LOCUS       NM_174850               3346 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus MICAL-like 2 (Micall2), mRNA.
ACCESSION   NM_174850
VERSION     NM_174850.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Sakane,A., Yano,T.A., Uchihashi,T., Horikawa,K., Hara,Y., Imoto,I.,
            Kurisu,S., Yamada,H., Takei,K. and Sasaki,T.
  TITLE     JRAB/MICAL-L2 undergoes liquid-liquid phase separation to form
            tubular recycling endosomes
  JOURNAL   Commun Biol 4 (1), 551 (2021)
   PUBMED   33976349
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Miyake,K., Sakane,A., Tsuchiya,Y., Sagawa,I., Tomida,Y.,
            Kasahara,J., Imoto,I., Watanabe,S., Higo,D., Mizuguchi,K. and
            Sasaki,T.
  TITLE     Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is
            Fine-tuned by Intramolecular Interaction between First LIM Zinc
            Finger and C-terminal Coiled-coil Domains
  JOURNAL   Sci Rep 9 (1), 12794 (2019)
   PUBMED   31488862
  REMARK    GeneRIF: the LIM domain, independent of the C-terminus, is
            necessary for the function of open-form JRAB/MICAL-L2
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3346)
  AUTHORS   Nakajo,A., Yoshimura,S., Togawa,H., Kunii,M., Iwano,T., Izumi,A.,
            Noguchi,Y., Watanabe,A., Goto,A., Sato,T. and Harada,A.
  TITLE     EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed
            transport in polarized epithelial cells
  JOURNAL   J Cell Biol 212 (3), 297-306 (2016)
   PUBMED   26833786
REFERENCE   4  (bases 1 to 3346)
  AUTHORS   Sakane,A., Alamir Mahmoud Abdallah,A., Nakano,K., Honda,K.,
            Kitamura,T., Imoto,I., Matsushita,N. and Sasaki,T.
  TITLE     Junctional Rab13-binding protein (JRAB) regulates cell spreading
            via filamins
  JOURNAL   Genes Cells 18 (9), 810-822 (2013)
   PUBMED   23890175
  REMARK    GeneRIF: Interaction between JRAB and filamin regulates
            JRABDeltaCC-induced cell spreading.
REFERENCE   5  (bases 1 to 3346)
  AUTHORS   Sakane,A., Abdallah,A.A., Nakano,K., Honda,K., Ikeda,W.,
            Nishikawa,Y., Matsumoto,M., Matsushita,N., Kitamura,T. and
            Sasaki,T.
  TITLE     Rab13 small G protein and junctional Rab13-binding protein (JRAB)
            orchestrate actin cytoskeletal organization during epithelial
            junctional development
  JOURNAL   J Biol Chem 287 (51), 42455-42468 (2012)
   PUBMED   23100251
  REMARK    GeneRIF: Rab13 and JRAB regulate reorganization of the actin
            cytoskeleton throughout epithelial junctional development.
REFERENCE   6  (bases 1 to 3346)
  AUTHORS   Sakane,A., Honda,K. and Sasaki,T.
  TITLE     Rab13 regulates neurite outgrowth in PC12 cells through its
            effector protein, JRAB/MICAL-L2
  JOURNAL   Mol Cell Biol 30 (4), 1077-1087 (2010)
   PUBMED   20008558
  REMARK    GeneRIF: show that Rab13 regulates neurite outgrowth in the rat
            pheochromocytoma cell line PC12 through an interaction with
            JRAB/MICAL-L2.
REFERENCE   7  (bases 1 to 3346)
  AUTHORS   Nakatsuji,H., Nishimura,N., Yamamura,R., Kanayama,H.O. and
            Sasaki,T.
  TITLE     Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to
            cell-cell junctions and the formation of functional tight junctions
  JOURNAL   Mol Cell Biol 28 (10), 3324-3335 (2008)
   PUBMED   18332111
  REMARK    GeneRIF: Although actinin-4 bound to JRAB/MICAL-L2 without Rab13,
            the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13
            activation.
REFERENCE   8  (bases 1 to 3346)
  AUTHORS   Yamamura,R., Nishimura,N., Nakatsuji,H., Arase,S. and Sasaki,T.
  TITLE     The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates
            the assembly of tight junctions and adherens junctions
  JOURNAL   Mol Biol Cell 19 (3), 971-983 (2008)
   PUBMED   18094055
  REMARK    GeneRIF: These results suggest that the interaction of
            JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight
            junctions and adherens junctions.
REFERENCE   9  (bases 1 to 3346)
  AUTHORS   Terai,T., Nishimura,N., Kanda,I., Yasui,N. and Sasaki,T.
  TITLE     JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the
            endocytic recycling of occludin
  JOURNAL   Mol Biol Cell 17 (5), 2465-2475 (2006)
   PUBMED   16525024
  REMARK    GeneRIF: JRAB/MICAL-L2 mediates the endocytic recycling of occludin
            and the formation of functional TJs by linking Rab13 to actin
            cytoskeleton.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY346995.1, AB182579.1 and BY700282.1.
            
            On Jul 22, 2009 this sequence version replaced NM_174850.2.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus and to correct mismatches versus the reference genome
            assembly in the coding region which results in 4 aa changes to the
            C-terminus of the protein.  This N-terminal extension is 114aa
            long, is supported by available conservation data and adds a CH
            (calponin homology) domain (SMART).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK151086.1, AK164043.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               BY346995.1         3-151
            150-3229            AB182579.1         1-3080
            3230-3346           BY700282.1         272-388
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 78.79 cM"
     gene            1..3346
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /note="MICAL-like 2"
                     /db_xref="GeneID:231830"
                     /db_xref="MGI:MGI:2444818"
     exon            1..316
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..3203
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /note="junctional Rab13-binding protein; molecule
                     interacting with CasL-like 2"
                     /codon_start=1
                     /product="MICAL-like protein 2"
                     /protein_id="NP_777275.2"
                     /db_xref="CCDS:CCDS39353.2"
                     /db_xref="GeneID:231830"
                     /db_xref="MGI:MGI:2444818"
                     /translation="MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHR
                     PDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQY
                     YNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPV
                     VQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQC
                     SSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTV
                     QEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVT
                     TTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGV
                     KTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTV
                     VTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNS
                     TNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQ
                     ESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALT
                     TQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPG
                     PISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSV
                     HITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDG
                     TRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQ
                     LDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLE
                     EQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQ
                     EEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA"
     exon            317..365
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            366..507
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            508..698
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            699..814
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            815..1954
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2211
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2212..2305
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2306..2463
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2613
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2614..2678
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2679..2802
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2803..2893
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2967
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3082
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            3083..3129
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3346
                     /gene="Micall2"
                     /gene_synonym="A930021H16Rik; JRAB; mFLJ00139; MICAL-L2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggggcgggg ccggcggcct cgcaggcgtc aagagtgtgt ggggcgccgg tctcccggtg
       61 ttctcttctt ccagcccggc tggtggtcgg cgggtccggg cctcctgcgc gtctcgggcc
      121 caagaggggc ctgtagccag ctcctcgagc cgccagcccg tcctgccgcc catatggccg
      181 ccataaaggc cctgcaagaa tggtgccggc agcagtgcga gggctaccgg gacgtgagca
      241 tcaccaacat gaccacctcg ttccgcgatg gcctggcctt ctgcgctatc ttgcaccgac
      301 accggcctga ccttataaac ttcagtgccc tccggaagga aaacatttac gagaacaaca
      361 agttggcctt ccaagtggct gaggaacagc tgggcatccc agccctgcta gacgctgagg
      421 acatggtggc cttgaaggtt ccagaccggc tgagcattct cacctatgtg tcacaatact
      481 acaactactt ccatggccgg tccccaattg ggggcatggc tggcataaag agaccctcat
      541 ccgattcgac cgaagaactt tctggaaaga agggcctgtc ccagcctgcc aagttgccct
      601 cgccggctca aacccagagg tcaccactgt ccccagctcg gacaaaccct gtggttcaga
      661 ggaatgaagg tggctcacaa agaccatccc caaaggctgc tccggggacc gcgggaagct
      721 ctgtcagcag tatctgtggg gtctgtggca aacatgttca ccttgtgcag cggcacctgg
      781 ctgacgggcg cctctaccac cgaagctgct tcaggtgtaa acagtgctcc agcacgctgc
      841 actcgggggc ctaccgtgcc acgggagagc ccggagtctt cgtctgtacc caccacagct
      901 cagaagtcac ctctgtgagc cccaagtcat caaacctggc ctccaggaaa ccagggggtg
      961 tcactgcaga caccaggcca ttcggcgtct catggacggt tcaggaggca aatggagagg
     1021 ggacaccact gagggtcagg acagcagcat gggaacacgc tggaggaaac acaaccgcca
     1081 aaggttttgt ccagacggaa cttaagccac catctacctc ccaagtccac gtggggagct
     1141 ctgcggggcc caagctaccc acgatcacag tgactactac ttctgtgact agcaaagctc
     1201 tcacccatgt gacgaatagc tccccaatag gatggtcatc gcctgcccag agcagcccag
     1261 cgaacttcaa ctcccgccca gttgtatccc caagtgctcg gaacactcac ctacctgggt
     1321 cccaagggca gacagcctca aaaggtgtta agactcagct caacttgaac tcagagtctt
     1381 caaacacagc ggtcacccca gcctggactt cctcagcctc taagacccag caggcccgag
     1441 agaaattttt ccagacccct ccctcggccc cagccccagc ctcagcccca gccccagccc
     1501 ccaccagcaa ggtccccact gtagtgactg tccccaccag caaggtcccc aatgtggtga
     1561 ctgcccccac cagcaaggtc cccactgtag tgactgtccc caccagcaag gtccccactg
     1621 tggtgtctgc ccccaccagc aaggtcccta ctgtggtgtc tgcccccacc agcaaggtcc
     1681 ccactgtggt gaattccact aatagcaggg tcaccacggt agtaaatgcc cccaccagca
     1741 aggtccccac tgtagtgagt gccacaaatg gcagggtgcc cactgtggtg actgcccaca
     1801 ctggtagggt ccctgctgtg atgaatacct ctgccagcaa agtctctcct gttgtggatg
     1861 cccctgccca ggagagcagc cgggagcaag cattaagtgt ccttaggaag gcccttccgg
     1921 cgctgacagg atctggtacc caggctccaa acaggtcctt cccagccact tcctctgtcc
     1981 tggtaaccct tcccaagaat gaagtgccac aaaaagttcc ctcagacaag ctctcagctt
     2041 taaccaccca gactcctaat ttcactataa agctagagcc ttcagcccca gtgaatgtgg
     2101 gcaataccgc tgtatttctc caggctggca aaaagagccc gagtatttct ccaagggttg
     2161 gtaagaccag cgttggctcc aggccacaag cagaggtggc aggagtaaag ggtccaggtc
     2221 ccatctctca ggaaggccaa gaggagggac cagaaggatg gagggcccgg ctgaagcctg
     2281 tggataagaa aacccctgct gggaggtctc tggagcaaaa agagccagtc ctggcagagc
     2341 caaggatagg tgacacttcc aggaaagcct ctagtagctc tgactccagc gtccatatca
     2401 ccttgacttc tattcagcac aagaggaaac catgccctgc tggttccggg cccagtcctg
     2461 cagccctctc tccttctcca tcacaccgca agaaactagc tgtccctccc agccttgatg
     2521 tttcagctga ttggctccag ccagagccca agaagcagga agatgggacc aggagctgta
     2581 aggaggagaa gtcacccacc aggtggtcaa gagagaggtc tgcagtcctg gacagtggcc
     2641 ttgctccacc tggtgaagct gtcacttccc cagtcaggct ccaccccgac tatatccccc
     2701 aggaggagct gcagaggcag ctgcaggaca ttgagagcca actggacgcc ctggagctca
     2761 ggggtgttga gttggagaag cggctgcggg cagctgaggg agatgcttct gaggatagcc
     2821 tcatggtgga ctggttccgg cttattcatg agaagcagct actgttgagg ctggaatcgg
     2881 aactcatgta caagtccaag gaccagcgcc tggaggagca gcagctggac ctccagggtg
     2941 agctgcgcag gctgatggac aagccagagg gtctgaagtc cccccaggac cgccagcggg
     3001 agcaggagct gctgagccaa tacgtgaaca ccgtgaatga ccgaagtgac atcgttgact
     3061 tcctggatga ggatcggctc cgggaacagg aagaggacca aatgctggag aatatgatcc
     3121 agaacctggg cctccagaga aagaagtcca agtccttctt gtccaagatc tggtcctcga
     3181 agagcaaaag cgggcaggcc tgagctgcac gtggctggca ctgggccctg gacctcctca
     3241 ggaagagact ggtgctgcgg tccccgaaag ggatactggg ggtgccaggg cctgggcctc
     3301 ccactctggg tcctcgtcac ctcaataaag acactgacct tcccac
//
