LOCUS       NM_145524               2348 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus methyltransferase 8, methylcytidine (Mettl8),
            transcript variant a, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_145524
VERSION     NM_145524.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Zhang,F., Yoon,K., Zhang,D.Y., Kim,N.S., Ming,G.L. and Song,H.
  TITLE     Epitranscriptomic regulation of cortical neurogenesis via
            Mettl8-dependent mitochondrial tRNA m3C modification
  JOURNAL   Cell Stem Cell 30 (3), 300-311 (2023)
   PUBMED   36764294
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Li,X., Bi,H., Xie,S. and Cui,W.
  TITLE     MiR-208b Regulates the Conversion of Skeletal Muscle Fiber Types by
            Inhibiting Mettl8 Expression
  JOURNAL   Front Genet 13, 820464 (2022)
   PUBMED   35281804
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2348)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2348)
  AUTHORS   Gu,H., Do,D.V., Liu,X., Xu,L., Su,Y., Nah,J.M., Wong,Y., Li,Y.,
            Sheng,N., Tilaye,G.A., Yang,H., Guo,H., Yan,J. and Fu,X.Y.
  TITLE     The STAT3 Target Mettl8 Regulates Mouse ESC Differentiation via
            Inhibiting the JNK Pathway
  JOURNAL   Stem Cell Reports 10 (6), 1807-1820 (2018)
   PUBMED   29706498
  REMARK    GeneRIF: METTL8 prohibits the activation of JNK signaling and
            enhances the differentiation of mouse ESCs. Collectively, our study
            uncovers a STAT3 target, Mettl8, which regulates mouse ESC
            differentiation via JNK signaling.
REFERENCE   5  (bases 1 to 2348)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
REFERENCE   6  (bases 1 to 2348)
  AUTHORS   Badri,K.R., Zhou,Y., Dhru,U., Aramgam,S. and Schuger,L.
  TITLE     Effects of the SANT domain of tension-induced/inhibited proteins
            (TIPs), novel partners of the histone acetyltransferase p300, on
            p300 activity and TIP-6-induced adipogenesis
  JOURNAL   Mol Cell Biol 28 (20), 6358-6372 (2008)
   PUBMED   18710950
  REMARK    GeneRIF: TIP-6 was identified as a new member of the adipogenic
            cascade.
REFERENCE   7  (bases 1 to 2348)
  AUTHORS   Jakkaraju,S., Zhe,X., Pan,D., Choudhury,R. and Schuger,L.
  TITLE     TIPs are tension-responsive proteins involved in myogenic versus
            adipogenic differentiation
  JOURNAL   Dev Cell 9 (1), 39-49 (2005)
   PUBMED   15992539
REFERENCE   8  (bases 1 to 2348)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929228.5.
            
            On Jan 10, 2022 this sequence version replaced NM_145524.3.
            
            Summary: This locus encodes a member of the methyltransferase
            family, and is involved in chromatin remodeling. Transcripts from
            this locus can be induced or inhibited by cell stretch and affect
            cell differentiation in the myogenic or adipogenic pathways.
            Multiple transcript variants encoding different isoforms have been
            found for this gene. Additional splice variants have been described
            in the literature but they meet nonsense-mediated decay (NMD)
            criteria and are likely to be degraded as soon as they are
            transcribed. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (a), also known as TIP-4,
            represents the longer transcript and encodes the longer isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ093358.1, SRR13422598.206512.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-214               AL929228.5         99993-100206        c
            215-369             AL929228.5         62842-62996         c
            370-461             AL929228.5         38106-38197         c
            462-802             AL929228.5         26477-26817         c
            803-852             AL929228.5         24999-25048         c
            853-916             AL929228.5         20696-20759         c
            917-1056            AL929228.5         17875-18014         c
            1057-1163           AL929228.5         11672-11778         c
            1164-1229           AL929228.5         11070-11135         c
            1230-2348           AL929228.5         9183-10301          c
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 42.34 cM"
     gene            1..2348
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /note="methyltransferase 8, methylcytidine"
                     /db_xref="GeneID:228019"
                     /db_xref="MGI:MGI:2385142"
     exon            1..214
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            215..369
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            370..461
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            462..802
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            803..852
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            853..916
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1056
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1163
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1229
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..2348
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2328..2333
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /note="hexamer: AATAAA"
     polyA_site      2348
                     /gene="Mettl8"
                     /gene_synonym="Tip"
                     /note="major polyA site"
ORIGIN      
        1 agtgggtcgg gtagccttcc gggctgcacg cggtaaacct ggatagcttt ggtgggtgct
       61 gtgcagccac ctggtggtgt cgcttcacct gtgtgtccca ggaagcagtc cctttctgcc
      121 ctggtcgtgg gacgactgcc aacccctcct ttggccctgc aaggctctgg ccacgcgtcc
      181 agaggggcgt tgtggccagt gggaccctcc aaaggtcctg cttaggatga atgtgatttg
      241 gagaagttgc atttgccgac tgaggcaggg gaaggtacca caccgatgcc agagtggcgt
      301 ccacccggta gcccctctgg ggtcgaggat tttaactgac cccgccaaag tttttgaaca
      361 caacatgtgg gatcacatgc agtggtctaa agaagaagaa gacgcagcta gaaaaaaggt
      421 agaggaaaac tcagccacac gagtcgctcc agaagagcaa gttaagtttg aaagtgacgc
      481 taacaaatac tgggatatat tttaccagac ccataagaat aagtttttca agaatcgtaa
      541 ttggctgttg agggaatttc ctgaaatcct tcctgttaat caaaacacta aagagaaagt
      601 gggagaatca tcctgggatc aagtcggaag cagcatctct aggacacaag gaacagaaac
      661 ccattgtcaa gagagttttg tctcgccaga acctgggagc agaggacggt ctgctcctga
      721 ccccgacttg gaagaataca gcaaaggacc tgggaagaca gagccgtttc ctggtagcaa
      781 tgccactttt cgaatactag aggttggctg tggtgctgga aatagcgtgt ttccaatcct
      841 gaacaccttg cagaacattc caggatcctt tctctactgc tgcgactttg cctctgaagc
      901 tgtggaactt gtaaagtccc acgagtccta cagcgaggcc cagtgttctg cctttattca
      961 tgacgtgtgt gacgacggct tagcctaccc tttcccagat gggatcctgg atgtcgttct
     1021 ccttgtcttt gtgctctcat ctatccaccc tgacaggatg caagctgttg cccaccgact
     1081 gtccaggttg ctgaagcccg gaggaatgct attgtttcgg gatcatggaa gatacgataa
     1141 tgctcagctt cgttttaaga aagggcgttg tttatctgaa aatttttatg tccgaggaga
     1201 tggtaccaga gcttatttct ttacaaaagg ggaaatccgc cgtatgttct gcgaggctgg
     1261 attacacgaa aagcaaaatc tggttgatca tcgcttgcaa gtgaacagga aaaagcaagt
     1321 gcagatgcac cgagtgtgga ttcaaggaaa attccagaaa ccatcgccct ggactccaca
     1381 gagtggaaat tagcattcac tgcattctca ggaccgaact atgtgtcatg ttaaagtatg
     1441 aacccagggc ctgatactgt tcaaagactt ctcttccaga gatgagcaga agagcatccg
     1501 cagctctgct ggtctgctgt cagtgctctc aggacatttt aagaacaagg atgtgacttg
     1561 aaagagtctc ccaccctctg ggtttccaga acttgaaatg tatgtttggt tttgtttcat
     1621 ctaaccatgt tcccttgaat tgtatgactt tgaattaaga agcgtatagt tttgagaaat
     1681 gtaacccgtc catgttttag aagtggaaaa atgatctact ttattgttga gtatggtaaa
     1741 gcagtcaaac tgccctctgc tgtcagccag tagcagcggc atagtgtgcc ttgttcttgg
     1801 gggaactcag gatgaagggt tactgtgttt tgttctgttc ttatctattt tctctcaaaa
     1861 ctcaccaacc accaccacca ccaccaccac acacaagtgt ctgaaataca accttagttt
     1921 ctctttccaa aaaatgggga agtgtgaccc ttgttaacat cacttccggg ctattcttag
     1981 atatgcagat catctttgag acactgtctg tgctgtggtg gccagtttta attgtcaact
     2041 tgacacactc tagaaccacc caggaagagt ctcagattgt cagttgtctg tgagattgtc
     2101 ttgattgtat taattgatgt gggaagactt gtctactgtg ggcagtacca ttccctggag
     2161 gttggaccct gaaccaagta agtgtgggaa aagggagctg agcactggca caccagtatt
     2221 catgctcgct cttctcctga ccatgggtgt aaccagctgc ttcaagctcc tgcctccttg
     2281 acttcctgct gtaactgggt ggactgtagc ttggagttgt gagctaaaat aaaccttttc
     2341 ttccaaaa
//
