LOCUS       NM_144860              10154 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus MIB E3 ubiquitin protein ligase 1 (Mib1), transcript
            variant 1, mRNA.
ACCESSION   NM_144860 XM_976932
VERSION     NM_144860.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Siguero-Alvarez,M., Salguero-Jimenez,A., Grego-Bessa,J., de la
            Barrera,J., MacGrogan,D., Prados,B., Sanchez-Saez,F.,
            Pineiro-Sabaris,R., Felipe-Medina,N., Torroja,C., Gomez,M.J.,
            Sabater-Molina,M., Escriba,R., Richaud-Patin,I.,
            Iglesias-Garcia,O., Sbroggio,M., Callejas,S., O'Regan,D.P.,
            McGurk,K.A., Dopazo,A., Giovinazzo,G., Ibanez,B., Monserrat,L.,
            Perez-Pomares,J.M., Sanchez-Cabo,F., Pendas,A.M., Raya,A.,
            Gimeno-Blanes,J.R. and de la Pompa,J.L.
  TITLE     A Human Hereditary Cardiomyopathy Shares a Genetic Substrate With
            Bicuspid Aortic Valve
  JOURNAL   Circulation 147 (1), 47-65 (2023)
   PUBMED   36325906
  REMARK    Erratum:[Circulation. 2023 Jan 17;147(3):e63. doi:
            10.1161/CIR.0000000000001129.. PMID: 36649398]
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Huang,X., Zhao,K., Jiang,M., Qiu,D., Zhou,J. and Yang,Z.
  TITLE     The G4 resolvase RHAU regulates ventricular trabeculation and
            compaction through transcriptional and post-transcriptional
            mechanisms
  JOURNAL   J Biol Chem 298 (1), 101449 (2022)
   PUBMED   34838591
REFERENCE   3  (bases 1 to 10154)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10154)
  AUTHORS   Ofek,S., Wiszniak,S., Kagan,S., Tondl,M., Schwarz,Q. and
            Kalcheim,C.
  TITLE     Notch signaling is a critical initiator of roof plate formation as
            revealed by the use of RNA profiling of the dorsal neural tube
  JOURNAL   BMC Biol 19 (1), 84 (2021)
   PUBMED   33892704
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 10154)
  AUTHORS   Seo,J.Y., Kang,J.S., Kim,Y.L., Jo,Y.W., Kim,J.H., Hann,S.H.,
            Park,J., Park,I., Park,H., Yoo,K., Rhee,J., Park,J.W., Ha,Y.C. and
            Kong,Y.Y.
  TITLE     Maintenance of type 2 glycolytic myofibers with age by Mib1-Actn3
            axis
  JOURNAL   Nat Commun 12 (1), 1294 (2021)
   PUBMED   33637766
  REMARK    GeneRIF: Maintenance of type 2 glycolytic myofibers with age by
            Mib1-Actn3 axis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10154)
  AUTHORS   Barsi,J.C., Rajendra,R., Wu,J.I. and Artzt,K.
  TITLE     Mind bomb1 is a ubiquitin ligase essential for mouse embryonic
            development and Notch signaling
  JOURNAL   Mech Dev 122 (10), 1106-1117 (2005)
   PUBMED   16061358
  REMARK    GeneRIF: A targeted mutation of Mib1 in mice results in embryonic
            lethality;MIB1 activity is required for S3 cleavage of the Notch1
            receptor.
REFERENCE   7  (bases 1 to 10154)
  AUTHORS   Koo,B.K., Lim,H.S., Song,R., Yoon,M.J., Yoon,K.J., Moon,J.S.,
            Kim,Y.W., Kwon,M.C., Yoo,K.W., Kong,M.P., Lee,J., Chitnis,A.B.,
            Kim,C.H. and Kong,Y.Y.
  TITLE     Mind bomb 1 is essential for generating functional Notch ligands to
            activate Notch
  JOURNAL   Development 132 (15), 3459-3470 (2005)
   PUBMED   16000382
  REMARK    GeneRIF: mice lacking Mib1 die prior to embryonic day 11.5, with
            pan-Notch defects in somitogenesis, neurogenesis, vasculogenesis
            and cardiogenesis.
REFERENCE   8  (bases 1 to 10154)
  AUTHORS   Koo,B.K., Yoon,K.J., Yoo,K.W., Lim,H.S., Song,R., So,J.H., Kim,C.H.
            and Kong,Y.Y.
  TITLE     Mind bomb-2 is an E3 ligase for Notch ligand
  JOURNAL   J Biol Chem 280 (23), 22335-22342 (2005)
   PUBMED   15824097
REFERENCE   9  (bases 1 to 10154)
  AUTHORS   Itoh,M., Kim,C.H., Palardy,G., Oda,T., Jiang,Y.J., Maust,D.,
            Yeo,S.Y., Lorick,K., Wright,G.J., Ariza-McNaughton,L.,
            Weissman,A.M., Lewis,J., Chandrasekharappa,S.C. and Chitnis,A.B.
  TITLE     Mind bomb is a ubiquitin ligase that is essential for efficient
            activation of Notch signaling by Delta
  JOURNAL   Dev Cell 4 (1), 67-82 (2003)
   PUBMED   12530964
REFERENCE   10 (bases 1 to 10154)
  AUTHORS   Jin,Y., Blue,E.K., Dixon,S., Shao,Z. and Gallagher,P.J.
  TITLE     A death-associated protein kinase (DAPK)-interacting protein,
            DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis
            factor-induced apoptosis and regulates the cellular levels of DAPK
  JOURNAL   J Biol Chem 277 (49), 46980-46986 (2002)
   PUBMED   12351649
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY245539.1 and AC140316.3.
            
            On Jul 20, 2018 this sequence version replaced NM_144860.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY245539.1, AY147848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3793              AY245539.1         1-3793
            3794-10154          AC140316.3         122225-128585       c
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 5.35 cM"
     gene            1..10154
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="MIB E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:225164"
                     /db_xref="MGI:MGI:2443157"
     exon            1..909
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     CDS             681..3701
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /EC_number="2.3.2.27"
                     /note="DAPK-interacting protein-1; mind bomb homolog 1;
                     skeletrophin; mindbomb homolog 1; RING-type E3 ubiquitin
                     transferase MIB1; mindbomb E3 ubiquitin protein ligase 1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MIB1"
                     /protein_id="NP_659109.2"
                     /db_xref="CCDS:CCDS29058.1"
                     /db_xref="GeneID:225164"
                     /db_xref="MGI:MGI:2443157"
                     /translation="MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFES
                     PEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAE
                     CTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRG
                     VDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCV
                     QDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMF
                     ETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQ
                     VGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTY
                     NPAAVSKVAPAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDL
                     LKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGD
                     EGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTP
                     LHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWI
                     VDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIV
                     RLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIM
                     GLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVS
                     GQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQ
                     VQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFITCC
                     GGKSSEDPSDEISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKN
                     MIFLCGHGTCQLCGDRMSECPICRKAIERRILLY"
     exon            910..1081
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1211
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1316
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1383
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1588
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1772
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1773..1917
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2051
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2052..2159
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2357
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2509
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2642
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2729
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2891
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            2892..3073
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            3074..3266
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3345
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3459
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3560
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     exon            3561..10154
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9287..9292
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="hexamer: AATAAA"
     regulatory      9310..9315
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="hexamer: AATAAA"
     polyA_site      9311
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="major polyA site"
     polyA_site      9343
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
     regulatory      10129..10134
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="hexamer: AATAAT"
     regulatory      10132..10137
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
                     /note="hexamer: AATAAA"
     polyA_site      10147
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
     polyA_site      10154
                     /gene="Mib1"
                     /gene_synonym="DIP-1; E430019M12Rik; Mib; mindbomb"
ORIGIN      
        1 cttcccatcc cgttgtcgct gaggagcacc tcgcagttcc tcacgaggcg ccgctcgggc
       61 ctggacgcgg ctgtacccgg atgtggagcc gctctcgccc ggcggggact cggtggcggg
      121 gcggagcgcg gcggcgtgcg cgggggctga gggaagcggg ccccggggcc ccggctccgg
      181 ccgctcgccg gccgctagcg ccgctgccca tcgcggagcc ggctcccggg cgtccaggcc
      241 cgagtgagcg gccgggggcc gctgagggcc ggcggctcgc ctggcctccc gctgcgctcg
      301 cccctgcacc ggggcgagcc tcccccggcc ctccgcgctc ctggccgggg gagagcgagg
      361 tttgctggcg gccgtcgctg tagcccggcc ggggagtccg gggcaagcgg cagagccccc
      421 gcccacggcc gctcgccctc ggcctgcaat ccgccgcctg cgagcctccg cgggcgggcc
      481 tccagtcgga gcgggggccg cgacggccac ggcccggccc gggcgaaagg ctgctcgtgg
      541 accgcgccgc cgccgccccg gtaagtcggc cccacggccc gcggggctcg ctgccgcccc
      601 cgccggcgtc cagagtgcgg cccgggccca gttctctcac gattcccccg ccggcggcgg
      661 cgcccaccgt ccgggcccca atgagtaact cccgcaacaa ccgagtgatg gtggaggggg
      721 tcggcgcccg cgtagtgcgc ggcccggact ggaagtgggg caagcaggac ggcggcgagg
      781 gccatgtagg cactgtacgg agcttcgaga gtccggagga ggtcgtggtg gtgtgggaca
      841 acggcacggc cgccaactac cgctgctcag gggcctacga cctgcgtatc ctggacagcg
      901 cgcccaccgg catcaagcat gatggaacta tgtgtgatac ctgccgccag caaccaatca
      961 ttggcattcg atggaaatgt gcagagtgca ccaattatga tttgtgcact gtttgttatc
     1021 atggagataa acatcactta aggcatcgct tttataggat tactacacca ggaagtgaac
     1081 gggttctgtt agagtctcgt agaaaatcta agaagattac agccagagga atctttgctg
     1141 gtgccagagt ggtgcgagga gtggactggc agtgggaaga tcaagatgga ggcaatgggc
     1201 gtagaggaaa ggtcacggag atccaggact ggagtgcatc cagcccacac agtgcagcct
     1261 atgttctctg ggataacggt gctaagaacc tttacagggt tggctttgag ggcatgtctg
     1321 atctcaagtg tgtccaggat gccaaaggag gttctttcta cagagatcat tgccctgtgc
     1381 taggtgagca gaatggcaac aggaatcctg gtggattgca gattggtgac ctggtaaata
     1441 tagaccttga tctagaaatt gtccagtctt tacagcacgg tcatggagga tggaccgatg
     1501 gcatgtttga gactttaact acaactggaa ccgtctgtgg cattgatgaa gatcatgaca
     1561 ttgtagtaca gtatccaagt ggcaatagat ggactttcaa tcctgctgtt ctcactaagg
     1621 caaacattgt ccgaagtggg gatgctgcac agggtgcaga aggaggcacc tcacagtttc
     1681 aagtgggtga tcttgtgcaa gtttgttatg atctggaaag aattaaactt ctccaaagag
     1741 gacatggtga atgggctgaa gcaatgcttc caactttagg taaagttggc cgagtacaac
     1801 agatttattc ggacagtgac ttaaaggtgg aagtctgtgg aacatcttgg acgtataacc
     1861 ctgcagcagt ttccaaggtg gcccctgcag gatcagccat tagcaatgca tctggtgaaa
     1921 gactctccca gcttctgaag aaattatttg aaacccaaga atctggtgac ctcaatgaag
     1981 agctggttaa ggctgctgcc aatggagacg tggctaaagt ggaagatttg ctgaaaagac
     2041 cagatgtgga tgtaaacgga cagtgtgctg gccacacagc aatgcaagct gctagtcaga
     2101 acggacatgt tgatattttg aaattacttt tgaagcaaaa tgttgatgtt gaagcagagg
     2161 ataaagatgg agatcgagcc gttcaccatg cagcctttgg agatgagggt gctgtcatag
     2221 aagtcctaca ccgaggcagt gctgacctga atgctcgtaa taagcgtaga cagacgcctc
     2281 ttcatattgc tgtcaataaa ggtcatcttc aagttgtgaa gactttgttg gattttggtt
     2341 gtcatcccag tctccaggat tctgaaggtg acacccctct ccatgatgcc ataagtaaga
     2401 aacgggatga cattctggca gttcttctgg aagctggagc agatgtgacc atcacgaaca
     2461 ataacgggtt caatgccctg caccatgctg cactgagagg gaaccccagt gcaatgcgtg
     2521 ttttactctc aaaattacca agaccatgga ttgtggatga gaagaaagat gatggttata
     2581 ctgccttgca cctggctgcc ctgaataatc atgtggaagt ggctgagctg ttggtgcatc
     2641 agggaaatgc aaacctggat atccagaatg tgaaccagca aacagcccta caccttgctg
     2701 ttgaacggca acacacccag attgttaggc ttttggtccg tgcaggtgct aagctagaca
     2761 ttcaggataa ggatggagat actcctctgc atgaagcttt gagacatcat actttgtctc
     2821 aactacgtca gctacaagac atgcaagatg tgggcaaggt agacgctgcc tgggaacctt
     2881 caaaaaacac attaataatg ggacttggta cccagggggc tgagaagaag agtgcagcat
     2941 ccattgcgtg tttcttggca gccaatggtg ctgatctcag cattcgaaat aagaagggtc
     3001 agtcaccact ggatctctgt cctgacccaa gtctctgcaa agctttggca aaatgtcata
     3061 aggaaaaagt cagtggccaa gtgggttctc ggagtccttc tatgattagc aacgattctg
     3121 aaactttaga ggaatgtatg gtgtgctcag acatgaagag agatactctt tttggtccgt
     3181 gtggacacat tgcaacctgt tctttgtgtt cccctcgtgt caagaaatgc ctcatttgta
     3241 aagagcaggt tcagtccagg acaaagattg aagaatgtgt ggtgtgctcc gacaagaaag
     3301 cagctgttct ctttcaacct tgtgggcaca tgtgtgcttg tgagaactgt gccagcctga
     3361 tgaaaaagtg tgtgcagtgt cgggcagtgg ttgagcggag ggtgcctttc atcacgtgct
     3421 gtggtggcaa gagctcagaa gacccctctg atgaaatctc aagtgggaat atcccagtgc
     3481 tgcagaagga caaagacaac accaatgtta acgcagatgt gcagaaactg cagcagcagc
     3541 ttcaggacat taaggagcag accatgtgcc ctgtgtgctt ggatcgcctg aagaacatga
     3601 ttttcctctg tggccatgga acatgccagc tctgtggaga ccggatgagt gaatgcccaa
     3661 tttgtcgcaa ggcaattgaa cgaaggattc ttttgtactg attaagaaac acagagtgtt
     3721 ttgttagcta aagtatgtga ttgtgagatc ttagtatgtt ctaaaactag ttgggagtta
     3781 atctaatgag ttaatttgta atttcataat ttcttttttt attttatttt atttttttta
     3841 gactgtaatt agactatata tggaccacca tcacaacgaa aacatacagt gtttgaactt
     3901 gcccgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgttttcct
     3961 cttgaatttg gaacatcaaa tccatgaaaa ctcatagaaa atttgctttt ggctttgaag
     4021 gggaaaatcc tttaaggact gcctttcaca tttttctttt ttaatatgtg catatatgtt
     4081 aaaccttaga cattagctta caaggtcagt attccacatg attatcatga tttcacatgg
     4141 tgaaatgaca tgctgttcta aaaggtcata ttgaaggtga gtggggcaag tcagactatg
     4201 tcatttatgc tcagtgacag aagtgtagta gagatatgcc tcctggctaa ggcagtactg
     4261 aaggaaacat tgtatttcaa gataatttaa agttatccaa atgattgttt ccgcattttt
     4321 aagtaagtga aaatatgtgg taagagaata atgacttaac cttcttttct agacttaaaa
     4381 ggaaaattga ctaacttaaa taaatgaatt atttcaaaat caccagtaga catgaaaggg
     4441 caaattgtag taagaactga aggctagctc ggaatgacac aatcacacct ctacctggtt
     4501 ttcactgttg gacttcttgt attaacttct cactgtttgc atcagctctt tgtagaaaaa
     4561 gatctgtgaa aagcaatttc cgttttccct gtgactgctg acctgggctt gatctaatca
     4621 gtgcgtgcac accgtccttt ctgtgtctct gaggtctcta agctgggtgg tgcattgtaa
     4681 gaggcagcaa tcttttctgt atgtctgtta tttctgacag tgttacggat tctaccttca
     4741 tccagagcct tacttctttt ctctctgata gtaattttac tataaagaac ttagtttttc
     4801 tttataccta cagatgaaca ttctttaaac tgccaacata tactaagata caataaatag
     4861 cttaatgtca gatacttaga aaaggatgct ggggcatttg tggaaaagca tggaaaacac
     4921 atgttgactt ggttaggggg ctaagcattt tgtctcctta aatgagtaat ctttgtatct
     4981 agcctaaatg tatgcacatt gtttcaagat tcttcactgc tagattgtta aaaacaaaaa
     5041 caaaaaccca cctatcagtt atttacaata gtgtccactg tgcgctgtgt ataagcatgc
     5101 actcttaagg atagtgttct ttgaggatag tttcccctgc atttgaatct ggctagcttg
     5161 tgtctgtttt tttgctatag caacttaaag tgggagagca gtgtccagcc tgctgtgctg
     5221 tgctgtgctg tgctgtgctg tgctgtgctg ctgctggtta cttgcggccc agtgagctga
     5281 gctgctcgtt gtcagcaggc tcagcagtcc catgtctgtg ttgagctgag taactgcttt
     5341 gtttctcttt cgtgtttttg agatagagtc tcactatgta ttctaagctg acatggaacc
     5401 tactgtgtag ctcaggctag cctcaacctt atgatcttcc tgcctctgcc ttccaattgt
     5461 ggggttgtag gatggcgatc ccacatattt aacaacttta aattattttg gagggtacaa
     5521 tccttattag tttcaaatta ggaatgttct attgtaagga agcaatgagg ctattttttt
     5581 ctaagtataa tttgaagagt ttttttgttt ttttaaagat acatctgatg atacgatgtt
     5641 tacataaaga gacaaactat cttcttgcag acttggaaag agtagaaatg aggatctttt
     5701 cttccttgcc ataggctgcc cacctctttc tacttgtcat caggcaagga ggggtggctg
     5761 ctatacagtc tgccagcccg agaacctgtg tgccacagct gtatcccact tcactccttt
     5821 ctctgtctcc caggctgcag tgccaagtct tatcctgtaa agattctatc tgttaaggga
     5881 tagacctcct gacagcttca tcatctccac gcagagcaga gcacactagc ttaggagaga
     5941 gaagccgtca agtctctgta gtgctgggga cttacagctt tctctttgtc actgggccat
     6001 ttatgttatg tatagtatgg acagctgcac ttctcttcag gtcttcaggc atctaaaatt
     6061 attgtaaatt tgttttgggc ttacaggaga tggtaagacc aggaactgaa tcatgccagt
     6121 gacttcctcc tgcactctga aactacctct tagtctcttt acaacacttg gccctttggg
     6181 gatctttcct tctagttacc caaacctctc tttcctgtgc ctcagaagaa ttcttacttt
     6241 tccttagaat tattattaca atgacatgca ttcgtttgtt tttttttaaa gtattggact
     6301 tgggctaagt ggggggcagg aaagaaggtc agtgtcttgt gtctgtggac aacaggatag
     6361 ggtcctgagg catgttagca ttcctcactg gtgtttcagg agagctgtcg ggagcaatat
     6421 tagattccct ctctatgccc ctcggcccac cacattttct cctttattca cgaagaccgt
     6481 cggctttctc actgagcaca gacataactt atcaggcgct gctgccacag ctgctcttgt
     6541 tcacatagtt taacatcctt acctgtgctg tacacatgca gaattttatt gggagtgggg
     6601 ttttcaagag aatataattt tatattagtg taagaatatt atactaggca aatgtttagt
     6661 tatctgaatt acgcttaagg tattgtaatt taataatggc aggttaacca gaagtctact
     6721 gaaacgtaaa attaactgga aataattcct taaattttat atttttgaag actttagcct
     6781 gaaacaacct tttactttag aatgttgaaa atgcatgctg cttaagattt gtctagttca
     6841 aaattcaaac cttgctgggt catttttaca cagggaacat cctggtagcc atgtattgcc
     6901 tggtggatgt tttttgttta ttttgaattc tggacacttt tgggataaag aatagttaaa
     6961 cttgccacct aggacaaagc tggatgtagc agagctggtt tgggggtttg gggttgcaac
     7021 aaatagaaaa caaaaacagt gagctcagag gaatggagca tcatgtagga tttgccacgg
     7081 tattggtggg gagggcgtat ttaactaaaa catagaaggg ggtctataag gtcacttctt
     7141 catatggctt gtcagttgtt ttgtaaatta cagactttaa cacgacttgc tcttttaagc
     7201 ccaaaaggaa aaacatttat cctttcagag tgtctggaat gtttatagta ataatttcta
     7261 taaaaacact catggataca tttttggtgt tttttttttt ttaaattgca gtgagaacca
     7321 catatcataa ggtttgtcgt cctgactgct tctaaacatg cagttcagaa gggtcacttc
     7381 ctctccgttt gttgggaaac acgcatagaa ctttatcctg cacctatcaa aggcttcctt
     7441 cttccttcca tctgggcaac agggttctga ctacttagaa caccagcact cagattgctg
     7501 actagagtca tttgtgtttg caagacccaa acctgggtca acatcaggct cctgagattt
     7561 gaggaagggc acctctcccc tgccataact gctatttttt ttacatgtat gttaattgaa
     7621 agagtttatt atttcgttat gagccagagg ggggaagcac attttaaata tgggaaataa
     7681 gttagctttg gttttgtact gtattgggaa ggtaatagat ttttcacaag tgatttgtgc
     7741 cttgttataa atgtcaattg tcattgagtc tacttgtcaa agtcacagaa tacatttttc
     7801 tttcaaaatg gcaacctgat ttagcttgtt tgaatattac aataggaatg cttactgata
     7861 ctacttgata atcatgtata tccttttgac ttacacacac aaacacacat gagacaacag
     7921 gattgataat tgcagttgta tgtctttaaa cactaaacgt aggaagcttt ttaatgttgc
     7981 cttgtgtgtg tgtgttgctt tactgcagtg agggagaaat cctttgcacc aagcttcagt
     8041 ggctgataag tggctagcag tgggtaaaat tacaatgtaa aaataggggt ggatagttaa
     8101 taacgttaag ttgctacctt ggcattttct acctcctttt ctacgagaat actgcttttc
     8161 tttcacccat ctagcatctt gtcttatttg tgggagaaaa ggaaacaacc tgaagataaa
     8221 gttagatttt cttttttttt ttttttgaga atttaagcaa tagatacagg aaaagtggcc
     8281 tgtcacatta atttggtgcc taaatgtaaa ggtttgaact gataaataaa actgtctaag
     8341 aaggagagta gtgctgagta tttggactgt cctgtatagt tctcttgatt ttatttcata
     8401 aacatgactt ctttcttcaa aagcaaagtg gttttctaat tttttatgaa ataagcaaat
     8461 atgccatttt gtctgagtac agtttcagga agctattgaa agttctggct cagggctttt
     8521 taacagttaa agcaattgtt aatatatttt ggaaactcca tctgcataac tcttcagtgc
     8581 cttgattaac ttggcaacac ttaaaaaaac tttgtactag gtaaccttca gtgcaccagt
     8641 catgatatgc acgttctgat catactgcta agcatgtgca agaacacctc tacgtgtggg
     8701 tttgcataat tctataaggg aagtgttcta catttgcact tgatactttt tttttattat
     8761 tattactctg tttttttaca aactgattat tcaaggccat ggcactgtta ccagaaaaat
     8821 ggtgtttgct gtctatatgc aagtctattt agctgctggg gagaggacag agctcacact
     8881 ttgtatctaa tacaaaaaga agatcaatac aaaaaaaaaa gatcatcttc ctctcaagtg
     8941 gaaaccaaac caagccaaac cctttgttta agtggctcag ttaaatgatt aattgaaagg
     9001 aaatcatgaa ttgcatgtca ttcttcatgc tgatctgtag actaaaagca tgtttttctg
     9061 tctccttcag gatgtgtctg ccattctaag tacagtatat tcaaatggta gaaaaaaaag
     9121 ctgactcaaa ggttttacgc tttgtaattt ttccagttct ttttagcttt caatgttgga
     9181 ataaatttaa gtcttgttta tggctaaagt ttctgtatgg aaaaatgatt cttgcttatt
     9241 agcttttgtt taagaaaaaa agcttagtaa aaactaagct tttaaaaata aatgcaaaca
     9301 tttaccatta ataaaatctg tggtgtaata tgtttttctc tgactttttg gaggctattc
     9361 tttataataa cataaatgaa tttttatttt taaggcattt gaaaacaaat ctcaatacat
     9421 ccaaaaaatc ctattattgg aaagaaaatt accatatctt tgagaaacaa ggacatctct
     9481 ccaactcaat gtaagaagta agcgtccaca tctagaaggt tcttgttgtg agtgctcaca
     9541 tgtcaggcag gagcctgtct aagggtcaga ggagcttgaa tgctttagtg cctaatcttt
     9601 caggatacga tgtgtttgga cagtaacctg aatagaacaa acttgatcaa agaagccaga
     9661 caggaaggaa ggactcagtc tttctctctc tatatgtaga taacttatat ttttaaaaag
     9721 cttattcctc tctgggagac agatatatgt gtgtgtgtat atatatgtat gtctgtgtat
     9781 aaaattttta gaaaggagat attttacagc aatgatgtat ctctcctgtt cagtgttttg
     9841 tgactaatca gtcattgaat agtttggggg tattttattt ttaaagttgc tagacttact
     9901 ttgtaaagtc attgaaaggt ttttctaatt ctagtttaaa agatgcatat ctcaaatgtc
     9961 taagagaaaa tccattgaaa acaaaaatct ttaattgata aaagcacatg tgttccaact
    10021 gaatttattt tggataacct ctaattaatt gtatttgtgt ttatatctat tactgtttaa
    10081 gtagatatct catggtcata ttttttatta aattgaaact gtaggtttaa taataaaaga
    10141 cattttagaa aata
//
