LOCUS       NP_034927                680 aa            linear   ROD 08-APR-2025
DEFINITION  E3 ubiquitin-protein ligase Midline-1 isoform 1 [Mus musculus].
ACCESSION   NP_034927 XP_916267 XP_927010
VERSION     NP_034927.2
DBSOURCE    REFSEQ: accession NM_010797.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 680)
  AUTHORS   Zhang,Y., Kang,H.R., Jun,Y., Kang,H., Bang,G., Ma,R., Ju,S.,
            Yoon,D.E., Kim,Y., Kim,K., Kim,J.Y. and Han,K.
  TITLE     Neurodevelopmental disorder-associated CYFIP2 regulates
            membraneless organelles and eIF2alpha phosphorylation via protein
            interactors and actin cytoskeleton
  JOURNAL   Hum Mol Genet 33 (19), 1671-1687 (2024)
   PUBMED   38981622
REFERENCE   2  (residues 1 to 680)
  AUTHORS   Lin,L., Huang,Z., Li,W., Liu,X., Li,X., Gao,S., Chen,J., Yang,C.,
            Min,X., Yang,H., Gong,Q., Wei,Y., Tu,S., Rao,X., Zhang,Z., Dong,L.
            and Zhong,J.
  TITLE     Mid1 promotes synovitis in rheumatoid arthritis via
            ubiquitin-dependent post-translational modification
  JOURNAL   Pharmacol Res 205, 107224 (2024)
   PUBMED   38777113
  REMARK    GeneRIF: Mid1 promotes synovitis in rheumatoid arthritis via
            ubiquitin-dependent post-translational modification.
REFERENCE   3  (residues 1 to 680)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 680)
  AUTHORS   Wei,Y., Li,W., Huang,J., Braunstein,Z., Liu,X., Li,X., Deiuliis,J.,
            Chen,J., Min,X., Yang,H., Gong,Q., He,L., Liu,Z., Dong,L. and
            Zhong,J.
  TITLE     Midline-1 regulates effector T cell motility in experimental
            autoimmune encephalomyelitis via mTOR/microtubule pathway
  JOURNAL   Theranostics 14 (3), 1168-1180 (2024)
   PUBMED   38323310
  REMARK    GeneRIF: Midline-1 regulates effector T cell motility in
            experimental autoimmune encephalomyelitis via mTOR/microtubule
            pathway.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 680)
  AUTHORS   Cheffer,A., Garcia-Miralles,M., Maier,E., Akol,I., Franz,H.,
            Srinivasan,V.S.V. and Vogel,T.
  TITLE     DOT1L deletion impairs the development of cortical
            parvalbumin-expressing interneurons
  JOURNAL   Cereb Cortex 33 (19), 10272-10285 (2023)
   PUBMED   37566909
REFERENCE   6  (residues 1 to 680)
  AUTHORS   Perry,J. and Ashworth,A.
  TITLE     Evolutionary rate of a gene affected by chromosomal position
  JOURNAL   Curr Biol 9 (17), 987-989 (1999)
   PUBMED   10508587
REFERENCE   7  (residues 1 to 680)
  AUTHORS   Van den Veyver,I.B., Cormier,T.A., Jurecic,V., Baldini,A. and
            Zoghbi,H.Y.
  TITLE     Characterization and physical mapping in human and mouse of a novel
            RING finger gene in Xp22
  JOURNAL   Genomics 51 (2), 251-261 (1998)
   PUBMED   9722948
REFERENCE   8  (residues 1 to 680)
  AUTHORS   Dal Zotto,L., Quaderi,N.A., Elliott,R., Lingerfelter,P.A.,
            Carrel,L., Valsecchi,V., Montini,E., Yen,C.H., Chapman,V.,
            Kalcheva,I., Arrigo,G., Zuffardi,O., Thomas,S., Willard,H.F.,
            Ballabio,A., Disteche,C.M. and Rugarli,E.I.
  TITLE     The mouse Mid1 gene: implications for the pathogenesis of Opitz
            syndrome and the evolution of the mammalian pseudoautosomal region
  JOURNAL   Hum Mol Genet 7 (3), 489-499 (1998)
   PUBMED   9467009
REFERENCE   9  (residues 1 to 680)
  AUTHORS   Quaderi,N.A., Schweiger,S., Gaudenz,K., Franco,B., Rugarli,E.I.,
            Berger,W., Feldman,G.J., Volta,M., Andolfi,G., Gilgenkrantz,S.,
            Marion,R.W., Hennekam,R.C., Opitz,J.M., Muenke,M., Ropers,H.H. and
            Ballabio,A.
  TITLE     Opitz G/BBB syndrome, a defect of midline development, is due to
            mutations in a new RING finger gene on Xp22
  JOURNAL   Nat Genet 17 (3), 285-291 (1997)
   PUBMED   9354791
REFERENCE   10 (residues 1 to 680)
  AUTHORS   Palmer,S., Perry,J., Kipling,D. and Ashworth,A.
  TITLE     A gene spans the pseudoautosomal boundary in mice
  JOURNAL   Proc Natl Acad Sci U S A 94 (22), 12030-12035 (1997)
   PUBMED   9342357
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL671865.11, AL672015.7 and CR478112.2.
            
            On Mar 9, 2007 this sequence version replaced NP_034927.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1, 7, and 8 all encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR14777530.423003.1,
                                    SRR12282455.16557329.1 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849380, SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 79.19 cM"
     Protein         1..680
                     /product="E3 ubiquitin-protein ligase Midline-1 isoform 1"
                     /EC_number="2.3.2.27"
                     /note="midin; tripartite motif-containing protein 18; RING
                     finger protein Midline-1; E3 ubiquitin-protein ligase
                     Midline-1; RING-type E3 ubiquitin transferase Midline-1"
                     /calculated_mol_wt=76005
     Region          3..74
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(10,13,25,27,30,33,56,59)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Site            92
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O15344; propagated from
                     UniProtKB/Swiss-Prot (O70583.2)"
     Site            96
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O15344; propagated from
                     UniProtKB/Swiss-Prot (O70583.2)"
     Region          115..164
                     /region_name="Bbox1_MID1_C-I"
                     /note="B-box-type 1 zinc finger found in midline-1 (MID1)
                     and similar proteins; cd19836"
                     /db_xref="CDD:380894"
     Region          168..214
                     /region_name="Bbox2_MID1_C-I"
                     /note="B-box-type 2 zinc finger found in midline-1 (MID1)
                     and similar proteins; cd19822"
                     /db_xref="CDD:380880"
     Region          219..344
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     Region          323..374
                     /region_name="COS"
                     /note="TRIM C-terminal subgroup One Signature domain;
                     pfam18568"
                     /db_xref="CDD:465805"
     Region          395..494
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          484..503
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O70583.2)"
     Region          498..674
                     /region_name="SPRY"
                     /note="SPRY domain; cl02614"
                     /db_xref="CDD:470632"
     Region          516..535
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O70583.2)"
     Site            524
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O15344; propagated from
                     UniProtKB/Swiss-Prot (O70583.2)"
     CDS             1..680
                     /gene="Mid1"
                     /gene_synonym="61B3-R; DXHXS1141; Fxy; Trim18"
                     /coded_by="NM_010797.4:231..2273"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS41215.1"
                     /db_xref="GeneID:17318"
                     /db_xref="MGI:MGI:1100537"
ORIGIN      
        1 metleseltc piclelfedp lllpcahslc fncahrilvs hcatnepves inafqcptcr
       61 hvitlsqrgl dglkrnvtlq niidrfqkas vsgpnspset rrerafdant mssaekvlcq
      121 fcdqdpaqda vktcvtcevs ycdeclkath pnkkpftghr liepipdshi rglmclehed
      181 ekvnmycvtd dqlicalckl vgrhrdhqva alserydklk qnlesnltnl ikrnteletl
      241 lakliqtcqh vevnasrqea klteecdlli eiiqqrrqii gtkikegkvi rlrklaqqia
      301 nckqclersa slisqaehsl kendharflq taknitervs matassqvli peinlndtfd
      361 tfaldfsrek kllecldylt apnppairee lctasydtit vhwtsedefs vvsyelqyti
      421 ftgqanvvnv acdgtcllgs aglcnsadsw mivpnikqnh ytvhglqsgt kyiftvkain
      481 qagsrssepg klktnsqpfr ldpksahrkl kvshdnltve rdessskksh aperfagqgs
      541 ygvagnvfid sgrhywevvt sgstwyaigl ayrsapkhew igknaaswal crchnhwavr
      601 hdgketpiap aphlrrvgvl ldydngsiaf ydalssvhlh tfhaalaqpv cptftvwnkc
      661 ltivtglpip dhldcteqrp
//
