LOCUS       NM_011845               6014 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.
ACCESSION   NM_011845 XM_017318482 XM_906569 XM_919909
VERSION     NM_011845.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Gholkar,A.A., Senese,S., Lo,Y.C., Vides,E., Contreras,E.,
            Hodara,E., Capri,J., Whitelegge,J.P. and Torres,J.Z.
  TITLE     The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase
            Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote
            Cell Division
  JOURNAL   Cell Rep 14 (2), 180-188 (2016)
   PUBMED   26748699
REFERENCE   3  (bases 1 to 6014)
  AUTHORS   Li,B., Zhou,T. and Zou,Y.
  TITLE     Mid1/Mid2 expression in craniofacial development and a literature
            review of X-linked opitz syndrome
  JOURNAL   Mol Genet Genomic Med 4 (1), 95-105 (2015)
   PUBMED   26788540
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6014)
  AUTHORS   Boding,L., Hansen,A.K., Meroni,G., Levring,T.B., Woetmann,A.,
            Odum,N., Bonefeld,C.M. and Geisler,C.
  TITLE     MID2 can substitute for MID1 and control exocytosis of lytic
            granules in cytotoxic T cells
  JOURNAL   APMIS 123 (8), 682-687 (2015)
   PUBMED   25924778
  REMARK    GeneRIF: both MID1 and MID2 play a central role in the regulation
            of granule exocytosis and that functional redundancy exists between
            MID1 and MID2 in cytotoxic T cells
REFERENCE   5  (bases 1 to 6014)
  AUTHORS   Reymond,A., Meroni,G., Fantozzi,A., Merla,G., Cairo,S., Luzi,L.,
            Riganelli,D., Zanaria,E., Messali,S., Cainarca,S., Guffanti,A.,
            Minucci,S., Pelicci,P.G. and Ballabio,A.
  TITLE     The tripartite motif family identifies cell compartments
  JOURNAL   EMBO J 20 (9), 2140-2151 (2001)
   PUBMED   11331580
REFERENCE   6  (bases 1 to 6014)
  AUTHORS   Perry,J., Short,K.M., Romer,J.T., Swift,S., Cox,T.C. and
            Ashworth,A.
  TITLE     FXY2/MID2, a gene related to the X-linked Opitz syndrome gene
            FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing
            protein that associates with microtubules
  JOURNAL   Genomics 62 (3), 385-394 (1999)
   PUBMED   10644436
REFERENCE   7  (bases 1 to 6014)
  AUTHORS   Buchner,G., Montini,E., Andolfi,G., Quaderi,N., Cainarca,S.,
            Messali,S., Bassi,M.T., Ballabio,A., Meroni,G. and Franco,B.
  TITLE     MID2, a homologue of the Opitz syndrome gene MID1: similarities in
            subcellular localization and differences in expression during
            development
  JOURNAL   Hum Mol Genet 8 (8), 1397-1407 (1999)
   PUBMED   10400986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683809.7 and BX470203.6.
            
            On Mar 29, 2022 this sequence version replaced NM_011845.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777535.1550825.1,
                                           SRR1660821.60243.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                AL683809.7         144521-144586
            67-782              AL683809.7         157940-158655
            783-878             AL683809.7         173011-173106
            879-986             BX470203.6         5192-5299
            987-1135            BX470203.6         7077-7225
            1136-1263           BX470203.6         18329-18456
            1264-1407           BX470203.6         19579-19722
            1408-1569           BX470203.6         29942-30103
            1570-1777           BX470203.6         31672-31879
            1778-6014           BX470203.6         32236-36472
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 61.35 cM"
     gene            1..6014
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="midline 2"
                     /db_xref="GeneID:23947"
                     /db_xref="MGI:MGI:1344333"
     exon            1..66
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            67..782
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..2180
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /EC_number="2.3.2.27"
                     /note="isoform 3 is encoded by transcript variant 3;
                     probable E3 ubiquitin-protein ligase MID2; midline defect
                     2; tripartite motif-containing protein 1; RING-type E3
                     ubiquitin transferase MID2"
                     /codon_start=1
                     /product="probable E3 ubiquitin-protein ligase MID2
                     isoform 3"
                     /protein_id="NP_035975.1"
                     /db_xref="CCDS:CCDS41151.1"
                     /db_xref="GeneID:23947"
                     /db_xref="MGI:MGI:1344333"
                     /translation="METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSS
                     GESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRR
                     ERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHR
                     LVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEK
                     LKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQR
                     KQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKN
                     IAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELC
                     TASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTV
                     HGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEK
                     DESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNE
                     WIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSL
                     HLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMK
                     ACH"
     exon            783..878
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            879..986
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1135
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1263
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1407
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1408..1569
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1777
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..6014
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5992..5997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="hexamer: AATAAA"
     polyA_site      6014
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="major polyA site"
ORIGIN      
        1 ccccttctcc ctgtgtcttc catcaagatg acagggtgct ggagaaaagg ctgctggaat
       61 cagctggtga gagcccagcc tctgcggttc ttaatgcctc agcaggatta ttttcactaa
      121 agatggaaac actggagtca gaattgacct gtccaatctg cctcgagttg tttgaagacc
      181 cccttctgct tccttgtgct cacagcctat gttttagctg tgcccaccgc atcttggtct
      241 caagctgcag ctctggtgaa tccattgaac ccattactgc tttccagtgt cctacatgca
      301 ggtatgttat ctcgctgaat caccggggcc tggatggcct caagaggaat gtgaccctgc
      361 agaacattat tgatcgcttc cagaaggctt cagtcagtgg gcccaattct ccaagtgaga
      421 gccgccggga gaggacttac aggcctagct ccgccatgtc gagtgagaga attgcatgtc
      481 aattctgtga gcaggaccct ccgagagatg ctgtaaagac gtgcatcacc tgtgaggtct
      541 cctactgtga ccgttgcctt cgggccacac accccaacaa gaaacctttc accagccatc
      601 gcctggtgga accagtttca gacacacatc ttcgagggat tacctgcctg gaccacgaga
      661 atgagaaggt gaacatgtac tgtgtatctg atgatcaatt gatctgtgcc ttatgcaaac
      721 tggtgggtcg tcaccgagac catcaggtcg cttctctgaa tgatcgattt gagaaactaa
      781 agcaaactct cgagatgaac ctcaccaacc tggttaagcg caacagtgaa ctagaaaatc
      841 aaatggccaa actaatacag atctgccagc aagttgaggt gaatactgct atgcatgagg
      901 caaaacttat ggaagaatgt gacgagttgg tagagatcat ccagcagagg aaacaaatga
      961 ttgctgtcaa aatcaaagag acaaaggtta tgaaactgag aaagttggca cagcaggttg
     1021 ctaattgccg ccagtgtctt gagcggtcaa cagtcctcat caaccaagct gagcatatcc
     1081 taaaggaaaa tgaccaagca cggtttctgc agtctgcaaa aaacattgcc gagagggtcg
     1141 ctatggcaac tgcatcttcg caagttctga ttccagacat caattttaat gatgcctttg
     1201 aaaactttgc tttagatttt tctagagaaa agaaactatt ggaggggcta gactatttaa
     1261 cagctccaaa cccaccatct atccgagaag agctctgtac tgcttcccat gacaccatta
     1321 cagtccactg gatctcagac gacgaattca gcatcagctc ctatgagctt cagtacacca
     1381 tattcactgg ccaggctaac ttcatcagcc tgtacaattc agtggatagc tggatgattg
     1441 tgcccaacat taagcagaac cattacacag ttcatggact ccagagtggg acacgctaca
     1501 tctttatcgt gaaagccata aatcaagcag gcagccggaa cagcgaaccc accagactaa
     1561 aaacgaacag ccaaccattt aaactagatc ccaaaatgac tcacaagaag ttgaagatct
     1621 ccaatgatgg attgcagatg gagaaggatg agagctctct gaagaagagc cacactccgg
     1681 agagatttag tggcacaggg tgttatgggg cagcaggaaa tatattcatc gacagtggct
     1741 gccactactg ggaggtggtc atgggttctt caacatggta tgctattggt attgcctaca
     1801 aatcagctcc aaagaatgag tggattggca agaatgcctc ttcctgggtc ttctctcgat
     1861 gcaacagtaa cttcgtggtt cggcacaaca acaaggaaat gctggtggat gtgcccccac
     1921 agttgaagcg tctaggtgtc cttctggatt atgacaacaa catgctgtct ttctatgacc
     1981 cagctaactc tctccatctt catacttttg atgttacctt cattcttcca gtttgtccaa
     2041 cattcacaat ctggaacaaa tccctaatga ttctttctgg cttgcctgcc cctgatttta
     2101 ttgattatcc tgagcggcag gaatgcaact gcaggcctca agaatcccct tatgtgtcag
     2161 ggatgaaagc ttgccattaa gtgtcaagag agcatgtaag tcactggctc tccggttcag
     2221 cacttcccct cttaagcctc cattagtgct ccatatatga gatgcaaact aaagtccatt
     2281 ggaagcactc gaaataacta gatatcgtag atttaaattt ttgtgcatta aaaatcatgt
     2341 ttgaattcat gtattgagtt actcagaaca aattaatctg aatagtctgg gtttgtgcca
     2401 ccagggaaga attctagaga gtatctctgt cgtcattgga gaatttaaaa ttcccagtga
     2461 tttgggagta taaatgatac tgttagcaat tagcccttct agtaatggct agtttgacca
     2521 ggtagaattt gcacagtgtc aaattatcat ttattatatg tcacttttta ataaactaat
     2581 aaaagagggg ttttgagaac aacatatacg tgcatatata atatataaaa tgactgaatt
     2641 cattttatga gcaaaatgac agataatttt tatctattat gtatacacaa taaaccttaa
     2701 tttgcccatt taaataagaa atatatggaa aaggaaaata aatgacactg tccattccta
     2761 actcttaggt aaaaccatca tggctagcca gcatttctct ctctttccct tacagccgtt
     2821 ctttcctccc tcttgcctca tgtacaaaca catgcaatca agacacagga agtgaaaaga
     2881 gctcttggtt agatgaggag agatctgggg acaagattgc acaagagatg ctaatgctac
     2941 atagaaattg gattctggta gagaagtgtc tcgtatagcc taatttcatt gatactgctt
     3001 aatggaggag attcatctga attgttaaaa actatcatag actatttcaa agaaatatag
     3061 ttttgttatt ctcaaataca aagttactag acttaggctt aagaatagag ttgtataatg
     3121 gagtttcttc agaactcacc ttacttgaac agtaagaatg gagtgttaca ggttcaacaa
     3181 ctatatgtgt acaaatgtac ttatattttc agacaaagca ctgaaatgct gttttcctcg
     3241 ataggatcat actcattatt tttggaattt tgtttatact attcattcat ttgcatcccc
     3301 tccacgacaa acaaaaacat aaattccatc ctcgttttta tcacagaagt tgagcttcac
     3361 agcctgtgat ttcaccattt ctgttgcgga gattaattgg aaatgccaga gaaatagaac
     3421 cttcttccag gagccaaatc tacttcttgg cacttgtaaa atgattacta ctgtttaatt
     3481 gttaaatgat agtagtagcc attccctgga ttgtatgtag ccttgataca gtgtcaaacg
     3541 ttagcccctc gttctactag catggtggat tcttatttat tcatttgttc aatgatgcca
     3601 cattactgct gcaagaatgc acatattact agaatgcaga ccttggtgtc aaatgtctca
     3661 ctcttatgca caagagacaa catatctctg taaatctcac ctaatgatcc ttacagattg
     3721 tttgcttatc taccacaaaa aaagcaagac ctgtgccaat acaaaagaaa gacttagatt
     3781 tacatgccag gtagcaaagt caggttctct gccttcaaag attatatgta ccgtatagct
     3841 cattaggaaa tgagcaactt cagtaaaatt caacagtgct ggtagttggc ttgtggtatg
     3901 ataaaactcc cagccttgta attgaaatag ccctagcttc tctgccattt acctaacagt
     3961 gtgacctcag gaacgcactt cttcaaacct gtttcctctt ctgtaaaatg atccccagtt
     4021 tatgatgtaa agctgtaaaa aggttagtgt gtatcagttg ccgagcacag cgcctggcat
     4081 tcactatgtg tgccaccaac gttcatctct tccccaacac cattcatttg tttttgtgtt
     4141 ctttacgttt tagtcctggg aacaaagctt tagttccccg ttctactatg gactgcagct
     4201 tagactacct ttcctcatcc catcttctca gctaactagt gacctctgga attggcattt
     4261 gtggctccct gtggcactcc cctggcacac tgagaccctc cccacccagc aataatatgg
     4321 aagtgttttc agaatgccat catgtaaaga gccattttag aagtattgtt agtgatttgt
     4381 tccttaaaac aattgattct tggcccctgc ccctgatata cttaattaaa ctatatgaac
     4441 ataaggaaac ttacatttga gacaagcttc tcaggttgtt ctgaatcaca caaagtttaa
     4501 tgtagaataa taatcttccc tagaatgaga ctaccttggg gtaagcactg tggcttttgt
     4561 tgttgtattt ttaagaccca gaacagtgtc tggaacaaag tagttggctg gctaatgttt
     4621 gttgaaagaa tgtatgaacg aagaaatgaa taattctaat tgctgtttga ctgtgatgtc
     4681 catttgtgat atttgttatt cttgggggca agttggttgc tttagtctgt attgctatta
     4741 ttttgtaaat gtaccagggc ttatacatac aaatgaacat tgttccagat tggcagttat
     4801 catgaagaat tgtctttgga gcacgtggta tcattctacc agttacattt catactttca
     4861 tgaacttcaa tgaacgttgt tcttgaaggt agatttaagt ttttattaca ggcagagtta
     4921 agcctactga ataaactgaa aaattaaatg aggtttgtgg gaagcaataa agcttgactc
     4981 tgaaatgcta gcttctgagg cacgtaatgc cttccaagaa acaaaatggt ggcaaatcac
     5041 tcagagggct atggtgagtt ccagatattt acttcagtaa acttaaaaga ctttcataga
     5101 acactggcaa gggaccagag ggtaactcta gctaaagcca gttgcaccaa tggaacatga
     5161 ccatttaaca tcatcaagaa agatgattct ataaacatgg ttgctgtcgc tttactccat
     5221 ctaactattc agaacttttt ctcctagaaa attattttct gtctgcaatt tatgcttgtt
     5281 tccacttttt atattgtctt attaaagaaa gcacaaactg aagctaagct tgttttgaga
     5341 ctacaaaaga aattttaatg ccacattgat gttcttgcta cttataagag taactgcatc
     5401 tggctgtgtt tttatgatgt aattccattg tgtgtctttg gatgcagtga agttcatatt
     5461 gtaacaaaat cacttatata tacttttcag ctcagacatt ctaggtgaat tctcagttta
     5521 catatttgta tatgactacc tggatggatc agttcatgga tggacttttg aacaagctta
     5581 gatgctggaa ccccttctat caaggaatgg aaaatgtgag gcaggtagac atgaaccact
     5641 cccacactta cttgtagctc ctgaagccaa ttccagcctt agagccattg ggctgtattt
     5701 ggctgtgtat tacaagttgg ccagatttat tgccatcagg tcaggcaaaa ctgcctttta
     5761 tggtgtggtc tgccagcttc agaaatatgc tgtgttattg ttattggcta gaatatttat
     5821 tagactattg ttgaactatt ttttatcaaa tgctttaccc caggcttgtt tgtactggga
     5881 gctctggacc aatagtgtct ctcctagtaa cagaaatatg aacatctgtg gattacacag
     5941 tcttaccctc tctaaagatc ccaacccatc caaatgctat attatgaatt aaataaatat
     6001 atatgtattc atga
//
