LOCUS       NM_001045489            2032 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus milk fat globule EGF and factor V/VIII domain
            containing (Mfge8), transcript variant 2, mRNA.
ACCESSION   NM_001045489
VERSION     NM_001045489.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Mizote,Y., Inoue,T., Akazawa,T., Kunimasa,K., Tamiya,M.,
            Kumamoto,Y., Tsuda,A., Yoshida,S., Tatsumi,K., Ekawa,T., Honma,K.,
            Nishino,K. and Tahara,H.
  TITLE     Potent CTLs can be induced against tumor cells in an environment of
            lower levels of systemic MFG-E8
  JOURNAL   Cancer Sci 115 (4), 1114-1128 (2024)
   PUBMED   38332689
  REMARK    GeneRIF: Potent CTLs can be induced against tumor cells in an
            environment of lower levels of systemic MFG-E8.
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Dong,X., Zhang,Z., Shu,X., Zhuang,Z., Liu,P., Liu,R., Xia,S.,
            Bao,X., Xu,Y. and Chen,Y.
  TITLE     MFG-E8 Alleviates Cognitive Impairments Induced by Chronic Cerebral
            Hypoperfusion by Phagocytosing Myelin Debris and Promoting
            Remyelination
  JOURNAL   Neurosci Bull 40 (4), 483-499 (2024)
   PUBMED   37979054
  REMARK    GeneRIF: MFG-E8 Alleviates Cognitive Impairments Induced by Chronic
            Cerebral Hypoperfusion by Phagocytosing Myelin Debris and Promoting
            Remyelination.
REFERENCE   3  (bases 1 to 2032)
  AUTHORS   Datta,R., Mukherjee,D., Podolsky,M.J., Yang,C.D., Alba,D.L.,
            Singh,S., Arnold,T.D., Koliwad,S., Lizama,C.O. and Atabai,K.
  TITLE     PTP1B mediates the inhibitory effect of MFGE8 on insulin signaling
            through the beta5 integrin
  JOURNAL   J Biol Chem 300 (2), 105631 (2024)
   PUBMED   38199575
  REMARK    GeneRIF: PTP1B mediates the inhibitory effect of MFGE8 on insulin
            signaling through the beta5 integrin.
REFERENCE   4  (bases 1 to 2032)
  AUTHORS   Ding,P., Liu,J., Meng,Y., Wang,H., Huang,Y., Su,G., Xia,C., Du,X.,
            Dong,N., Cui,T., Zhang,J. and Li,J.
  TITLE     MFG-E8 facilitates heart repair through M1/M2 polarization after
            myocardial infarction by inhibiting CaMKII
  JOURNAL   Int Immunopharmacol 126, 111216 (2024)
   PUBMED   37977072
  REMARK    GeneRIF: MFG-E8 facilitates heart repair through M1/M2 polarization
            after myocardial infarction by inhibiting CaMKII.
REFERENCE   5  (bases 1 to 2032)
  AUTHORS   Sun,J., Hu,L., Bok,S., Yallowitz,A.R., Cung,M., McCormick,J.,
            Zheng,L.J., Debnath,S., Niu,Y., Tan,A.Y., Lalani,S., Morse,K.W.,
            Shinn,D., Pajak,A., Hammad,M., Suhardi,V.J., Li,Z., Li,N., Wang,L.,
            Zou,W., Mittal,V., Bostrom,M.P.G., Xu,R., Iyer,S. and
            Greenblatt,M.B.
  TITLE     A vertebral skeletal stem cell lineage driving metastasis
  JOURNAL   Nature 621 (7979), 602-609 (2023)
   PUBMED   37704733
REFERENCE   6  (bases 1 to 2032)
  AUTHORS   Aoki,N., Ishii,T., Ohira,S., Yamaguchi,Y., Negi,M., Adachi,T.,
            Nakamura,R. and Matsuda,T.
  TITLE     Stage specific expression of milk fat globule membrane
            glycoproteins in mouse mammary gland: comparison of MFG-E8,
            butyrophilin, and CD36 with a major milk protein, beta-casein
  JOURNAL   Biochim Biophys Acta 1334 (2-3), 182-190 (1997)
   PUBMED   9101712
REFERENCE   7  (bases 1 to 2032)
  AUTHORS   Collins,C., Nehlin,J.O., Stubbs,J.D., Kowbel,D., Kuo,W.L. and
            Parry,G.
  TITLE     Mapping of a newly discovered human gene homologous to the
            apoptosis associated-murine mammary protein, MFG-E8, to chromosome
            15q25
  JOURNAL   Genomics 39 (1), 117-118 (1997)
   PUBMED   9027496
REFERENCE   8  (bases 1 to 2032)
  AUTHORS   Ogura,K., Nara,K., Watanabe,Y., Kohno,K., Tai,T. and Sanai,Y.
  TITLE     Cloning and expression of cDNA for O-acetylation of GD3 ganglioside
  JOURNAL   Biochem Biophys Res Commun 225 (3), 932-938 (1996)
   PUBMED   8780713
REFERENCE   9  (bases 1 to 2032)
  AUTHORS   Aoki,N., Kishi,M., Taniguchi,Y., Adachi,T., Nakamura,R. and
            Matsuda,T.
  TITLE     Molecular cloning of glycoprotein antigens MGP57/53 recognized by
            monoclonal antibodies raised against bovine milk fat globule
            membrane
  JOURNAL   Biochim Biophys Acta 1245 (3), 385-391 (1995)
   PUBMED   8541316
REFERENCE   10 (bases 1 to 2032)
  AUTHORS   Stubbs,J.D., Lekutis,C., Singer,K.L., Bui,A., Yuzuki,D.,
            Srinivasan,U. and Parry,G.
  TITLE     cDNA cloning of a mouse mammary epithelial cell surface protein
            reveals the existence of epidermal growth factor-like domains
            linked to factor VIII-like sequences
  JOURNAL   Proc Natl Acad Sci U S A 87 (21), 8417-8421 (1990)
   PUBMED   2122462
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC110520.12, AK148371.1, AK087555.1 and CD776821.1.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            coding region compared to variant 1. The resulting protein (isoform
            2) is shorter but has the same N- and C-termini compared to isoform
            1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003892.1, AK148371.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AC110520.12        121808-121855       c
            49-533              AK148371.1         38-522
            534-833             AK087555.1         524-823
            834-1476            AK148371.1         823-1465
            1477-1488           AK087555.1         1467-1478
            1489-2012           AK148371.1         1478-2001
            2013-2032           CD776821.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 44.9 cM"
     gene            1..2032
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /note="milk fat globule EGF and factor V/VIII domain
                     containing"
                     /db_xref="GeneID:17304"
                     /db_xref="MGI:MGI:102768"
     exon            1..166
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            167..289
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            290..421
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            422..603
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            604..756
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            757..901
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1086
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1236
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     exon            1237..2015
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1992..1997
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /note="hexamer: AATACA"
     polyA_site      2015
                     /gene="Mfge8"
                     /gene_synonym="lactadherin; MFG-E8; Mfgm; MP47; P47; SED1"
                     /note="major polyA site"
ORIGIN      
        1 ggcgcctgat ttattccgga gtgagaggag cggacgcagg aactctcgag tcccagcatc
       61 agagcgcgtg gaccttttcc cgcgtcccgc agcatgcagg tctcccgtgt gctggccgcg
      121 ctgtgcggca tgctactctg cgcctctggc ctcttcgccg cgtctggtga cttctgtgac
      181 tccagcctgt gcctgaacgg tggcacctgc ttgacgggcc aagacaatga catctactgc
      241 ctctgccctg aaggcttcac aggccttgtg tgcaatgaga ctgagagagg accatgctcc
      301 ccaaaccctt gctacaatga tgccaaatgt ctggtgactt tggacacaca gcgtggggac
      361 atcttcaccg aatacatctg ccagtgccct gtgggctact cgggcatcca ctgtgaaacc
      421 ggttgttcta cacagctggg catggaaggg ggcgccattg ctgattcaca gatttccgcc
      481 tcgtctgtgt atatgggttt catgggcttg cagcgctggg gcccggagct ggctcgtctg
      541 taccgcacag ggatcgtcaa tgcctggaca gccagcaact atgatagcaa gccctggatc
      601 caggtgaacc ttctgcggaa gatgcgggta tcaggtgtga tgacgcaggg tgccagccgt
      661 gccgggaggg cggagtacct gaagaccttc aaggtggctt acagcctcga cggacgcaag
      721 tttgagttca tccaggatga aagcggtgga gacaaggagt ttttgggtaa cctggacaac
      781 aacagcctga aggttaacat gttcaacccg actctggagg cacagtacat aaagctgtac
      841 cctgtttcgt gccaccgcgg ctgcaccctc cgcttcgagc tcctgggctg tgagttgcac
      901 ggatgttctg agcccctggg cctgaagaat aacacaattc ctgacagcca gatgtcagcc
      961 tccagcagct acaagacatg gaacctgcgt gcttttggct ggtaccccca cttgggaagg
     1021 ctggataatc agggcaagat caatgcctgg acggctcaga gcaacagtgc caaggaatgg
     1081 ctgcaggttg acctgggcac tcagaggcaa gtgacaggaa tcatcaccca gggggcccgt
     1141 gactttggcc acatccagta tgtggcgtcc tacaaggtag cccacagtga tgatggtgtg
     1201 cagtggactg tatatgagga gcaaggaagc agcaaggtct tccagggcaa cttggacaac
     1261 aactcccaca agaagaacat cttcgagaaa cccttcatgg ctcgctacgt gcgtgtcctt
     1321 ccagtgtcct ggcataaccg catcaccctg cgcctggagc tgctgggctg ttaatgctca
     1381 gtcctgccag cccaaacgat gaggatggcc agaggctgag gggcctcctg gccctgcctc
     1441 ccaggccctg ctgccttctg tggctgacga ccttcttggc cttcccttct gattgtactg
     1501 gggctggagg caggaagggc caggggattt cagagttgcc cttcaccctt tccctcaccc
     1561 tgcagccccc acaggcctcc tgctagcccc cttctctcag gcattctggg ggagttggac
     1621 aggtctgaga tgaatagaga agaagagtga agttggggta tgtgggctat ctgtaccaac
     1681 caccccaagt cctaaacttc ctgccagggc ttgactcagg actgaaggga gcccctgact
     1741 gcccatccct ctctgcacac cacacattcc tccatgttcc attccgggaa ggagaggccc
     1801 acgtccgctt gctgtccctt gggtcaccag gtcctgcctc ttatctcctg agacgcctct
     1861 tgacccttgc actggagcct cagttgacaa ggagactggc gggtctggag aggtcggtgg
     1921 ctctgggtgg ttgacaggtt ggctgtggga cctctgctgg cttgctaccc aagttaacaa
     1981 gcagattcca aaatacattc gtgttctcca ctggaaaaaa aaaaaaaaaa aa
//
