LOCUS       NM_145128               3104 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus mannoside acetylglucosaminyltransferase 5 (Mgat5),
            mRNA.
ACCESSION   NM_145128 XM_358334
VERSION     NM_145128.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Hollander,E.E., Flock,R.E., McDevitt,J.C., Vostrejs,W.P.,
            Campbell,S.L., Orlen,M.I., Kemp,S.B., Kahn,B.M., Wellen,K.E.,
            Kim,I.K. and Stanger,B.Z.
  TITLE     N-glycosylation by Mgat5 imposes a targetable constraint on
            immune-mediated tumor clearance
  JOURNAL   JCI Insight 9 (12), e178804 (2024)
   PUBMED   38912584
  REMARK    GeneRIF: N-glycosylation by Mgat5 imposes a targetable constraint
            on immune-mediated tumor clearance.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Liu,X.P., Li,J.Q., Li,R.Y., Cao,G.L., Feng,Y.B. and Zhang,W.
  TITLE     Loss of N-acetylglucosaminyl transferase V is involved in the
            impaired osteogenic differentiation of bone marrow mesenchymal stem
            cells
  JOURNAL   Exp Anim 72 (3), 413-424 (2023)
   PUBMED   37019682
  REMARK    GeneRIF: Loss of N-acetylglucosaminyl transferase V is involved in
            the impaired osteogenic differentiation of bone marrow mesenchymal
            stem cells.
REFERENCE   3  (bases 1 to 3104)
  AUTHORS   Yale,A.R., Kim,E., Gutierrez,B., Hanamoto,J.N., Lav,N.S.,
            Nourse,J.L., Salvatus,M., Hunt,R.F., Monuki,E.S. and Flanagan,L.A.
  TITLE     Regulation of neural stem cell differentiation and brain
            development by MGAT5-mediated N-glycosylation
  JOURNAL   Stem Cell Reports 18 (6), 1340-1354 (2023)
   PUBMED   37172586
  REMARK    GeneRIF: Regulation of neural stem cell differentiation and brain
            development by MGAT5-mediated N-glycosylation.
REFERENCE   4  (bases 1 to 3104)
  AUTHORS   Cawley,N.X., Lyons,A.T., Abebe,D., Luke,R., Yerger,J., Telese,R.,
            Wassif,C.A., Bailey-Wilson,J.E. and Porter,F.D.
  TITLE     Complex N-Linked Glycosylation: A Potential Modifier of
            Niemann-Pick Disease, Type C1 Pathology
  JOURNAL   Int J Mol Sci 23 (9), 5082 (2022)
   PUBMED   35563467
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3104)
  AUTHORS   Sy,M., Brandt,A.U., Lee,S.U., Newton,B.L., Pawling,J., Golzar,A.,
            Rahman,A.M.A., Yu,Z., Cooper,G., Scheel,M., Paul,F., Dennis,J.W.
            and Demetriou,M.
  TITLE     N-acetylglucosamine drives myelination by triggering
            oligodendrocyte precursor cell differentiation
  JOURNAL   J Biol Chem 295 (51), 17413-17424 (2020)
   PUBMED   33453988
REFERENCE   6  (bases 1 to 3104)
  AUTHORS   Demetriou,M., Granovsky,M., Quaggin,S. and Dennis,J.W.
  TITLE     Negative regulation of T-cell activation and autoimmunity by Mgat5
            N-glycosylation
  JOURNAL   Nature 409 (6821), 733-739 (2001)
   PUBMED   11217864
REFERENCE   7  (bases 1 to 3104)
  AUTHORS   Granovsky,M., Fata,J., Pawling,J., Muller,W.J., Khokha,R. and
            Dennis,J.W.
  TITLE     Suppression of tumor growth and metastasis in Mgat5-deficient mice
  JOURNAL   Nat Med 6 (3), 306-312 (2000)
   PUBMED   10700233
REFERENCE   8  (bases 1 to 3104)
  AUTHORS   Dennis,J.W., Granovsky,M. and Warren,C.E.
  TITLE     Glycoprotein glycosylation and cancer progression
  JOURNAL   Biochim Biophys Acta 1473 (1), 21-34 (1999)
   PUBMED   10580127
  REMARK    Review article
REFERENCE   9  (bases 1 to 3104)
  AUTHORS   Granovsky,M., Fode,C., Warren,C.E., Campbell,R.M., Marth,J.D.,
            Pierce,M., Fregien,N. and Dennis,J.W.
  TITLE     GlcNAc-transferase V and core 2 GlcNAc-transferase expression in
            the developing mouse embryo
  JOURNAL   Glycobiology 5 (8), 797-806 (1995)
   PUBMED   8720078
REFERENCE   10 (bases 1 to 3104)
  AUTHORS   Shoreibah,M., Perng,G.S., Adler,B., Weinstein,J., Basu,R.,
            Cupples,R., Wen,D., Browne,J.K., Buckhaults,P., Fregien,N. and
            Pierce,M.
  TITLE     Isolation, characterization, and expression of a cDNA encoding
            N-acetylglucosaminyltransferase V
  JOURNAL   J Biol Chem 268 (21), 15381-15385 (1993)
   PUBMED   8340368
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY752687.1, AK030402.1 and BC125533.1.
            
            On Feb 27, 2007 this sequence version replaced NM_145128.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030402.1, SRR7345562.1307508.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-492               BY752687.1         1-492
            493-2658            AK030402.1         461-2626
            2659-3104           BC125533.1         2067-2512
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 55.71 cM"
     gene            1..3104
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /note="mannoside acetylglucosaminyltransferase 5"
                     /db_xref="GeneID:107895"
                     /db_xref="MGI:MGI:894701"
     exon            1..442
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            443..492
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            493..875
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     CDS             635..2857
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /EC_number="2.4.1.155"
                     /note="GNT-V; glcNAc-T V; N-acetylglucosaminyl-transferase
                     V; alpha-mannoside
                     beta-1,6-N-acetylglucosaminyltransferase;
                     beta1,6N-acetylglucosaminyltransferase V"
                     /codon_start=1
                     /product="alpha-1,6-mannosylglycoprotein
                     6-beta-N-acetylglucosaminyltransferase A"
                     /protein_id="NP_660110.2"
                     /db_xref="CCDS:CCDS15245.1"
                     /db_xref="GeneID:107895"
                     /db_xref="MGI:MGI:894701"
                     /translation="MAFFSPWKLSSQKLGFFLVTFGFIWGMMLLHFTIQQRTQPESSS
                     MLREQILDLSKRYIKALAEENRDVVDGPYAGVMTAYDLKKTLAVLLDNILQRIGKLES
                     KVDNLVNGTGANSTNSTTAVPSLVSLEKINVADIINGVQEKCVLPPMDGYPHCEGKIK
                     WMKDMWRSDPCYADYGVDGTSCSFFIYLSEVENWCPRLPWRAKNPYEEADHNSLAEIR
                     TDFNILYGMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKILVHLGLL
                     TKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNR
                     SGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHASYAQ
                     SKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLV
                     YGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRET
                     KLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYA
                     EVFIGRPHVWTVDLNNREEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDF
                     CHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKEKDLLKYKVTCQS
                     SELYKDILVPSFYPKSKHCVFQGDLLLFSCAGAHPTHQRICPCRDFIKGQVALCKDCL
                     "
     exon            876..1037
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1114
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1204
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1276
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1438
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1608
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1743
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1744..1877
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..2011
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2012..2161
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2308
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2425
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2500
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2658
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..3104
                     /gene="Mgat5"
                     /gene_synonym="4930471A21Rik; 5330407H02Rik; GlcNAc-TV"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggaacgtccg tggcccggaa tccctttgcc ttggagagcg gcgcgagcct agaagctgag
       61 agaagttggg taggtcgatc cgggaagggt agcagaaacc tcgaaaagat ctcctaacca
      121 ttctgtcctg atcgtttgga ctcaggagga acgtccctgc ttgcgtctag gggcggaagg
      181 tggacgcgct gcgcacggcc ccgacagggc tggtagtttt cagaccgtcc ctgccgaggc
      241 ccgctcgccc ggcagccctg cggctcccgc ggtggcggcg gcggccccag gctcgatcta
      301 gcccaggtag ccgcatctca gtctcagggg gcgctctgac cccgctcctg gctgtgcctg
      361 ggacctctgt tcctggagca aggctgctgg agagtgaacc cgactgctac tgatggtgct
      421 tctgctgctc ccctactagc aggagtgact cctacccaaa agtggacttt ggaggagggt
      481 ccattaggtc atcagaatgg aagcccagca aggaaaccag ctgactcagg agccggagtg
      541 agagccacgc actctccgcc ccaacctgca ccatgaactt gccttcccct tctgcttgtt
      601 gagagccaag ggaatggtac attgctagag agagatggct ttcttttctc cctggaagtt
      661 gtcctctcag aagctgggct ttttcctggt gactttcggc ttcatctggg gcatgatgct
      721 tctgcacttc accatccagc agcggactca gcccgagagc agctccatgt tacgggagca
      781 gatccttgac ctcagcaaga ggtacattaa ggcactggca gaggagaaca gggacgtggt
      841 ggatggcccc tacgctggtg tcatgacagc ctatgatctg aagaaaacgc tcgccgtctt
      901 gctggataac atcctgcagc gcattggcaa gctcgagtca aaggtggaca atctggtcaa
      961 cggcacagga gcgaactcca ccaactccac cacggctgtc cccagcttgg tgtcgcttga
     1021 gaaaattaat gtggcagata tcattaatgg agttcaggaa aaatgtgtat tgcctcctat
     1081 ggatggctac ccccactgcg aggggaaaat caagtggatg aaggacatgt ggcgctcgga
     1141 cccctgctac gcagactatg gagtggacgg gacctcctgc tcctttttta tttacctcag
     1201 tgaggttgaa aattggtgtc ctcgtttacc ttggagagca aaaaatccct atgaagaagc
     1261 tgatcataac tcattggcgg aaatccgtac ggattttaac attctctacg gcatgatgaa
     1321 gaagcacgag gagttccgtt ggatgaggct tcggatccgg cgaatggctg acgcgtggat
     1381 ccaagctatc aagtctctgg cggagaaaca aaaccttgag aagaggaaac ggaagaaaat
     1441 ccttgttcac ctggggctcc tgaccaagga atcgggcttc aagattgcgg agacagcatt
     1501 cagcggtggc cctctgggtg aactcgttca gtggagtgac ttaatcacat ctctgtacct
     1561 gctgggccat gacatccgga tctcggcctc actggctgag ctcaaggaga taatgaagaa
     1621 ggttgttgga aaccggtctg gctgtccaac tgtaggagac agaatcgttg agctgattta
     1681 tatcgatatt gtgggacttg ctcaatttaa gaaaacacta gggccatcct gggttcatta
     1741 ccagtgcatg ctccgggtgc tagactcctt tggaacagaa cctgagttca atcatgcgag
     1801 ctatgcccag tcaaaaggcc acaagacccc ctggggaaag tggaatctga acccgcagca
     1861 gttttacacc atgttccctc ataccccaga caacagcttt ctgggcttcg tggtggagca
     1921 gcacctgaac tccagcgaca ttcaccacat caacgagatc aaaaggcaga accagtccct
     1981 tgtgtatggc aaagtggata gtttctggaa gaataagaaa atctacctgg atatcattca
     2041 cacgtacatg gaagtgcacg ccactgttta tggctccagt accaagaaca ttcccagtta
     2101 cgtgaaaaac catggcattc tcagtggacg tgacctgcag tttcttctcc gggaaaccaa
     2161 gctgttcgtt gggctcggat tcccttatga aggcccagct cccctggagg ccatcgcgaa
     2221 tggatgtgct ttcctgaacc ccaagttcaa ccctcccaaa agcagcaaaa acacagactt
     2281 cttcattggc aagccaacac tgagagagct gacatcccag catccttacg cagaagtctt
     2341 catcggccgg ccacacgtct ggactgtgga tctcaataac cgagaggaag tagaagatgc
     2401 agtaaaagcc atcttaaacc agaagattga gccgtatatg ccatatgagt tcacatgtga
     2461 aggcatgctg cagagaatca acgctttcat tgaaaaacag gacttctgcc atggccaagt
     2521 gatgtggccg cccctcagcg ccctgcaggt gaagctggct gagccagggc agtcctgcaa
     2581 acaggtgtgc caggagagcc agctcatctg cgagccttcc ttctttcaac acctcaacaa
     2641 ggaaaaggac ctgctgaagt ataaggtgac ctgccaaagc tcagaactgt acaaggacat
     2701 cctggtgccc tccttctacc ccaagagcaa gcactgtgtg ttccaagggg acctcctgct
     2761 cttcagttgt gccggagccc accccacaca ccagcggatc tgcccctgcc gggacttcat
     2821 caagggccaa gtggccctct gcaaagactg cctatagcat cgctgccctg aattaactca
     2881 gacgggaaag acgtggctcc actgggcagg gccaaggggc acaaagacat tcagggactc
     2941 tgaccagagc ctgagatctt tggtccaggg cttgagttta gtaccgctcc agccacagcc
     3001 agtgcatccc agtttacacc aaaaccacaa gggaacaggt tagaacagga acctgggttc
     3061 tcctcaatgt aaggaatgtc ctctctgtct gggagatcga gcga
//
