LOCUS       NM_001358367            6381 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.
ACCESSION   NM_001358367 XM_006528522
VERSION     NM_001358367.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Gholkar,A.A., Senese,S., Lo,Y.C., Vides,E., Contreras,E.,
            Hodara,E., Capri,J., Whitelegge,J.P. and Torres,J.Z.
  TITLE     The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase
            Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote
            Cell Division
  JOURNAL   Cell Rep 14 (2), 180-188 (2016)
   PUBMED   26748699
REFERENCE   3  (bases 1 to 6381)
  AUTHORS   Li,B., Zhou,T. and Zou,Y.
  TITLE     Mid1/Mid2 expression in craniofacial development and a literature
            review of X-linked opitz syndrome
  JOURNAL   Mol Genet Genomic Med 4 (1), 95-105 (2015)
   PUBMED   26788540
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6381)
  AUTHORS   Boding,L., Hansen,A.K., Meroni,G., Levring,T.B., Woetmann,A.,
            Odum,N., Bonefeld,C.M. and Geisler,C.
  TITLE     MID2 can substitute for MID1 and control exocytosis of lytic
            granules in cytotoxic T cells
  JOURNAL   APMIS 123 (8), 682-687 (2015)
   PUBMED   25924778
  REMARK    GeneRIF: both MID1 and MID2 play a central role in the regulation
            of granule exocytosis and that functional redundancy exists between
            MID1 and MID2 in cytotoxic T cells
REFERENCE   5  (bases 1 to 6381)
  AUTHORS   Reymond,A., Meroni,G., Fantozzi,A., Merla,G., Cairo,S., Luzi,L.,
            Riganelli,D., Zanaria,E., Messali,S., Cainarca,S., Guffanti,A.,
            Minucci,S., Pelicci,P.G. and Ballabio,A.
  TITLE     The tripartite motif family identifies cell compartments
  JOURNAL   EMBO J 20 (9), 2140-2151 (2001)
   PUBMED   11331580
REFERENCE   6  (bases 1 to 6381)
  AUTHORS   Perry,J., Short,K.M., Romer,J.T., Swift,S., Cox,T.C. and
            Ashworth,A.
  TITLE     FXY2/MID2, a gene related to the X-linked Opitz syndrome gene
            FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing
            protein that associates with microtubules
  JOURNAL   Genomics 62 (3), 385-394 (1999)
   PUBMED   10644436
REFERENCE   7  (bases 1 to 6381)
  AUTHORS   Buchner,G., Montini,E., Andolfi,G., Quaderi,N., Cainarca,S.,
            Messali,S., Bassi,M.T., Ballabio,A., Meroni,G. and Franco,B.
  TITLE     MID2, a homologue of the Opitz syndrome gene MID1: similarities in
            subcellular localization and differences in expression during
            development
  JOURNAL   Hum Mol Genet 8 (8), 1397-1407 (1999)
   PUBMED   10400986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL683809.7 and BX470203.6.
            
            On Mar 29, 2022 this sequence version replaced NM_001358367.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14777531.748703.1,
                                           SRR10223969.20103.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-343               AL683809.7         144880-145222
            344-1059            AL683809.7         157940-158655
            1060-1155           AL683809.7         173011-173106
            1156-1263           BX470203.6         5192-5299
            1264-1412           BX470203.6         7077-7225
            1413-1540           BX470203.6         18329-18456
            1541-1774           BX470203.6         19579-19812
            1775-1936           BX470203.6         29942-30103
            1937-2144           BX470203.6         31672-31879
            2145-6381           BX470203.6         32236-36472
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 61.35 cM"
     gene            1..6381
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="midline 2"
                     /db_xref="GeneID:23947"
                     /db_xref="MGI:MGI:1344333"
     exon            1..343
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            344..1059
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1060..1155
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1263
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1412
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1540
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1774
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1936
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2144
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..6381
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6359..6364
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="hexamer: AATAAA"
     polyA_site      6381
                     /gene="Mid2"
                     /gene_synonym="FXY2; Trim1"
                     /note="major polyA site"
ORIGIN      
        1 agtgtggtgc tgcggtggtg tcatggtgct gcctctggac cgagaggcga aaactcttta
       61 agtttagccc gggagaccta gacgcgggaa cgtcgcggtc tccgtgctgt cccccgtggg
      121 gcgcgcgggc tggcggatcc cggctgttgc gcggcggcgg cggcggccgc agcggcgaag
      181 gcgggcggcg gcctagagtg gtggtggcgg cggcggcggc ggcagcagcg acagcggccc
      241 gggagcttgc acgggagccc gagccagctt gctgcggagg ccggcgagcg cccggcgctc
      301 cccggcgcgc gtaggagatg gctggcaccc gggaacaata tgggtgagag cccagcctct
      361 gcggttctta atgcctcagc aggattattt tcactaaaga tggaaacact ggagtcagaa
      421 ttgacctgtc caatctgcct cgagttgttt gaagaccccc ttctgcttcc ttgtgctcac
      481 agcctatgtt ttagctgtgc ccaccgcatc ttggtctcaa gctgcagctc tggtgaatcc
      541 attgaaccca ttactgcttt ccagtgtcct acatgcaggt atgttatctc gctgaatcac
      601 cggggcctgg atggcctcaa gaggaatgtg accctgcaga acattattga tcgcttccag
      661 aaggcttcag tcagtgggcc caattctcca agtgagagcc gccgggagag gacttacagg
      721 cctagctccg ccatgtcgag tgagagaatt gcatgtcaat tctgtgagca ggaccctccg
      781 agagatgctg taaagacgtg catcacctgt gaggtctcct actgtgaccg ttgccttcgg
      841 gccacacacc ccaacaagaa acctttcacc agccatcgcc tggtggaacc agtttcagac
      901 acacatcttc gagggattac ctgcctggac cacgagaatg agaaggtgaa catgtactgt
      961 gtatctgatg atcaattgat ctgtgcctta tgcaaactgg tgggtcgtca ccgagaccat
     1021 caggtcgctt ctctgaatga tcgatttgag aaactaaagc aaactctcga gatgaacctc
     1081 accaacctgg ttaagcgcaa cagtgaacta gaaaatcaaa tggccaaact aatacagatc
     1141 tgccagcaag ttgaggtgaa tactgctatg catgaggcaa aacttatgga agaatgtgac
     1201 gagttggtag agatcatcca gcagaggaaa caaatgattg ctgtcaaaat caaagagaca
     1261 aaggttatga aactgagaaa gttggcacag caggttgcta attgccgcca gtgtcttgag
     1321 cggtcaacag tcctcatcaa ccaagctgag catatcctaa aggaaaatga ccaagcacgg
     1381 tttctgcagt ctgcaaaaaa cattgccgag agggtcgcta tggcaactgc atcttcgcaa
     1441 gttctgattc cagacatcaa ttttaatgat gcctttgaaa actttgcttt agatttttct
     1501 agagaaaaga aactattgga ggggctagac tatttaacag ctccaaaccc accatctatc
     1561 cgagaagagc tctgtactgc ttcccatgac accattacag tccactggat ctcagacgac
     1621 gaattcagca tcagctccta tgagcttcag tacaccatat tcactggcca ggctaacttc
     1681 atcagtaagt catggtgtag ttggggcctg tggccagaga taaggaaatg taaggaagca
     1741 gtaagctgct caagattggc cggtgcgcca cgaggcctgt acaattcagt ggatagctgg
     1801 atgattgtgc ccaacattaa gcagaaccat tacacagttc atggactcca gagtgggaca
     1861 cgctacatct ttatcgtgaa agccataaat caagcaggca gccggaacag cgaacccacc
     1921 agactaaaaa cgaacagcca accatttaaa ctagatccca aaatgactca caagaagttg
     1981 aagatctcca atgatggatt gcagatggag aaggatgaga gctctctgaa gaagagccac
     2041 actccggaga gatttagtgg cacagggtgt tatggggcag caggaaatat attcatcgac
     2101 agtggctgcc actactggga ggtggtcatg ggttcttcaa catggtatgc tattggtatt
     2161 gcctacaaat cagctccaaa gaatgagtgg attggcaaga atgcctcttc ctgggtcttc
     2221 tctcgatgca acagtaactt cgtggttcgg cacaacaaca aggaaatgct ggtggatgtg
     2281 cccccacagt tgaagcgtct aggtgtcctt ctggattatg acaacaacat gctgtctttc
     2341 tatgacccag ctaactctct ccatcttcat acttttgatg ttaccttcat tcttccagtt
     2401 tgtccaacat tcacaatctg gaacaaatcc ctaatgattc tttctggctt gcctgcccct
     2461 gattttattg attatcctga gcggcaggaa tgcaactgca ggcctcaaga atccccttat
     2521 gtgtcaggga tgaaagcttg ccattaagtg tcaagagagc atgtaagtca ctggctctcc
     2581 ggttcagcac ttcccctctt aagcctccat tagtgctcca tatatgagat gcaaactaaa
     2641 gtccattgga agcactcgaa ataactagat atcgtagatt taaatttttg tgcattaaaa
     2701 atcatgtttg aattcatgta ttgagttact cagaacaaat taatctgaat agtctgggtt
     2761 tgtgccacca gggaagaatt ctagagagta tctctgtcgt cattggagaa tttaaaattc
     2821 ccagtgattt gggagtataa atgatactgt tagcaattag cccttctagt aatggctagt
     2881 ttgaccaggt agaatttgca cagtgtcaaa ttatcattta ttatatgtca ctttttaata
     2941 aactaataaa agaggggttt tgagaacaac atatacgtgc atatataata tataaaatga
     3001 ctgaattcat tttatgagca aaatgacaga taatttttat ctattatgta tacacaataa
     3061 accttaattt gcccatttaa ataagaaata tatggaaaag gaaaataaat gacactgtcc
     3121 attcctaact cttaggtaaa accatcatgg ctagccagca tttctctctc tttcccttac
     3181 agccgttctt tcctccctct tgcctcatgt acaaacacat gcaatcaaga cacaggaagt
     3241 gaaaagagct cttggttaga tgaggagaga tctggggaca agattgcaca agagatgcta
     3301 atgctacata gaaattggat tctggtagag aagtgtctcg tatagcctaa tttcattgat
     3361 actgcttaat ggaggagatt catctgaatt gttaaaaact atcatagact atttcaaaga
     3421 aatatagttt tgttattctc aaatacaaag ttactagact taggcttaag aatagagttg
     3481 tataatggag tttcttcaga actcacctta cttgaacagt aagaatggag tgttacaggt
     3541 tcaacaacta tatgtgtaca aatgtactta tattttcaga caaagcactg aaatgctgtt
     3601 ttcctcgata ggatcatact cattattttt ggaattttgt ttatactatt cattcatttg
     3661 catcccctcc acgacaaaca aaaacataaa ttccatcctc gtttttatca cagaagttga
     3721 gcttcacagc ctgtgatttc accatttctg ttgcggagat taattggaaa tgccagagaa
     3781 atagaacctt cttccaggag ccaaatctac ttcttggcac ttgtaaaatg attactactg
     3841 tttaattgtt aaatgatagt agtagccatt ccctggattg tatgtagcct tgatacagtg
     3901 tcaaacgtta gcccctcgtt ctactagcat ggtggattct tatttattca tttgttcaat
     3961 gatgccacat tactgctgca agaatgcaca tattactaga atgcagacct tggtgtcaaa
     4021 tgtctcactc ttatgcacaa gagacaacat atctctgtaa atctcaccta atgatcctta
     4081 cagattgttt gcttatctac cacaaaaaaa gcaagacctg tgccaataca aaagaaagac
     4141 ttagatttac atgccaggta gcaaagtcag gttctctgcc ttcaaagatt atatgtaccg
     4201 tatagctcat taggaaatga gcaacttcag taaaattcaa cagtgctggt agttggcttg
     4261 tggtatgata aaactcccag ccttgtaatt gaaatagccc tagcttctct gccatttacc
     4321 taacagtgtg acctcaggaa cgcacttctt caaacctgtt tcctcttctg taaaatgatc
     4381 cccagtttat gatgtaaagc tgtaaaaagg ttagtgtgta tcagttgccg agcacagcgc
     4441 ctggcattca ctatgtgtgc caccaacgtt catctcttcc ccaacaccat tcatttgttt
     4501 ttgtgttctt tacgttttag tcctgggaac aaagctttag ttccccgttc tactatggac
     4561 tgcagcttag actacctttc ctcatcccat cttctcagct aactagtgac ctctggaatt
     4621 ggcatttgtg gctccctgtg gcactcccct ggcacactga gaccctcccc acccagcaat
     4681 aatatggaag tgttttcaga atgccatcat gtaaagagcc attttagaag tattgttagt
     4741 gatttgttcc ttaaaacaat tgattcttgg cccctgcccc tgatatactt aattaaacta
     4801 tatgaacata aggaaactta catttgagac aagcttctca ggttgttctg aatcacacaa
     4861 agtttaatgt agaataataa tcttccctag aatgagacta ccttggggta agcactgtgg
     4921 cttttgttgt tgtattttta agacccagaa cagtgtctgg aacaaagtag ttggctggct
     4981 aatgtttgtt gaaagaatgt atgaacgaag aaatgaataa ttctaattgc tgtttgactg
     5041 tgatgtccat ttgtgatatt tgttattctt gggggcaagt tggttgcttt agtctgtatt
     5101 gctattattt tgtaaatgta ccagggctta tacatacaaa tgaacattgt tccagattgg
     5161 cagttatcat gaagaattgt ctttggagca cgtggtatca ttctaccagt tacatttcat
     5221 actttcatga acttcaatga acgttgttct tgaaggtaga tttaagtttt tattacaggc
     5281 agagttaagc ctactgaata aactgaaaaa ttaaatgagg tttgtgggaa gcaataaagc
     5341 ttgactctga aatgctagct tctgaggcac gtaatgcctt ccaagaaaca aaatggtggc
     5401 aaatcactca gagggctatg gtgagttcca gatatttact tcagtaaact taaaagactt
     5461 tcatagaaca ctggcaaggg accagagggt aactctagct aaagccagtt gcaccaatgg
     5521 aacatgacca tttaacatca tcaagaaaga tgattctata aacatggttg ctgtcgcttt
     5581 actccatcta actattcaga actttttctc ctagaaaatt attttctgtc tgcaatttat
     5641 gcttgtttcc actttttata ttgtcttatt aaagaaagca caaactgaag ctaagcttgt
     5701 tttgagacta caaaagaaat tttaatgcca cattgatgtt cttgctactt ataagagtaa
     5761 ctgcatctgg ctgtgttttt atgatgtaat tccattgtgt gtctttggat gcagtgaagt
     5821 tcatattgta acaaaatcac ttatatatac ttttcagctc agacattcta ggtgaattct
     5881 cagtttacat atttgtatat gactacctgg atggatcagt tcatggatgg acttttgaac
     5941 aagcttagat gctggaaccc cttctatcaa ggaatggaaa atgtgaggca ggtagacatg
     6001 aaccactccc acacttactt gtagctcctg aagccaattc cagccttaga gccattgggc
     6061 tgtatttggc tgtgtattac aagttggcca gatttattgc catcaggtca ggcaaaactg
     6121 ccttttatgg tgtggtctgc cagcttcaga aatatgctgt gttattgtta ttggctagaa
     6181 tatttattag actattgttg aactattttt tatcaaatgc tttaccccag gcttgtttgt
     6241 actgggagct ctggaccaat agtgtctctc ctagtaacag aaatatgaac atctgtggat
     6301 tacacagtct taccctctct aaagatccca acccatccaa atgctatatt atgaattaaa
     6361 taaatatata tgtattcatg a
//
