LOCUS       NM_001412697            3188 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus O-6-methylguanine-DNA methyltransferase (Mgmt),
            transcript variant 3, mRNA.
ACCESSION   NM_001412697 XM_006507405
VERSION     NM_001412697.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Gonzalez-Aponte,M.F., Damato,A.R., Trebucq,L.L., Simon,T.,
            Cardenas-Garcia,S.P., Cho,K., Patti,G.J., Golombek,D.A.,
            Chiesa,J.J., Rubin,J.B. and Herzog,E.D.
  TITLE     Circadian regulation of MGMT expression and promoter methylation
            underlies daily rhythms in TMZ sensitivity in glioblastoma
  JOURNAL   J Neurooncol 166 (3), 419-430 (2024)
   PUBMED   38277015
  REMARK    GeneRIF: Circadian regulation of MGMT expression and promoter
            methylation underlies daily rhythms in TMZ sensitivity in
            glioblastoma.
REFERENCE   3  (bases 1 to 3188)
  AUTHORS   Benjaskulluecha,S., Boonmee,A., Haque,M., Wongprom,B.,
            Pattarakankul,T., Pongma,C., Sri-Ngern-Ngam,K., Keawvilai,P.,
            Sukdee,T., Saechue,B., Kueanjinda,P. and Palaga,T.
  TITLE     O6-methylguanine DNA methyltransferase regulates
            beta-glucan-induced trained immunity of macrophages via farnesoid X
            receptor and AMPK
  JOURNAL   iScience 27 (1), 108733 (2023)
   PUBMED   38235325
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3188)
  AUTHORS   Sinha,A., Ahmed,S., George,C., Tsagaris,M., Naufer,A., von Both,I.,
            Tkachyova,I., van Eede,M., Henkelman,M. and Schulze,A.
  TITLE     Magnetic resonance imaging reveals specific anatomical changes in
            the brain of Agat- and Gamt-mice attributed to creatine depletion
            and guanidinoacetate alteration
  JOURNAL   J Inherit Metab Dis 43 (4), 827-842 (2020)
   PUBMED   31951021
  REMARK    GeneRIF: Magnetic resonance imaging reveals specific anatomical
            changes in the brain of Agat- and Gamt-mice attributed to creatine
            depletion and guanidinoacetate alteration.
REFERENCE   5  (bases 1 to 3188)
  AUTHORS   Sasani,A., Hornig,S., Grzybowski,R., Cordts,K., Hanff,E.,
            Tsikas,D., Boger,R., Gerloff,C., Isbrandt,D., Neu,A., Schwedhelm,E.
            and Choe,C.U.
  TITLE     Muscle phenotype of AGAT- and GAMT-deficient mice after simvastatin
            exposure
  JOURNAL   Amino Acids 52 (1), 73-85 (2020)
   PUBMED   31853708
  REMARK    GeneRIF: Muscle phenotype of AGAT- and GAMT-deficient mice after
            simvastatin exposure.
REFERENCE   6  (bases 1 to 3188)
  AUTHORS   Kawate,H., Ihara,K., Kohda,K., Sakumi,K. and Sekiguchi,M.
  TITLE     Mouse methyltransferase for repair of O6-methylguanine and
            O4-methylthymine in DNA
  JOURNAL   Carcinogenesis 16 (7), 1595-1602 (1995)
   PUBMED   7614694
REFERENCE   7  (bases 1 to 3188)
  AUTHORS   Roy,R., Shiota,S., Kennel,S.J., Raha,R., von Wronski,M., Brent,T.P.
            and Mitra,S.
  TITLE     A comparative study of the biochemical properties of human and
            mouse recombinant O6-methylguanine-DNA methyltransferases
  JOURNAL   Carcinogenesis 16 (2), 405-411 (1995)
   PUBMED   7532116
REFERENCE   8  (bases 1 to 3188)
  AUTHORS   Rouleau,J., Tanigawa,G. and Szyf,M.
  TITLE     The mouse DNA methyltransferase 5'-region. A unique housekeeping
            gene promoter
  JOURNAL   J Biol Chem 267 (11), 7368-7377 (1992)
   PUBMED   1559980
REFERENCE   9  (bases 1 to 3188)
  AUTHORS   Shiota,S., von Wronski,M.A., Tano,K., Bigner,D.D., Brent,T.P. and
            Mitra,S.
  TITLE     Characterization of cDNA encoding mouse DNA repair protein
            O6-methylguanine-DNA methyltransferase and high-level expression of
            the wild-type and mutant proteins in Escherichia coli
  JOURNAL   Biochemistry 31 (7), 1897-1903 (1992)
   PUBMED   1371399
REFERENCE   10 (bases 1 to 3188)
  AUTHORS   Santibanez-Koref,M., Elder,R.H., Fan,C.Y., Cawkwell,L., McKie,J.H.,
            Douglas,K.T., Margison,G.P. and Rafferty,J.A.
  TITLE     Isolation and partial characterization of murine
            O6-alkylguanine-DNA-alkyltransferase: comparative sequence and
            structural properties
  JOURNAL   Mol Carcinog 5 (2), 161-169 (1992)
   PUBMED   1554415
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132594.3, AC148997.1 and AC135639.4.
            
            On Oct 14, 2022 this sequence version replaced XM_006507405.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363658.4061521.1,
                                           SRR13862101.2363639.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-362               AC132594.3         123583-123944       c
            363-499             AC132594.3         97347-97483         c
            500-660             AC148997.1         100961-101121       c
            661-800             AC135639.4         208221-208360       c
            801-3188            AC135639.4         199577-201964       c
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 82.07 cM"
     gene            1..3188
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="O-6-methylguanine-DNA methyltransferase"
                     /db_xref="GeneID:17314"
                     /db_xref="MGI:MGI:96977"
     exon            1..362
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..1010
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /EC_number="2.1.1.63"
                     /note="isoform 2 is encoded by transcript variant 3;
                     methylated-DNA--protein-cysteine methyltransferase;
                     O-6-methylguanine-DNA-alkyltransferase;
                     6-O-methylguanine-DNA methyltransferase;
                     O-6-alkylguanine-DNA alkyltransferase"
                     /codon_start=1
                     /product="methylated-DNA--protein-cysteine
                     methyltransferase isoform 2"
                     /protein_id="NP_001399626.1"
                     /db_xref="GeneID:17314"
                     /db_xref="MGI:MGI:96977"
                     /translation="MGSRALPCGLGVSTAALPVCSCLQRSTAELGRMAETCKMKYSVL
                     DSPLGKMELSGCERGLHGIRLLSGKTPNTDPTEAPATPEVLGGPEGVPEPLVQCTAWL
                     EAYFREPAATEGLPLPALHHPVFQQDSFTRQVLWKLLKVVKFGETVSYQQLAALAGNP
                     KAARAVGGAMRSNPVPILIPCHRVVRSDGAIGHYSGGGQAVKEWLLAHEGIPTGQPAS
                     KGLGLTGTWLKSSFESTSSEPSGRN"
     exon            363..499
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /inference="alignment:Splign:2.1.0"
     exon            500..660
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /inference="alignment:Splign:2.1.0"
     exon            661..800
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /inference="alignment:Splign:2.1.0"
     exon            801..3188
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1076..1081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="hexamer: ATTAAA"
     polyA_site      1104
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="major polyA site"
     regulatory      1225..1230
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="hexamer: ATTAAA"
     polyA_site      1244
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
     regulatory      1557..1562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="hexamer: AATAAA"
     polyA_site      1580
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
     regulatory      3172..3177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
                     /note="hexamer: AAGAAA"
     polyA_site      3188
                     /gene="Mgmt"
                     /gene_synonym="Agat; AGT"
ORIGIN      
        1 acacaggcac tcagaggaac aacgatgcgg gcacgctctc cctgtcagac gcatatggaa
       61 tttggcccgt ggtgggctta tctcttccca ccagcttctt tctttacaca tctaaaatga
      121 caccggcatg taagttcctg cagaaatagc cctgcgttgg gtcagcagca gaagatgcca
      181 ggccattccc agagcacccc gaggccagag ctctgtgtcc tcgacagtgt ccagtgggct
      241 gaggatgatg gctctcggct gagaagattg atgtggccat gggctctcgt gccctcccct
      301 gtgggctggg agtgagcact gcagccctcc ccgtgtgtag ttgtcttcag aggagcacag
      361 cagaacttgg cagaatggct gagacctgca aaatgaaata ctcagtgttg gacagccctt
      421 tggggaagat ggagctgtct ggctgtgagc gaggcctgca tgggatacgg ttgctcagtg
      481 ggaagacccc aaacactgac cccacagagg ccccagctac tcctgaggtg ctcggtgggc
      541 cagagggagt tccagagcct ctggtgcagt gcacagcctg gctggaagcc tatttccgtg
      601 aacccgcagc cacagagggg cttcccttgc ctgctctcca tcaccctgtg ttccagcaag
      661 attcattcac cagacaggtg ttatggaagc tgctgaaggt tgtgaaattc ggagaaacgg
      721 tttcttacca gcaattagca gccctggcag gcaaccccaa agcggctcgt gcagtaggag
      781 gagcaatgag aagcaatccg gtccccatcc tcatcccctg ccacagggtg gttcgcagtg
      841 acggtgccat cggccattac tccggaggag ggcaggctgt gaaggagtgg cttctggccc
      901 atgagggcat cccgaccgga cagccagcct ccaagggctt gggtctgact gggacctggc
      961 tcaagtcatc cttcgagtcg accagctctg agccgtctgg ccgaaattga gtaaccgttt
     1021 gaatgacaca tagatgtaac gcgtgtcgga agcggatgtg tggtggcacc actatattaa
     1081 aagagctgca agtgtcctgg gggacgtgat gtggctgccc tttcttgttt ctacatttta
     1141 cagcagaatg agttcagacg acccacctcg agtgtgtcat cagttccctc ctctgacatg
     1201 gttattgttc tatttttaat gcccattaaa atgggggtga gcgaggaagg cctgatgagc
     1261 cttagggccc ctttgtatga agggagacac atctgacacc tgcaggcaac agctggctac
     1321 cccagcccgt ccacactaaa cagctgaccc caccccgata gggcccccaa ctcatccttc
     1381 aagccattta ccctgtggag atggcagccc ccattcaggc cctaatctcc cacccttgct
     1441 ggctggaggg gagacaggct agcaagcgag tgctctgttg ggggtctcag cattgctccc
     1501 ccatctgcca ggaaagtgtc ttgtatccac ggtggatgca agatgattaa gcagaaaata
     1561 aaatagtacc atcttccaaa cactggtttg gaaatgctga atagcagcat ttgggtcacc
     1621 ctcaaattcc agctctcctt tctttccttg tctttgaagg ctcctagtgg taggtggagg
     1681 agacatggtg cctcctggga gcctcgagga ccaggaacct gtaaaggtcc cagcagccca
     1741 tgttgatctc atgcattgaa ggttatacta tgactcagca catgctgcca ccagcgctgg
     1801 agactgtctc cagatctgcc ataaggcacc acacgatgct tcacaattac atcatcctcc
     1861 caccttcaga gtcgactgtt cccaaaaagg gagaggagac tcagccaggt gcatggctag
     1921 gtaaaacagc atacgattgg aggaacgagc caccaaccgt gcagcggaga gcttggtttt
     1981 atggtggcct ctctgacagc aggtgccact catggcatca tggaatcatg gcctctacag
     2041 aaccctcata ctgcatgcta ccttgacacc cacataaacc tcaggccttt ctccctggct
     2101 aacagtttat tatgtcctca tcttgcatcc agccgcacta actctgtttt tttttttttt
     2161 tttttttttt tttttgagag gcatcaccaa taccacactt tctgaatttg gttttcacct
     2221 atcatcacag agcaaggtgg gtctcattta tgtgagcccc ataactcata agttttcagg
     2281 acagggctgg gcatgcaccc tggcaccctc ccatctggcc tcgccagggt gttttctgct
     2341 catttcatct ctctctgaca tggccagcaa gacccatgga aagggctggg gtaattgtat
     2401 cagagtaacg ccacactggt atctctccag caccaggaaa tcactgtatg atgttttttt
     2461 cctctgcctg aagagagcag aattctcaaa gagccgtgta tgtgtgtgtt ccaacagagg
     2521 gcactgtggc tgcagtgtgc tggggagacc ccagcctcca ctctgccttc agaggagttg
     2581 gaaggtgggc atcagggtcg aagtcaactg gttctgagca ggtcctccac cccgccgtgt
     2641 gggccgtcag accagtgcct tgtacatagg cctcctgtgc ctgggtggcc tatgtaatgg
     2701 gagctttcct tgtgatctct gcacttccaa atagatccag gctcttaaga ggagcctggg
     2761 gctgtacaga catccacggc cgaaccccca gcatgggaca gccacatgcg gccaggacgc
     2821 caaccccatc aaagccacaa gggcaacctg gcagttccag gtagcctgga atgatgagtg
     2881 gacgtgcttg ggcctgccct tggagcgatg cgaaagccgt gtctgctccc ctctccgagg
     2941 gtttccttgg tgctgtcata acaggttcat agatgccccg aggtagccgt tgacagcagc
     3001 aggactcact gcagtgaccg tgtactgctc caaggataaa ctctgcagag gctgacatga
     3061 aacaccagca cggtcgcctg gagccgctct ggctcggggt tgcagcgcag cttgtgaaaa
     3121 tgagagaaag aggaaaggca taaagaatta caaaaatatt ttccaaataa aaagaaatgt
     3181 tgaaacaa
//
