gggggggaag aatccggctg tggggtatcc acctcgccca agtctccttt tactgaaata
     3661 aaatttgagg ggaagagaaa aaacttacac gagcaccgaa tttcaaataa acgaaaatga
     3721 gacatgaaaa aaaaaaaaaa aaaaaaa
//
