LOCUS       NM_025907               1968 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus methyltransferase 6, methylcytidine (Mettl6),
            transcript variant 1, mRNA.
ACCESSION   NM_025907
VERSION     NM_025907.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Ignatova,V.V., Kaiser,S., Ho,J.S.Y., Bing,X., Stolz,P., Tan,Y.X.,
            Lee,C.L., Gay,F.P.H., Lastres,P.R., Gerlini,R., Rathkolb,B.,
            Aguilar-Pimentel,A., Sanz-Moreno,A., Klein-Rodewald,T.,
            Calzada-Wack,J., Ibragimov,E., Valenta,M., Lukauskas,S., Pavesi,A.,
            Marschall,S., Leuchtenberger,S., Fuchs,H., Gailus-Durner,V., de
            Angelis,M.H., Bultmann,S., Rando,O.J., Guccione,E., Kellner,S.M.
            and Schneider,R.
  TITLE     METTL6 is a tRNA m3C methyltransferase that regulates pluripotency
            and tumor cell growth
  JOURNAL   Sci Adv 6 (35), eaaz4551 (2020)
   PUBMED   32923617
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1968)
  AUTHORS   Xu,L., Liu,X., Sheng,N., Oo,K.S., Liang,J., Chionh,Y.H., Xu,J.,
            Ye,F., Gao,Y.G., Dedon,P.C. and Fu,X.Y.
  TITLE     Three distinct 3-methylcytidine (m3C) methyltransferases modify
            tRNA and mRNA in mice and humans
  JOURNAL   J Biol Chem 292 (35), 14695-14703 (2017)
   PUBMED   28655767
REFERENCE   4  (bases 1 to 1968)
  AUTHORS   Sampath Kumar,A., Seah,M.K., Ling,K.Y., Wang,Y., Tan,J.H.,
            Nitsch,S., Lim,S.L., Lorthongpanich,C., Wollmann,H., Low,D.H.,
            Guccione,E. and Messerschmidt,D.M.
  TITLE     Loss of maternal Trim28 causes male-predominant early embryonic
            lethality
  JOURNAL   Genes Dev 31 (1), 12-17 (2017)
   PUBMED   28115466
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154616.2, AK005448.1 and AV241644.2.
            
            On Jan 18, 2018 this sequence version replaced NM_025907.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC030449.1, AK082949.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AC154616.2         153063-153072       c
            11-1755             AK005448.1         3-1747
            1756-1968           AV241644.2         427-639
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 19.12 cM"
     gene            1..1968
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /note="methyltransferase 6, methylcytidine"
                     /db_xref="GeneID:67011"
                     /db_xref="MGI:MGI:1914261"
     exon            1..139
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            140..507
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..1131
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /EC_number="2.1.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase-like protein 6; tRNA N(3)-methylcytidine
                     methyltransferase METTL6; methyltransferase like 6; tRNA
                     N(3)-cytidine methyltransferase METTL6"
                     /codon_start=1
                     /product="tRNA N(3)-cytidine methyltransferase METTL6
                     isoform 1"
                     /protein_id="NP_080183.1"
                     /db_xref="CCDS:CCDS26913.1"
                     /db_xref="GeneID:67011"
                     /db_xref="MGI:MGI:1914261"
                     /translation="MASFQRKGLQARILSTEEEEKLKRDQALVSAFKQQKLEKEAQKN
                     WDLFYKRNSTNFFKDRHWTTREFEELRSCREYEGQKLTLLEAGCGVGNCLFPLLEEDL
                     NLFAYACDFSPRAVDYVKQHPLYNAERCKVFQCDLTRDDLLDHVPPESVDAVTLIFVL
                     SAVHPEKMRLVLLNVYKVLKPGRSVLFRDYGLNDHAMLRFKAGSKLGENFYVRQDGTR
                     SYFFTDEFLAQLFVDAGYEEVVNEYVFRETVNKKEGLCVPRVFLQSKFRKPPKDPAPT
                     SDSASL"
     exon            508..642
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            643..813
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            814..955
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1968
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1945..1950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1968
                     /gene="Mettl6"
                     /gene_synonym="1600013P15Rik"
ORIGIN      
        1 tctccccaac ggaaatagcg ggggcagtgc catggagaga gagttctccg ggactgctgc
       61 agcccacagt tttctggtct aaggaggagc ccaggtcagc tagaagattc tgctgccgac
      121 gcctgggctt gactgacggc atcgtatcct gttggagctg gaaagtcacc tggggaaact
      181 gcatcagaga aactccagtg tctacaatct cagcatcgtt tgttcagatg gttgaagaac
      241 agaggaccta tagcagcatt gaatgtgttt ccggcaacta caatggcttc tttccaaagg
      301 aaaggactgc aagcaaggat tcttagcact gaagaagagg agaaactgaa aagagaccaa
      361 gctttggtgt ctgcttttaa acagcaaaaa ctggaaaaag aggctcagaa gaactgggac
      421 cttttttata aaagaaacag taccaatttt ttcaaggaca gacactggac caccagagag
      481 tttgaggagt tgagatcatg tagggagtat gaaggtcaaa aattgacttt gttggaggct
      541 ggctgtgggg ttgggaactg tttattccca cttttagaag aagacttgaa tctctttgcc
      601 tatgcctgtg atttttctcc aagggcagtt gactacgtga agcaacatcc tttgtacaat
      661 gcagagagat gcaaagtgtt ccagtgtgac ctcactagag acgacctgct tgaccacgtc
      721 ccaccagagt ctgtggatgc cgttacattg atctttgtgc tctcagctgt gcaccctgag
      781 aagatgcgcc ttgtcttact aaacgtgtac aaggtattaa aaccaggcag aagtgtctta
      841 ttccgtgact acgggctgaa tgatcacgcc atgcttagat ttaaagctgg aagcaaactt
      901 ggagaaaatt tttatgtcag gcaagatgga accagatcgt atttttttac tgatgaattc
      961 ctggcgcagc tctttgtgga tgcaggttat gaagaagtgg tgaacgagta tgtgtttcga
     1021 gagacagtga ataaaaaaga gggcctgtgt gtgcctcgag ttttccttca gagcaagttc
     1081 cggaagcctc cgaaggaccc agcccctacc agtgactctg cttcactttg aagaaaagga
     1141 tgttaaacta ttttgtattt tttttatatt ctgaattttt gaaaattgca tgtgttattt
     1201 ttgtactgag aacaagtgaa gactggggag ttggctcagc aggtaaaggt gctagctgtg
     1261 caagcttagc aacctgggtg gatcagcagt ctgtgttgag caggagagat ggctcagtga
     1321 tcaagagtgt tggctgctct tgggcagggc ctgggttcgg tttccagcac ccacatggca
     1381 gctcacttcc atcagtaatt ccagtcccag ggtattgagt gccctcttct gacctctgca
     1441 agtggtgcac agacatacat gcaggcaaac acccaaatta caaaagaaaa aaatccaggt
     1501 ttggcaattt gagtttatcc tagtactcct acctcagtac tcctacctca agatgagaag
     1561 cagagacgga agaaccatct ggaagcctca ggagcagcca gcctggagta cacggtgtgg
     1621 gagacctaag cctggagtac gcggtgtggg agacctagga gagaggcttc agagaggtgg
     1681 agagtgagag ccaatgagaa ttgtttttct gacctcacat gtgcacaaat aagtaaataa
     1741 agtggatttg tttgcacact gtagaaaaac agaattgtaa gtcctgaaag gaaagtcttg
     1801 tggcttagtt gtgttgtatg attatccatc tccacaaagg gaagcatggg agccgacaag
     1861 agatacatga acaggtcgca ggtcaagcat ctgctgatga tacatttaag acagttcttt
     1921 ggtgtgcata cagactgacc acttattaaa gactaaagca gacgtgca
//
