LOCUS       NP_082919                432 aa            linear   ROD 05-MAY-2025
DEFINITION  calcium uptake protein 2, mitochondrial isoform 1 [Mus musculus].
ACCESSION   NP_082919
VERSION     NP_082919.1
DBSOURCE    REFSEQ: accession NM_028643.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 432)
  AUTHORS   Hasan,P., Berezhnaya,E., Rodriguez-Prados,M., Weaver,D.,
            Bekeova,C., Cartes-Saavedra,B., Birch,E., Beyer,A.M., Santos,J.H.,
            Seifert,E.L., Elrod,J.W. and Hajnoczky,G.
  TITLE     MICU1 and MICU2 control mitochondrial calcium signaling in the
            mammalian heart
  JOURNAL   Proc Natl Acad Sci U S A 121 (35), e2402491121 (2024)
   PUBMED   39163336
  REMARK    GeneRIF: MICU1 and MICU2 control mitochondrial calcium signaling in
            the mammalian heart.
REFERENCE   2  (residues 1 to 432)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 432)
  AUTHORS   Singh,R., Bartok,A., Paillard,M., Tyburski,A., Elliott,M. and
            Hajnoczky,G.
  TITLE     Uncontrolled mitochondrial calcium uptake underlies the
            pathogenesis of neurodegeneration in MICU1-deficient mice and
            patients
  JOURNAL   Sci Adv 8 (11), eabj4716 (2022)
   PUBMED   35302860
REFERENCE   4  (residues 1 to 432)
  AUTHORS   Vishnu,N., Hamilton,A., Bagge,A., Wernersson,A., Cowan,E.,
            Barnard,H., Sancak,Y., Kamer,K.J., Spegel,P., Fex,M., Tengholm,A.,
            Mootha,V.K., Nicholls,D.G. and Mulder,H.
  TITLE     Mitochondrial clearance of calcium facilitated by MICU2 controls
            insulin secretion
  JOURNAL   Mol Metab 51, 101239 (2021)
   PUBMED   33932586
  REMARK    GeneRIF: Mitochondrial clearance of calcium facilitated by MICU2
            controls insulin secretion.
REFERENCE   5  (residues 1 to 432)
  AUTHORS   Kamer,K.J., Jiang,W., Kaushik,V.K., Mootha,V.K. and Grabarek,Z.
  TITLE     Crystal structure of MICU2 and comparison with MICU1 reveal
            insights into the uniporter gating mechanism
  JOURNAL   Proc Natl Acad Sci U S A 116 (9), 3546-3555 (2019)
   PUBMED   30755530
  REMARK    GeneRIF: s. We propose that in the MICU1-MICU2 oligomeric complex
            the C-terminal helices of both proteins form a central
            semiautonomous assembly which contributes to the gating mechanism
            of the uniporter.
REFERENCE   6  (residues 1 to 432)
  AUTHORS   Bick,A.G., Wakimoto,H., Kamer,K.J., Sancak,Y., Goldberger,O.,
            Axelsson,A., DeLaughter,D.M., Gorham,J.M., Mootha,V.K.,
            Seidman,J.G. and Seidman,C.E.
  TITLE     Cardiovascular homeostasis dependence on MICU2, a regulatory
            subunit of the mitochondrial calcium uniporter
  JOURNAL   Proc Natl Acad Sci U S A 114 (43), E9096-E9104 (2017)
   PUBMED   29073106
  REMARK    GeneRIF: Micu2 role in regulating angiotensin II-mediated
            hypertensive responses that are critical in protecting the
            abdominal aorta from injury.
REFERENCE   7  (residues 1 to 432)
  AUTHORS   Paillard,M., Csordas,G., Szanda,G., Golenar,T., Debattisti,V.,
            Bartok,A., Wang,N., Moffat,C., Seifert,E.L., Spat,A. and
            Hajnoczky,G.
  TITLE     Tissue-Specific Mitochondrial Decoding of Cytoplasmic Ca2+ Signals
            Is Controlled by the Stoichiometry of MICU1/2 and MCU
  JOURNAL   Cell Rep 18 (10), 2291-2300 (2017)
   PUBMED   28273446
  REMARK    GeneRIF: a possible role for tissue-specific stoichiometry between
            the Ca(2+)-sensing regulators (MICUs) and pore unit (MCU) of the
            uniporter, was investigated.
REFERENCE   8  (residues 1 to 432)
  AUTHORS   Patron,M., Checchetto,V., Raffaello,A., Teardo,E., Vecellio
            Reane,D., Mantoan,M., Granatiero,V., Szabo,I., De Stefani,D. and
            Rizzuto,R.
  TITLE     MICU1 and MICU2 finely tune the mitochondrial Ca2+ uniporter by
            exerting opposite effects on MCU activity
  JOURNAL   Mol Cell 53 (5), 726-737 (2014)
   PUBMED   24560927
REFERENCE   9  (residues 1 to 432)
  AUTHORS   Plovanich,M., Bogorad,R.L., Sancak,Y., Kamer,K.J., Strittmatter,L.,
            Li,A.A., Girgis,H.S., Kuchimanchi,S., De Groot,J., Speciner,L.,
            Taneja,N., Oshea,J., Koteliansky,V. and Mootha,V.K.
  TITLE     MICU2, a paralog of MICU1, resides within the mitochondrial
            uniporter complex to regulate calcium handling
  JOURNAL   PLoS One 8 (2), e55785 (2013)
   PUBMED   23409044
REFERENCE   10 (residues 1 to 432)
  AUTHORS   Chang,S.Y., Yong,T.F., Yu,C.Y., Liang,M.C., Pletnikova,O.,
            Troncoso,J., Burgunder,J.M. and Soong,T.W.
  TITLE     Age and gender-dependent alternative splicing of P/Q-type calcium
            channel EF-hand
  JOURNAL   Neuroscience 145 (3), 1026-1036 (2007)
   PUBMED   17291689
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125399.3.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031172.1, AK167296.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 30.51 cM"
     Protein         1..432
                     /product="calcium uptake protein 2, mitochondrial isoform
                     1"
                     /note="EF-hand domain-containing family member A1; EF hand
                     domain family A1; calcium uptake protein 2, mitochondrial"
                     /calculated_mol_wt=47195
     transit_peptide 1..22
                     /note="Mitochondrion. /evidence=ECO:0000255; propagated
                     from UniProtKB/Swiss-Prot (Q8CD10.2)"
                     /calculated_mol_wt=2299
     mat_peptide     23..432
                     /product="Calcium uptake protein 2, mitochondrial.
                     /id=PRO_0000251218"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8CD10.2)"
                     /calculated_mol_wt=47195
     Region          81..199
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          173..392
                     /region_name="EFh_MICU2"
                     /note="EF-hand, calcium binding motif, found in calcium
                     uptake protein 2, mitochondrial (MICU2) and similar
                     proteins; cd16174"
                     /db_xref="CDD:320082"
     Region          173..202
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320082"
     Site            order(182,184,186,193,372,374,376,383)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320082"
     Site            202
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8IYU8; propagated from
                     UniProtKB/Swiss-Prot (Q8CD10.2)"
     Region          230..260
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320082"
     Region          326..355
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320082"
     Region          363..392
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320082"
     CDS             1..432
                     /gene="Micu2"
                     /gene_synonym="1110008L20Rik; 4833427E09Rik; Efha1; Smhs2"
                     /coded_by="NM_028643.4:21..1319"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS27163.1"
                     /db_xref="GeneID:68514"
                     /db_xref="MGI:MGI:1915764"
ORIGIN      
        1 maaaagrsaw laawggrlrr glaagrravp trgplaaava gvalagagaa whhgrvkaaa
       61 regsrtvsaq knylgpiekl slrkqrfmqf sslehdgeyy mtprdflfsv mfeqverktl
      121 vkklakkdie dvlsgiqtar cgstffrdlg dkgvisytey lflltiltkp hsgfhvafkm
      181 ldvdgnemie rkefvklqki iskqdgfktv ktneteyqdp tvkepgvntt lqvrffgkrg
      241 ekklhykefr rfmenlqtev qemeflqfsk glnfmrkedf aewllfftnt enkdiywrnv
      301 reklsvgesi sldefksfch ftthledfai amqmfslahr pvrlaefkra vkvatgqels
      361 dnlldtvfki fdldgdecls hgeflgvlkn rmhrglwvsq qqsvqeywkc vkkesikgvk
      421 eawrqqagkg pf
//
