LOCUS       NM_024200               4550 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Mus musculus mitofusin 1 (Mfn1), mRNA.
ACCESSION   NM_024200
VERSION     NM_024200.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Thomas,D., Yang,J., Cho,S.J. and Stout-Delgado,H.
  TITLE     Role of Mitofusin 1 in mediating reactive oxygen species in
            alveolar macrophages during Streptococcuspneumoniae
  JOURNAL   Redox Biol 76, 103329 (2024)
   PUBMED   39197317
  REMARK    GeneRIF: Role of Mitofusin 1 in mediating reactive oxygen species
            in alveolar macrophages during Streptococcuspneumoniae.
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Kam,M.K., Jung,S.M., Lee,G.E., Lee,S.W., Lee,H.J., Park,Y.H. and
            Lee,D.S.
  TITLE     Mitofusin 1 and 2 overexpression reduces AbetaO-mediated ER stress
            and apoptosis in N2a APPswe cells
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 70 (7), 8-14 (2024)
   PUBMED   39097901
  REMARK    GeneRIF: Mitofusin 1 and 2 overexpression reduces AbetaO-mediated
            ER stress and apoptosis in N2a APPswe cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4550)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4550)
  AUTHORS   Li,C., Zhang,Y., Shen,J., Bao,H., Zhao,Y., Li,D., Li,S., Liu,Y.,
            Yang,J., Zhou,Z., Gao,K., Zhao,L., Pei,Y., Lu,Y., Pan,Z. and Cai,B.
  TITLE     Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3
            of Structural, Metabolic, and Contractile Related Genes
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305992 (2024)
   PUBMED   38196272
REFERENCE   5  (bases 1 to 4550)
  AUTHORS   Ding,Y., He,Z., Sha,Y., Kee,K. and Li,L.
  TITLE     Eif4enif1 haploinsufficiency disrupts oocyte mitochondrial dynamics
            and leads to subfertility
  JOURNAL   Development 150 (23) (2023)
   PUBMED   38088064
REFERENCE   6  (bases 1 to 4550)
  AUTHORS   Chen,H., Chomyn,A. and Chan,D.C.
  TITLE     Disruption of fusion results in mitochondrial heterogeneity and
            dysfunction
  JOURNAL   J Biol Chem 280 (28), 26185-26192 (2005)
   PUBMED   15899901
  REMARK    GeneRIF: OPA1, Mfn1 and Mfn2 have roles in mitochondrial fusion,
            cell growth, mitochondrial membrane potential, and cellular
            respiration
REFERENCE   7  (bases 1 to 4550)
  AUTHORS   Cipolat,S., Martins de Brito,O., Dal Zilio,B. and Scorrano,L.
  TITLE     OPA1 requires mitofusin 1 to promote mitochondrial fusion
  JOURNAL   Proc Natl Acad Sci U S A 101 (45), 15927-15932 (2004)
   PUBMED   15509649
REFERENCE   8  (bases 1 to 4550)
  AUTHORS   Koshiba,T., Detmer,S.A., Kaiser,J.T., Chen,H., McCaffery,J.M. and
            Chan,D.C.
  TITLE     Structural basis of mitochondrial tethering by mitofusin complexes
  JOURNAL   Science 305 (5685), 858-862 (2004)
   PUBMED   15297672
  REMARK    GeneRIF: results suggest that the heptad repeat region (HR2) of
            mfn1 functions as a mitochondrial tether before fusion
REFERENCE   9  (bases 1 to 4550)
  AUTHORS   Chen,H., Detmer,S.A., Ewald,A.J., Griffin,E.E., Fraser,S.E. and
            Chan,D.C.
  TITLE     Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion
            and are essential for embryonic development
  JOURNAL   J Cell Biol 160 (2), 189-200 (2003)
   PUBMED   12527753
REFERENCE   10 (bases 1 to 4550)
  AUTHORS   Rojo,M., Legros,F., Chateau,D. and Lombes,A.
  TITLE     Membrane topology and mitochondrial targeting of mitofusins,
            ubiquitous mammalian homologs of the transmembrane GTPase Fzo
  JOURNAL   J Cell Sci 115 (Pt 8), 1663-1674 (2002)
   PUBMED   11950885
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC130281.13.
            
            On Oct 4, 2022 this sequence version replaced NM_024200.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK220169.1, AK141611.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-223               AC130281.13        89594-89816         c
            224-342             AC130281.13        87273-87391         c
            343-478             AC130281.13        84916-85051         c
            479-641             AC130281.13        80857-81019         c
            642-766             AC130281.13        76359-76483         c
            767-875             AC130281.13        75114-75222         c
            876-983             AC130281.13        72594-72701         c
            984-1137            AC130281.13        64981-65134         c
            1138-1205           AC130281.13        63748-63815         c
            1206-1327           AC130281.13        57703-57824         c
            1328-1454           AC130281.13        56151-56277         c
            1455-1559           AC130281.13        55810-55914         c
            1560-1662           AC130281.13        55394-55496         c
            1663-1892           AC130281.13        54984-55213         c
            1893-2045           AC130281.13        50880-51032         c
            2046-2242           AC130281.13        49605-49801         c
            2243-2377           AC130281.13        44921-45055         c
            2378-4550           AC130281.13        40054-42226         c
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 15.75 cM"
     gene            1..4550
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /note="mitofusin 1"
                     /db_xref="GeneID:67414"
                     /db_xref="MGI:MGI:1914664"
     exon            1..223
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            224..342
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     CDS             231..2456
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /note="transmembrane GTPase MFN1"
                     /codon_start=1
                     /product="mitofusin-1"
                     /protein_id="NP_077162.2"
                     /db_xref="CCDS:CCDS17296.1"
                     /db_xref="GeneID:67414"
                     /db_xref="MGI:MGI:1914664"
                     /translation="MAETVSPLKHFVLAKKAITAIFGQLLEFVTEGSHFVEATYRNPE
                     LDRIASEDDLVEIQGYRNKLAVIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLP
                     SGIGHTTNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVHV
                     FWPKAKCALLRDDLVLVDSPGTDVTTELDIWIDKFCLDADVFVLVANSESTLMNTEKH
                     FFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVSPSEA
                     RNRIFFVSAKEVLNSRKHKAQGMPEGGGALAEGFQARLQEFQNFEQTFEECISQSAVK
                     TKFEQHTIRAKQILDTVKNILDSVNVAAAEKRVYSMEEREDQIDRLDFIRNQMNLLTL
                     DVKKKIKEVTEEVANKVSCAMTDEICRLSVLVDEFCSEFHPTPSVLKVYKSELNKHIE
                     DGMGRNLADRCTNEVNASILQSQQEIIENLKPLLPAGIQNKLHTLIPCKKFDLSYDLN
                     CHKLCSDFQEDIVFRFSLGWSSLVHRFLGSTNAQRVLLGLSEPIFQVPRSLASTPTAP
                     SNPAAPDNAAQEELMITLITGLASLTSRTSMGIIVVGGVIWKTVGWKLISVTLSMYGA
                     LYLYERLTWTTRAKERAFKQQFVNYATEKLQMIVSFTSANCSHQVQQEMATTFARLCQ
                     QVDVTQKHLEEEIARLSKEIDQLEKIQNNSKLLRNKAVQLESELENFSKQFLHPSSGE
                     S"
     exon            343..478
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            479..641
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            642..766
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            767..875
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            876..983
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1137
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1205
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1327
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1454
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1559
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..1662
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1892
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2045
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2242
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2377
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..4550
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2971..2976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /note="hexamer: ATTAAA"
     polyA_site      2994
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /note="major polyA site"
     regulatory      4521..4526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
                     /note="hexamer: AATAAA"
     polyA_site      4550
                     /gene="Mfn1"
                     /gene_synonym="2310002F04Rik; 6330416C07Rik; D3Ertd265e;
                     HR2; mKIAA4032"
ORIGIN      
        1 cctcccctct ttcgggagga tgagacagcc agggggctgt ggcggagctg tggttgctgg
       61 gtgacagtgg ggagcaggga cggagtgagt gtccgctggc gggtggcgac gtccggggtg
      121 accttcgagc tccggaccgg cgcagcgcga gcgccctgac gggccggggc ggcgggccgg
      181 gcaagggcga cgggcggcca gcctgaggga aggccctgtc caggtgcata atggcagaaa
      241 cggtatctcc actgaagcac tttgtgctgg caaagaaagc catcactgca atcttcggcc
      301 agttactgga gtttgttact gagggctcac attttgttga agcaacatac aggaatccag
      361 aacttgatcg aatagcatcc gaggatgatc tggtggaaat acagggctac agaaacaagc
      421 ttgctgtcat tggggaggtg ctgtctcgga gacatatgaa ggtggcattt tttggcagga
      481 caagtagtgg caagagctct gtcatcaatg caatgctgtg ggataaagtc ctccccagcg
      541 ggattggtca cacaaccaac tgcttcctga gtgtcgaggg gaccgatgga gataaagcct
      601 accttatgac cgaagggtca gatgaaaaga aaagtgtgaa gactgttaat cagctggccc
      661 atgccctcca tatggataaa gacttgaaag ctggctgtct tgtgcatgta ttttggccca
      721 aagcaaaatg tgccctcttg agagatgacc tggttttagt agacagccca ggtacagatg
      781 tcaccacaga gctggatatc tggattgata agttttgcct tgatgctgat gtctttgttt
      841 tggttgcaaa ctcggaatca acactgatga acacggagaa acattttttc cataaggtga
      901 atgagcggct ctccaagccc aacatcttca ttctgaataa ccgttgggat gcttctgctt
      961 cggagccgga gtacatggag gatgtgcgca gacagcacat ggagagatgt cttcacttct
     1021 tggtagaaga gctcaaggtt gtaagtccgt cggaagctcg gaatcggatc ttttttgttt
     1081 cagccaagga agttctcaac tccagaaagc ataaagctca ggggatgcca gaaggtggtg
     1141 gggcacttgc agaaggattt caagcaagat tacaggagtt tcaaaatttt gaacaaactt
     1201 ttgaggagtg tatctcgcag tcagcagtga aaacaaagtt tgaacagcac actatcagag
     1261 ctaaacagat actagacact gtgaaaaaca tactggactc agtaaacgtg gcagcagcag
     1321 agaagagggt ttattcaatg gaagagaggg aagaccaaat cgatagactg gactttatcc
     1381 gaaaccagat gaacctttta acactggatg ttaagaagaa gatcaaggag gtcacggagg
     1441 aggtggcaaa caaggtttct tgtgcaatga cagatgaaat ttgtcgacta tctgttttgg
     1501 ttgatgagtt ttgttctgag tttcatccta cccccagtgt actgaaagtg tataagagtg
     1561 agttaaataa gcacatagaa gatggcatgg gaagaaattt ggctgatcgg tgtaccaatg
     1621 aagtcaatgc ctccattctt caatctcagc aagaaatcat cgaaaacttg aagccactac
     1681 ttccagctgg tatacagaat aaacttcata cattaatccc ttgcaaaaag tttgacctca
     1741 gctatgatct caattgccac aagctgtgtt cggattttca agaggacatt gtgtttcggt
     1801 tttccctggg ctggtcttcc cttgtacatc gattcctggg ttccacaaat gcacagaggg
     1861 tgctgctcgg gctgtcagag cccatctttc aggtccctag atctttagct tcaactccta
     1921 ctgctccttc taacccagca gccccggata atgcagccca ggaggagctc atgatcaccc
     1981 tgatcacagg attggcgtcc ctcacgtcga gaacctccat gggcatcatc gttgttgggg
     2041 gcgtgatttg gaaaacagtg ggctggaaac taatctctgt caccttaagt atgtacggag
     2101 ctctgtacct ttatgagagg ctgacgtgga cgacccgtgc gaaagagaga gcgtttaagc
     2161 agcagtttgt aaactatgca accgagaagc tgcagatgat tgtgagcttc accagtgcaa
     2221 actgcagcca ccaagtacag caagaaatgg ccactacttt tgctcgactg tgccaacaag
     2281 ttgatgttac tcagaaacat ctggaagagg aaattgcaag attatccaaa gagatagacc
     2341 aactggagaa aatacagaac aactcaaagc tcttaagaaa taaagctgtt caacttgaaa
     2401 gtgagctgga gaatttttcg aagcagtttc tacacccgag cagtggagaa tcctaacggc
     2461 agaggcactg taggaggaag cggacttgga agatgggaaa tgttacttta tgaaatgacc
     2521 tcagtacaag ttactaactc ttagtatcga tgccttgcgg agattgtggt aatgacctgt
     2581 ctcaggggtt gcacctttgg aagtgttgtg attcgccttg tcttagcatt agtttggagt
     2641 aaagactgaa ttgttaaggt taaatgatga attcctttag aaacagtgga accggctgtg
     2701 cggcccctga gggtgggtcc tgcagctcct caccaggctg gctgtctgcg gctctcagaa
     2761 gctgcttcct ggcatccagg agttagagac cttttcatcc tttctcagtg ctagttcttg
     2821 atgcttcttt aatgggaata gtgaacttgt ttataagccg atttgctcaa accaggggtg
     2881 tgggctgctc ctgggggggt cctgcaatca ctctgtcctc acagcaagga tgtaaccact
     2941 actaaacagt ttttactttc ttttattccc attaaagctg atgtgaaata gtaacgaggc
     3001 ctatcattgt agccacgcat gaagtatttg ggtctgcttt cctgctctct ctctttagac
     3061 acattttcag ccttgagtcc agcaaggaga gcttttctgc ctaagtgtga agtggaaggt
     3121 ctgaaggggt gaaatgggag cagaaagtgt gtggagtgga gtggagtgtg gtgcagtgac
     3181 tgtggcaact ggttctcagc aggacactca ccgagaccct gggcgtgtcc aggcgtgtcc
     3241 ccactggaag ctttgatttg aattacatga atttcttggg ctctattaca aattaccact
     3301 ctgggttagt gacatgttta tagttcaggt attgatgttt gattgacagt ggtatggaaa
     3361 tgcttgtaaa tagcagacag gattattctc cacctgacaa tgaaaattaa agtgctaaca
     3421 ttggatcact tcaggaagtc agcagtgcca ccccaagggt tacccactgc agtgtcaaag
     3481 gtcagcactt caggcaggag cagaggagta tttagttaat atggccacat tgaggaaagg
     3541 ggcaaagaga tccagcaaac tcccagtatt tctttcctaa aggcctgtct tcacaatagg
     3601 cacacctatt ttttgtctgt ctgtttggtt gattagtttt gagacagggt ttctctgtgt
     3661 agccctggct atcctggaac taactcactt tgtagaccag gctggccttg aactcagaaa
     3721 tctgcctgcc tccagagtgc tgggattaaa ggcataccca gccctagatg cacctcttat
     3781 ttttaaaata cttgaagact attacaatgt tatatatcat tacttagacc atagagaagt
     3841 ttctaaaaac cacttaattt ttatgtagat tgggtgctgg gttgcagtat tcaaatttaa
     3901 atccatgtga ctaccttttg cttttattgc tgatttttag tcccccaaat ggtacaggca
     3961 accaagtggg actgggtgaa tagtacatga aaaagtagta tctagaattg aggtctgcta
     4021 acctaggtta cacagttact tggagcacat aattagccag ctacagcttg gttctctggg
     4081 tttgctcccg ggattgtcaa cattattttt aaacttgtaa agatttgtaa ggtgtagatt
     4141 ctcaacaagg tatgaatcaa gtttggggtc tgcagcaagc ttcaagtctg tcccagtgta
     4201 ctctgaagtc tgcaagccag tatctgtagt ccagaatgct ttcctcactg attaagaatg
     4261 tagagccagc tgatgaagtt cagaagttac gggtgttgag catggagtgt tgaaatgcag
     4321 acactagctc ttttaccaga caagagtgcg cctgccactg gtaggctcgt gcattacaga
     4381 ctgtaatcat gacggttact gattagttgc tacacacata cttgatgatt tactgtgtat
     4441 gtttacagta tttataaact ttgtatgatc ttaaactttt tacaaaacat ttttataagt
     4501 atgcaagctt gcaagatgaa aataaaccta tccgcctatt agttggtaaa
//
