LOCUS       NM_146034               2928 bp    mRNA    linear   ROD 08-AUG-2024
DEFINITION  Mus musculus MIA SH3 domain ER export factor 2 (Mia2), transcript
            variant 3, mRNA.
ACCESSION   NM_146034
VERSION     NM_146034.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,T., Wang,Y., Yu,L., Liu,J., Wang,T., Sun,P., Feng,Y., Zhang,D.,
            Shi,L., He,K., Zhao,L. and Xu,Z.
  TITLE     Mea6/cTAGE5 cooperates with TRAPPC12 to regulate PTN secretion and
            white matter development
  JOURNAL   iScience 27 (3), 109180 (2024)
   PUBMED   38439956
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2928)
  AUTHORS   Wang,X.T., Zhou,L., Cai,X.Y., Xu,F.X., Xu,Z.H., Li,X.Y. and Shen,Y.
  TITLE     Deletion of Mea6 in Cerebellar Granule Cells Impairs Synaptic
            Development and Motor Performance
  JOURNAL   Front Cell Dev Biol 8, 627146 (2021)
   PUBMED   33718348
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2928)
  AUTHORS   Jin,L., Chen,Y., Crossman,D.K., Datta,A., Vu,T., Mobley,J.A.,
            Basu,M.K., Scarduzio,M., Wang,H., Chang,C. and Datta,P.K.
  TITLE     STRAP regulates alternative splicing fidelity during lineage
            commitment of mouse embryonic stem cells
  JOURNAL   Nat Commun 11 (1), 5941 (2020)
   PUBMED   33230114
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2928)
  AUTHORS   Wang,X.T., Cai,X.Y., Xu,F.X., Zhou,L., Zheng,R., Ma,K.Y., Xu,Z.H.
            and Shen,Y.
  TITLE     MEA6 Deficiency Impairs Cerebellar Development and Motor
            Performance by Tethering Protein Trafficking
  JOURNAL   Front Cell Neurosci 13, 250 (2019)
   PUBMED   31244610
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2928)
  AUTHORS   Pitman,J.L., Bonnet,D.J., Curtiss,L.K. and Gekakis,N.
  TITLE     Reduced cholesterol and triglycerides in mice with a mutation in
            Mia2, a liver protein that localizes to ER exit sites
  JOURNAL   J Lipid Res 52 (10), 1775-1786 (2011)
   PUBMED   21807889
  REMARK    GeneRIF: Mia2 is a novel, hepatic, endoplasmic reticulum-to-Golgi
            trafficking protein that regulates cholesterol metabolism.
REFERENCE   7  (bases 1 to 2928)
  AUTHORS   Lin,S., Ikegami,M., Xu,Y., Bosserhoff,A.K., Malkinson,A.M. and
            Shannon,J.M.
  TITLE     Misexpression of MIA disrupts lung morphogenesis and causes
            neonatal death
  JOURNAL   Dev Biol 316 (2), 441-455 (2008)
   PUBMED   18342301
REFERENCE   8  (bases 1 to 2928)
  AUTHORS   Bosserhoff,A.K., Moser,M. and Buettner,R.
  TITLE     Characterization and expression pattern of the novel MIA homolog
            TANGO
  JOURNAL   Gene Expr Patterns 4 (4), 473-479 (2004)
   PUBMED   15183315
REFERENCE   9  (bases 1 to 2928)
  AUTHORS   Bosserhoff,A.K., Moser,M., Scholmerich,J., Buettner,R. and
            Hellerbrand,C.
  TITLE     Specific expression and regulation of the new melanoma inhibitory
            activity-related gene MIA2 in hepatocytes
  JOURNAL   J Biol Chem 278 (17), 15225-15231 (2003)
   PUBMED   12586826
REFERENCE   10 (bases 1 to 2928)
  AUTHORS   Moser,M., Bosserhoff,A.K., Hunziker,E.B., Sandell,L., Fassler,R.
            and Buettner,R.
  TITLE     Ultrastructural cartilage abnormalities in MIA/CD-RAP-deficient
            mice
  JOURNAL   Mol Cell Biol 22 (5), 1438-1445 (2002)
   PUBMED   11839810
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132607.4.
            
            On Mar 4, 2019 this sequence version replaced NM_146034.4.
            
            Summary: This gene encodes a protein that is involved in
            endoplasmic reticulum-to-Golgi trafficking and regulation of
            cholesterol metabolism. Three major classes of transcripts are
            generated from this gene- melanoma inhibitory activity 2-specific
            transcripts, cTAGE family member 5-specific transcripts and
            transcripts that include exons from both these transcript species.
            Additionally, alternative splicing in these transcripts results in
            multiple transcript variants encoding diverse isoforms. A mutation
            in this gene (couch-potato or cpto) may result in low levels of
            plasma cholesterol and triglycerides. [provided by RefSeq, Sep
            2016].
            
            Transcript Variant: This variant (3) lacks several 5' exons and
            reflects the use of an alternate internal promoter, compared to
            variant 1. It encodes the cTAGE family member 5 protein (isoform
            3), which is shorter and has a distinct N-terminus, compared to
            isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is supported by available conservation data.
            The use of an alternative upstream start codon would result in a
            protein that is 17 aa longer.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026864.1, SRR7652917.455187.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               AC132607.4         126993-127141
            150-281             AC132607.4         133309-133440
            282-303             AC132607.4         134826-134847
            304-392             AC132607.4         134943-135031
            393-464             AC132607.4         141944-142015
            465-491             AC132607.4         143581-143607
            492-602             AC132607.4         144092-144202
            603-674             AC132607.4         145097-145168
            675-779             AC132607.4         146709-146813
            780-902             AC132607.4         151601-151723
            903-1017            AC132607.4         155498-155612
            1018-1064           AC132607.4         156836-156882
            1065-1155           AC132607.4         157421-157511
            1156-1294           AC132607.4         160126-160264
            1295-1396           AC132607.4         166179-166280
            1397-1457           AC132607.4         167503-167563
            1458-1493           AC132607.4         168202-168237
            1494-1561           AC132607.4         169770-169837
            1562-1644           AC132607.4         171351-171433
            1645-1773           AC132607.4         173576-173704
            1774-1932           AC132607.4         177095-177253
            1933-2049           AC132607.4         181432-181548
            2050-2108           AC132607.4         182974-183032
            2109-2343           AC132607.4         185556-185790
            2344-2928           AC132607.4         186881-187465
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 26.0 cM"
     gene            1..2928
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /note="MIA SH3 domain ER export factor 2"
                     /db_xref="GeneID:338320"
                     /db_xref="MGI:MGI:2159614"
     exon            1..149
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..2507
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /note="isoform 3 is encoded by transcript variant 3;
                     melanoma inhibitory activity protein 2; melanoma
                     inhibitory activity 2; meningioma expressed antigen 6
                     (coiled-coil proline-rich); CTAGE family, member 5;
                     cutaneous T-cell lymphoma-associated antigen 5 homolog;
                     protein cTAGE-5; CTAGE family member 5 ER export factor"
                     /codon_start=1
                     /product="melanoma inhibitory activity protein 2 isoform
                     3"
                     /protein_id="NP_666146.3"
                     /db_xref="CCDS:CCDS36458.2"
                     /db_xref="GeneID:338320"
                     /db_xref="MGI:MGI:2159614"
                     /translation="MELEGLGEEVVAALPEDMRADFNPSGFSLELAVCVLSVGLLAVV
                     LFLWRGFRSIRSRFYVGREKKLALELSALIEEKCKLLDKVSIVQKEYEGLESSLKEAS
                     FEKESTEAQSLEFVEGSQISEATYENLEQSKSKLEDEILLLEEKLEEERAKHSEQDEL
                     MADISKRIQSLEDESKSLKSQVAEAKTTFRIFEINEERLKGAIKDALNENSQLQESQK
                     QLLQETEMMKEQVNDLDKQKVALEESRAQAEQALSEKESQIETLVTSLLKMKDWAAVL
                     GEADDGNLDLDMKSGLENTAALDNQPKGALKKLIYAAKLNASLKALEGERNQVYTQLS
                     EVDQVKEDLTEHIKSLESKQASLQSEKTEFESESQKLQQKLKVITELYQENEMKLHRK
                     LTVEENYRLEKEEKLSKVDEKISHATEELETCRQRAKDLEEELERTIHSYQGQVISHE
                     KKAHDNWLAARTLERNLNDLRKENAHNRQKLTETEFKFELLEKDPYALDVPNTAFGRE
                     HSPYGPSPLGRPPSETRAFLSPPTLLEGPLRLSPLLPGGGGRGSRGPENLLDHQMNTE
                     RGESSYDRLSDAPRAPSDRSLSPPWEQDRRMTAHPPPGQPYSDPALQRQDRFYPNSGR
                     LSGPAELRSYNMPSLDKVDGPVPSEMESSGNGTKDNLGNSNVPDSPIPAECEAAGRGF
                     FPPPFPPVRDPLFPVDPRSQFMRRGPSFPPPPPGSIYAAPRDYFPPRDFPGPPLPPFP
                     GRTVYAPRGFPPYLPPRAGFFPPPPHPESRSELPPDLIPPSKEPAADPPETQEA"
     exon            150..281
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            282..303
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            304..392
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            393..464
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            465..491
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            492..602
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            603..674
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            675..779
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            780..902
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1017
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1064
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1155
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1294
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1396
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1457
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1493
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1561
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1644
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1773
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1932
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..2049
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2108
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2343
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2928
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2638..2643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /note="hexamer: AATAAA"
     polyA_site      2660
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
     regulatory      2862..2867
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /note="hexamer: AATAAA"
     polyA_site      2884
                     /gene="Mia2"
                     /gene_synonym="Ctage5; D12Bwg0579e; Mea6; Mgea; Mgea6"
                     /note="major polyA site"
ORIGIN      
        1 acactctgtg tggaaacggg agtgcagaag gtattaaaat ctggactctt tctcaaggat
       61 ctgaagtagt caggagagct ttagttacat gtccgggtgt tttaacatga atgtcctagg
      121 acatggaatt ggaaggtctg ggtgaggagg ttgtggcagc attgcctgaa gatatgagag
      181 cagactttaa tcccagtggt ttttcgttgg aattggcagt atgtgtgctt tccgttggac
      241 tgcttgccgt ggtcttgttt ctgtggcgag gcttccgatc aattcgaagc cgattttatg
      301 tggggagaga aaagaaactt gctcttgagc tttctgcact aattgaagaa aaatgtaaac
      361 tgcttgacaa agtcagcatt gttcaaaagg agtatgaagg cttagagtca tctttaaagg
      421 aggccagctt tgagaaggag tcaacagaag cacaaagttt ggagtttgtt gaaggatcac
      481 aaatatcaga ggccacctat gaaaatctgg aacaatccaa atctaaactt gaagatgaaa
      541 tactactcct agaggaaaag ctggaagaag agcgagctaa acattctgaa caagacgagc
      601 tgatggctga tatttcaaaa aggatacagt ccctagaaga tgaatcaaaa tctctcaaat
      661 cgcaagtagc tgaagccaaa acaaccttta gaatatttga aatcaatgaa gagcgactca
      721 agggagcaat aaaagatgcc ttgaatgaaa attctcagct tcaggaaagc cagaaacagc
      781 ttttacagga aactgaaatg atgaaagaac aagtgaatga tctcgataaa cagaaagtgg
      841 cattggaaga gtccagagca caggcggaac aagctctgag tgagaaggag agtcagattg
      901 agactctggt cacgagcctg ctaaagatga aggattgggc tgctgtgctt ggagaagcag
      961 atgatgggaa tctggacttg gacatgaaga gtggcctaga aaacactgct gccttagata
     1021 atcagccaaa gggcgctttg aagaagctga tttatgcagc taagttaaat gcttctttaa
     1081 aagccttgga aggagaacga aatcaagttt acactcagtt atctgaagtg gatcaagtaa
     1141 aagaagacct tacagagcat atcaaaagtc ttgagtctaa acaagcatct ttgcagtcag
     1201 aaaagacaga gtttgaaagt gagagccaga aacttcagca gaaactgaaa gtgataaccg
     1261 agctgtacca agaaaatgaa atgaaacttc acaggaaatt aacagtagaa gaaaattacc
     1321 gattagagaa agaagaaaaa ctttccaaag tagatgagaa aatcagccat gcgaccgagg
     1381 agctggagac ctgcaggcag cgtgccaagg atcttgaaga agagctggag agaacaattc
     1441 attcttacca ggggcaggtt atttctcatg aaaaaaaagc acatgataat tggctggcag
     1501 ctcggactct tgaaagaaat ctcaatgatt taagaaaaga aaatgcccac aacagacaaa
     1561 aattaactga aacagagttt aaatttgaac ttttagaaaa agatccttat gcacttgatg
     1621 ttccaaacac agcatttggc agagagcact ccccatatgg tccctcacca ttgggccgac
     1681 ctccatctga aacgagagct tttctctctc ctccaacttt gttggagggt ccactaagac
     1741 tctcaccttt gcttccaggg ggaggaggaa gaggctcacg aggccctgag aaccttctgg
     1801 accatcagat gaacactgaa agaggagagt caagctatga cagattatct gatgctccca
     1861 gagcaccttc cgacaggtcc ctgtcacctc cgtgggaaca agatcgaagg atgacagccc
     1921 atccgccacc aggccaacca tattctgatc cagctctaca aaggcaagat cggttttatc
     1981 ctaattctgg tagactctct ggaccagcag aactcagaag ttataatatg ccctctttgg
     2041 ataaagtgga tgggccagta ccctcagaaa tggagtccag tggaaacggt accaaagata
     2101 atcttggtaa ctctaatgtg cctgattctc ctatccctgc tgaatgcgaa gcagctggcc
     2161 gtggcttctt ccctccaccc tttcctccag tcagagaccc attgtttcca gtggatccaa
     2221 ggagccagtt catgagaagg ggcccctctt tccccccacc tcctccagga agcatctatg
     2281 cagctcctcg agattatttt ccaccaaggg acttccctgg cccaccactt cctccgttcc
     2341 cagggagaac tgtgtacgca ccgaggggct ttccacctta ccttcccccc agagctgggt
     2401 tcttcccccc acccccacat cctgaaagta gaagtgagct ccccccagac ttgattccgc
     2461 cttcaaaaga gcctgcagct gaccctccag aaacacagga ggcctgacag cgttacgtct
     2521 taccggcagt catttccgtc tcattttcag tttaagtaac tgctgttact tatgtgatta
     2581 tatttttgct caaattgaag cttaatggaa ttataattct caggatagta ttttgtaaat
     2641 aaagatgatt taaatatgaa tcttatgggt aaattatttc cattttattt tattctagat
     2701 agtataacta ttttactttg attacctcat ctataattat ggtagtttta tatatgtaat
     2761 cttatagttg gggatatctc aaactcaaag gctgtgtctt tataccaggg atgtgtttac
     2821 catggttgta ggcaatgtaa aagtaacttc atgtttaatc aaataaaatt ttaattgatt
     2881 taaagtcttg tttggtatta ataataataa aactagccag tttcctgt
//
