LOCUS       NM_028643               2326 bp    mRNA    linear   ROD 14-OCT-2024
DEFINITION  Mus musculus mitochondrial calcium uptake 2 (Micu2), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_028643
VERSION     NM_028643.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Hasan,P., Berezhnaya,E., Rodriguez-Prados,M., Weaver,D.,
            Bekeova,C., Cartes-Saavedra,B., Birch,E., Beyer,A.M., Santos,J.H.,
            Seifert,E.L., Elrod,J.W. and Hajnoczky,G.
  TITLE     MICU1 and MICU2 control mitochondrial calcium signaling in the
            mammalian heart
  JOURNAL   Proc Natl Acad Sci U S A 121 (35), e2402491121 (2024)
   PUBMED   39163336
  REMARK    GeneRIF: MICU1 and MICU2 control mitochondrial calcium signaling in
            the mammalian heart.
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2326)
  AUTHORS   Singh,R., Bartok,A., Paillard,M., Tyburski,A., Elliott,M. and
            Hajnoczky,G.
  TITLE     Uncontrolled mitochondrial calcium uptake underlies the
            pathogenesis of neurodegeneration in MICU1-deficient mice and
            patients
  JOURNAL   Sci Adv 8 (11), eabj4716 (2022)
   PUBMED   35302860
REFERENCE   4  (bases 1 to 2326)
  AUTHORS   Vishnu,N., Hamilton,A., Bagge,A., Wernersson,A., Cowan,E.,
            Barnard,H., Sancak,Y., Kamer,K.J., Spegel,P., Fex,M., Tengholm,A.,
            Mootha,V.K., Nicholls,D.G. and Mulder,H.
  TITLE     Mitochondrial clearance of calcium facilitated by MICU2 controls
            insulin secretion
  JOURNAL   Mol Metab 51, 101239 (2021)
   PUBMED   33932586
  REMARK    GeneRIF: Mitochondrial clearance of calcium facilitated by MICU2
            controls insulin secretion.
REFERENCE   5  (bases 1 to 2326)
  AUTHORS   Kamer,K.J., Jiang,W., Kaushik,V.K., Mootha,V.K. and Grabarek,Z.
  TITLE     Crystal structure of MICU2 and comparison with MICU1 reveal
            insights into the uniporter gating mechanism
  JOURNAL   Proc Natl Acad Sci U S A 116 (9), 3546-3555 (2019)
   PUBMED   30755530
  REMARK    GeneRIF: s. We propose that in the MICU1-MICU2 oligomeric complex
            the C-terminal helices of both proteins form a central
            semiautonomous assembly which contributes to the gating mechanism
            of the uniporter.
REFERENCE   6  (bases 1 to 2326)
  AUTHORS   Bick,A.G., Wakimoto,H., Kamer,K.J., Sancak,Y., Goldberger,O.,
            Axelsson,A., DeLaughter,D.M., Gorham,J.M., Mootha,V.K.,
            Seidman,J.G. and Seidman,C.E.
  TITLE     Cardiovascular homeostasis dependence on MICU2, a regulatory
            subunit of the mitochondrial calcium uniporter
  JOURNAL   Proc Natl Acad Sci U S A 114 (43), E9096-E9104 (2017)
   PUBMED   29073106
  REMARK    GeneRIF: Micu2 role in regulating angiotensin II-mediated
            hypertensive responses that are critical in protecting the
            abdominal aorta from injury.
REFERENCE   7  (bases 1 to 2326)
  AUTHORS   Paillard,M., Csordas,G., Szanda,G., Golenar,T., Debattisti,V.,
            Bartok,A., Wang,N., Moffat,C., Seifert,E.L., Spat,A. and
            Hajnoczky,G.
  TITLE     Tissue-Specific Mitochondrial Decoding of Cytoplasmic Ca2+ Signals
            Is Controlled by the Stoichiometry of MICU1/2 and MCU
  JOURNAL   Cell Rep 18 (10), 2291-2300 (2017)
   PUBMED   28273446
  REMARK    GeneRIF: a possible role for tissue-specific stoichiometry between
            the Ca(2+)-sensing regulators (MICUs) and pore unit (MCU) of the
            uniporter, was investigated.
REFERENCE   8  (bases 1 to 2326)
  AUTHORS   Patron,M., Checchetto,V., Raffaello,A., Teardo,E., Vecellio
            Reane,D., Mantoan,M., Granatiero,V., Szabo,I., De Stefani,D. and
            Rizzuto,R.
  TITLE     MICU1 and MICU2 finely tune the mitochondrial Ca2+ uniporter by
            exerting opposite effects on MCU activity
  JOURNAL   Mol Cell 53 (5), 726-737 (2014)
   PUBMED   24560927
REFERENCE   9  (bases 1 to 2326)
  AUTHORS   Plovanich,M., Bogorad,R.L., Sancak,Y., Kamer,K.J., Strittmatter,L.,
            Li,A.A., Girgis,H.S., Kuchimanchi,S., De Groot,J., Speciner,L.,
            Taneja,N., Oshea,J., Koteliansky,V. and Mootha,V.K.
  TITLE     MICU2, a paralog of MICU1, resides within the mitochondrial
            uniporter complex to regulate calcium handling
  JOURNAL   PLoS One 8 (2), e55785 (2013)
   PUBMED   23409044
REFERENCE   10 (bases 1 to 2326)
  AUTHORS   Chang,S.Y., Yong,T.F., Yu,C.Y., Liang,M.C., Pletnikova,O.,
            Troncoso,J., Burgunder,J.M. and Soong,T.W.
  TITLE     Age and gender-dependent alternative splicing of P/Q-type calcium
            channel EF-hand
  JOURNAL   Neuroscience 145 (3), 1026-1036 (2007)
   PUBMED   17291689
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125399.3.
            
            On Mar 21, 2023 this sequence version replaced NM_028643.3.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031172.1, AK167296.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-230               AC125399.3         106930-107159       c
            231-369             AC125399.3         79551-79689         c
            370-401             AC125399.3         62899-62930         c
            402-477             AC125399.3         62036-62111         c
            478-525             AC125399.3         53285-53332         c
            526-608             AC125399.3         51488-51570         c
            609-674             AC125399.3         40138-40203         c
            675-772             AC125399.3         35097-35194         c
            773-944             AC125399.3         31840-32011         c
            945-1053            AC125399.3         27244-27352         c
            1054-1211           AC125399.3         26723-26880         c
            1212-2326           AC125399.3         24161-25275         c
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 30.51 cM"
     gene            1..2326
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="mitochondrial calcium uptake 2"
                     /db_xref="GeneID:68514"
                     /db_xref="MGI:MGI:1915764"
     exon            1..230
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             21..1319
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     EF-hand domain-containing family member A1; EF hand domain
                     family A1; calcium uptake protein 2, mitochondrial"
                     /codon_start=1
                     /product="calcium uptake protein 2, mitochondrial isoform
                     1"
                     /protein_id="NP_082919.1"
                     /db_xref="CCDS:CCDS27163.1"
                     /db_xref="GeneID:68514"
                     /db_xref="MGI:MGI:1915764"
                     /translation="MAAAAGRSAWLAAWGGRLRRGLAAGRRAVPTRGPLAAAVAGVAL
                     AGAGAAWHHGRVKAAAREGSRTVSAQKNYLGPIEKLSLRKQRFMQFSSLEHDGEYYMT
                     PRDFLFSVMFEQVERKTLVKKLAKKDIEDVLSGIQTARCGSTFFRDLGDKGVISYTEY
                     LFLLTILTKPHSGFHVAFKMLDVDGNEMIERKEFVKLQKIISKQDGFKTVKTNETEYQ
                     DPTVKEPGVNTTLQVRFFGKRGEKKLHYKEFRRFMENLQTEVQEMEFLQFSKGLNFMR
                     KEDFAEWLLFFTNTENKDIYWRNVREKLSVGESISLDEFKSFCHFTTHLEDFAIAMQM
                     FSLAHRPVRLAEFKRAVKVATGQELSDNLLDTVFKIFDLDGDECLSHGEFLGVLKNRM
                     HRGLWVSQQQSVQEYWKCVKKESIKGVKEAWRQQAGKGPF"
     exon            231..369
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            370..401
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            402..477
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            478..525
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..608
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            609..674
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            675..772
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            773..944
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            945..1053
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1211
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..2326
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1662..1667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="hexamer: AATACA"
     polyA_site      1682
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="major polyA site"
     regulatory      2282..2287
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="hexamer: AATAAA"
     polyA_site      2326
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /note="major polyA site"
ORIGIN      
        1 gctcgggcgc ttccgcaaag atggcggcgg ctgcgggaag aagcgcttgg ctggcggcct
       61 ggggcggaag gctgcggcgc gggctcgcgg ccggccgacg agctgtgccg actcgcggcc
      121 ctctcgcagc ggctgtggcc ggggtagccc tggcaggcgc aggagcggca tggcatcacg
      181 gccgcgtgaa agcagccgcg cgcgagggct cccgaacggt gtcggcgcag aaaaattatt
      241 tgggaccaat agagaaactt tctctccgca aacagcggtt catgcagttt tcatccctgg
      301 agcacgacgg agagtattac atgacacccc gagacttcct cttctcagtc atgtttgagc
      361 aagtggagcg taaaacactg gtcaagaagc tggcaaaaaa ggatatcgag gatgtactgt
      421 caggaatcca aacagcacgc tgtggatcaa cattttttag agacctgggt gataaagggg
      481 taatttcata taccgagtat cttttcttgc ttacaatcct cacaaaacct cactctgggt
      541 tccatgttgc ttttaaaatg ctggatgtgg atgggaatga gatgatcgag aggaaggagt
      601 ttgttaagct gcagaagatc ataagtaaac aagatggctt caagacagtg aagactaatg
      661 agacagaata ccaggaccca acagtgaaag agcctggcgt taacacaacc cttcaagtgc
      721 gtttctttgg gaaaagagga gaaaaaaaac tccattataa agaatttcga agatttatgg
      781 aaaatttaca aacagaagtt caagaaatgg aattccttca gttctctaaa ggcctgaatt
      841 tcatgagaaa agaagacttt gcagaatggc tacttttctt cactaacact gaaaataaag
      901 atatttactg gagaaacgtg agagaaaagt tgtcagtagg agagagcatt agtttggatg
      961 agttcaagtc cttttgccat tttacaacgc atttggaaga ctttgctatc gccatgcaaa
     1021 tgtttagttt agctcatcgc cctgtgcggc tggcagagtt caagagagct gtgaaggtag
     1081 cgactggaca ggagctctcg gacaatcttc tggacaccgt cttcaagatc tttgacctgg
     1141 acggggacga gtgcctcagc cacggcgagt tccttggggt actgaaaaac aggatgcacc
     1201 gaggcttatg ggtgtcacag cagcagagtg tgcaagaata ctggaagtgt gtgaagaagg
     1261 aaagcatcaa gggagtaaag gaagcctgga gacaacaagc cggcaagggc cccttctaag
     1321 gggaagctga tggagctcca ggagccagaa agtggttttt ccaagagctg actgtttcta
     1381 tgtcttcatt gagtttcttt tgagtgtgaa gatgtgaaga ctcactttct gatttggggg
     1441 atttgggagg gatgacggga ggtgaataag tgtgtgccac agtgggaagt cagaggccag
     1501 cctgtaggag ctggttctct ctgtctacca tgggagtccc agagattgaa ctcaggtctc
     1561 gagctttgac atcaagtgcc tttacctgat gagccatctc accagctccg actccatcat
     1621 ttcttggtga attttcatta cacaaagtat tcctttatga aaatacacac tgctctatta
     1681 aagacttgtg tacaaagtgt gctttctgga gggctaaaca agtcagggaa ggaacttcat
     1741 tcccaagaca ggtactgatc ccagaagccg tgatagctta gggaatactt gcagttaaag
     1801 gcttgctcca gtgttctttg ctccctgcct tgaaatagca cataatggca gtgtccggtc
     1861 tggcagcagg atgaagtatt tacattgata caaaaaaatt ttttcttttt taaatgttcc
     1921 atgcttttga aatataaata gagtgtcctc tcccagacac tcccagggga gctcagccag
     1981 caaaggacac agctgtgtac catgctttcc taacaagcag cacacagagt tccatctaaa
     2041 accactcata cacacacaga tcacacacag atcacacaca tacagtgatc ataatagcaa
     2101 gttttcttaa gctacgggat cttgggttgt gggaaatatg gcactataaa gtagcttgca
     2161 ataaaataaa taggaaataa tgttatgtgg agatggacat gtgtatgtat acaggtggac
     2221 atacatgtgt gtgtggaagc ccctgctcct gtaaataccc ccatgtttct gaagtgatcc
     2281 caataaaccc atgagctcac caagctagac ttgggtgata ttgcta
//
