LOCUS       NM_133201               4476 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus mitofusin 2 (Mfn2), transcript variant 2, mRNA.
ACCESSION   NM_133201 XM_904980 XM_919108 XM_919115 XM_919124 XM_919135
            XM_919144 XM_919157 XM_919164
VERSION     NM_133201.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Xu,M.X., Xu,T. and An,N.
  TITLE     Suppression of miR-17 Alleviates Acute Respiratory
            Distress-associated Lung Fibrosis by Regulating Mfn2
  JOURNAL   Curr Med Sci 44 (5), 964-970 (2024)
   PUBMED   39446286
  REMARK    GeneRIF: Suppression of miR-17 Alleviates Acute Respiratory
            Distress-associated Lung Fibrosis by Regulating Mfn2.
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Zhang,Y., Ma,L., Wang,Z., Gao,C., Yang,L., Li,M., Tang,X., Yuan,H.,
            Pang,D. and Ouyang,H.
  TITLE     Mfn2R364W, Mfn2G176S, and Mfn2H165R mutations drive
            Charcot-Marie-Tooth type 2A disease by inducing apoptosis and
            mitochondrial oxidative phosphorylation damage
  JOURNAL   Int J Biol Macromol 278 (Pt 1), 134673 (2024)
   PUBMED   39142491
  REMARK    GeneRIF: Mfn2[R364W], Mfn2[G176S], and Mfn2[H165R] mutations drive
            Charcot-Marie-Tooth type 2A disease by inducing apoptosis and
            mitochondrial oxidative phosphorylation damage.
REFERENCE   3  (bases 1 to 4476)
  AUTHORS   Lu,L., Ning,Y., Gu,F., Lin,Z., Qin,Y., Feng,L., Tang,M. and Cao,Y.
  TITLE     The circular RNA circSLC16A10 alleviates diabetic retinopathy by
            improving mitochondrial function via the miR-761-5p/MFN2 axis
  JOURNAL   Cell Signal 121, 111283 (2024)
   PUBMED   38960059
  REMARK    GeneRIF: The circular RNA circSLC16A10 alleviates diabetic
            retinopathy by improving mitochondrial function via the
            miR-761-5p/MFN2 axis.
REFERENCE   4  (bases 1 to 4476)
  AUTHORS   Kam,M.K., Jung,S.M., Lee,G.E., Lee,S.W., Lee,H.J., Park,Y.H. and
            Lee,D.S.
  TITLE     Mitofusin 1 and 2 overexpression reduces AbetaO-mediated ER stress
            and apoptosis in N2a APPswe cells
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 8-14 (2024)
   PUBMED   39097901
  REMARK    GeneRIF: Mitofusin 1 and 2 overexpression reduces AbetaO-mediated
            ER stress and apoptosis in N2a APPswe cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4476)
  AUTHORS   Liu,D., Liao,P., Li,H., Tong,S., Wang,B., Lu,Y., Gao,Y., Huang,Y.,
            Zhou,H., Shi,L., Papadimitriou,J., Zong,Y., Yuan,J., Chen,P.,
            Chen,Z., Ding,P., Zheng,Y., Zhang,C., Zheng,M. and Gao,J.
  TITLE     Regulation of blood-brain barrier integrity by Dmp1-expressing
            astrocytes through mitochondrial transfer
  JOURNAL   Sci Adv 10 (26), eadk2913 (2024)
   PUBMED   38941455
REFERENCE   6  (bases 1 to 4476)
  AUTHORS   Chen,K.H., Guo,X., Ma,D., Guo,Y., Li,Q., Yang,D., Li,P., Qiu,X.,
            Wen,S., Xiao,R.P. and Tang,J.
  TITLE     Dysregulation of HSG triggers vascular proliferative disorders
  JOURNAL   Nat Cell Biol 6 (9), 872-883 (2004)
   PUBMED   15322553
REFERENCE   7  (bases 1 to 4476)
  AUTHORS   Koshiba,T., Detmer,S.A., Kaiser,J.T., Chen,H., McCaffery,J.M. and
            Chan,D.C.
  TITLE     Structural basis of mitochondrial tethering by mitofusin complexes
  JOURNAL   Science 305 (5685), 858-862 (2004)
   PUBMED   15297672
  REMARK    GeneRIF: results suggest that the heptad repeat region (HR2) of
            mfn1 functions as a mitochondrial tether before fusion
REFERENCE   8  (bases 1 to 4476)
  AUTHORS   Bach,D., Pich,S., Soriano,F.X., Vega,N., Baumgartner,B., Oriola,J.,
            Daugaard,J.R., Lloberas,J., Camps,M., Zierath,J.R.,
            Rabasa-Lhoret,R., Wallberg-Henriksson,H., Laville,M., Palacin,M.,
            Vidal,H., Rivera,F., Brand,M. and Zorzano,A.
  TITLE     Mitofusin-2 determines mitochondrial network architecture and
            mitochondrial metabolism. A novel regulatory mechanism altered in
            obesity
  JOURNAL   J Biol Chem 278 (19), 17190-17197 (2003)
   PUBMED   12598526
REFERENCE   9  (bases 1 to 4476)
  AUTHORS   Chen,H., Detmer,S.A., Ewald,A.J., Griffin,E.E., Fraser,S.E. and
            Chan,D.C.
  TITLE     Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion
            and are essential for embryonic development
  JOURNAL   J Cell Biol 160 (2), 189-200 (2003)
   PUBMED   12527753
REFERENCE   10 (bases 1 to 4476)
  AUTHORS   Rojo,M., Legros,F., Chateau,D. and Lombes,A.
  TITLE     Membrane topology and mitochondrial targeting of mitofusins,
            ubiquitous mammalian homologs of the transmembrane GTPase Fzo
  JOURNAL   J Cell Sci 115 (Pt 8), 1663-1674 (2002)
   PUBMED   11950885
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY131209.1, AL607066.23 and AK042080.1.
            
            On Oct 25, 2013 this sequence version replaced NM_133201.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All variants (1 through 7) encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046503.1, AK042080.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                BY131209.1         2-76
            76-220              AL607066.23        114354-114498       c
            221-4462            AK042080.1         2-4243
            4463-4476           AL607066.23        83250-83263         c
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 78.56 cM"
     gene            1..4476
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /note="mitofusin 2"
                     /db_xref="GeneID:170731"
                     /db_xref="MGI:MGI:2442230"
     exon            1..252
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            253..395
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            396..574
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     CDS             400..2673
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /note="hypertension related protein 1; HSG protein;
                     transmembrane GTPase MFN2; mitochondrial assembly
                     regulatory factor"
                     /codon_start=1
                     /product="mitofusin-2"
                     /protein_id="NP_573464.2"
                     /db_xref="CCDS:CCDS38965.1"
                     /db_xref="GeneID:170731"
                     /db_xref="MGI:MGI:2442230"
                     /translation="MSLLFSRCNSIVTVKKDKRHMAEVNASPLKHFVTAKKKINGIFE
                     QLGAYIQESASFLEDTHRNTELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFF
                     GRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVGGTDGHEAFLLTEGSEEKKSVKTV
                     NQLAHALHQDEQLHAGSMVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCL
                     DADVFVLVANSESTLMQTEKQFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQ
                     HMERCTSFLVDELGVVDRAQAGDRIFFVSAKEVLSARVQKAQGMPEGGGALAEGFQVR
                     MFEFQNFERQFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHIAAQEQRVYCLE
                     MREERQDRLRFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDEYQM
                     DFHPSPVVLKVYKNELHRHIEEGLGRNLSDRCSTAIASSLQTMQQDMIDGLKPLLPVS
                     MRNQIDMLVPRQCFSLSYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRAL
                     LGYSDQVQRPLPLTPANPSMPPLPQSSLTQEELMVSMVTGLASLTSRTSMGILVVGGV
                     VWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEYASEKLQLIISYTGS
                     NCSHQVQQELSGTFAHLCQQVDITRDNLEQEIAAMNKKVEALDSLQSRAKLLRNKAGW
                     LDSELNMFTHQYLQPSR"
     exon            575..710
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            711..873
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            874..998
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            999..1107
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1215
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1369
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1437
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1438..1559
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1686
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1791
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1894
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2115
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            2116..2271
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2468
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            2469..2603
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..4476
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4440..4445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /note="hexamer: AATAAA"
     polyA_site      4463
                     /gene="Mfn2"
                     /gene_synonym="D630023P19Rik; Fzo"
                     /note="major polyA site"
ORIGIN      
        1 agcttacgtc atctgcacga gacacgggac ggttaccagg cacgcggggg ctccttttgg
       61 agcgcggagt tcctagacac tgtggtatag gagtggtaag gttgaggtga cagcgttcag
      121 aggccatcgg ctcgtccggg ctgcagcggg gcgagtgacg agcgcagccc cctcccctcc
      181 cgcttctctc ggtgtggcgc cggctagtga cgtgttgggt gtgatggccg ccgcgaggcc
      241 gggaaggtga agaagcttgg acaggtggag tcaacaccat caggggccaa cctggacctg
      301 aatcagcaca gaggagacct cgagtccttt tcatctttct gactccagcc atgtccacga
      361 tgcccaacct gtgaaggtct cttaccagct agaaacgaga tgtccctgct cttttctcga
      421 tgcaactcca tcgtcaccgt caagaaggat aagcgacaca tggctgaagt gaatgcttcc
      481 cctctcaagc actttgtcac tgccaagaaa aagatcaatg gaatctttga gcagctgggg
      541 gcctacatcc aagagagcgc cagcttcctt gaagacaccc acaggaacac agaactggac
      601 ccggttacca cggaagagca ggtcctggac gtcaaagggt acctgtccaa ggtcaggggt
      661 atcagcgaag tgctggccag gcggcacatg aaggtggctt tttttggccg gacgagcaat
      721 gggaagagca ccgtgatcaa tgccatgctc tgggacaaag ttctgccatc tgggattggt
      781 cataccacca attgcttcct gcgggttggg ggcacagatg gccatgaggc cttcctcctc
      841 acagagggct cagaagagaa gaagagtgtc aagactgtga accaactggc ccatgccctc
      901 catcaggacg agcagttgca tgcaggcagc atggtgagtg tgatgtggcc caactccaag
      961 tgtccgctcc tgaaggatga cctcgtgctg atggacagcc ctgggatcga tgttaccacg
     1021 gagctggaca gctggattga taagttttgc ctggatgctg atgtgtttgt gctggtggcc
     1081 aactcagagt ccacgctgat gcagacggag aagcagttct tccacaaagt gagtgaacgt
     1141 ctctcccggc ccaacatctt catcctgaac aaccgctggg atgcgtctgc ctcggagcct
     1201 gagtacatgg aggaggtgcg gcggcagcac atggagcgct gcaccagctt tctggtggat
     1261 gagctgggcg tggtggatcg agctcaggct ggggaccgga tcttcttcgt gtctgccaag
     1321 gaggttctca gcgccagggt ccagaaagcc cagggcatgc cagaaggagg cggcgctctc
     1381 gcagaaggtt ttcaagtgag gatgtttgag tttcagaatt tcgagaggca gtttgaggag
     1441 tgcatttccc agtctgcagt aaagaccaaa tttgagcagc acacagtccg ggccaagcag
     1501 attgcagagg ccgttcgtct catcatggat tccctgcaca tcgcagctca ggagcagcgg
     1561 gtttattgcc tagaaatgcg ggaagagcgg caagaccggc tgaggtttat tgacaagcag
     1621 ctggagctcc tggctcaaga ctacaagctg cgaattaagc agattacgga ggaagtggaa
     1681 aggcaggtgt ccacagccat ggctgaagag atcaggcgcc tctctgtgct agttgacgag
     1741 taccagatgg acttccaccc atccccagtt gtcctcaagg tttataagaa cgagctgcac
     1801 cgccatatag aggaaggtct gggccggaac ctgtctgacc gctgctccac tgccattgcc
     1861 agttcactgc agactatgca gcaggacatg atagacggct tgaagcccct tcttcctgta
     1921 tctatgcgga atcagataga catgctggtc cctcgacagt gtttctccct cagctatgac
     1981 ctgaattgtg acaagctgtg tgctgacttt caggaggaca tcgagttcca cttctccctt
     2041 ggatggacta tgctagtgaa caggttcctg ggccccaaga atagccgccg ggccttgcta
     2101 ggctacagtg atcaggttca gcgtcctctc cctctgacac ctgccaaccc cagcatgccc
     2161 cccttgccac agagctccct cacccaggag gagctcatgg tctccatggt tactggcctg
     2221 gcctctctga cgtctaggac ctccatgggc attcttgtgg tcggaggagt ggtgtggaag
     2281 gcagtgggct ggagactcat cgccctctcc tttggactgt atggcctcct gtacgtctat
     2341 gagcgactga cctggaccac caaagccaaa gagagggcct tcaagcgcca gtttgtggaa
     2401 tacgccagtg agaagctaca gctcatcatc agttacaccg gctctaactg cagccaccaa
     2461 gtccagcagg aattgtctgg gacatttgct catctgtgcc agcaagttga catcacccga
     2521 gataatctgg agcaggaaat tgctgccatg aacaagaaag tcgaggctct ggattcactt
     2581 cagagcagag ccaaactgct caggaataaa gctggctggt tggacagcga actcaacatg
     2641 ttcacacacc agtacctgca gcccagcaga tagtgggcag ccagggcgga cctgcacgaa
     2701 gaagaggcag ggccgcacct cccatcagct ctagtccttg gccgctgcag agagaaggaa
     2761 agcacccagt cttgtaccag ttactcccta ccccctgcag gaagacccct ggctcatacc
     2821 ctaatggaga ccaacaagga ctggacagct cggctccaag gagttatgca tgtgtctgtg
     2881 tctgctcctc acgctcaccc ggttgaaagt cactgtgcat ttgataaagt tttcctcaga
     2941 atggcaccct gcagaatgac tgatgggcgc cttcacaggc atcaggcagc cactcctact
     3001 ttctccagcc tgagcacacc tacagagaga cagtgttggg ggtgacagga tgccccagaa
     3061 aaattcagga tacttctgaa attgccatgt ttgtctcaga acttgtatct tgcgtttggc
     3121 cctcagcttt agcacttttt cccacttgca gagtctttca gttgtgcttg gtagccaggc
     3181 tcaacacacc ggggctttag actcaagcag cttatatggg aagctggaga cccctgccag
     3241 ccagagcaca gtaagggctc ggagaaggta tgtgaactag ggtatctgat gtggtagtgc
     3301 agttggtgtc tggcattgtg acgacaaggg acttgcctcc cttcttgcca ctgaggccct
     3361 tgcttttcct tcagctcttg gcctgttggt ccggtttatt agtgagagct agtttgctcc
     3421 aggtggttaa aggatgtagc aggaggaatg gtggaagatg ggagctgccc gggttagtac
     3481 agaagaggtc tgtctatctg acctagcact gaggggttca caaacctgtg aggtgcacct
     3541 cactaatttt ggctcatccc tgccttgcta caagctccct gtggccacca ttctctggac
     3601 tgtaccgtgc tgatgactgc agctggctac aaaacagcat gagaacctgg ccgctgttct
     3661 cctactgcct ccaccctcag gcaggcctcc tcctcttctg cccctgaagc ctcctgctgc
     3721 ttgctcctcc tgcctccacc ctcaggcagg cctcctcctc ttctgcccct gaagcctcct
     3781 gctgctgctc ctccacctca gtcagtgctg aacttctcac tgttgagtgt tgaaattgtt
     3841 aatggtcaaa gcagggagga tgccagtcac caaggttgat tgtcttggga aagcagtttt
     3901 agagtgatga atcctttgta ggacaggtag agagtatctt agagataaaa aaatgggcta
     3961 tgtcctctcc tgcccatgtc ttgtgctcac tcgcccctca gtctaccctt tttgctggga
     4021 cagtgatggt cttggccaag gtcagcatgt aatggaaaga cagtgtattg ccaggctgga
     4081 tagatggggt catctcacag tgggtcacgt gaaaatcagt gtcacgtagt cccagtgaat
     4141 gttgtgttct ttctgggggg aggggggaat agaataaact caaattcttt ttcagtatag
     4201 cccctgagta atgaatgaaa atttgagctt atgtaagtaa agattcattt atgccaccag
     4261 ggagagcctg tatctgagag acatttagca gagagcactt tagttttttg ctgctaactg
     4321 ttgtgtgatc ctttgctggg gtgcaaaagg caccggacac agtgtgtttg cttctggtat
     4381 atttaggtga tgcatccgcc tgggttacct gtatctctgc tgatcatatt ggaaactaaa
     4441 ataaagcccc atttgtgacc ctgtgtgagc tctctg
//
