LOCUS       NM_008597                632 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus matrix Gla protein (Mgp), mRNA.
ACCESSION   NM_008597
VERSION     NM_008597.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Zhang,L., Cai,X., Ma,F., Qiao,X., Ji,J., Ma,J.A., Vergnes,L.,
            Zhao,Y., Yao,Y., Wu,X. and Bostrom,K.I.
  TITLE     Two-step regulation by matrix Gla protein in brown adipose cell
            differentiation
  JOURNAL   Mol Metab 80, 101870 (2024)
   PUBMED   38184275
REFERENCE   2  (bases 1 to 632)
  AUTHORS   Queen,R., Crosier,M., Eley,L., Kerwin,J., Turner,J.E., Yu,J.,
            Alqahtani,A., Dhanaseelan,T., Overman,L., Soetjoadi,H., Baldock,R.,
            Coxhead,J., Boczonadi,V., Laude,A., Cockell,S.J., Kane,M.A.,
            Lisgo,S. and Henderson,D.J.
  TITLE     Spatial transcriptomics reveals novel genes during the remodelling
            of the embryonic human arterial valves
  JOURNAL   PLoS Genet 19 (11), e1010777 (2023)
   PUBMED   38011284
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 632)
  AUTHORS   Gourgas,O., Lemire,G., Eaton,A.J., Alshahrani,S., Duker,A.L.,
            Li,J., Carroll,R.S., Mackenzie,S., Nikkel,S.M., Bober,M.B.,
            Boycott,K.M. and Murshed,M.
  CONSRTM   Care4Rare Canada Consortium
  TITLE     Specific heterozygous variants in MGP lead to endoplasmic reticulum
            stress and cause spondyloepiphyseal dysplasia
  JOURNAL   Nat Commun 14 (1), 7054 (2023)
   PUBMED   37923733
  REMARK    GeneRIF: Specific heterozygous variants in MGP lead to endoplasmic
            reticulum stress and cause spondyloepiphyseal dysplasia.
            Erratum:[Nat Commun. 2024 Apr 30;15(1):3655. doi:
            10.1038/s41467-024-47898-x.. PMID: 38688929]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 632)
  AUTHORS   Nieddu,V., Melocchi,V., Battistini,C., Franciosa,G., Lupia,M.,
            Stellato,C., Bertalot,G., Olsen,J.V., Colombo,N., Bianchi,F. and
            Cavallaro,U.
  TITLE     Matrix Gla Protein drives stemness and tumor initiation in ovarian
            cancer
  JOURNAL   Cell Death Dis 14 (3), 220 (2023)
   PUBMED   36977707
  REMARK    GeneRIF: Matrix Gla Protein drives stemness and tumor initiation in
            ovarian cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 632)
  AUTHORS   Hui,S.T., Gong,L., Swichkow,C., Blencowe,M., Kaminska,D.,
            Diamante,G., Pan,C., Dalsania,M., French,S.W., Magyar,C.E.,
            Pajukanta,P., Pihlajamaki,J., Bostrom,K.I., Yang,X. and Lusis,A.J.
  TITLE     Role of Matrix Gla Protein in Transforming Growth Factor-beta
            Signaling and Nonalcoholic Steatohepatitis in Mice
  JOURNAL   Cell Mol Gastroenterol Hepatol 16 (6), 943-960 (2023)
   PUBMED   37611662
  REMARK    GeneRIF: Role of Matrix Gla Protein in Transforming Growth
            Factor-beta Signaling and Nonalcoholic Steatohepatitis in Mice.
REFERENCE   6  (bases 1 to 632)
  AUTHORS   Luo,G., Ducy,P., McKee,M.D., Pinero,G.J., Loyer,E., Behringer,R.R.
            and Karsenty,G.
  TITLE     Spontaneous calcification of arteries and cartilage in mice lacking
            matrix GLA protein
  JOURNAL   Nature 386 (6620), 78-81 (1997)
   PUBMED   9052783
REFERENCE   7  (bases 1 to 632)
  AUTHORS   Luo,G., D'Souza,R., Hogue,D. and Karsenty,G.
  TITLE     The matrix Gla protein gene is a marker of the chondrogenesis cell
            lineage during mouse development
  JOURNAL   J Bone Miner Res 10 (2), 325-334 (1995)
   PUBMED   7754814
REFERENCE   8  (bases 1 to 632)
  AUTHORS   Tashiro,K., Tada,H., Heilker,R., Shirozu,M., Nakano,T. and Honjo,T.
  TITLE     Signal sequence trap: a cloning strategy for secreted proteins and
            type I membrane proteins
  JOURNAL   Science 261 (5121), 600-603 (1993)
   PUBMED   8342023
REFERENCE   9  (bases 1 to 632)
  AUTHORS   Johnson,T.L., Sakaguchi,A.Y., Lalley,P.A. and Leach,R.J.
  TITLE     Chromosomal assignment in mouse of matrix Gla protein and bone Gla
            protein genes
  JOURNAL   Genomics 11 (3), 770-772 (1991)
   PUBMED   1774075
REFERENCE   10 (bases 1 to 632)
  AUTHORS   Ikeda,T., Yamaguchi,A., Icho,T., Tsuchida,N. and Yoshiki,S.
  TITLE     cDNA and deduced amino acid sequence of mouse matrix gla protein:
            one of five glutamic acid residues potentially modified to gla is
            not conserved in the mouse sequence
  JOURNAL   J Bone Miner Res 6 (9), 1013-1017 (1991)
   PUBMED   1686347
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC122804.4, AK018719.1 and
            BY702829.1.
            
            On Sep 21, 2016 this sequence version replaced NM_008597.3.
            
            Summary: This gene encodes a member of the osteocalcin/matrix Gla
            family of proteins. The encoded vitamin K-dependent protein is
            secreted by chondrocytes and vascular smooth muscle cells, and
            functions as a physiological inhibitor of ectopic tissue
            calcification. This protein also inhibits angiogenesis. Mice
            lacking a functional copy of this gene exhibit impaired
            differentiation of endothelial cells, reduced stature, and
            calcification and rupture of the vasculature leading to premature
            death. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY702829.1, CK626150.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AC122804.4         41460-41477
            19-616              AK018719.1         2-599
            617-632             BY702829.1         600-615
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 66.72 cM"
     gene            1..632
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /note="matrix Gla protein"
                     /db_xref="GeneID:17313"
                     /db_xref="MGI:MGI:96976"
     exon            1..154
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..408
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /note="matrix gamma-carboxyglutamate (gla) protein"
                     /codon_start=1
                     /product="matrix Gla protein precursor"
                     /protein_id="NP_032623.1"
                     /db_xref="CCDS:CCDS20659.1"
                     /db_xref="GeneID:17313"
                     /db_xref="MGI:MGI:96976"
                     /translation="MKSLLPLAILAALAVATLCYESHESMESYEISPFINRRNANTFM
                     SPQQRWRAKAQKRVQERNKPAYEINREACDDYKLCERYAMVYGYNAAYNRYFRQRRGA
                     KY"
     exon            155..187
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /inference="alignment:Splign:2.1.0"
     exon            188..263
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /inference="alignment:Splign:2.1.0"
     exon            264..617
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      592..597
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /note="hexamer: AATAAA"
     polyA_site      617
                     /gene="Mgp"
                     /gene_synonym="Mglap"
                     /note="major polyA site"
ORIGIN      
        1 ccagccttcc ccagcagcat gtaggagcct ctcccctctg ctgctgctgc tgcgccagac
       61 acagaggcag actcacagga cacccgagac accatgaaga gcctgctccc tctggccatc
      121 ctggctgcgc tggccgtggc aaccctgtgc tacgaatctc acgaaagcat ggagtcctat
      181 gaaatcagtc ccttcatcaa caggagaaat gccaacacct ttatgtcccc tcagcagagg
      241 tggcgagcta aagcccaaaa gagagtccag gaacgcaaca agcctgccta cgagatcaac
      301 agagaggcct gcgatgacta caagctgtgt gagcgctacg ccatggtcta cggctacaac
      361 gctgcctaca accgctactt caggcagcgc cgaggagcca aatattagcg cgaagaaaca
      421 gtcatttggt tgtggagttt cgttttatat ctcctgcagt agcattactg aagtatacag
      481 acacgcatgt gttgcttgct ccttacatga tctcctagct ggctggccca ctccttcctt
      541 ctgcgggttg aaagtaatga aagaacagta ttaagaagtg tgtttatata taataaaatt
      601 ctggtttgat acgttcaaaa aaaaaaaaaa aa
//
