LOCUS       NM_008246               2720 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus major facilitator superfamily domain containing 14A
            (Mfsd14a), transcript variant 1, mRNA.
ACCESSION   NM_008246
VERSION     NM_008246.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Doran,J., Walters,C., Kyle,V., Wooding,P., Hammett-Burke,R. and
            Colledge,W.H.
  TITLE     Mfsd14a (Hiat1) gene disruption causes globozoospermia and
            infertility in male mice
  JOURNAL   Reproduction 152 (1), 91-99 (2016)
   PUBMED   27107036
  REMARK    GeneRIF: Loss of Mfsd14a expression is associated with
            globozoospermia and male infertility.
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   3  (bases 1 to 2720)
  AUTHORS   Matsuo,N., Kawamoto,S., Matsubara,K. and Okubo,K.
  TITLE     Cloning of a cDNA encoding a novel sugar transporter expressed in
            the neonatal mouse hippocampus
  JOURNAL   Biochem Biophys Res Commun 238 (1), 126-129 (1997)
   PUBMED   9299464
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131038.6.
            
            On Jul 26, 2022 this sequence version replaced NM_008246.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169626.1, AK004779.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-221               AC131038.6         115245-115465       c
            222-317             AC131038.6         104254-104349       c
            318-380             AC131038.6         101369-101431       c
            381-508             AC131038.6         100632-100759       c
            509-623             AC131038.6         98216-98330         c
            624-842             AC131038.6         94560-94778         c
            843-956             AC131038.6         94097-94210         c
            957-1028            AC131038.6         93237-93308         c
            1029-1141           AC131038.6         89128-89240         c
            1142-1239           AC131038.6         87753-87850         c
            1240-1403           AC131038.6         86572-86735         c
            1404-2720           AC131038.6         84014-85330         c
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 50.42 cM"
     gene            1..2720
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="major facilitator superfamily domain containing
                     14A"
                     /db_xref="GeneID:15247"
                     /db_xref="MGI:MGI:1201609"
     exon            1..221
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..1610
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     tetracycline transporter-like protein; hippocampus
                     abundant transcript 1 protein; hippocampus abundant gene
                     transcript 1"
                     /codon_start=1
                     /product="hippocampus abundant transcript 1 protein
                     isoform 1"
                     /protein_id="NP_032272.2"
                     /db_xref="CCDS:CCDS17790.1"
                     /db_xref="GeneID:15247"
                     /db_xref="MGI:MGI:1201609"
                     /translation="MTQGKKKKRAANRSIMLAKKIIIKDGGTPQGIGSPSVYHAVIVI
                     FLEFFAWGLLTAPTLVVLHETFPKHTFLMNGLIQGVKGLLSFLSAPLIGALSDVWGRK
                     SFLLLTVFFTCAPIPLMKISPWWYFAVISVSGVFAVTFSVVFAYVADITQEHERSMAY
                     GLVSATFAASLVTSPAIGAYLGRVYGDSLVVVLATAIALLDICFILVAVPESLPEKMR
                     PASWGAPISWEQADPFASLKKVGQDSIVLLICITVFLSYLPEAGQYSSFFLYLRQIMK
                     FSPESVAAFIAVLGILSIIAQTIVLSLLMRSIGNKNTILLGLGFQILQLAWYGFGSEP
                     WMMWAAGAVAAMSSITFPAVSALVSRTADADQQGVVQGMITGIRGLCNGLGPALYGFI
                     FYIFHVELKELPITGTDLGTNTSPQHHFEQNSIIPGPPFLFGACSVLLALLVALFIPE
                     HTNLSLRSSSWRKHCGSHSHPHSTQAPGEAKEPLLQDTNV"
     exon            222..317
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            318..380
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            381..508
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            509..623
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            624..842
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            843..956
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1028
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1141
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1239
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1403
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..2720
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1863..1868
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="hexamer: ATTAAA"
     polyA_site      1881
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="major polyA site"
     regulatory      2563..2568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="hexamer: CATAAA"
     polyA_site      2583
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
     regulatory      2680..2685
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="hexamer: AATAAA"
     regulatory      2686..2691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="hexamer: ATTAAA"
     polyA_site      2702
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
     polyA_site      2720
                     /gene="Mfsd14a"
                     /gene_synonym="Hiat1"
                     /note="major polyA site"
ORIGIN      
        1 gaggcaggcc gggccctcaa gatggcggcg tgcgccctga gcggctcggc ccggcagtag
       61 tggcgggacc gcactcgccg ctggggccgc ccgccccctg agtggctgct gcagcgccgg
      121 gagtcgagga tggtaaaatg acccagggga agaaaaagaa acgggccgcg aaccgcagta
      181 tcatgctggc caaaaagatc atcattaagg acggaggcac gcctcaagga ataggctccc
      241 ctagtgtgta tcatgcggtt attgtcatct ttttggagtt ttttgcttgg ggactactga
      301 cagcacccac attggtggta ttgcatgaaa ccttccctaa acatacattt ctgatgaatg
      361 gcctaattca aggagtaaag ggtttgttgt cattcctcag tgccccactt attggtgctc
      421 tttctgatgt ttggggccga aagtccttcc tgctgctaac agtgtttttc acgtgtgccc
      481 ctattcctct gatgaagatc agcccgtggt ggtactttgc tgttatctct gtttctgggg
      541 tttttgcagt aaccttctct gtggtatttg catatgtagc agatataact caagagcatg
      601 aaaggagtat ggcttacgga ctggtttctg ctacgttcgc tgccagctta gttaccagcc
      661 ctgcaattgg agcttacctt ggacgagtgt atggggacag cttagtggtg gtccttgcta
      721 cagcaatagc tctgctagac atctgtttta tccttgttgc tgtgccagag tcattgcctg
      781 agaagatgcg gccagcatcg tggggagctc ccatttcatg ggaacaggct gacccctttg
      841 catctttaaa aaaagtgggc caagactcca tagtgctgct gatctgtatc acggtgtttc
      901 tctcctacct acctgaagcc ggccagtact ccagcttctt cttatacctc agacagataa
      961 tgaaattttc cccagaaagt gtggcagcct tcatagcagt ccttggcatt ctgtccatta
     1021 ttgcacagac catagtcttg agtttactca tgaggtcaat tggaaataag aacaccatct
     1081 tactgggtct gggatttcaa atattgcagc tggcatggta cggctttggt tccgaacctt
     1141 ggatgatgtg ggctgctggg gcagtggcag ccatgtctag catcaccttt ccagctgtta
     1201 gcgcccttgt ttcacggact gctgacgcgg atcaacaggg tgttgttcaa gggatgataa
     1261 cagggatcag aggattgtgc aatggtctgg gaccagccct ttatgggttc attttctaca
     1321 tattccatgt ggaacttaaa gaactgccaa tcacagggac agacctgggg acaaacacaa
     1381 gccctcagca tcactttgaa cagaattcca tcatccctgg tccccctttc ctctttggag
     1441 cctgttcggt actgctggct ctgctcgttg ccttgtttat tccggaacac actaatttaa
     1501 gtttaaggtc cagcagttgg aggaagcact gtggcagtca cagccatcct cacagtacac
     1561 aagcgccagg agaggccaaa gaacctttac tccaggacac gaatgtgtga cggaaatcag
     1621 gaagacttct ctctctcgca gcacccaggt cttagttttc acctgtagct ctggatgtac
     1681 attccatttc catctgcagt gtactttaag actgtcttaa gaaatacttg catgaactcc
     1741 gtgggaacta aagaaagaac cggacagttt tccaaagatg ttacactttc tttcaaaaag
     1801 aaaccttttg tttatattag caccactctc ttgccactaa actgttttat tacacatctt
     1861 taattaaagc tctatctgta actccgtctc tgccatttcc ttaggtgttg agcctttacc
     1921 tgctcgtgct gactcagcga cactaggcag gatggttatg gacctgtttg ctttaacctt
     1981 gtaaaacctg agtcattgta aaatcttggg tcaaataatt caaagcctta atgcagatgc
     2041 actagaaaag aaatggtaaa gaattgtttg catttaaaag aacttgtgaa aattgtagaa
     2101 aaccagaatc atgtttcaag cttgtttgcc ataattttat ttaaagcatt attcacggct
     2161 gttctgaaga atgaaaagat cagccactta gaattcaaat tggggtggtc agagcctagc
     2221 catctggctc aatctgttta aatctgttct gcttcttata ccactattct tttgatattt
     2281 gcataatcat aaggacctca acacttgaat acataatcta aaaattatat agtaaagctg
     2341 gtagccttga aaatgtcaat gtgatatcta tatgtagata aatatatata gtggcctttc
     2401 aggactgtca cagtaacact ttatttacag agctaatgtt tgtcctaaat tttcaggacc
     2461 ctagaagaga gctttataca attactgatg tgaatttctc taaagtgtat atttttgtgt
     2521 ccagttatat tatttaaaaa agtgttactt tgtaaaaatg tacataaagt attgtatagt
     2581 ttacactgtt ttcatcttgt gtgtggttac tgcctaatgc tttttaaact tggagcattc
     2641 actatgatta aataaggtct caaatgtaaa tattctgtta ataaaattaa atattttaat
     2701 gatttttttc tttttaaaaa
//
