LOCUS       NM_001355688            2711 bp    mRNA    linear   ROD 18-JUL-2024
DEFINITION  Mus musculus mitochondrial genome maintenance exonuclease 1
            (Mgme1), transcript variant 4, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001355688 XM_006500302
VERSION     NM_001355688.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Gonzalez,C.D., Nissanka,N., Van Booven,D., Griswold,A.J. and
            Moraes,C.T.
  TITLE     Absence of both MGME1 and POLG EXO abolishes mtDNA whereas absence
            of either creates unique mtDNA duplications
  JOURNAL   J Biol Chem 300 (4), 107128 (2024)
   PUBMED   38432635
  REMARK    GeneRIF: Absence of both MGME1 and POLG EXO abolishes mtDNA whereas
            absence of either creates unique mtDNA duplications.
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2711)
  AUTHORS   Milenkovic,D., Sanz-Moreno,A., Calzada-Wack,J., Rathkolb,B.,
            Veronica Amarie,O., Gerlini,R., Aguilar-Pimentel,A., Misic,J.,
            Simard,M.L., Wolf,E., Fuchs,H., Gailus-Durner,V., de Angelis,M.H.
            and Larsson,N.G.
  TITLE     Mice lacking the mitochondrial exonuclease MGME1 develop
            inflammatory kidney disease with glomerular dysfunction
  JOURNAL   PLoS Genet 18 (5), e1010190 (2022)
   PUBMED   35533204
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2711)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2711)
  AUTHORS   Basu,S., Xie,X., Uhler,J.P., Hedberg-Oldfors,C., Milenkovic,D.,
            Baris,O.R., Kimoloi,S., Matic,S., Stewart,J.B., Larsson,N.G.,
            Wiesner,R.J., Oldfors,A., Gustafsson,C.M., Falkenberg,M. and
            Larsson,E.
  TITLE     Accurate mapping of mitochondrial DNA deletions and duplications
            using deep sequencing
  JOURNAL   PLoS Genet 16 (12), e1009242 (2020)
   PUBMED   33315859
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2711)
  AUTHORS   Matic,S., Jiang,M., Nicholls,T.J., Uhler,J.P.,
            Dirksen-Schwanenland,C., Polosa,P.L., Simard,M.L., Li,X.,
            Atanassov,I., Rackham,O., Filipovska,A., Stewart,J.B.,
            Falkenberg,M., Larsson,N.G. and Milenkovic,D.
  TITLE     Mice lacking the mitochondrial exonuclease MGME1 accumulate mtDNA
            deletions without developing progeria
  JOURNAL   Nat Commun 9 (1), 1202 (2018)
   PUBMED   29572490
  REMARK    GeneRIF: MGME1 is part of a termination complex acting at the end
            of the D-loop region where it modulates mtDNA replication and
            H-strand transcription termination. Mice with MGME1 deficiency
            accumulate a long linear subgenomic mtDNA species, but do not
            develop progeria.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2711)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL808123.9.
            
            On Sep 23, 2017 this sequence version replaced XM_006500302.2.
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 1. All four variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1059005.1,
                                           SRR1660813.179174.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                AL808123.9         10337-10380
            45-614              AL808123.9         11348-11917
            615-831             AL808123.9         15748-15964
            832-964             AL808123.9         18427-18559
            965-2711            AL808123.9         18914-20660
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 70.98 cM"
     gene            1..2711
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="mitochondrial genome maintenance exonuclease 1"
                     /db_xref="GeneID:74528"
                     /db_xref="MGI:MGI:1921778"
     exon            1..44
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            45..614
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1126
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="mitochondrial genome maintainance exonuclease 1"
                     /codon_start=1
                     /product="mitochondrial genome maintenance exonuclease 1"
                     /protein_id="NP_001342617.1"
                     /db_xref="CCDS:CCDS16816.1"
                     /db_xref="GeneID:74528"
                     /db_xref="MGI:MGI:1921778"
                     /translation="MKLPLTFCRLLSRLNRFSVKASPPVSFSTFSYLCSQKKKNSYEA
                     VDQAKYSRLVRSVLSRGPAQTPESLFKEDDVLYGPVSKHKAAEPEPQARVPQHCFPIF
                     NEERTGKPHTDASSSPLKIPLQRNSIPSVTRILQQTMPPEQSFFLERWKERMVLELGE
                     DGFAEYTSNVFLQGKQFHKALESILSPQENLTGGEEHPQCGYIESIQHILTEISGVQA
                     LESAVQHEALKYVGLLDCVAEYRGKLCVIDWKTSEKPKPLIRNTYDNPLQVVAYMGAV
                     NHDAHYSFQVQCGLIVVAYKDGSPAHPHFMDEELCSKYWAKWLLRLEEYTEKQKNLSA
                     PEPA"
     exon            615..831
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            832..964
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            965..2711
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1927..1932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1948
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="major polyA site"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1999
                     /gene="Mgme1"
                     /gene_synonym="8430406I07Rik"
                     /note="major polyA site"
ORIGIN      
        1 ccgcgcttct gcttcggggg cgtggagggg atggcgcctc acagatacga acagaacaca
       61 cttttaaact ttccagacta gagaaaagtt agaggggcga agtctaaaaa tgaaactgcc
      121 tctgaccttc tgcaggctgc ttagcaggtt aaacagattt tctgtaaaag caagccctcc
      181 tgtgagtttc tccactttct cttatttgtg tagccagaag aagaaaaact cttacgaagc
      241 agtagaccaa gcgaagtact ctcgtttagt acgctctgtc ttgtccagag gcccggccca
      301 gactccagag tcgttgttca aggaagatga tgtactgtat ggaccagtga gtaagcataa
      361 ggctgcagag ccggagccac aggccagagt cccacaacac tgctttccta tcttcaatga
      421 agagagaacc gggaaaccac acacagatgc ttcttcaagc cctttgaaga tccctttgca
      481 aaggaactcg atacccagtg tgacccgcat ccttcagcag accatgccac ctgaacagag
      541 cttctttttg gagaggtgga aagagcggat ggttctggag ctgggagaag acgggtttgc
      601 agaatatact tcaaatgtgt ttttacaagg caaacagttc cataaagcct tggaaagcat
      661 actgtcaccc caggagaacc taacaggggg agaagagcac ccccagtgtg gctacatcga
      721 aagcatccag catattctga cagaaatcag tggtgtgcaa gctctggaga gtgccgtcca
      781 gcatgaggcc ttgaagtatg tagggctgct ggactgtgtg gctgagtacc ggggcaagct
      841 gtgtgtgatt gattggaaga catcagaaaa accaaaacct ttaattcgaa atacatatga
      901 caacccgctg caagttgtgg cgtacatggg tgccgtaaac catgatgccc actacagttt
      961 tcaggttcag tgtggattaa ttgtggtggc ctataaggat gggtcccctg cgcaccctca
     1021 cttcatggat gaagagctct gttccaagta ttgggccaag tggcttctcc gactagaaga
     1081 atatacagaa aagcaaaaga acctgagcgc tccagagcca gcgtagaggc ccgggattgc
     1141 ttggcgatgc tgcgcggcga tctctgtttg ggagatgcac tgctgtccac tgagtttcta
     1201 aagaggaatg cggggaactg agggctacat taacacaagc tgaaggtgtg gtgggttggg
     1261 atgtacccac cgagaagcca gagagtctgg aggtcagcgc ctgactcacc agctgcacga
     1321 cctctggctg tttctcacct gagaggagga cactgggatt ggcagctgcc cagggacttc
     1381 tgggctccct tgcaaacctc tgtgtccctg gcaggatgcc cagtctttga attgagatca
     1441 gaaggccaaa tgaagcatca tgtgccacac atgtccctag agttctcagg gatgggaggg
     1501 gccctgcacc ctcagggtat acagagagag agagagtgag caagcaagca cgtaggcatc
     1561 gccaccacag tttcctagaa ggtgttttat acactctgtg tcattaggaa aaacttattt
     1621 cagcctaagg aagcctggct gcctagagag ctggggcagg caatacatca ttggctaggt
     1681 ccttttcctt accagagaag ccccagcagc aggaagcctg agtgtggaag tcaccgtcgc
     1741 tagggtttgg gtatctattt ctggtgtcag caggccatgg actacaaaaa atactgttgt
     1801 ggaacttgtt aacaaagagg aacaatggta aaactttttt tctgtgtttg ataagtaaca
     1861 acaaaaaaaa aatcattggc tgttcttggg agacggtttt ctgttttata aagtaaatct
     1921 agtagcatta aaattataag ttacacaatc acatttagtt ttcattagtc ttaaaaaaaa
     1981 ataaaagata tttaactgat tcctagtctt tcagtggacc agagttctgt tcccagtacc
     2041 catgtctggc agctcacatt gtctatgagg tcagttccgg gggacccagc acctcttctg
     2101 gccttttgag gactcgtgca cagacataca tccacatgaa tagtgagctt ttttttctga
     2161 cttctctcat ctttatttat tttctcacat gttacaccct aaccagtttc ccatccctcc
     2221 tctcctccca gttcctcccc cagcctctca cctcttctct ccccctggtt cactcctccc
     2281 cagttcccct tcagaaaagg gcaggcctcc cggggatatc aacctaacat gttatatcaa
     2341 gttgcaataa gactaggcac ctccctcata tgaagcaagc cagtaggagg aaaggggtcc
     2401 caaaagcagg caaaagagtc agagacagcc cctgctccca ctgttagggg tcccacaaga
     2461 ggaccaagtt acccagtcct aacacagatg cagggactta ggtcaaaccg gttcacgctg
     2521 cctgactgcc ggttctgtct cactgagccc ctatgagtcc tgcctggtta gttgattctc
     2581 tgggcttttt tgtggtgttc atgaccccta tggctcctac aatactctct cctgcctctt
     2641 ctacagtatt ctctgagcaa aaatgagctt atttaaaaga aaaggtaata aagcatttta
     2701 tttgtttttg t
//
