LOCUS       NM_001161799            1044 bp    mRNA    linear   ROD 13-MAY-2023
DEFINITION  Mus musculus microfibrillar-associated protein 2 (Mfap2),
            transcript variant 2, mRNA.
ACCESSION   NM_001161799
VERSION     NM_001161799.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Xue M, Mi S, Zhang Z, Wang H, Chen W, Wei W and Lou G.
  TITLE     MFAP2, upregulated by m1A methylation, promotes colorectal cancer
            invasiveness via CLK3
  JOURNAL   Cancer Med 12 (7), 8403-8414 (2023)
   PUBMED   36583532
  REMARK    GeneRIF: MFAP2, upregulated by m1A methylation, promotes colorectal
            cancer invasiveness via CLK3.
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Mead TJ, Martin DR, Wang LW, Cain SA, Gulec C, Cahill E, Mauch J,
            Reinhardt D, Lo C, Baldock C and Apte SS.
  TITLE     Proteolysis of fibrillin-2 microfibrils is essential for normal
            skeletal development
  JOURNAL   Elife 11, e71142 (2022)
   PUBMED   35503090
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1044)
  AUTHORS   Menko AS, DeDreu J, Logan CM, Paulson H, Levin AV and Walker JL.
  TITLE     Resident immune cells of the avascular lens: Mediators of the
            injury and fibrotic response of the lens
  JOURNAL   FASEB J 35 (4), e21341 (2021)
   PUBMED   33710665
REFERENCE   4  (bases 1 to 1044)
  AUTHORS   Chen Z, Lv Y, Cao D, Li X and Li Y.
  TITLE     Microfibril-Associated Protein 2 (MFAP2) Potentiates Invasion and
            Migration of Melanoma by EMT and Wnt/beta-Catenin Pathway
  JOURNAL   Med Sci Monit 26, e923808 (2020)
   PUBMED   32464633
  REMARK    GeneRIF: Microfibril-Associated Protein 2 (MFAP2) Potentiates
            Invasion and Migration of Melanoma by EMT and Wnt/beta-Catenin
            Pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1044)
  AUTHORS   Li J, Deng SP, Wei G and Yu P.
  TITLE     CITGeneDB: a comprehensive database of human and mouse genes
            enhancing or suppressing cold-induced thermogenesis validated by
            perturbation experiments in mice
  JOURNAL   Database (Oxford) 2018 (2018)
   PUBMED   29688375
REFERENCE   6  (bases 1 to 1044)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   7  (bases 1 to 1044)
  AUTHORS   Segade F, Broekelmann TJ, Pierce RA and Mecham RP.
  TITLE     Revised genomic structure of the human MAGP1 gene and
            identification of alternate transcripts in human and mouse tissues
  JOURNAL   Matrix Biol 19 (7), 671-682 (2000)
   PUBMED   11102756
REFERENCE   8  (bases 1 to 1044)
  AUTHORS   Faraco J, Bashir M, Rosenbloom J and Francke U.
  TITLE     Characterization of the human gene for microfibril-associated
            glycoprotein (MFAP2), assignment to chromosome 1p36.1-p35, and
            linkage to D1S170
  JOURNAL   Genomics 25 (3), 630-637 (1995)
   PUBMED   7759096
REFERENCE   9  (bases 1 to 1044)
  AUTHORS   Davis EC.
  TITLE     Immunolocalization of microfibril and microfibril-associated
            proteins in the subendothelial matrix of the developing mouse aorta
  JOURNAL   J Cell Sci 107 (Pt 3), 727-736 (1994)
   PUBMED   8006086
REFERENCE   10 (bases 1 to 1044)
  AUTHORS   Chen Y, Faraco J, Yin W, Germiller J, Francke U and Bonadio J.
  TITLE     Structure, chromosomal localization, and expression pattern of the
            murine Magp gene
  JOURNAL   J Biol Chem 268 (36), 27381-27389 (1993)
   PUBMED   8262979
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645625.15.
            
            [WARNING] On Jun 14, 2023 this sequence was replaced by
            NM_001161799.3.
            
            On Apr 1, 2019 this sequence version replaced NM_001161799.1.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus. The use of an alternative upstream start codon, which
            appears to be rodent-specific, would result in a protein that is 2
            aa longer at the N-terminal.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DV654229.1, CO812500.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AL645625.15        93188-93246
            60-135              AL645625.15        95590-95665
            136-222             AL645625.15        96537-96623
            223-249             AL645625.15        96820-96846
            250-330             AL645625.15        96953-97033
            331-384             AL645625.15        97159-97212
            385-472             AL645625.15        97747-97834
            473-546             AL645625.15        97979-98052
            547-1044            AL645625.15        98241-98738
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4"
     gene            1..1044
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /note="microfibrillar-associated protein 2"
                     /db_xref="GeneID:17150"
                     /db_xref="MGI:MGI:99559"
     exon            1..59
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            60..135
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..650
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /note="isoform a precursor is encoded by transcript
                     variant 2; microfibril-associated glycoprotein 1; MAGP-1;
                     MFAP-2; microfibril-associated glycoprotein 1A;
                     microfibril-associated glycoprotein 1D"
                     /codon_start=1
                     /product="microfibrillar-associated protein 2 isoform a
                     precursor"
                     /protein_id="NP_001155271.2"
                     /db_xref="CCDS:CCDS18860.2"
                     /db_xref="GeneID:17150"
                     /db_xref="MGI:MGI:99559"
                     /translation="MRAACLFLLFMPGLLAQGQYDLDPLPPFPDHVQYNHYGDQIDNA
                     DYYDYQEVSPRTPEEQFQSQQQVQQEVIPAPTPEPAAAGDLETEPTEPGPLDCREEQY
                     PCTRLYSIHKPCKQCLNEVCFYSLRRVYVVNKEICVRTVCAHEELLRADLCRDKFSKC
                     GVMAVSGLCQSVAASCARSCGGC"
     exon            136..222
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            223..249
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            250..330
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            331..384
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            385..472
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..546
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
     exon            547..1044
                     /gene="Mfap2"
                     /gene_synonym="Magp; Magp1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggagaaagca agcaaggttt catttccagc cccgtgacct ctgggctctg ggtggagagc
       61 tgtcctcaac gccaccaccg cctcctcact ccatggccat gagagccgcc tgcctcttcc
      121 tgctgttcat gcctggcctg ctggctcagg gccaatatga cctggatcct ctccccccat
      181 tcccggacca tgtccagtac aaccactatg gcgaccagat tgacaacgca gactactatg
      241 actaccaaga agtgagtcct cggacccctg aagagcagtt ccagtcccag cagcaagttc
      301 aacaggaagt catcccagcc cctaccccag agccagcagc tgcaggggac ctggagactg
      361 agcctaccga gcctggccct cttgactgcc gcgaagaaca gtacccgtgc acccgcctct
      421 actccatcca caagccttgc aaacagtgtc tcaatgaggt ctgcttctac agcctccgcc
      481 gagtgtatgt ggtcaacaag gaaatatgtg tccgcactgt ctgtgcccac gaggaacttc
      541 tccgagctga cctgtgccga gacaagttct ccaagtgtgg cgtgatggcc gtcagtggcc
      601 tgtgccaatc tgtggctgcc tcctgtgcca ggagctgtgg gggctgctag ggtggagctg
      661 gtgtgagatt cgagccctgg acccagctcg gatccacctc aggccctgcc tgacctggtg
      721 ctttctcccc atcctgccct ccctttcatc ccggacatca gtgggctgct accctgggga
      781 gtgtggtcac agctgccttg ggcccctccc catccttaag tgggggctcc taactgtcca
      841 gtctccaccc cttagtgggg ttcatccaag gcctcgttgt ggggcctggg cttctcactg
      901 tgccttctca gtgcctgggg gcagccctcc tcagtctcct caggtaggct gtgcccctta
      961 cccagctggt ctgcttggat ttcctacgcc cccaggcatg gaccacctct gttttataca
     1021 aaataataaa agtttttaca aaag
//
