LOCUS       NM_144821               2679 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus major facilitator superfamily domain containing 4B1
            (Mfsd4b1), mRNA.
ACCESSION   NM_144821
VERSION     NM_144821.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Thiagarajan,R.D., Georgas,K.M., Rumballe,B.A., Lesieur,E.,
            Chiu,H.S., Taylor,D., Tang,D.T., Grimmond,S.M. and Little,M.H.
  TITLE     Identification of anchor genes during kidney development defines
            ontological relationships, molecular subcompartments and regulatory
            pathways
  JOURNAL   PLoS One 6 (2), e17286 (2011)
   PUBMED   21386911
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC168279.3.
            
            On May 1, 2010 this sequence version replaced NM_144821.3.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus. The use of an alternative upstream start codon changes
            the protein length from 377 aa to 425 aa, but does not affect
            splicing. The uAUG appears to be mouse-specific (CTG in other
            species), but the extension does result in a predicted N-terminal
            signal peptide (aa 1-20 per SignalP 3.0; not with high probability)
            and adds another transmembrane domain to the protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK089961.1, BC018406.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-376               AC168279.3         162317-162692       c
            377-545             AC168279.3         144684-144852       c
            546-662             AC168279.3         143402-143518       c
            663-2679            AC168279.3         138997-141013       c
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 21.43 cM"
     gene            1..2679
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /note="major facilitator superfamily domain containing
                     4B1"
                     /db_xref="GeneID:215929"
                     /db_xref="MGI:MGI:2143575"
     exon            1..376
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /inference="alignment:Splign:2.1.0"
     CDS             353..1630
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /note="major facilitator superfamily domain-containing
                     protein 4B1"
                     /codon_start=1
                     /product="sodium-dependent glucose transporter 1A"
                     /protein_id="NP_659070.2"
                     /db_xref="CCDS:CCDS35884.2"
                     /db_xref="GeneID:215929"
                     /db_xref="MGI:MGI:2143575"
                     /translation="MYAAFLGLGMSVAILGPTFQDLAENVNRNISSLSLIFVGRATGF
                     LSGTMIGGVLFDHINQFFLLGASMVATAAGLYLIPFCKTAVLLIITMSVFGASVGVVD
                     TGANVLILDLWGDKGAPQMQALHFSFALGAFLAPLLAKLAWGTAPAQNHTESDLDTLM
                     LNRSSNGTSDSVFAVPDDMNLLWAYASIGTFILVVSVFLFGLFCKKHSRQKKPRASAE
                     GARRAKYHRALLCLLFLFFFFYVGAEITYGSYIFSFATTHVGMEESEAAGLNSIFWGT
                     FAACRGLAIFFATFLQPGTMIVLSNIGSLVSCFFLVLFDKSPLCLWIATSVYGASMAA
                     TFPSGISWIEQYTTLTGKSAAFFVIGSALGDMAIPAVIGILQGHYPDLPVVLYTCLGS
                     AIFTAILFPVMYKLATLPLKREDQKALPTSSRL"
     exon            377..545
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /inference="alignment:Splign:2.1.0"
     exon            546..662
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /inference="alignment:Splign:2.1.0"
     exon            663..2679
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2649..2654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /note="hexamer: AATAAA"
     polyA_site      2673
                     /gene="Mfsd4b1"
                     /gene_synonym="Naglt1a"
                     /note="major polyA site"
ORIGIN      
        1 acgattctgc aagatggaga gtgaacataa ctcggtggcc agctggggcc agggtcgaag
       61 tccagccgcc ggaggtcttg agtcaggacg gtccctgggg acagtgctat caggagggac
      121 ctcggagact ggaggagcct gagtgccgag ccccagaggg tgcaccggcc cccagtagct
      181 ctccgcagcc gccaagctgg agctggacgt gaagaccggg agcgtcgggc cggtggctgc
      241 ggcacagagg ctccaccagg cggagacccg ggcggagagc gagcaggcca cggtggcttc
      301 ccggcggagt ggccggacca ggagcgtgct gcgctggttc tccaccgcgg tcatgtatgc
      361 cgccttcctg gggctgggaa tgagtgttgc tatcctggga cccacatttc aagacctggc
      421 agagaatgtc aacagaaaca tcagcagcct gtctctgatt ttcgtgggcc gtgccactgg
      481 gttcttgtct ggcaccatga ttggcggggt tctctttgac cacataaatc aattctttct
      541 tttgggagcc tcaatggtgg caactgcagc gggactttat ctcatcccgt tctgcaagac
      601 agcagtctta ttgatcatca ccatgtctgt ctttggagct tccgttggtg ttgtggatac
      661 aggtgcaaat gttctcatct tggatctctg gggggacaaa ggagccccac aaatgcaggc
      721 cttgcacttc agtttcgcct tgggtgcctt tctggctccc ctgctggcta agttggcctg
      781 gggtacagca cctgctcaga accacaccga gtccgacctt gacactctga tgctgaaccg
      841 atcctccaac ggcacctcag actccgtgtt tgcggtaccc gatgacatga atctgctgtg
      901 ggcatatgct tccattggca cctttatttt agtagtttct gtctttctgt ttggtctgtt
      961 ttgtaagaaa cattcaaggc agaaaaaacc cagagcatct gctgagggag ctcgaagggc
     1021 taaatatcac agggccctgc tatgcctgct cttcctcttc ttcttcttct atgtgggagc
     1081 cgagataaca tacggctctt acatattctc cttcgccacc acccatgttg gcatggaaga
     1141 gagcgaggca gctggcttga attccatctt ctggggaacc tttgcagcct gcaggggcct
     1201 ggccatcttc tttgcgacat tcttacagcc tggaaccatg atcgtgctga gcaacattgg
     1261 cagcctggtc tcatgtttct ttctggtact ttttgacaag agccctcttt gtctctggat
     1321 cgcgacttct gtgtatggag cctcaatggc agccacgttt cccagcggca tctcctggat
     1381 tgagcagtac accaccttaa ctgggaaatc tgcagcattc tttgtaattg gctctgccct
     1441 gggagatatg gccattccag cggtgatcgg aattcttcag ggacactacc cagatctgcc
     1501 agtagttctg tacacatgtc tgggctcagc catattcaca gctattttat ttcctgtgat
     1561 gtataaatta gctaccttgc ccctgaagag agaggaccag aaagctttgc ccactagttc
     1621 tagactgtga ggaagagact acatgagaac ttaaaaaaaa aaaaaaggaa tgaaacagat
     1681 tttgaagttg cagtgagtgg tccttgaggc catgtgtaat ggagacgtct ggagatgttg
     1741 tgatggagcc cacagagtgc ctttctattg agttctctac actgaataaa agcaaccccc
     1801 ctgagacaca cctggacaaa agggacatat actgagagag aatgaattac ttagcagctt
     1861 taccaaataa agagttcaat caactggaaa gtgaagattt ttccaaaacc tattggtgct
     1921 caagctttct gacctatttg tagagaatgc tagaaaattt agaaatgttc tatgattctc
     1981 agtcatccat aaactaccaa atggtctcat atagtaagat ctcactagga gacttctttt
     2041 tttggcagac gaataattag gtagtgttta tggtcaccac ctttcagagc ctaggaagaa
     2101 ggcagaggag gttgggaagc tgtgggcatt ctgatatatc agctgggttt tctcagcagt
     2161 gaccacccac ccacattcta ccatgtgctt gtgaaatctt tcttgtaaga gtcagatgtt
     2221 gctataactg ctgtagacct gggtcacagc atgccagagt gacaaagacc gatttcagat
     2281 ttttactgct acaaaggaaa cacagagcag gatttaagtt atgcatagct cttcaacatg
     2341 gctctgcttt caggtcctcc tgggggctct gagtcatatc ctctaggtca tatccctgaa
     2401 gataagatga ttcctaaatt gataatctat gcatcataaa gtttttgcaa gatgtctcct
     2461 agaaactaat gtaacctcta gcctactctt cattgcaaat gttaaatgaa ccaaagatga
     2521 taaaaagatt aattaacaat ttgatactga ttacatgtgc catttaatgt acttaatgct
     2581 taatcttcct ttgaaaaata ctatttcagt gcagaattgg gcacatttga aaccaggata
     2641 ttccttaaaa taaacaatca tttaatattg ggacaaagc
//
