LOCUS       NM_146035               2620 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus mannoside acetylglucosaminyltransferase 2 (Mgat2),
            mRNA.
ACCESSION   NM_146035
VERSION     NM_146035.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Asmat,M.S., Zheng,X.Y., Nauman,M., Zheng,D. and Stanley,P.
  TITLE     Deletion of Mgat2 in spermatogonia blocks spermatogenesis
  JOURNAL   Front Cell Dev Biol 12, 1428715 (2024)
   PUBMED   39364139
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2620)
  AUTHORS   Mortales,C.L., Lee,S.U., Manousadjian,A., Hayama,K.L. and
            Demetriou,M.
  TITLE     N-Glycan Branching Decouples B Cell Innate and Adaptive Immunity to
            Control Inflammatory Demyelination
  JOURNAL   iScience 23 (8), 101380 (2020)
   PUBMED   32745987
REFERENCE   4  (bases 1 to 2620)
  AUTHORS   Mochida,T., Take,K., Maki,T., Nakakariya,M., Adachi,R., Sato,K.,
            Kitazaki,T. and Takekawa,S.
  TITLE     Inhibition of MGAT2 modulates fat-induced gut peptide release and
            fat intake in normal mice and ameliorates obesity and diabetes in
            ob/ob mice fed on a high-fat diet
  JOURNAL   FEBS Open Bio 10 (3), 316-326 (2020)
   PUBMED   31837122
REFERENCE   5  (bases 1 to 2620)
  AUTHORS   Qi,J., Lang,W., Connelly,M.A., Du,F., Liang,Y., Caldwell,G.W.,
            Martin,T., Hansen,M.K., Kuo,G.H., Gaul,M.D., Pocai,A. and Lee,S.
  TITLE     Metabolic tracing of monoacylglycerol acyltransferase-2 activity in
            vitro and in vivo
  JOURNAL   Anal Biochem 524, 68-75 (2017)
   PUBMED   27665677
  REMARK    GeneRIF: The use of 1-oleoyl-glycerol-d5 and (U13)C-TG oil followed
            by LC/ESI/MS/MS detection of stable-isotopic labeled DAG, TG, or
            glycerol provides a wide range of applications to study
            pathophysiological regulation of the monoacylglycerol pathway and
            MGAT2 activity.
REFERENCE   6  (bases 1 to 2620)
  AUTHORS   Yen,C.L. and Farese,R.V. Jr.
  TITLE     MGAT2, a monoacylglycerol acyltransferase expressed in the small
            intestine
  JOURNAL   J Biol Chem 278 (20), 18532-18537 (2003)
   PUBMED   12621063
  REMARK    GeneRIF: MGAT2 is a monoacylglycerol acyltransferase expressed in
            the small intestine
REFERENCE   7  (bases 1 to 2620)
  AUTHORS   Cao,J., Lockwood,J., Burn,P. and Shi,Y.
  TITLE     Cloning and functional characterization of a mouse intestinal
            acyl-CoA:monoacylglycerol acyltransferase, MGAT2
  JOURNAL   J Biol Chem 278 (16), 13860-13866 (2003)
   PUBMED   12576479
  REMARK    GeneRIF: MGAT2 may play an important role in dietary fat absorption
REFERENCE   8  (bases 1 to 2620)
  AUTHORS   Lowe,J.B. and Marth,J.D.
  TITLE     A genetic approach to Mammalian glycan function
  JOURNAL   Annu Rev Biochem 72, 643-691 (2003)
   PUBMED   12676797
  REMARK    Review article
REFERENCE   9  (bases 1 to 2620)
  AUTHORS   Wang,Y., Schachter,H. and Marth,J.D.
  TITLE     Mice with a homozygous deletion of the Mgat2 gene encoding
            UDP-N-acetylglucosamine:alpha-6-D-mannoside
            beta1,2-N-acetylglucosaminyltransferase II: a model for congenital
            disorder of glycosylation type IIa
  JOURNAL   Biochim Biophys Acta 1573 (3), 301-311 (2002)
   PUBMED   12417412
  REMARK    Review article
REFERENCE   10 (bases 1 to 2620)
  AUTHORS   Wang,Y., Tan,J., Sutton-Smith,M., Ditto,D., Panico,M.,
            Campbell,R.M., Varki,N.M., Long,J.M., Jaeken,J., Levinson,S.R.,
            Wynshaw-Boris,A., Morris,H.R., Le,D., Dell,A., Schachter,H. and
            Marth,J.D.
  TITLE     Modeling human congenital disorder of glycosylation type IIa in the
            mouse: conservation of asparagine-linked glycan-dependent functions
            in mammalian physiology and insights into disease pathogenesis
  JOURNAL   Glycobiology 11 (12), 1051-1070 (2001)
   PUBMED   11805078
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY101942.1, AK083370.1, AK152925.1 and AI595654.1.
            
            On Jun 2, 2007 this sequence version replaced NM_146035.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BY101942.1         1-44
            45-395              AK083370.1         2-352
            396-2543            AK152925.1         365-2512
            2544-2620           AI595654.1         1-77                c
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 28.73 cM"
     gene            1..2620
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /note="mannoside acetylglucosaminyltransferase 2"
                     /db_xref="GeneID:217664"
                     /db_xref="MGI:MGI:2384966"
     exon            1..2616
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /inference="alignment:Splign:2.1.0"
     CDS             497..1825
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /EC_number="2.4.1.143"
                     /note="alpha-1,6-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase; glcNAc-T II;
                     beta-1,2-N-acetylglucosaminyltransferase II;
                     N-glycosyl-oligosaccharide-glycoprotein
                     N-acetylglucosaminyltransferase II"
                     /codon_start=1
                     /product="alpha-1,6-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase precursor"
                     /protein_id="NP_666147.1"
                     /db_xref="CCDS:CCDS25947.1"
                     /db_xref="GeneID:217664"
                     /db_xref="MGI:MGI:2384966"
                     /translation="MRFRIYKRKVLILTLVVAACGFVLWSSNGRQRKSDALGPPLLDA
                     EPVRGAGHLAVSVGIRRVSNESAAPLVPAVPRPEVDNLTLRYRSLVYQLNFDQMLRNV
                     GNDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSLIS
                     RVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR
                     EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQ
                     ECPGCDVLSLGTYTTIRSFYGIADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC
                     TYDDYNWDWTLQYLTLACLPKIWKVLVPQAPRIFHAGDCGMHHKKTCRPSTQSAQIES
                     LLNSNKQYLFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRLQ"
     regulatory      2524..2529
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /note="hexamer: AATAAA"
     polyA_site      2549
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /note="major polyA site"
     regulatory      2586..2591
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
                     /note="hexamer: AATAAA"
     polyA_site      2613
                     /gene="Mgat2"
                     /gene_synonym="CDGS2; GLCNACTII; GNT-II; GNT2"
ORIGIN      
        1 ggagggtgtg ggccggaggg cccgggcgct cgcaggccag tcgcggcttg tcagagcggt
       61 cgctgccgga gcggggcgag gctgcggcgt tcggtgtcgc cgttccgccg tgcgagcaag
      121 gatgagcgcg cagaggacgc agagccacgg gcagcgcagg taaaggaatc acggtgtcgg
      181 cgaccgcggc ggggcttctt ccgggacccc cgggtgctga atggagagga ccgaggcgac
      241 gttgagccgc ggctcgtagc ggcggggcgg ctgccggagc tgcaactgcc agagaggatg
      301 cgcggagccc gggcggcgcg aggcagttga gagccttcgg gccccaggac gccggagccc
      361 gggatgagtt agcgagggcc gccgcggggg ccagttccta gggctgcagg ccaaggcgac
      421 ggcgccgccc gcccgcccct tccgtgcaga ggccgccagc ttcttttccg cgcccgcgct
      481 ccgggccctg gagaccatga ggttccgcat ctacaaacgg aaggtgctaa tcctgacgct
      541 cgtggtggcc gcctgcggct tcgtcctctg gagtagcaat gggcgacaaa ggaagagcga
      601 cgcccttggc ccgccgctgc tggacgcgga gcccgtacgg ggtgcgggcc accttgctgt
      661 gtccgtaggc atccgcaggg tctcaaacga atcggccgct cctctggttc ccgcggtccc
      721 gcggccggag gtggacaacc taacgctgcg gtaccggtcc ctggtgtacc agttgaactt
      781 cgatcagatg ctgaggaacg tcggtaatga cggcacctgg agccccgggg agctggtgct
      841 ggtggtccag gtgcataaca ggccggaata cctcaggttg ctgatagact cgcttcgcaa
      901 agcccagggt attcaggaag tcctagtcat ctttagccat gacttctggt ccgcagagat
      961 caacagtctg atctccaggg tggatttctg cccggttctg caagtgttct ttccgttcag
     1021 cattcagctg tacccgaatg agtttccggg tagtgaccct agagattgcc ccagagacct
     1081 aaagaagaat gcagctctga agttggggtg catcaatgcc gaataccctg actccttcgg
     1141 ccattacaga gaggccaagt tctcacaaac caaacaccat tggtggtgga agctgcattt
     1201 tgtatgggaa agagtcaaag ttcttcaaga ttacactggc cttatactct tcctggaaga
     1261 ggaccactac ttagccccag acttttacca tgtcttcaaa aagatgtgga aactgaagca
     1321 gcaggagtgt cctgggtgtg acgtcctctc tctagggacc tacaccacca ttcggagttt
     1381 ctatggcatt gctgacaaag tagatgtgaa aacttggaaa tcgacagagc acaacatggg
     1441 gctagccttg acccgagatg catatcagaa gcttatcgaa tgcacggaca ctttctgtac
     1501 ttacgatgat tataactggg actggactct tcagtatttg actctagctt gtcttcctaa
     1561 aatctggaaa gtcttagttc ctcaggctcc taggattttt catgctggag actgtggtat
     1621 gcatcacaag aaaacatgta ggccatccac ccagagtgcc caaattgagt cattattaaa
     1681 tagcaacaaa caatacctgt ttccggaaac tctagttatc ggtgagaagt ttcctatggc
     1741 agccatttcc ccacctagga aaaatggagg gtggggagat attagggacc atgaactctg
     1801 taaaagttat agaagactgc agtgaggaaa aaaaatatca caattgcaaa agcaaaagtg
     1861 acagtcttct attttttata tttgtctcaa tgggatatac aattgaataa aggtatagga
     1921 accggcctct gctttaatac ataaacttct tgtaaaaggt gtccagatac atagcaatcc
     1981 tttccagtta ggactgatta aaatttcaaa ctgaaatttt cattttggga ctgggtttta
     2041 aaattcagtc tatctgctta aaagtaatga ttagattatt gataaaaaaa gaaaggggaa
     2101 attttattta aatcacatct gttaatcttt ttatctaaaa ctttgtatac ttttccactt
     2161 tcaaaagtta ttttaagtat atcaagattt atttaaaact gtcaacaaca gtaaaaagta
     2221 ttacaatgaa atcactagag tatttctggg acagacccag tttcactatt gacacagtta
     2281 ttagaaagga atcctttggt tcaataactc agaagtttta agtttgttaa gcatcctgtc
     2341 ggaagagtca ttttcagtat tactgattcc tgtgctattg tgttaagatt tgcctgtgct
     2401 ttcaacatac atattacatg atttcctttg gaatgtattt gattaataag gaagttaaag
     2461 caatgttttc acctcaatgt agaaatacag tggttttgat ttttttcttc ttagtgctgc
     2521 caaaataaaa tactcatttt tgcataaaat tccctaatca ttttgcagaa tcttctattt
     2581 gtatcaataa aggcattcta ggagttttga gaatgaaaaa
//
