LOCUS       NM_001310695            1752 bp    mRNA    linear   ROD 07-JUL-2024
DEFINITION  Mus musculus mitochondrial fission factor (Mff), transcript variant
            2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001310695 XM_006496570
VERSION     NM_001310695.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Takeichi,Y., Miyazawa,T., Sakamoto,S., Hanada,Y., Wang,L.,
            Gotoh,K., Uchida,K., Katsuhara,S., Sakamoto,R., Ishihara,T.,
            Masuda,K., Ishihara,N., Nomura,M. and Ogawa,Y.
  TITLE     Non-alcoholic fatty liver disease in mice with hepatocyte-specific
            deletion of mitochondrial fission factor
  JOURNAL   Diabetologia 64 (9), 2092-2107 (2021)
   PUBMED   34052855
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Varuzhanyan,G., Chen,H., Rojansky,R., Ladinsky,M.S., McCaffery,J.M.
            and Chan,D.C.
  TITLE     Mitochondrial fission factor (Mff) is required for organization of
            the mitochondrial sheath in spermatids
  JOURNAL   Biochim Biophys Acta Gen Subj 1865 (5), 129845 (2021)
   PUBMED   33476744
  REMARK    GeneRIF: Mitochondrial fission factor (Mff) is required for
            organization of the mitochondrial sheath in spermatids.
REFERENCE   3  (bases 1 to 1752)
  AUTHORS   Hanada,Y., Ishihara,N., Wang,L., Otera,H., Ishihara,T., Koshiba,T.,
            Mihara,K., Ogawa,Y. and Nomura,M.
  TITLE     MAVS is energized by Mff which senses mitochondrial metabolism via
            AMPK for acute antiviral immunity
  JOURNAL   Nat Commun 11 (1), 5711 (2020)
   PUBMED   33177519
  REMARK    GeneRIF: MAVS is energized by Mff which senses mitochondrial
            metabolism via AMPK for acute antiviral immunity.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1752)
  AUTHORS   Zhao,Y., Sun,X., Hu,D., Prosdocimo,D.A., Hoppel,C., Jain,M.K.,
            Ramachandran,R. and Qi,X.
  TITLE     ATAD3A oligomerization causes neurodegeneration by coupling
            mitochondrial fragmentation and bioenergetics defects
  JOURNAL   Nat Commun 10 (1), 1371 (2019)
   PUBMED   30914652
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1752)
  AUTHORS   Lewis,T.L. Jr., Kwon,S.K., Lee,A., Shaw,R. and Polleux,F.
  TITLE     MFF-dependent mitochondrial fission regulates presynaptic release
            and axon branching by limiting axonal mitochondria size
  JOURNAL   Nat Commun 9 (1), 5008 (2018)
   PUBMED   30479337
  REMARK    GeneRIF: the Drp1 'receptor' Mitochondrial fission factor (MFF) is
            required for determining the size of mitochondria entering the
            axon.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1752)
  AUTHORS   Long,B., Wang,K., Li,N., Murtaza,I., Xiao,J.Y., Fan,Y.Y., Liu,C.Y.,
            Li,W.H., Cheng,Z. and Li,P.
  TITLE     miR-761 regulates the mitochondrial network by targeting
            mitochondrial fission factor
  JOURNAL   Free Radic Biol Med 65, 371-379 (2013)
   PUBMED   23867156
  REMARK    GeneRIF: This study reveals a novel model of mitochondrial fission
            regulation, which is composed of miR-761 and mitochondrial fission
            factor
REFERENCE   7  (bases 1 to 1752)
  AUTHORS   Palmer,C.S., Elgass,K.D., Parton,R.G., Osellame,L.D.,
            Stojanovski,D. and Ryan,M.T.
  TITLE     Adaptor proteins MiD49 and MiD51 can act independently of Mff and
            Fis1 in Drp1 recruitment and are specific for mitochondrial fission
  JOURNAL   J Biol Chem 288 (38), 27584-27593 (2013)
   PUBMED   23921378
  REMARK    GeneRIF: MiD49 and MiD51 can act independently of Mff and Fis1 in
            Drp1 recruitment and suggest that they provide specificity to the
            division of mitochondria.
REFERENCE   8  (bases 1 to 1752)
  AUTHORS   Loson,O.C., Song,Z., Chen,H. and Chan,D.C.
  TITLE     Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in
            mitochondrial fission
  JOURNAL   Mol Biol Cell 24 (5), 659-667 (2013)
   PUBMED   23283981
  REMARK    GeneRIF: immunofluorescence analysis of Drp1 suggests that Fis1 and
            Mff are important for the number and size of Drp1 puncta on
            mitochondria.
REFERENCE   9  (bases 1 to 1752)
  AUTHORS   Otera,H., Wang,C., Cleland,M.M., Setoguchi,K., Yokota,S.,
            Youle,R.J. and Mihara,K.
  TITLE     Mff is an essential factor for mitochondrial recruitment of Drp1
            during mitochondrial fission in mammalian cells
  JOURNAL   J Cell Biol 191 (6), 1141-1158 (2010)
   PUBMED   21149567
REFERENCE   10 (bases 1 to 1752)
  AUTHORS   Dixit,E., Boulant,S., Zhang,Y., Lee,A.S., Odendall,C., Shum,B.,
            Hacohen,N., Chen,Z.J., Whelan,S.P., Fransen,M., Nibert,M.L.,
            Superti-Furga,G. and Kagan,J.C.
  TITLE     Peroxisomes are signaling platforms for antiviral innate immunity
  JOURNAL   Cell 141 (4), 668-681 (2010)
   PUBMED   20451243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AU080386.1, AK017226.1 and BC059229.1.
            
            On Jul 17, 2015 this sequence version replaced XM_006496570.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice junction and lacks an alternate in-frame exon compared to
            variant 1. The resulting isoform (2) has the same N- and C-termini
            but is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017226.1, BY716952.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AU080386.1         2-30
            30-1698             AK017226.1         2-1670
            1699-1752           BC059229.1         1827-1880
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.45 cM"
     gene            1..1752
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /note="mitochondrial fission factor"
                     /db_xref="GeneID:75734"
                     /db_xref="MGI:MGI:1922984"
     exon            1..195
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            196..304
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            305..525
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             345..1064
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="mitochondrial fission factor isoform 2"
                     /protein_id="NP_001297624.1"
                     /db_xref="CCDS:CCDS78630.1"
                     /db_xref="GeneID:75734"
                     /db_xref="MGI:MGI:1922984"
                     /translation="MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQEFQDGV
                     PNASVIMQVPERIVVTGNNEDISFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLD
                     FLDLERPLPTPQSEEQSRAVGRLKRERSMSENAVRQNGQLVRNDSIPVLRGGSAAATS
                     NPHHDNVRYGISNLDAAIEGASDDMTVVDAASLRRQIIKLNRRLQLLEEENKERAKRE
                     MVMYSITVAFWLLNSWLWFRR"
     exon            526..695
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            696..787
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            788..847
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            848..932
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1738
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1721..1726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1738
                     /gene="Mff"
                     /gene_synonym="5230400G24Rik"
                     /note="major polyA site"
ORIGIN      
        1 acttccgcta cggctcctgg aaggggtcag tcctggccgt tctcgcgtct gtcccggccc
       61 gtcgcggccg ctccgccgct tctttgcccg ggcgcctcgg tgcgctcggt caacggcctc
      121 tgcgagcgct cacccgagtg ccccggagcc ggcgggcggc gaccctctct ccggggactc
      181 tccagcaggc ccaggggata aaagtggctc tcaccctagc acatgtgcat cggagcaatt
      241 taaggagtta atatgaagca cagaagcaga tagtgccaaa tagcaagcct gttgtttcac
      301 atttggtgag tggggcagca tttccttctc ccactgctgc tgagatggca gaaattagtc
      361 gaattcagta tgaaatggaa tacaccgaag gtattagtca gcgaatgagg gtcccggaaa
      421 aattaaaagt agcaccacca aatgctgacc tggagcaaga gttccaagat ggagttccaa
      481 atgccagtgt gataatgcaa gtcccagaga ggatcgtggt tacaggaaat aatgaagaca
      541 tttcattttc aagaccagca gatcttgacc ttatccagtc cactcccttt aaacctctgg
      601 cactgaaaac accaccacgc gtgctcacac tgagtgagag accactagat ttcctggatt
      661 tagaaagacc acttccaact cctcaaagcg aagagcagag ccgagcagtt ggcaggctaa
      721 aaagagagcg ttctatgagt gaaaatgctg ttcgccaaaa tggacagttg gtcagaaacg
      781 attccatacc tgtgttgcgt ggtgggtcag ctgccgccac ttctaatcct catcatgaca
      841 acgtcaggta tggcatttca aacctagatg cagccattga aggagcttca gatgacatga
      901 ctgtggtaga tgcagcttca ttaagacgtc agataatcaa attaaataga cgtctccaac
      961 ttctggaaga ggagaataaa gagcgtgcta aaagagaaat ggtcatgtat tcaatcactg
     1021 tagcgttctg gctgcttaac agctggctct ggtttcgacg ctagaggtaa cctcagcatt
     1081 cacatatatt gtctcaacac ctggaaatat aaaggatttg caaacttcat tgtttctgtc
     1141 tttgcatcat cgtatgacgg ccctgaaaat gtatttctcc agagtgcagg gaaggccctg
     1201 tatctgtaga gtgaaggcct gtggtgctgc tgatgggcgt tcagccagtt gtgcagtggc
     1261 acctatacac tctccttttg catgtcttgt aaataggctc taggtttgtt ttgtttttaa
     1321 agaaaataat ttttgtggcc tcaccagtct gtacagctaa ttctctaaac agaaatggca
     1381 gtcataaaga gtgagtttag aaaactgtct gtgaaagaaa agtgttttgt cctgttttca
     1441 gatgatgaag cgatttcttt gatagatgct ttttgtatct acatctcaac tgtaatgctt
     1501 tctaagtcac agtgctgtgc aggactgaag tcaaaccacc tgactctgct tcatcgccta
     1561 cctgcacagg cggggctaaa aactcctaac agttcagcag tctgtctgtg tgcaatgctg
     1621 ttttgacttt catcttagtt ttctatcaag tatgttttta ccaagtccat gtgtgaatga
     1681 tttaaaaacc taaaaaataa ggtataaatg tagtgtattt aataaactgt caaccaaaaa
     1741 aaaaaaaaaa aa
//
