LOCUS       NM_030110               3300 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus mitochondrial calcium uptake family, member 3 (Micu3),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_030110 XM_001473089 XM_357872
VERSION     NM_030110.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Roman,B., Mastoor,Y., Sun,J., Chapoy Villanueva,H., Hinojosa,G.,
            Springer,D., Liu,J.C. and Murphy,E.
  TITLE     MICU3 Regulates Mitochondrial Calcium and Cardiac Hypertrophy
  JOURNAL   Circ Res 135 (1), 26-40 (2024)
   PUBMED   38747181
  REMARK    GeneRIF: MICU3 Regulates Mitochondrial Calcium and Cardiac
            Hypertrophy.
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3300)
  AUTHORS   Roman,B., Mastoor,Y., Zhang,Y., Gross,D., Springer,D., Liu,C.,
            Glancy,B. and Murphy,E.
  TITLE     Loss of mitochondrial Ca2+ uptake protein 3 impairs skeletal muscle
            calcium handling and exercise capacity
  JOURNAL   J Physiol 602 (1), 113-128 (2024)
   PUBMED   38018177
REFERENCE   4  (bases 1 to 3300)
  AUTHORS   Zhou,G., Ye,Q., Xu,Y., He,B., Wu,L., Zhu,G., Xie,J., Yao,L. and
            Xiao,Z.
  TITLE     Mitochondrial calcium uptake 3 mitigates cerebral amyloid
            angiopathy-related neuronal death and glial inflammation by
            reducing mitochondrial dysfunction
  JOURNAL   Int Immunopharmacol 117, 109614 (2023)
   PUBMED   36878048
  REMARK    GeneRIF: Mitochondrial calcium uptake 3 mitigates cerebral amyloid
            angiopathy-related neuronal death and glial inflammation by
            reducing mitochondrial dysfunction.
REFERENCE   5  (bases 1 to 3300)
  AUTHORS   Singh,R., Bartok,A., Paillard,M., Tyburski,A., Elliott,M. and
            Hajnoczky,G.
  TITLE     Uncontrolled mitochondrial calcium uptake underlies the
            pathogenesis of neurodegeneration in MICU1-deficient mice and
            patients
  JOURNAL   Sci Adv 8 (11), eabj4716 (2022)
   PUBMED   35302860
REFERENCE   6  (bases 1 to 3300)
  AUTHORS   Puente,B.N., Sun,J., Parks,R.J., Fergusson,M.M., Liu,C.,
            Springer,D.A., Aponte,A.M., Liu,J.C. and Murphy,E.
  TITLE     MICU3 Plays an Important Role in Cardiovascular Function
  JOURNAL   Circ Res 127 (12), 1571-1573 (2020)
   PUBMED   33059536
REFERENCE   7  (bases 1 to 3300)
  AUTHORS   Patron,M., Granatiero,V., Espino,J., Rizzuto,R. and De Stefani,D.
  TITLE     MICU3 is a tissue-specific enhancer of mitochondrial calcium uptake
  JOURNAL   Cell Death Differ 26 (1), 179-195 (2019)
   PUBMED   29725115
  REMARK    GeneRIF: MICU3 forms a disulfide bond-mediated dimer with MICU1,
            but not with MICU2, and it acts as enhancer of MCU-dependent
            mitochondrial Ca(2+) uptake. Silencing of MICU3 in primary cortical
            neurons impairs Ca(2+) signals elicited by synaptic activity, thus
            suggesting a specific role in regulating neuronal function.
            GeneRIF: MICU3 is a tissue-specific enhancer of mitochondrial
            calcium uptake
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160537.6 and AC102680.10.
            
            On May 31, 2019 this sequence version replaced NM_030110.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1297950.1,
                                           SRR1660815.14359.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-388               AC160537.6         10983-11370         c
            389-542             AC102680.10        82449-82602         c
            543-574             AC102680.10        78083-78114         c
            575-653             AC102680.10        69394-69472         c
            654-701             AC102680.10        66329-66376         c
            702-784             AC102680.10        64027-64109         c
            785-856             AC102680.10        61485-61556         c
            857-895             AC102680.10        58964-59002         c
            896-991             AC102680.10        52168-52263         c
            992-1092            AC102680.10        42775-42875         c
            1093-1264           AC102680.10        42145-42316         c
            1265-1373           AC102680.10        39472-39580         c
            1374-1531           AC102680.10        37583-37740         c
            1532-1600           AC102680.10        36235-36303         c
            1601-3300           AC102680.10        32104-33803         c
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 23.89 cM"
     gene            1..3300
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="mitochondrial calcium uptake family, member 3"
                     /db_xref="GeneID:78506"
                     /db_xref="MGI:MGI:1925756"
     exon            1..388
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1600
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="EF-hand domain-containing family member A2; EF-hand
                     domain family, member A2"
                     /codon_start=1
                     /product="calcium uptake protein 3, mitochondrial"
                     /protein_id="NP_084386.1"
                     /db_xref="CCDS:CCDS52545.1"
                     /db_xref="GeneID:78506"
                     /db_xref="MGI:MGI:1925756"
                     /translation="MAALRRFLWPPPRLSPALAPQQPFLSPWGRPAGTAPGMSGRPFS
                     CREEDEGAVAEAAWRRRRWGELSIAAAAGGGLVGLVCYQLYGDPRADPSELAAPELED
                     PPRGRGLLPIPVAAAKETVATGRAITEDLDLYATSRERRFRLFASIECEGQLFMTPYD
                     FILAVTTDEPKFAKTWKSLSKQELSQMLSETPPVWKGSSKLFRNLKERGVISYTEYLF
                     LLCILTKPHAGFRIAFNMFDTDGNEMVDKKEFLVLQEIFRKKNEKRETKGDEEKRAML
                     RLQLYGYHSPTNSVLKTDAGELVSRSYWDTLRRSTSQALFSDLAERADDITSLVADTT
                     LLVHFFGKKGKAELNFEDFYRFMDNLQTEVLEIEFLSYSNGMNTISEEDFAHILLRYT
                     NVENTSVFLENVRYSISEEKGITFDEFRSFFQFLNNLEDFAIALNMYNFASRSIGQDE
                     FKRAVYVATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMKDRLHRGFRGYKTVQK
                     YPTFKSCLKKELHSR"
     exon            389..542
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            543..574
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            575..653
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            654..701
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            702..784
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..856
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            857..895
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            896..991
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1092
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1264
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1373
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1531
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1600
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..3300
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3277..3282
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="hexamer: AATAAA"
     regulatory      3281..3286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="hexamer: AAGAAA"
     polyA_site      3296
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="major polyA site"
     polyA_site      3300
                     /gene="Micu3"
                     /gene_synonym="2900075B16Rik; Efha2"
                     /note="major polyA site"
ORIGIN      
        1 cggctcggcc cggcccgcag ctcccgctat ggctgcgctg cggaggttcc tgtggccgcc
       61 gcctcggctg tcgccggcgc tcgcccctca gcagcccttc ctctcaccgt gggggcggcc
      121 tgcggggacg gctcccggga tgtcgggccg gcccttctcc tgccgggagg aggacgaggg
      181 ggccgtcgcg gaggcggcgt ggcggcggcg acgctggggc gagctgagca tcgcggcggc
      241 ggctggcggg gggctggtcg gccttgtgtg ctaccagctg tatggggacc ccagagccga
      301 cccctcggag ctagcggccc cggaactcga ggacccgccc cgcggcaggg ggctgctgcc
      361 catcccggtg gctgctgcca aggagacggt tgctactggc agagccatta ctgaagactt
      421 ggacctctat gctacatctc gagagagacg gtttcgttta tttgcatcta tagaatgtga
      481 aggacagtta ttcatgactc catatgattt tattctggct gttacaacag atgagcccaa
      541 atttgctaaa acgtggaagt cactttctaa acaggaatta agtcagatgc tctcagaaac
      601 tccaccagtt tggaagggat catcaaagct ttttcgaaat cttaaagaaa gaggtgtgat
      661 ttcttacaca gaatatcttt ttcttttatg tattttaaca aagccccatg caggttttag
      721 aatagctttc aacatgtttg acactgacgg aaatgagatg gtggataaaa aagaattttt
      781 ggtgcttcag gagatattca ggaaaaaaaa tgaaaagaga gaaactaaag gagatgaaga
      841 aaaacgtgcc atgctgcgtc ttcaacttta tggataccat tctcctacta atagtgtact
      901 taaaacagat gctggggaac ttgtctccag aagctactgg gataccctga gacgtagcac
      961 aagccaggcg ctcttctcag acctcgcaga gcgtgctgat gacatcacaa gcttagtagc
     1021 agatactaca cttctggtac acttttttgg aaaaaaagga aaagctgagc tcaactttga
     1081 agatttttat agatttatgg acaatctaca aacagaagtt ctagaaatag aatttctttc
     1141 ctactctaat ggaatgaata ctatcagtga agaagatttt gcccatattc tcctgcggta
     1201 tactaatgtg gaaaatacat cagtattttt agaaaatgtg cgctacagta tatctgaaga
     1261 aaagggcatc acatttgatg agttcaggtc attcttccag tttctgaaca acttagaaga
     1321 ctttgcaata gctctcaaca tgtataactt tgcaagccgc tctataggac aagatgaatt
     1381 taaacgtgct gtatacgtag ctactggact gaaactttca ccacatttgg tgaacactgt
     1441 cttcaagatt tttgatgttg acaaagatga ccaattaagt tataaagaat ttattggaat
     1501 tatgaaagac agactccata gagggttccg gggttataaa acagtccaga agtatccgac
     1561 cttcaaatcc tgcctgaaaa aggaacttca cagcagataa atcgtcctaa acaagaattg
     1621 aaggattatg tcctatgtga ccaggcaagg agcaagattt tgagtggatc caaatgtaag
     1681 gtcagagcac gcagaaaatg aagggaacgt gaaatgctat gaaatttaca tttttttcct
     1741 tgaatggagt ttgtcagcgt ccaacaggaa aactgcatct tctaattaca agcattagct
     1801 ggcgttgagc ctgggcacca ccagagaaaa cttgctgcaa acactaaatc agccgagctt
     1861 tcagagcagt gaccggctgc tctgaacttc ttcattccct acacctcggt agtcccaatg
     1921 actgtctagt ctcatattta gattatttat ttattagaaa tgtcctttat ttcagaataa
     1981 tttatgaatg atagaatata gatggaaaca cattttcttc aggtttgcac tgtgagcgtg
     2041 ttttcataca gtgaatattg cttcagattt gtcctgggcc cttaaattgt tagtctctgt
     2101 tatttatctt gtatttaatt atactgaaga aatttcagaa aacaagtgaa aatttactaa
     2161 gcaataggat ggtttacatt gtactttcaa cttttaaaag tacttatgta atatatgttg
     2221 ttggatttat gtaaattttt gtttgttttt gctgttcttt gtgttttgtt tttgtaccaa
     2281 agccaacaga gattcagtta ttttatttta acagaaaatt agaatttttt tttttttttt
     2341 ttttttttta cgaaaacaca gcagtcatgt actgatgtta gtaaccacca ccctgggatt
     2401 tctagtcgca ggtgtgacat atggaactaa gaactgtaac atgggctatt taatatatgt
     2461 ctatagcgtc agaactaatg taaatggcct agaaggttga tgtatattta tttaatagat
     2521 aggagataat gttttgtttc ttgaaaaaaa ggaccacgtt caaaattgat ccaatcagtc
     2581 agtagtgatg aatcattggt atgtaatcca ttacatcaaa ataatgtgtc ttaaaagaga
     2641 aaataaaact ttctcttgaa aaccccactt gagagactgg ccagcatggg tcacctcaaa
     2701 gtctgcctgg ctgttgtgca aactcaagcc atcttggcaa cttagaagaa ccctgccaaa
     2761 gataaaaacc actgggaaag tacgtagctt agtgcagagg gtttgcctgc cgtgcatgag
     2821 gccttaggtt taattcccaa ctctgaaaaa gaaagggaag gcaagagagg ggaagggaag
     2881 ggaaagaaag aagtagaaaa agaaaaagag gaatcctaga tttttatgtt gaaaaataat
     2941 tttagaatat ttaatagaac tttggctacc tgataaaatt ggtttataca aaggtactat
     3001 aaaatactgc aatgagttta atacatctat gtattgcatg tcaaaagatt aatagattcg
     3061 tttatcttct tttgtaagaa gcacgtagta cacactgcat acactccaac cattactttg
     3121 cttttgcata ggaaaatagg aaacactggc tttaagttat tgaatgtgaa attgatctgt
     3181 atcagcctat ttgtaaactg ttattttggt gttattatta ttgattatgt tttctagacc
     3241 tttccacatt gttcatctga tattatttat tgcaccaata aagaaataga aatgtgcaaa
//
