LOCUS       NM_001357135            7147 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus methyltransferase 4, N6-adenosine (Mettl4), transcript
            variant 1, mRNA.
ACCESSION   NM_001357135 XM_006525105
VERSION     NM_001357135.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Sang,A., Zhang,J., Zhang,M., Xu,D., Xuan,R., Wang,S., Song,X. and
            Li,X.
  TITLE     METTL4 mediated-N6-methyladenosine promotes acute lung injury by
            activating ferroptosis in alveolar epithelial cells
  JOURNAL   Free Radic Biol Med 213, 90-101 (2024)
   PUBMED   38224757
  REMARK    GeneRIF: METTL4 mediated-N6-methyladenosine promotes acute lung
            injury by activating ferroptosis in alveolar epithelial cells.
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Xiao,Z., Liu,S., Li,Z., Cui,J., Wang,H., Wang,Z., Ren,Q., Xia,L.,
            Wang,Z. and Li,Y.
  TITLE     The Maternal Microbiome Programs the m6A Epitranscriptome of the
            Mouse Fetal Brain and Intestine
  JOURNAL   Front Cell Dev Biol 10, 882994 (2022)
   PUBMED   35874829
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7147)
  AUTHORS   Wang,Y., Sun,J., Lin,Z., Zhang,W., Wang,S., Wang,W., Wang,Q. and
            Ning,G.
  TITLE     m6A mRNA Methylation Controls Functional Maturation in Neonatal
            Murine beta-Cells
  JOURNAL   Diabetes 69 (8), 1708-1722 (2020)
   PUBMED   32404350
REFERENCE   4  (bases 1 to 7147)
  AUTHORS   Zhang,Z., Hou,Y., Wang,Y., Gao,T., Ma,Z., Yang,Y., Zhang,P., Yi,F.,
            Zhan,J., Zhang,H. and Du,Q.
  TITLE     Regulation of Adipocyte Differentiation by METTL4, a 6 mA Methylase
  JOURNAL   Sci Rep 10 (1), 8285 (2020)
   PUBMED   32427889
  REMARK    GeneRIF: Regulation of Adipocyte Differentiation by METTL4, a 6 mA
            Methylase.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7147)
  AUTHORS   Kweon,S.M., Chen,Y., Moon,E., Kvederaviciute,K., Klimasauskas,S.
            and Feldman,D.E.
  TITLE     An Adversarial DNA N6-Methyladenine-Sensor Network Preserves
            Polycomb Silencing
  JOURNAL   Mol Cell 74 (6), 1138-1147 (2019)
   PUBMED   30982744
  REMARK    GeneRIF: It has been shown that 6methyladenine (6 mA) deposition
            and removal are catalyzed by the Mettl4 methyltransferase and
            Alkbh4 dioxygenase, respectively, and that 6mA accumulation in
            genic elements corresponds with transcriptional silencing.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165075.3 and BE633059.1.
            
            [WARNING] On Jul 31, 2024 this sequence was replaced by
            NM_001357135.2.
            
            On Oct 21, 2017 this sequence version replaced XM_006525105.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.151666.1,
                                           SRR10223970.19659.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-634               AC165075.3         132694-133327       c
            635-1435            AC165075.3         130925-131725       c
            1436-1498           AC165075.3         127240-127302       c
            1499-1868           AC165075.3         123694-124063       c
            1869-1938           AC165075.3         122762-122831       c
            1939-2113           AC165075.3         118644-118818       c
            2114-2220           AC165075.3         116855-116961       c
            2221-2312           AC165075.3         116397-116488       c
            2313-6781           AC165075.3         106235-110703       c
            6782-7147           BE633059.1         1-366
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 61.17 cM"
     gene            1..7147
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="methyltransferase 4, N6-adenosine"
                     /db_xref="GeneID:76781"
                     /db_xref="MGI:MGI:1924031"
     exon            1..634
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            635..1435
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     CDS             1043..2458
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /EC_number="2.1.1.72"
                     /note="isoform 1 is encoded by transcript variant 1;
                     methyltransferase-like protein 4; N(6)-adenine-specific
                     DNA methyltransferase METTL4; snRNA
                     (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4;
                     N(6)-adenine-specific methyltransferase METTL4;
                     methyltransferase like 4"
                     /codon_start=1
                     /product="N(6)-adenine-specific methyltransferase METTL4
                     isoform 1"
                     /protein_id="NP_001344064.1"
                     /db_xref="CCDS:CCDS29030.1"
                     /db_xref="GeneID:76781"
                     /db_xref="MGI:MGI:1924031"
                     /translation="MSVVHHLPPGWLLDHLSFINKVNYQLCQHQESFCSKNNPTSSVY
                     MDSLQLDPGSPFGAPAMCFAPDFTTVSGNDDEGSCEVITEKYVFRSELFNVTKPYIVP
                     AVHKERQQSNKNENLVTDYKQEVSVSVGKKRKRCIAFNQGELDAMEYHTKIRELILDG
                     SSKLIQEGLRSGFLYPLVEKQDGSSGCITLPLDACNLSELCEMAKHLPSLNEMELQTL
                     QLMGDDVSVIELDLSSQIIENNSSFSKMITLMGQKYLLPPQSSFLLSDISCMQPLLNC
                     GKTFDAIVIDPPWENKSVKRSNRYSSLSPQQIKRMPIPKLAAADCLIVTWVTNRQKHL
                     CFVKEELYPSWSVEVVAEWYWVKITNSGEFVFPLDSPHKKPYECLVLGRVKEKTPLAL
                     RNPDVRIPPVPDQKLIVSVPCVLHSHKPPLTEVLRDYIKPGGQCLELFARNLQPGWMS
                     WGNEVLKFQHMDYFIALESGC"
     exon            1436..1498
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1868
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..1938
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2113
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2220
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2312
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..7118
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3736..3741
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="hexamer: AATAAA"
     polyA_site      3755
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
     regulatory      7030..7035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="hexamer: AATAAA"
     regulatory      7041..7046
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="hexamer: ATTAAA"
     polyA_site      7050
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="major polyA site"
     polyA_site      7058
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
     regulatory      7092..7097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
                     /note="hexamer: AATAAA"
     polyA_site      7118
                     /gene="Mettl4"
                     /gene_synonym="2410198H06Rik; A730091E08Rik; HsT661"
ORIGIN      
        1 gcatcggcgg ctcaacgcgc tccgggctcc tcccaggtca tcgtcggtgt cggcgacctg
       61 aagggagttc catcctatcc gggaagctgg cgtctaggac gagtagtgct gtcgggcgga
      121 gcggcccgag ccctcctgga gtcgagttcc gtacagcaat atggccgtgt cctggaacta
      181 gaaagcgagg gtttgtttcc gaaaggatgg aggccaggac ctgttttaga tgagtgttgt
      241 ttccacgtgg cgagcctggg aggcggcgtg acgtttcctg acggtaggag aggctgtcga
      301 gagtggcggc ggaagagcct gagggatatt tatcgtcagg ctgcctcaag tgctgtggac
      361 taccgccgcc cagggtcgga actacaagca ggcgttgcca gttcccgcac ggtggtggcc
      421 tgtccctggg ctaaagtgta gatgattccg gggtctcagc gcaattgtgc cattagctag
      481 ttttgagcta agcatttttt tttttttttt tgagagtttg aggttcccta gcagtcacag
      541 tgtctcctca catcacgtct cgactgggaa accaaaatct caggtatcac gaagatctcc
      601 attaccaaat agcagtcagc agatatatta gaaggacact caccttttaa ggatttagtg
      661 acttgaatac ttggaccatg tggactcgtg ccttgattcg aggaccaaaa caggaccaca
      721 gaggacaccc gaagaaagga gaatcaaact aagaagcgtc tttgactaca ttggctgaaa
      781 attttgaatg accaattctg aaattaaaag acacttaaag tatcgcattt ttccttagag
      841 gtgatatgta cacactcacc ttgtatgttt aaatataaaa ccttcttgtg tgtacaaagt
      901 acatcgttga tatacaggac attaagaact taattacaac attggtatca acccaggacc
      961 cccatactga gctatactcc cagtcctagt tctgattctt gattatcagt tttttgtttt
     1021 tgttttttgt ttttttgtga gaatgtctgt ggtgcatcat ttgcctcctg ggtggttact
     1081 ggatcatctt tctttcatta acaaagtaaa ctatcaactt tgccagcatc aagagtcttt
     1141 ttgtagtaaa aacaacccta cttcttctgt ctacatggat tctcttcagc tggatcccgg
     1201 gtctcctttt ggagctcctg ctatgtgctt tgctcctgac ttcactactg tgtcgggcaa
     1261 tgacgatgaa ggaagttgtg aagtgatcac cgaaaaatat gttttccgat ctgagctatt
     1321 taatgtcacc aaaccttaca tagttccagc tgttcacaaa gagcgccaac aaagtaacaa
     1381 aaacgaaaat ctagtgactg actataaaca agaggtctct gtttctgttg ggaagaagcg
     1441 taaaagatgc attgctttca atcaaggtga attggatgcc atggagtatc atacaaagat
     1501 cagagagctg attttggatg gatcttcgaa gttaatccaa gaaggtctca gaagtggttt
     1561 tctttaccca cttgttgaaa aacaggatgg aagtagcgga tgcatcactt taccacttga
     1621 tgcctgcaat ttatcagagt tatgtgagat ggcaaaacat ttgccttcct taaatgaaat
     1681 ggagcttcaa acgctacagt tgatgggaga tgatgtgtct gttatagagc tggatttatc
     1741 ctcacagatc attgaaaaca actccagctt ttccaaaatg attactttaa tgggacaaaa
     1801 atacctgctg ccaccacaaa gcagctttct tttgtctgac atttcttgta tgcagccact
     1861 tcttaactgt ggtaagacat ttgatgcaat tgtgattgat ccaccatggg agaacaaatc
     1921 ggttaaaaga agtaacaggt acagtagttt atcaccacag cagataaagc gaatgcctat
     1981 ccctaaactg gctgctgctg actgtctcat tgttacttgg gtgaccaaca ggcagaaaca
     2041 tctgtgtttt gtgaaggagg aactatatcc ttcgtggtct gtggaagttg ttgcagagtg
     2101 gtactgggta aaaatcacaa attcaggaga gtttgtgttc ccgttggatt ctccacacaa
     2161 aaagccttat gaatgtcttg tactggggag agttaaagaa aaaactccct tagcattaag
     2221 gaatccagat gtaagaatac ccccagttcc agatcagaag ttgattgtca gtgtgccctg
     2281 tgttcttcac tcacacaaac cgcctcttac cgaagttctg agagactaca tcaagccagg
     2341 tggccagtgc ttggaattgt ttgctaggaa tttacagcca ggttggatga gttggggcaa
     2401 cgaagtcctc aagtttcagc acatggatta tttcattgct ctggagtccg gatgctgact
     2461 gtaaaagttg tgctgtcttt acctcagctc cttccttcaa acttatttcc ccttgtacca
     2521 gtcagtgtgc ttagacatat gaacttttac accattcagt gtacccagaa gtataaattt
     2581 tataccaatc aatgtgcata gaaatataca tacatctcct acagcccttt atagtgagtc
     2641 tcttccttcc ttccttgatc tctggcgaac agtggtttgt tttcttacgt tagtgtttcc
     2701 taacgttgtg tctggcttct tccactgagc attcaggaaa accactgaca ctgtatataa
     2761 accactcgct gcttactaag gggattgtta gactgaaagt acttggacaa gcttgcttca
     2821 cctacccact gatttcatca gacattaatt ttgaggtttg ttcatctttt gagggcctaa
     2881 gactaatatc ttttattatt cctttaaact gccagttgaa tacaaggctc attgaattat
     2941 taagtatatt cctttaagct ttttatggag ttttcctgtc taaatttttt gatttgtttc
     3001 catgcaccag atatcctgac ctgcacctga ctttctgcct taaaaataaa accaagggca
     3061 cacctagctt gagactagct tagttgttgc ccttgctctg ccctccatgt gggatttatc
     3121 tactgtgtcc atgtcagttc tcaaactcat cttccctggg gtcccaagtc tcccttttca
     3181 agctcccaac tctagagaca gttcatctag ttggatggac actcacaatg tagcccaggt
     3241 tagccttcaa ctttaatctt cttacattgg gttccaggca tgcatcatta taccaggccc
     3301 tagatgtaaa ccctgaaaga atttttagtt tcaagaacac atgaggttaa ggcttttcta
     3361 ctcatatgat tcaaaagact gtaactattt caaagggcta gtctatgttt tttctttttc
     3421 tttaattttt ttaaaaaagt actatttcta tgtatttggg tgtttgtata tatgtatgtg
     3481 cactatgtat gtgcctgatg cctgcatagg ccaaaagaat cattagatcc cctggaggga
     3541 tgattgtgag ccattcttta gaagagcaac ccgtgctcta aaccgctaaa ccaccttcag
     3601 ccttgggcct cacattcttt gaaggagttt ctatgtcccc tcacttttcc tatcatgtac
     3661 caccaacttg ttgcgggctt ggggtttccc actcgcaccc gtttctgaaa tgactctgag
     3721 acttaattgt ttatgaataa atgcctaggc cataagtttt ggctcatttc ccaactaatt
     3781 tgtaacttat ataatctgtt tgttctagtt tgtgtctgcc acatggttgg ttacctctcc
     3841 ttagtttcat atgcccgatt gctcttagtc caggtggccg atcttactgc tcttgtatct
     3901 ttcccagaat tctttctttc tctgctggat gtcccacctt ctatttcctg cctcagctaa
     3961 taggccatta ggtttttatt gacaggtgat gcttctacac agtacataag agattttctc
     4021 tacaacttgt cacatcctcg gaagtgctaa agcactgtgt gtacagttcc cagtttacca
     4081 atatcttgct ttatcctcaa tacatgctta tgaagataca gctaagatga gggagcaggt
     4141 cctcagaata actggtgtgg ctggagacta agaattgaat tctatttggg ttccttactg
     4201 aaggtacttt atgttgtacc atttgctaat gaacctgaga tgtttattca ttttcatttt
     4261 taaaaatttt atgcttatag tgtcttgttt gcatgtatgt atgtgtgcta catgtgtgcc
     4321 ttgtgtcagt aaatgtcaga agatgatgtt ggatactcta gaactgtagt tacaggtggt
     4381 tgcgagctgc cacacatgtg ctaggaattt gaacagcaag tacttttaac cataaagctg
     4441 tctctttatt cctgaacctg agatcttaac agttgtatta gctcctactc aaagggctca
     4501 ttgtgtatat gtttagaata tttcttacat tatcatggaa acatataccc ttttcagtgt
     4561 acttcagcat cacatagcat ttgtttcctt aagttcgtga cacagtgttt cctgttaaag
     4621 attttatagg taaattgata gcctgagaga ggtttaagat ctaatgggct ggtaacaact
     4681 ttgactaagc cattgggttt atctactcag tcacatttta aggaagttct acaaaacact
     4741 tgagtacagg tagttaaaag ggggagattc cacagtccaa ctcatgttgt ctgttaatct
     4801 ctgtttggct gtatgaccaa cccaaaattc tacagggagt cttaattacc agttcagtta
     4861 ctaatctact tgagtttgat gacaagtcag aactgtatgt tgctgaatca cacagccaac
     4921 cttttctgtg taaatattct taaatatcta gatctcccag ttcctgtgaa cttgagttag
     4981 atctacagat tttcctccca agatcaatcc aaattcttgg accaggtaag aacagatgac
     5041 ttgtaggagt ggagaacaga ggagcaacat tgtccacact ggtctttcag gtttgacagg
     5101 cctagtatcc agtgttgaca gcaggctacc tacagtgaca gctcaaggaa gcaaatggtc
     5161 ggtggcagta tgagaattgc tttattccat gttgttaaag acaatatagc atccagatgt
     5221 tttgtcattt aacacaggct ggttttgaac ttgctatgta attaaggctc gtacttttac
     5281 ccaataaacc tggcctaaaa atcatcttta ctagagaaac atacaaactt atgcaacaca
     5341 atatatagat accagcacca tctcagatat ataatccaaa aggcagagcc ccttgggctc
     5401 catgtgatgg ttatttttga cagcatactt cattgattga aagtacctta agaaagtcac
     5461 aaacgacatc tagtgtgcct gagaatgttc tcagggagta ttcatcaggg aagaaactcc
     5521 tgctgcattt gtgaggctcc atatgatttg ctaggggtcc atgtgaaata acaggagaaa
     5581 gaggaaaaaa aaaaaaacca gctggcatga ccatactttt gctctctggc tgtcatgtaa
     5641 accgcacagc tttgccatgc tctcccttcc ctatcccacc ccactataat ggactaaact
     5701 aaaaactgag caaaactgag ctttccttcc cttaagttgt ttatgtcagg tgtgctgtcc
     5761 cagggatgag gaagctaaaa tggtttcctg gtactttgag cagattgcag cttactctcc
     5821 cagttgttac tcacgactgt gaaagcagca gcaacagaac acaggcgtgc acttctgcca
     5881 tggagaagca gcagcattag gaaatgctgt ttctcctttt aagcatctgt ctcctccatc
     5941 tttcccagca tcggttagca tcgatgtacc tggtactggc tcacttctgg ataggatttg
     6001 catccagagt ctgttaagat ttgcgtttgg tgtacactgc tgcaaaacca ggagaaagct
     6061 gagctcaaca gcagaggggc aactgtcagg ccacagctgg gctgcttttg gagcctttca
     6121 gtgtggctca gataatctct ctccttgttt ataaggttat atgaggtagc ataccaatgc
     6181 atggcagacc aatgtgccaa aatatgcttt ggactgaggt tgattttaag ctgaggttga
     6241 taatcagaaa aaagaaaaaa atacctgcct tgacctcatt tgttagaaag caggatatga
     6301 gtttataaag atggctgtcc tttctctata aggaaaggct gaggactgaa gtgtatcatt
     6361 aacttgaagg aattctatgt ccttatcatc ctggggacaa tacctttcta tcaccagcac
     6421 tggcttctcc acatctcata aggtaagaga ttcagatcct ttccctttgt tttagctctt
     6481 ctctggttta ttattctctg ttgaagatat tgttctaaac cagactgcta agccacttgt
     6541 gggaatttct tattccctgg gtaactcctg tgtgggtttg aaatacacat gtttataagc
     6601 ttctgtttgt ttttctcttg ttaatctgtc ttttatagca gaggccccag caaagaattt
     6661 atacagatag agggaacatt tttccctccc tcatacatac cacattgaaa ctgccaatct
     6721 ataatgactt aatgagcaca ctcttacctt aatatatccg tggacttaaa tgaatagaca
     6781 tgacttgtgt aagaaattag cagactgtca ctgtggagtg gggcaggagc ttagggatca
     6841 ctaagaggga aaatgaaggg gcacattgat taaatttcat tctatttcct aactcaggct
     6901 agtggttgta atgttgttaa gagtcaatag gcaacagaat gtgatatttc agaagcgtta
     6961 caatgacaca ttgtcctcta gattgtaaac ttaaaaaata gatatgaatg tacatgattt
     7021 cgtcaattta ataaaatgat attaaattta ctgatgaatt aaagacttgt ttttctacat
     7081 tacccattta aaataaataa cagtatttaa aacatgcaaa aaaaaaaaaa aaaaaaaaaa
     7141 aaaaaaa
//
