LOCUS       NM_172948               4277 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus mannoside acetylglucosaminyltransferase 5, isoenzyme B
            (Mgat5b), mRNA.
ACCESSION   NM_172948 XM_001003677 XM_001003684 XM_001003690
VERSION     NM_172948.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Nagai,K., Muto,Y., Miura,S., Takahashi,K., Naruse,Y., Hiruta,R.,
            Hashimoto,Y., Uzuki,M., Haga,Y., Fujii,R., Ueda,K., Kawaguchi,Y.,
            Fujii,M. and Kitazume,S.
  TITLE     Brain-specific glycosylation enzyme GnT-IX maintains levels of
            protein tyrosine phosphatase receptor PTPRZ, thereby mediating
            glioma growth
  JOURNAL   J Biol Chem 299 (9), 105128 (2023)
   PUBMED   37543361
  REMARK    GeneRIF: Brain-specific glycosylation enzyme GnT-IX maintains
            levels of protein tyrosine phosphatase receptor PTPRZ, thereby
            mediating glioma growth.
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Xie,W.R., Jen,H.I., Seymour,M.L., Yeh,S.Y., Pereira,F.A.,
            Groves,A.K., Klisch,T.J. and Zoghbi,H.Y.
  TITLE     An Atoh1-S193A Phospho-Mutant Allele Causes Hearing Deficits and
            Motor Impairment
  JOURNAL   J Neurosci 37 (36), 8583-8594 (2017)
   PUBMED   28729444
REFERENCE   3  (bases 1 to 4277)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   4  (bases 1 to 4277)
  AUTHORS   Kizuka,Y., Kitazume,S., Okahara,K., Villagra,A., Sotomayor,E.M. and
            Taniguchi,N.
  TITLE     Epigenetic regulation of a brain-specific glycosyltransferase
            N-acetylglucosaminyltransferase-IX (GnT-IX) by specific chromatin
            modifiers
  JOURNAL   J Biol Chem 289 (16), 11253-11261 (2014)
   PUBMED   24619417
  REMARK    GeneRIF: a new epigenetic mechanism of brain-specific GnT-IX
            expression regulated by defined chromatin modifiers, providing new
            insights into the tissue-specific expression of
            glycosyltransferases.
REFERENCE   5  (bases 1 to 4277)
  AUTHORS   Henion,T.R. and Schwarting,G.A.
  TITLE     N-linked polylactosamine glycan synthesis is regulated by
            co-expression of beta3GnT2 and GCNT2
  JOURNAL   J Cell Physiol 229 (4), 471-478 (2014)
   PUBMED   24105809
REFERENCE   6  (bases 1 to 4277)
  AUTHORS   Lee,J.K., Matthews,R.T., Lim,J.M., Swanier,K., Wells,L. and
            Pierce,J.M.
  TITLE     Developmental expression of the neuron-specific
            N-acetylglucosaminyltransferase Vb (GnT-Vb/IX) and identification
            of its in vivo glycan products in comparison with those of its
            paralog, GnT-V
  JOURNAL   J Biol Chem 287 (34), 28526-28536 (2012)
   PUBMED   22715095
  REMARK    GeneRIF: Developmental expression of the neuron-specific
            N-acetylglucosaminyltransferase Vb (GnT-Vb/IX) and identification
            of its in vivo glycan products in comparison with those of its
            paralog, GnT-V.
REFERENCE   7  (bases 1 to 4277)
  AUTHORS   Kizuka,Y., Kitazume,S., Yoshida,M. and Taniguchi,N.
  TITLE     Brain-specific expression of N-acetylglucosaminyltransferase IX
            (GnT-IX) is regulated by epigenetic histone modifications
  JOURNAL   J Biol Chem 286 (36), 31875-31884 (2011)
   PUBMED   21771782
  REMARK    GeneRIF: revealed that the genomic region around the
            transcriptional start site of the acetylglucosaminyltransferase IX
            gene was highly associated with active chromatin histone marks in a
            neural cell-specific manner.
REFERENCE   8  (bases 1 to 4277)
  AUTHORS   Faux,C., Rakic,S., Andrews,W., Yanagawa,Y., Obata,K. and
            Parnavelas,J.G.
  TITLE     Differential gene expression in migrating cortical interneurons
            during mouse forebrain development
  JOURNAL   J Comp Neurol 518 (8), 1232-1248 (2010)
   PUBMED   20151419
REFERENCE   9  (bases 1 to 4277)
  AUTHORS   Kawaguchi,A., Ikawa,T., Kasukawa,T., Ueda,H.R., Kurimoto,K.,
            Saitou,M. and Matsuzaki,F.
  TITLE     Single-cell gene profiling defines differential progenitor
            subclasses in mammalian neurogenesis
  JOURNAL   Development 135 (18), 3113-3124 (2008)
   PUBMED   18725516
REFERENCE   10 (bases 1 to 4277)
  AUTHORS   Inamori,K., Mita,S., Gu,J., Mizuno-Horikawa,Y., Miyoshi,E.,
            Dennis,J.W. and Taniguchi,N.
  TITLE     Demonstration of the expression and the enzymatic activity of
            N-acetylglucosaminyltransferase IX in the mouse brain
  JOURNAL   Biochim Biophys Acta 1760 (4), 678-684 (2006)
   PUBMED   16413118
  REMARK    GeneRIF: GnT-IX transcripts were restricted to the cerebrum,
            cerebellum, thymus and testis, whereas GnT-V transcripts were
            expressed ubiquitously in mouse tissues
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC094604.1.
            
            On or before May 4, 2006 this sequence version replaced
            XM_001003690.1, XM_001003684.1, XM_001003677.1, NM_172948.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094604.1, AB119127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 81.49 cM"
     gene            1..4277
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /note="mannoside acetylglucosaminyltransferase 5,
                     isoenzyme B"
                     /db_xref="GeneID:268510"
                     /db_xref="MGI:MGI:3606200"
     exon            1..681
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     CDS             614..2992
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /EC_number="2.4.1.155"
                     /note="N-acetylglucosaminyltranferase VB;
                     UDP-N-acetylglucosamine: alpha1,6-D-mannoside
                     beta1,6-N-acetylglucosaminyltransferase b;
                     beta1,6-N-acetylglucosaminyltransferase IX (GnT-IX);
                     GNT-Vb; glcNAc-T Vb; N-acetylglucosaminyl-transferase Vb;
                     mannoside acetylglucosaminyltransferase 5B;
                     alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
                     B"
                     /codon_start=1
                     /product="alpha-1,6-mannosylglycoprotein
                     6-beta-N-acetylglucosaminyltransferase B"
                     /protein_id="NP_766536.2"
                     /db_xref="CCDS:CCDS25682.1"
                     /db_xref="GeneID:268510"
                     /db_xref="MGI:MGI:3606200"
                     /translation="MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQ
                     FSARRLGDSPFTIRTEVPGSPESRGALRKMSDLLELMVKRMDMLARLENSSELHRTAS
                     VAHLAADRLTPGASLIERIQAIAQNVSDIAVKVDQILRHSLILHSKVSEGRRDQCEAP
                     SDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPPLPWRNQTA
                     ARTAPKSLPRVQAVFRSNLSHLLELMGSGKESLIFMKKRTRRFTAQWTKAAKYLAQKL
                     GDIRRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILAALYVLGHSLRIT
                     VSLKELQSNLGVPPGRGNCPLTVPLPFDLIYTDYHGLQQMKQHMGLSFKKYRCRIRVI
                     DTFGTEPAYNHEEYATLHGYRTNWGYWNLNPKQFMTMFPHTPDNSFMGFVSEELNETE
                     KQLIKDGKASNMAVVYGKEASIWKLQGKEKFLAVLNKYMEIHGTVYYESQRPPEVPAF
                     VKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCIFLQSRFSPPHSSLNH
                     EFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSDEFETAIKAIMNTQVDPYLPYE
                     YTCAGMLERINAYIQHQDFCVGPSPLPPGASTAQSPFVLAPNATHLEWAQNISSVPGA
                     WPPTHSLRAWLAAPGRACTDACLDHGLICEPSFFPFLNSQNSFLKLQVPCDSTEWEMH
                     HLYPAFAQPGQECYLQKEPLLFSCAGASTKYQRLCPCRDFRKGQVALCQGCL"
     exon            682..794
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            795..942
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1058
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1132
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..1303
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1468
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1638
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1639..1770
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1904
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2035
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..2041
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2197
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2344
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2461
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2462..2536
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2793
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     exon            2794..4254
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4222..4227
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /note="hexamer: AATAAA"
     polyA_site      4254
                     /gene="Mgat5b"
                     /gene_synonym="C330018B01; GnT-IX; mGnTVB"
                     /note="major polyA site"
ORIGIN      
        1 gcggcgcctc gggttgtagc gccgcgcggg agcccggagc ccggagcgcg ggtggccgag
       61 cgaccgagcg ggcgagcggg cgggcgagga cgaaggcagg cgagcgcgca gccgttcctt
      121 cccaaagcca tgggcgcggc gctgcgcgcg gctgcagagc ggcgcggccg gaaggtgcgg
      181 ggggccgggg tgcgggcctg gggccgcctc tagccggctc tgagggcgcg ggccccggga
      241 atgagggcgc ccgccgcggg aagcccgttt gcgcgccgcg gcgccgtccc gcccagccag
      301 cgagcctagc aggcagacgc gcggccggcg atctgggggc gcgccgcctc gccttcccca
      361 aaatgtgaat cggggagggc ggagacgcag agagcgcccg gccccaagct ctcgccgaac
      421 ccctgccctg cgcgcccagg ccgcgccgtg ccccgcgcgg ggctgcagag ccaccgtgcc
      481 ccgcgctccc tcggtgctgc gaccccccgg cttcggcccg cagcggcttc gtggttcccg
      541 aggcggtcag agccgggccc aggacggtgc gtccggcctc gcccccggct tctcgcccag
      601 acaagtttga acaatgatca cagtcaaccc agatgggaag ataatggtca gaagatgcct
      661 ggtcaccctg agaccctttc ggctgtttgt cctgggcatc ggcttcttca ctctctgctt
      721 cctgatgaca tctttgggag gccagttctc tgcccggcgc ctgggggact cgcccttcac
      781 catccgcaca gaagtgccag gcagcccaga gtcacgtggt gcccttcgca agatgagcga
      841 cctgctggag ctgatggtga agcgcatgga tatgctggcc aggctggaga atagcagcga
      901 gctgcaccgg actgccagtg tggcgcactt agccgcagac aggctcaccc ctggggccag
      961 cctcattgaa aggatccagg ccattgccca gaatgtgtct gacatcgctg tgaaggtgga
     1021 ccagatcctg cgccacagcc tgattctgca tagcaaggtg tctgaaggtc ggagggacca
     1081 gtgtgaagca cccagtgacc ccaagttccc tgactgttcc gggaaagtgg agtggatgcg
     1141 cgcccgctgg acctctgacc cctgctacgc cttctttgga gtagacggca ctgagtgctc
     1201 cttcctcatc tacctcagtg aggttgagtg gttctgtccc ccgttgccct ggaggaacca
     1261 gacagctgcc cggacagccc ccaagtccct tcccagagtc caggctgtgt tccgaagcaa
     1321 cctgtcccac ctcctggagc tgatgggcag tgggaaggag tccctcatct tcatgaagaa
     1381 gcgaaccagg cggttcaccg cacagtggac caaggctgcc aagtacctgg cacagaagct
     1441 gggggacatt cggagggacc agaagcaaat ccttgtccac attggcttcc tgacagagga
     1501 gtctggggac gtgttcagcc caagggtact gaagggcggg cctctgggag agatggtaca
     1561 gtgggcagac atcctggctg ctctctacgt gctgggccat agcctgcgga tcacagtctc
     1621 cctgaaggag ctgcagagta acttaggggt gccgccaggc cgggggaact gcccactcac
     1681 cgtacctctg ccttttgacc tcatctacac ggactatcac ggcttgcagc agatgaaaca
     1741 gcacatggga ctgtccttca agaagtaccg gtgcagaatc cgagtcatcg acacctttgg
     1801 gacggagcca gcgtacaacc acgaggagta tgccacgctg cacggctacc ggaccaactg
     1861 gggttactgg aacctcaacc ccaagcagtt catgaccatg ttccctcaca ccccagacaa
     1921 ctccttcatg ggcttcgtgt ccgaggagct caatgagacc gagaagcagc tcatcaaaga
     1981 tggcaaggcc agcaacatgg cggtggtgta cggcaaggag gcgagtatct ggaagctcca
     2041 gggcaaggag aagttcctgg ccgtcctcaa caagtacatg gagatccacg gtaccgtgta
     2101 ctatgagagc cagcggccac ccgaggtccc cgccttcgtg aagaaccacg gcctcctacc
     2161 gcagcctgag ttccagcagc tgctgcggaa ggccaagctc tttatagggt tcggattccc
     2221 ctacgagggc ccagcaccgt tggaagccat tgccaatggc tgcatcttcc tacagtctcg
     2281 cttcagcccg ccccacagct ccctcaacca cgagttcttc cggggcaagc ccacctccag
     2341 ggaggtgttc tcccagcatc cgtatgcaga gaactttatt ggcaagccgc acgtgtggac
     2401 cgtggactat aacaactccg atgagtttga aacagccatt aaggccatca tgaacaccca
     2461 ggtagaccca tatctgccct atgaatatac ctgtgcaggg atgctggaac ggatcaatgc
     2521 ctacatccaa caccaggact tctgtgtggg tccaagccct cttccaccag gggccagcac
     2581 tgcccagagt ccatttgtct tagctcctaa tgcaactcat ctcgagtggg cccagaacat
     2641 cagctcagtt ccgggagcct ggccccctac ccactctctg cgggcctggc tggcagcccc
     2701 tggaagggcc tgcacggacg cctgcctgga ccatggattg atctgcgagc cttccttctt
     2761 ccctttcctc aacagccaga attcgttcct caagctgcag gtgccctgtg acagcactga
     2821 gtgggagatg catcacttgt accctgcctt tgcccaaccc ggccaagagt gctacctaca
     2881 aaaagagcca ctgctcttca gctgtgctgg tgccagcacc aagtaccaga ggctctgccc
     2941 ctgccgtgac ttccgcaagg gtcaggtggc cttgtgccag ggctgcctgt gaggccggag
     3001 ccaccctgcc cagaacctgc ccacccgcac gtggttggca agcaccagca ctttctgagc
     3061 tccggtcacg ctcactacgt gtcccctggc tgcagcctcc cctggccagg gatgggaaga
     3121 ggaagctgag gagacagcag ctccaggcct gcagctccct cctaggggct tccttgcctc
     3181 gccataggac ctgaggccaa gcatgtgggc tgacctccct gtcgggtgta cccaggagca
     3241 cgtggatgga gatccctggc tttctgaggt ctggaccagc tggagatgtg gccttgacca
     3301 tgcttggacc cagcataggc cttttgatcc acaaggctgg gagcatggcc atgccgcccc
     3361 ctattcacca gaggtctcaa gggataggga acaggtcaca gccacacttg ctgtgagggc
     3421 cacaccctca catgaggcaa cagttcacgc agggccagtc cagcctcctc agttgcttgg
     3481 gggggggggg gaacgacaaa gggacagaga gctcagggag gctagtgccc ctccctgttg
     3541 ctcaaccctg cttcctccag cagacttccc tctgggcctc tcctgacacc cagttctggc
     3601 atggcctgtg actggtccac agccttggtc tctgcccctc tggtggccac ctccacccta
     3661 gcttctgaca acaggtccca ggccccacca aggcctcctc tgcagactct gacaacattt
     3721 ccaaccttcg agcatctcat gccggccagg ctgggatgaa gtttcaccca caatacacta
     3781 ggctggttct gatacgcagg tccatcagac ctcccctggc tcccaccctt ctgggcccca
     3841 tacttgcccc tcccttctct ttcctccagc ctcaggccac ttcagtacac tcagacttca
     3901 gtacccatct ctgtggcatg ccctcctcca gtcctgggtc acccttccct ctcccacccc
     3961 agggccagcc aggttccagg aacactgcca ttgctcatat atggcccgct cccaggcccc
     4021 ctgggaccag acagttgtag gaaggaactt tcctgcgtgg gacttgccag tgtcccctgt
     4081 cctggttggc ccctgtctgt ggcagcagaa tccagagcaa caagagacca gtggttccga
     4141 ggatctgcct gtcggaggaa agcaatagag acacttatct ctctcttctc tctctctttt
     4201 ttttaaagat ttatttcttg aaataaatat tttattggga tgtgagatga tgaaaaaaaa
     4261 aaaaaaaaaa aaaaaaa
//
