LOCUS       NM_001384225            3981 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus major facilitator superfamily domain containing 4B4
            (Mfsd4b4), transcript variant 1, mRNA.
ACCESSION   NM_001384225
VERSION     NM_001384225.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118733.8.
            
            Transcript Variant: Variants 1and 3 encode the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11927938.4563980.1,
                                           ERR3363658.579831.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-293               AC118733.8         54265-54557
            294-778             AC118733.8         57475-57959
            779-947             AC118733.8         58531-58699
            948-1064            AC118733.8         59339-59455
            1065-3981           AC118733.8         60542-63458
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 21.27 cM"
     gene            1..3981
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="major facilitator superfamily domain containing
                     4B4"
                     /db_xref="GeneID:213332"
                     /db_xref="MGI:MGI:3035041"
     exon            1..293
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /inference="alignment:Splign:2.1.0"
     exon            294..778
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /inference="alignment:Splign:2.1.0"
     CDS             761..2272
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="major facilitator superfamily domain containing
                     4B4 isoform 1"
                     /protein_id="NP_001371154.1"
                     /db_xref="GeneID:213332"
                     /db_xref="MGI:MGI:3035041"
                     /translation="MDFNAEGLSVAILGPTFPDLAKNVNRNISSLSLIFVGRASGYLC
                     GSMIGGVLFGCINHFLLLGVSLSATTVGLYLMPFCKTAALLIVMMSVFGVSMGILDTG
                     GNVLILDLWGDKGAPHMQALHFSFALGAFLAPLLAKLAWGTTTSAQNYTEPQSDRLAL
                     NQSFEATSDSVFAVPDDMNLLWTYASIGTYVLVVSVFLFGLFCKKHSRQKKPRASAEG
                     ARRAKYHRALLCLLFLFFFFYVGAEVTYGSFVFSFAITHVGMEESEAAGLNSIFWGTF
                     AACRGLAIFFATFLQPGTMIVLCNIGSLVSCFFLVLFDKSPLCLWIATSVYGASMAAT
                     FPSGISWIEQYTTLTGKSAAFFVIGAALGEMAIPAVIGILQGQYPDLPVVLYTCLGSA
                     IFTAVLFPVMYKLATLPLECQHKGTRKREDQKALLSSSELHDCEEENEEDDAEKWNEM
                     DFEVVEMSDPVQESARETCREEGSGQVLLDGLIAEPFSANSSNSLGNHQDKRD"
     exon            779..947
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1064
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..3981
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2903..2908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="hexamer: AATAAA"
     polyA_site      2941
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="major polyA site"
     polyA_site      3113
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
     regulatory      3951..3956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="hexamer: AATAAA"
     polyA_site      3981
                     /gene="Mfsd4b4"
                     /gene_synonym="213332"
                     /note="major polyA site"
ORIGIN      
        1 ccgctcccag gtcacgctcg cgcgggcagg tcctggcgtt gttggctccc gggccgggcg
       61 gggccgggca cctccctgca caccggaggc ctggtgcacg gagcacggca ggtggagccg
      121 ccacactgga gttggagctg gagttcggag gggcgggagg gcagcggctc ctcccagccg
      181 gcccgggcgc agagaatgag tcagaagcgg tggcctccgt gcggaaaggc cgtgcccaaa
      241 gcgtgctgcg ctggttcacc accgtggtgc tttgtgccgc cttcctgggg ctggagtcca
      301 cagctgagag actggatttt aaagtgtttg aatttgtaaa gattggaacc tttaaagttt
      361 gcaaaatgtt ttatatcgtg ttgttgatgt taaggtggga tcctggggat gaacaagaca
      421 ggaaaggtgg tggcttaaca gtgacgtgtt tgtgtatcag gttgacaggg gtcacttgcc
      481 ctggactagt tttatgtcaa cttgagagat gctaaagtca tctgagagga aggaacctca
      541 gctgagaaaa tctctccaca agatctggct gttaggcatt tcttagtgat tgatggggga
      601 tggcccagcc cattgtgggt gacgctatcc ctggctggtg gtcctgggtt ctataagaag
      661 gcagactgaa caagctgtga ggagcaagcc aggaagcagc accctcccat ggtcccagca
      721 tcagctcctg cctcctgtcc tagcctgcct tccttcaagc atggacttta atgcggaggg
      781 actaagtgtt gctatcctgg ggcccacctt tccagacttg gcgaagaacg tgaacagaaa
      841 catcagcagc ctgtctctga ttttcgtggg ccgggcctca ggatatttat gtggctctat
      901 gattggagga gtcctgtttg gctgtataaa tcatttttta cttttggggg tttccctttc
      961 tgctaccaca gttggtcttt atctcatgcc attctgcaag acagcagccc tactgattgt
     1021 catgatgtct gtcttcgggg tttccatggg cattctggat acaggtggaa acgtcctcat
     1081 cttggatctc tggggggaca aaggagcccc acatatgcag gccttgcact tcagtttcgc
     1141 cttgggtgcc tttctggctc ccctgctggc taaattggcc tggggtacaa caacatctgc
     1201 tcagaattac accgagcccc aatctgaccg tttagccttg aaccaatcct ttgaagccac
     1261 ctcagactct gtgttcgcag tacccgatga catgaatctg ctgtggacat atgcttctat
     1321 tggcacctat gttttagtag tttctgtctt tctgtttggt ctgttttgta agaaacattc
     1381 aaggcagaaa aaacccagag catctgctga gggagctcga agggctaaat atcacagggc
     1441 cctcctatgc ctgctcttcc tcttcttctt cttctatgtg ggagccgagg tgacctacgg
     1501 ctctttcgta ttctcctttg ctatcaccca tgttggcatg gaagagagcg aggcagctgg
     1561 cttgaactcc atcttctggg gaacctttgc agcctgcagg ggcctggcca tcttctttgc
     1621 aacattctta cagcctggaa ccatgattgt gttgtgtaac attggcagcc tggtgtcatg
     1681 tttctttctg gtgctttttg acaagagccc tctttgtctc tggatagcga cttctgtgta
     1741 tggagcctca atggcagcca cgtttcccag cggcatctcc tggattgagc aatacaccac
     1801 cttaactggg aaatctgcag cattctttgt aattggcgcg gccctgggag aaatggccat
     1861 tcctgcagtg atcggaattc ttcagggaca gtacccagat ctgccagtag ttctgtacac
     1921 atgtctgggc tcagccatat tcacagctgt tttatttcct gtgatgtata aattagccac
     1981 cttgcccctg gaatgccagc acaaaggaac aaggaagaga gaggaccaga aagctctact
     2041 ctccagctct gagctacacg actgtgagga agagaatgag gaggacgatg cagagaagtg
     2101 gaacgagatg gattttgagg tggttgaaat gagtgatccc gttcaggagt ctgcaaggga
     2161 gacttgtaga gaagagggct ctggtcaggt ccttctggat ggactgattg ctgagccctt
     2221 ttcagccaac agcagtaatt ctcttgggaa tcatcaggac aaaagggatt aacacgaatt
     2281 ggcttactgc gtgactgcca ctcctgagga caccattcag ggcagagctc tggctgactt
     2341 tgagcacgct cttgcacttg aaatttgtgt ctgtttacag gaagcagtga gatcatttgg
     2401 agtgtttcga attctcagtc ttaccgttcg ttgtgcactg ggatcttgtt aggagactgt
     2461 tttgtagtct ccaccttcca gaaggtgttg gtgtgtgaag agctgggtag tgtgccaggg
     2521 gtcagcactg tagaaaatga gctgatttgc tgagcaaaga cctgtgctgt gcagtcacac
     2581 cccgacacga gccatttgtc atgacaaaga gcatctttgg agatgggaag atagttttct
     2641 tttaccctct gtgtagggtt ggactgaaaa atactgctga ctatttattc ccacacatag
     2701 gctatgcttc ctccctgact tcatgctcag ctctgacaaa aacctcctgt gattctagaa
     2761 atgcaatgag attattttta acctccaggg attgtgtttg ttttgtttct tgagtcttta
     2821 tagtccaggc tggcctggaa tttactaagt aggccaggtt ggcctctaac tcactgtttt
     2881 gtttctgtct cctgagtttt gtaataaaga cgtgtaccac catgcatggt gaaacatgta
     2941 agttttaaag ttgtatcata tttttgctaa aataatccat tcatttcaaa tgtacaaacc
     3001 aaaactgccg ggtagcccta tagtgctgga gcttgtgcaa tcctgacgtt agacaggcat
     3061 ggtatttagt ggcactgctg cgttcattga tctacaacat gacctttccc ttactctcaa
     3121 gtgtcatcat ttagtattct agtaaatttt aattcttgtt tccctcacat ttaagaaaaa
     3181 ccaaaaccat cttgccaatc actcttgtgt tcattggtac agcggttcat tgcatttcag
     3241 agattatagt atttttgctg acactttaaa aagaggttat ttttatatgc tgttgtctgt
     3301 gtcattcaga gagcatctgg ggacacgttg attttatatt atcttcttag cttttacttt
     3361 tgtctagtgc tgttgacttg acacggggcc ttgcacttgc tagttaagtg agctaccagt
     3421 gagctacgtg tccagctctt aggttttact tataggagct gttttaaagt tactatgtaa
     3481 gttgcagcgg tatgaacggg tcctctggtg ccctcagcct gcctcattag caaaggctgg
     3541 agctgggtct tgccgtttta ggatttgacc ctcactgggt tggggtatta catatattct
     3601 gtaagccagg ctaaaagttt agtttacaga taaggcctca tctggttgtg gtgattggag
     3661 agcccatgtc cacctccagg accactccat gagtttgaag tacctggaaa ccaaatcaag
     3721 atggaagaca gtgggatggt caaggatgag gagtacagct tcacaagtgt gacagagcta
     3781 gcctgggcca gcctttggtg actctagtct gggccaacct tttgtaaatg actttctcaa
     3841 gttttatgct gagtgacaat gaattgctat gtgactgcaa tatgtttgaa atctcttata
     3901 aaattttatg aatttgtttt tttaaagtgc tttcctaaaa aaaacattat aataaagatt
     3961 aatatttttt tctgtgtgtg a
//
