LOCUS       NM_025813               2978 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus major facilitator superfamily domain containing 1
            (Mfsd1), mRNA.
ACCESSION   NM_025813
VERSION     NM_025813.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Boytsov,D., Madej,G.M., Horn,G., Blaha,N., Kocher,T., Sitte,H.H.,
            Siekhaus,D., Ziegler,C., Sandtner,W. and Roblek,M.
  TITLE     Orphan lysosomal solute carrier MFSD1 facilitates highly selective
            dipeptide transport
  JOURNAL   Proc Natl Acad Sci U S A 121 (13), e2319686121 (2024)
   PUBMED   38507452
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2978)
  AUTHORS   Lopez,D.M., Kahlau,L., Jungnickel,K.E.J., Low,C. and Damme,M.
  TITLE     Characterization of the complex of the lysosomal membrane
            transporter MFSD1 and its accessory subunit GLMP
  JOURNAL   FASEB J 34 (11), 14695-14709 (2020)
   PUBMED   32959924
  REMARK    GeneRIF: Characterization of the complex of the lysosomal membrane
            transporter MFSD1 and its accessory subunit GLMP.
REFERENCE   4  (bases 1 to 2978)
  AUTHORS   Massa Lopez,D., Thelen,M., Stahl,F., Thiel,C., Linhorst,A.,
            Sylvester,M., Hermanns-Borgmeyer,I., Lullmann-Rauch,R., Eskild,W.,
            Saftig,P. and Damme,M.
  TITLE     The lysosomal transporter MFSD1 is essential for liver homeostasis
            and critically depends on its accessory subunit GLMP
  JOURNAL   Elife 8, e50025 (2019)
   PUBMED   31661432
  REMARK    GeneRIF: Mfsd1 knockout mice develop splenomegaly and severe liver
            disease. Proteomics of isolated lysosomes from Mfsd1 knockout mice
            revealed GLMP as a critical accessory subunit for MFSD1. MFSD1 and
            GLMP physically interact. GLMP is essential for the maintenance of
            normal levels of MFSD1 in lysosomes and vice versa.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2978)
  AUTHORS   Perland,E., Hellsten,S.V., Lekholm,E., Eriksson,M.M., Arapi,V. and
            Fredriksson,R.
  TITLE     The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC
            Transporters Affected by Altered Nutrient Intake
  JOURNAL   J Mol Neurosci 61 (2), 199-214 (2017)
   PUBMED   27981419
  REMARK    GeneRIF: Protein expression of MFSD1 is in mouse brain regions is
            specifically affected by altered nutrient uptake.
REFERENCE   6  (bases 1 to 2978)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   7  (bases 1 to 2978)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135635.5.
            
            On Apr 29, 2007 this sequence version replaced NM_025813.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK135235.1, AK004586.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-210               AC135635.5         92921-93130
            211-263             AC135635.5         94797-94849
            264-376             AC135635.5         95751-95863
            377-419             AC135635.5         97454-97496
            420-487             AC135635.5         98071-98138
            488-596             AC135635.5         99984-100092
            597-699             AC135635.5         102723-102825
            700-798             AC135635.5         104452-104550
            799-910             AC135635.5         104921-105032
            911-967             AC135635.5         106322-106378
            968-1124            AC135635.5         106747-106903
            1125-1222           AC135635.5         108181-108278
            1223-1335           AC135635.5         110093-110205
            1336-1383           AC135635.5         110746-110793
            1384-1441           AC135635.5         111475-111532
            1442-2978           AC135635.5         112848-114384
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 31.07 cM"
     gene            1..2978
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /note="major facilitator superfamily domain containing 1"
                     /db_xref="GeneID:66868"
                     /db_xref="MGI:MGI:1914118"
     exon            1..210
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1445
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /note="major facilitator superfamily domain-containing
                     protein 1"
                     /codon_start=1
                     /product="lysosomal dipeptide transporter MFSD1"
                     /protein_id="NP_080089.1"
                     /db_xref="CCDS:CCDS38450.1"
                     /db_xref="GeneID:66868"
                     /db_xref="MGI:MGI:1914118"
                     /translation="MEDEDGEDRALLGGRREADSAVHGAPRALSALCDPSRLAHRLVV
                     LSLMCFLGFGSYFCYDNPAALQTQVKRDMQVNTTKFMLLYAWYSWPNVVLCFLGGFLI
                     DRIFGIRWGTVIFSCFVCIGQVIFALGGIFNAFWLMELGRFVFGIGGESLAVAQNTYA
                     VSWFKGKELNLVFGLQLSMARIGSTVNMNLMGWLYGKIEALLGSAGHMTLGVTLMIGC
                     ITCIFSLICALALAYLDRRAEKILHKEQGKTGEVIKLRDIKDFSLPLILVFVICVCYY
                     VAVFPFIGLGKVFFMEKFRFSSQSASAINSIVYIISAPMSPLFGLLVDKTGKNIIWVL
                     YAVAATLVSHMMLAFTFWNPWIAMCLLGFSYSLLACALWPMVAFIVPEHQLGTAYGFM
                     QSIQNLGLAVIAILAGMILDSKGYLLLEVFFIACVSLSLLAVVCLYLVNRAQGGNLNY
                     SAKQRERMKLSHPE"
     exon            211..263
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            264..376
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            377..419
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            420..487
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            488..596
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            597..699
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            700..798
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            799..910
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            911..967
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1124
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1222
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1335
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1336..1383
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1384..1441
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..2978
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1815..1820
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1839
                     /gene="Mfsd1"
                     /gene_synonym="1200003O06Rik"
                     /note="major polyA site"
ORIGIN      
        1 gccgctgccg ccctgccttt gcttcctgct ccgttccagg ctgcaagctg atggaggacg
       61 aggatgggga agatcgggcg ctgcttgggg gccgccgcga ggcggacagc gcagtccatg
      121 gcgcccctcg cgctctgtcc gcgctctgcg accccagccg cctggcgcac cggctcgtgg
      181 tgctgtcgct catgtgcttc cttggcttcg gcagctattt ctgctatgac aaccccgctg
      241 cccttcagac tcaggtgaaa cgggacatgc aagtgaacac cacgaaattc atgctgctgt
      301 atgcctggta ttcttggccc aatgtagttt tgtgtttctt gggtggcttt ttgatagacc
      361 gcatatttgg aatacgatgg ggcacagtta tttttagctg ctttgtttgc atcggacagg
      421 ttatatttgc tctgggtggt atatttaatg cattttggct gatggagctt gggaggtttg
      481 tgtttgggat tggtggagag tccttagcag ttgcccagaa tacatatgct gtgagttggt
      541 ttaaaggcaa agaattaaac ctggtgtttg gacttcaact cagcatggcc agaattggaa
      601 gcacagtgaa tatgaacctc atgggatggc tgtatgggaa gattgaagca ttactgggct
      661 cagctggcca catgaccctg ggcgtgacac ttatgattgg gtgtataacg tgcatttttt
      721 cactaatctg tgctttggct cttgcttatt tggatcggag agcagagaaa atacttcata
      781 aagaacaagg gaaaacaggt gaagtcatta agctgaggga tataaaggac ttctcgttac
      841 ccctgatcct ggtcttcgtc atctgtgtct gctactacgt ggcggtgttc ccgttcatcg
      901 ggctgggcaa agttttcttc atggagaaat tcagattctc ttcccagtca gcaagtgcaa
      961 ttaacagtat cgtgtatatc atatcagctc ccatgtctcc attatttgga ctcttggtgg
     1021 ataaaacagg caagaacatc atctgggtcc tgtatgcggt ggccgccacc ctcgtgtccc
     1081 acatgatgct ggccttcacc ttttggaacc catggattgc tatgtgtctc ctggggttct
     1141 cctactcatt gctggcctgt gcattgtggc caatggtggc attcatagtt cctgaacacc
     1201 agctggggac tgcatatgga ttcatgcagt ctattcagaa cctcgggctg gccgtcatcg
     1261 ctatccttgc tggcatgatc ctggactcga agggatactt gctgctggag gtgttcttca
     1321 ttgcctgtgt ttctttgtca ctgctggctg tggtctgtct gtacttggtg aaccgtgccc
     1381 aaggagggaa tctaaattat tctgcaaaac aaagggaacg gatgaagctc tctcatccag
     1441 agtaaagccg tgagaaggga tagcattcgc ggctgactgg aaaccttcag gaagacttcc
     1501 ttttttaaaa cgtcatgtag aaaaatgata gaacggaaaa tatatttata tttgaaatat
     1561 gcctacttaa agtgattcat gcagactatc ttagcctttc ataaccatgt gttgtggaag
     1621 gtttgtttct ggtcgtgaac agtgaacagt ttacggaatc tggaacattc aggcaaagac
     1681 ctctgtaagg atctgcatct tctgctggga cgatttctaa taagggcgtc ctgcggatga
     1741 agcctttcct ttgtattata gcaggaaaaa gccacaaaac aggagggtct gtggtagaag
     1801 gctgttacat acataataaa gaatgacatt ttaaccgcac tcactgtgtc ctagagaaca
     1861 atatgtaatt gctccatatt tccctctagg agattatgtg tgaatgtcgt tagtgatggc
     1921 tgctgagcag caagcctttg gaagtttttt ttttttcctt aaagacaata aaagtgtcct
     1981 tcagtttatg atggctacct atatacatgg atccataaaa ccaatgcaaa aggtagactt
     2041 gatgcctgcc ttatattttt acaacattgc aataacaact tcaagtttcc tgtggaagat
     2101 aatttccaga aatcaaatct gcaataaaga aaatgtccta gatccaatct cattgaatag
     2161 ttttataggt gtaaggtacg tggggtacat agccttgtat gcttcaagct ttggcaccaa
     2221 gggctggaga gatgactcgg tggttaagaa catttactgc tctttaagag gaccccaggt
     2281 cagttttcag catccacatg gggccactca tagtcaccta actccagctc ccagggatcc
     2341 agtgccctgc tgaatcttaa gcagtacaga aacagtttga aaatgcattc cttttctttt
     2401 tggtttatag tttgttatgc attcttcact tgtgagcttc gaggctttga ctacatgtga
     2461 ctctcatagc acatgtcaag tatatgaact ggcccctctg cactggtcct gtcttcccac
     2521 tgtgtccaca tgaggtgtgg ggagccttga catgcttatg aatgggctgg gtgcctgctc
     2581 ggctcacagg gttgtccatc tcgatttcct tctcttgagt gtattctcta gtgcacactc
     2641 taacctggtt ccatctgact taaacaaaaa ccgtagtttc cagctcagaa tcatcctaac
     2701 atagaaactt ctctaggcca ctcaggctgt atacaattaa tgatacctat gaactttaaa
     2761 gcctcatcct gtgccttttt agctcacttt gatttgtcag gaatttgtgg cattgtgggc
     2821 ttggggtgta tttgatcgtc cttagattgt cagctccatg agagcaggga cattgcctgg
     2881 tttgttagtg actctatcct agtgcctagc ataatgaatg tacaaaactt tttctttctc
     2941 ctttttgtat aatgtataaa ataaaaagta attggaac
//
