LOCUS       NM_001033488            3125 bp    mRNA    linear   ROD 03-FEB-2025
DEFINITION  Mus musculus MFSD2 lysolipid transporter B, sphingolipid (Mfsd2b),
            mRNA.
ACCESSION   NM_001033488 XM_484111 XM_902250 XM_902253 XM_902255 XM_902258
            XM_902261 XM_902263 XM_905976 XM_919594 XM_919603 XM_919612
            XM_919619 XM_919624 XM_919636 XM_919641
VERSION     NM_001033488.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Duse,D.A., Schroder,N.H., Srivastava,T., Benkhoff,M., Vogt,J.,
            Nowak,M.K., Funk,F., Semleit,N., Wollnitzke,P., Erkens,R.,
            Kotter,S., Meuth,S.G., Keul,P., Santos,W., Polzin,A., Kelm,M.,
            Kruger,M., Schmitt,J. and Levkau,B.
  TITLE     Deficiency of the sphingosine-1-phosphate (S1P) transporter Mfsd2b
            protects the heart against hypertension-induced cardiac remodeling
            by suppressing the L-type-Ca2+ channel
  JOURNAL   Basic Res Cardiol 119 (5), 853-868 (2024)
   PUBMED   39110173
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3125)
  AUTHORS   Thomas,N., Schroder,N.H., Nowak,M.K., Wollnitzke,P., Ghaderi,S.,
            von Wnuck Lipinski,K., Wille,A., Deister-Jonas,J., Vogt,J.,
            Graler,M.H., Dannenberg,L., Buschmann,T., Westhoff,P., Polzin,A.,
            Kelm,M., Keul,P., Weske,S. and Levkau,B.
  TITLE     Sphingosine-1-phosphate suppresses GLUT activity through PP2A and
            counteracts hyperglycemia in diabetic red blood cells
  JOURNAL   Nat Commun 14 (1), 8329 (2023)
   PUBMED   38097610
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3125)
  AUTHORS   Polzin,A., Dannenberg,L., Benkhoff,M., Barcik,M., Helten,C.,
            Mourikis,P., Ahlbrecht,S., Wildeis,L., Ziese,J., Zikeli,D.,
            Metzen,D., Hu,H., Baensch,L., Schroder,N.H., Keul,P., Weske,S.,
            Wollnitzke,P., Duse,D., Saffak,S., Cramer,M., Bonner,F., Muller,T.,
            Graler,M.H., Zeus,T., Kelm,M. and Levkau,B.
  TITLE     Revealing concealed cardioprotection by platelet Mfsd2b-released
            S1P in human and murine myocardial infarction
  JOURNAL   Nat Commun 14 (1), 2404 (2023)
   PUBMED   37100836
  REMARK    GeneRIF: Revealing concealed cardioprotection by platelet
            Mfsd2b-released S1P in human and murine myocardial infarction.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3125)
  AUTHORS   Le,T.N.U., Nguyen,T.Q., Kalailingam,P., Nguyen,Y.T.K.,
            Sukumar,V.K., Tan,C.K.H., Tukijan,F., Couty,L., Hasan,Z., Del
            Gaudio,I., Wenk,M.R., Cazenave-Gassiot,A., Camerer,E. and
            Nguyen,L.N.
  TITLE     Mfsd2b and Spns2 are essential for maintenance of blood vessels
            during development and in anaphylactic shock
  JOURNAL   Cell Rep 40 (7), 111208 (2022)
   PUBMED   35977478
  REMARK    GeneRIF: Mfsd2b and Spns2 are essential for maintenance of blood
            vessels during development and in anaphylactic shock.
REFERENCE   6  (bases 1 to 3125)
  AUTHORS   Nguyen,T.Q., Vu,T.M., Tukijan,F., Muralidharan,S., Foo,J.C., Li
            Chin,J.F., Hasan,Z., Torta,F. and Nguyen,L.N.
  TITLE     Erythrocytes efficiently utilize exogenous sphingosines for S1P
            synthesis and export via Mfsd2b
  JOURNAL   J Biol Chem 296, 100201 (2021)
   PUBMED   33334894
  REMARK    GeneRIF: Erythrocytes efficiently utilize exogenous sphingosines
            for S1P synthesis and export via Mfsd2b.
REFERENCE   7  (bases 1 to 3125)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3125)
  AUTHORS   Kobayashi,N., Kawasaki-Nishi,S., Otsuka,M., Hisano,Y., Yamaguchi,A.
            and Nishi,T.
  TITLE     MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells
  JOURNAL   Sci Rep 8 (1), 4969 (2018)
   PUBMED   29563527
  REMARK    GeneRIF: Using microarray analysis of erythroid cell lines
            overexpressing human and mouse MFSD2B study showed sphingosine
            1-phosphate (S1P) transport activity. The properties of S1P release
            by MFSD2B were similar to those in erythrocytes. Moreover, knockout
            of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells.
            These results strongly suggest that MFSD2B is a novel S1P
            transporter in erythroid cells.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3125)
  AUTHORS   Vu,T.M., Ishizu,A.N., Foo,J.C., Toh,X.R., Zhang,F., Whee,D.M.,
            Torta,F., Cazenave-Gassiot,A., Matsumura,T., Kim,S., Toh,S.E.S.,
            Suda,T., Silver,D.L., Wenk,M.R. and Nguyen,L.N.
  TITLE     Mfsd2b is essential for the sphingosine-1-phosphate export in
            erythrocytes and platelets
  JOURNAL   Nature 550 (7677), 524-528 (2017)
   PUBMED   29045386
REFERENCE   10 (bases 1 to 3125)
  AUTHORS   Angers,M., Uldry,M., Kong,D., Gimble,J.M. and Jetten,A.M.
  TITLE     Mfsd2a encodes a novel major facilitator superfamily
            domain-containing protein highly induced in brown adipose tissue
            during fasting and adaptive thermogenesis
  JOURNAL   Biochem J 416 (3), 347-355 (2008)
   PUBMED   18694395
  REMARK    GeneRIF: Results identify Mfsd2a (major facilitator superfamily
            domain-containing protein 2a) and an additional closely related
            protein Mfsd2b, and suggest that Mfsd2a plays a role in adaptive
            thermogenesis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT571254.7.
            
            On Nov 15, 2007 this sequence version replaced NM_001033488.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172423.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-89                CT571254.7         34064-34152         c
            90-215              CT571254.7         31169-31294         c
            216-340             CT571254.7         30292-30416         c
            341-464             CT571254.7         29328-29451         c
            465-543             CT571254.7         28925-29003         c
            544-674             CT571254.7         28706-28836         c
            675-765             CT571254.7         27405-27495         c
            766-887             CT571254.7         27131-27252         c
            888-971             CT571254.7         26583-26666         c
            972-1052            CT571254.7         26242-26322         c
            1053-1162           CT571254.7         25952-26061         c
            1163-1306           CT571254.7         25521-25664         c
            1307-1483           CT571254.7         24930-25106         c
            1484-3125           CT571254.7         22231-23872         c
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 2.11 cM"
     gene            1..3125
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /note="MFSD2 lysolipid transporter B, sphingolipid"
                     /db_xref="GeneID:432628"
                     /db_xref="MGI:MGI:3583946"
     exon            1..89
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1508
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /note="major facilitator superfamily domain-containing
                     protein 2b; mMfsd2b; major facilitator superfamily domain
                     containing 2B"
                     /codon_start=1
                     /product="sphingosine-1-phosphate transporter MFSD2B"
                     /protein_id="NP_001028660.2"
                     /db_xref="CCDS:CCDS36401.2"
                     /db_xref="GeneID:432628"
                     /db_xref="MGI:MGI:3583946"
                     /translation="MSVPHGPTPAPVAEPHTQEPGSDKRDGRLSVCTKVCYGIGGVPN
                     QVASSASAFYLQLFLLDVAQIPAAQVSLALFGGKVSGAVADPVAGFFINKSRRTGSGR
                     LMPWALGCMPLIALAYFFLWFLPPFTSLRGLWYTSFYCLFQALATFFQVPYTALTMIL
                     TPSPRERDSATAYRMTMEMAGTLMGATVHGLIVSSAHGSQRCEDTVHPRSPAVSPDVA
                     RLYCIAAAVVALTYPVCGSLLCLGVKEQPDTSAPASGQGLNFFTGLAITSQHPPYLSL
                     VVSFLFISAAVQVEQSYLVLFCTHASKLQDHVQNLVLIILVSAVLSTPLWEWVLQRFG
                     KKTSAFGICVMVPFSILLAAVPSAPVAYVVAFVSGVSIAVSLLLPWSMLPDVVDDFQL
                     QHRCGPGVETIFYSSYVFFTKLSGAGALGISTLSLEFAGYEAGACQQAEEVVVTLKVL
                     IGAVPTCMILIGLCILLVGPTPKMPRQDTSSQLSLRRRTSYSLA"
     exon            90..215
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            216..340
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            341..464
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            465..543
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            544..674
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            675..765
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            766..887
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            888..971
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1052
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1162
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1306
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1483
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     exon            1484..3125
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1980..1985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /note="hexamer: TATAAA"
     polyA_site      2000
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /note="major polyA site"
     regulatory      2494..2499
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
                     /note="hexamer: AATAAA"
     polyA_site      2535
                     /gene="Mfsd2b"
                     /gene_synonym="Gm1964"
ORIGIN      
        1 agtcggagct taacagccac gggatgtcgg tgccccatgg acccaccccc gctcctgtcg
       61 cagagcctca cacccaggag ccaggctcgg acaagagaga tggtcgcctc tcagtctgta
      121 caaaggtgtg ctatggcatt ggtggggtcc ccaaccaggt ggcctccagc gcctcagcct
      181 tctacctgca actcttccta ctggatgtgg cccagatccc cgctgcccag gtgtcacttg
      241 ccttgtttgg agggaaggtg tctggggcag ttgcagaccc tgtggctggc ttcttcatca
      301 acaagagcag aaggacgggg tctggacggc taatgccttg ggcgctgggc tgcatgccct
      361 taatcgcact ggcctacttc ttcctgtggt tcctgccccc attcaccagc cttcgtgggc
      421 tctggtatac cagcttctac tgcctgttcc aggccctggc tacgttcttc caggtgccct
      481 acactgcgct cactatgatc ctcaccccca gccccaggga gcgagactct gccaccgcat
      541 accggatgac catggagatg gcagggacac tgatgggagc cactgtccac gggctcattg
      601 tgtccagtgc ccacgggtcc cagaggtgtg aggacactgt gcaccccagg agtcctgctg
      661 tttccccaga tgtagctcgc ctctattgta ttgcagctgc cgtggttgcc ttgacttatc
      721 ctgtgtgtgg aagtctgctg tgcctaggag tgaaggagca gccagatact tctgccccag
      781 cctcaggcca aggcctgaac ttcttcactg gcctggccat cacttcccag cacccaccct
      841 acctgagcct ggtggtctct ttcttgttca tttcagctgc cgtccaggtg gaacaaagtt
      901 acctagtcct gttctgtaca cacgcctcta agctccaaga tcatgttcag aacttggtgc
      961 tcatcattct ggtctcagct gtgctgagca ccccactgtg ggagtgggtt ctccagcgat
     1021 ttgggaagaa gacatctgca ttcggcatct gtgtgatggt gcccttttct atcttgttgg
     1081 ctgctgtgcc ctcagcaccg gtggcctatg ttgtggcctt tgtctctggc gtgagcattg
     1141 ctgtgtccct gctgctaccc tggtccatgc tgccagatgt ggtggatgac tttcagctgc
     1201 agcatcggtg tggcccaggc gtggagacca tcttctactc atcctacgtc ttcttcacca
     1261 agctgtcagg cgcgggtgcc ttgggcatct ctaccctcag tttggagttt gcagggtatg
     1321 aggcaggggc ctgccagcaa gcagaagaag tggtggtcac tctcaaggtc ctcatcggtg
     1381 ctgtgcccac ctgtatgatc cttattggcc tgtgcatcct cctggttggc cccactccca
     1441 agatgccacg tcaggacact tcctcccagc taagccttcg gaggaggact agctacagcc
     1501 ttgcctgaac tttaaggaga cctctgtgag ctggaaagga gaaacctgca ttttctggaa
     1561 ctctctagcc cacagctcct tgcctgttgg acctggatcc tttcaggctt gtaccccaga
     1621 ctgctccacc ttcaagaaac ctgattctaa aagcatcctc ctggaaggtt tccagcatca
     1681 ggaagccctg cctaccctgg ccctgtacat tccttttgct aaatggcaga gttccaacac
     1741 aggaattcag acacaggcct ggcttgcatt cacactccag tggccacccg gacacagtgc
     1801 aaatggtcag gctgaggaaa caggacatgt tggaagctaa gcaggtattt atccagcatt
     1861 ttgctctgtt cacaccagtt gaggttactt ctatccatag acagaggatg acatggaacc
     1921 atcagaggcc agctactttt acaaacaaat tgtgaaatat tctgaaattc tatggtatat
     1981 ataaaaccat atactaggaa ttacagtttt ctagggtctc tgtctttggg gaagaaagat
     2041 tcactggggt ttatattttt ttaaaaaaac aagtttagct gggtatggtg acgcacacat
     2101 gcagtcccag cattggggaa cagaagcagg aaaatcatga gttagaggcc agcctccact
     2161 acatatcaag tttgaggcca gcctgaagaa cctgagaccc tgtcttcaaa gcaaataaaa
     2221 actaaagctt atttcctacc acgtgggtaa aatgtgacca tgggtgccct accatcccat
     2281 gctagagtcc ctggatggga agccattaag catagttctg gccccagcca ccgatcaggc
     2341 accgtattgt gtggatgaag taggaaagat cagggccacc cccagcccaa aagcaaacag
     2401 cttgtccctt attgctctcc tcacctcttg ccactcctat cgcctccttc cctgccaccc
     2461 tggaagccca ttaaacttgg tttcagcctc caaaataaag acagctctgc tccacaggac
     2521 agactccagc acacacaaga gagaaggaag tttgtaccct gtttccactt ttgccacaag
     2581 taactgcttg atttacttgt tttttctaaa gatttatttc ctttatgtgt atatgtttgt
     2641 gcctgagtgt acatgcatgt agatgccggc agagaccagc agagggcatc agagcccctg
     2701 gagctatagt tgtgaggggt tgtaggctgc ccagtaccgg tgctggttac caaacccagg
     2761 ccctctgcaa gagcagcaaa tgctgcgaac agtgttactt tgttgtagtt tgagacaagg
     2821 tctcatgtag ccgaggttgg ctttaaactg atcctgctgc ctttagcact caagtaccag
     2881 gattacaagc atggatcaca tctggtaatc ttgctttaaa aaaatctaag tttagttgtc
     2941 tagacaagag gtatgttgcg ggctggagag atggctcagt gagtaagagc acttccgaag
     3001 gtcctgagtt caaatcccag caaccacatg gtggctcaca ccacccgtaa tgagatctga
     3061 tgccctcttc tggtgtgcct gaagacagct agagtgtact tatgtatatt aataaataaa
     3121 tcttt
//
