LOCUS       NM_026197               2718 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus methyltransferase 16, N6-methyladenosine (Mettl16),
            mRNA.
ACCESSION   NM_026197
VERSION     NM_026197.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Han,Y., Sun,K., Yu,S., Qin,Y., Zhang,Z., Luo,J., Hu,H., Dai,L.,
            Cui,M., Jiang,C., Liu,F., Huang,Y., Gao,P., Chen,X., Xin,T.,
            Ren,X., Wu,X., Song,J., Wang,Q., Tang,Z., Chen,J., Zhang,H.,
            Zhang,X., Liu,M. and Luo,D.
  TITLE     A Mettl16/m6A/mybl2b/Igf2bp1 axis ensures cell cycle progression of
            embryonic hematopoietic stem and progenitor cells
  JOURNAL   EMBO J 43 (10), 1990-2014 (2024)
   PUBMED   38605226
  REMARK    GeneRIF: A Mettl16/m[6]A/mybl2b/Igf2bp1 axis ensures cell cycle
            progression of embryonic hematopoietic stem and progenitor cells.
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2718)
  AUTHORS   Han,L., Dong,L., Leung,K., Zhao,Z., Li,Y., Gao,L., Chen,Z., Xue,J.,
            Qing,Y., Li,W., Pokharel,S.P., Gao,M., Chen,M., Shen,C., Tan,B.,
            Small,A., Wang,K., Zhang,Z., Qin,X., Yang,L., Wunderlich,M.,
            Zhang,B., Mulloy,J.C., Marcucci,G., Chen,C.W., Wei,M., Su,R.,
            Chen,J. and Deng,X.
  TITLE     METTL16 drives leukemogenesis and leukemia stem cell self-renewal
            by reprogramming BCAA metabolism
  JOURNAL   Cell Stem Cell 30 (1), 52-68 (2023)
   PUBMED   36608679
REFERENCE   4  (bases 1 to 2718)
  AUTHORS   Yoshinaga,M., Han,K., Morgens,D.W., Horii,T., Kobayashi,R.,
            Tsuruyama,T., Hia,F., Yasukura,S., Kajiya,A., Cai,T., Cruz,P.H.C.,
            Vandenbon,A., Suzuki,Y., Kawahara,Y., Hatada,I., Bassik,M.C. and
            Takeuchi,O.
  TITLE     The N6-methyladenosine methyltransferase METTL16 enables
            erythropoiesis through safeguarding genome integrity
  JOURNAL   Nat Commun 13 (1), 6435 (2022)
   PUBMED   36307435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2718)
  AUTHORS   Mendel,M., Delaney,K., Pandey,R.R., Chen,K.M., Wenda,J.M.,
            Vagbo,C.B., Steiner,F.A., Homolka,D. and Pillai,R.S.
  TITLE     Splice site m6A methylation prevents binding of U2AF35 to inhibit
            RNA splicing
  JOURNAL   Cell 184 (12), 3125-3142 (2021)
   PUBMED   33930289
REFERENCE   6  (bases 1 to 2718)
  AUTHORS   Shima,H., Matsumoto,M., Ishigami,Y., Ebina,M., Muto,A., Sato,Y.,
            Kumagai,S., Ochiai,K., Suzuki,T. and Igarashi,K.
  TITLE     S-Adenosylmethionine Synthesis Is Regulated by Selective
            N6-Adenosine Methylation and mRNA Degradation Involving METTL16 and
            YTHDC1
  JOURNAL   Cell Rep 21 (12), 3354-3363 (2017)
   PUBMED   29262316
REFERENCE   7  (bases 1 to 2718)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   8  (bases 1 to 2718)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 2718)
  AUTHORS   Gehman,L.T., Meera,P., Stoilov,P., Shiue,L., O'Brien,J.E.,
            Meisler,M.H., Ares,M. Jr., Otis,T.S. and Black,D.L.
  TITLE     The splicing regulator Rbfox2 is required for both cerebellar
            development and mature motor function
  JOURNAL   Genes Dev 26 (5), 445-460 (2012)
   PUBMED   22357600
REFERENCE   10 (bases 1 to 2718)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL607024.17
            and AL604066.9.
            
            On Apr 29, 2007 this sequence version replaced NM_026197.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK012739.1, AK011780.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-122               AL607024.17        123334-123455
            123-250             AL607024.17        125993-126120
            251-450             AL607024.17        136529-136728
            451-591             AL604066.9         4118-4258
            592-707             AL604066.9         8766-8881
            708-850             AL604066.9         12545-12687
            851-920             AL604066.9         20394-20463
            921-1010            AL604066.9         21816-21905
            1011-1184           AL604066.9         33433-33606
            1185-2718           AL604066.9         33722-35255
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.76 cM"
     gene            1..2718
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="methyltransferase 16, N6-methyladenosine"
                     /db_xref="GeneID:67493"
                     /db_xref="MGI:MGI:1914743"
     exon            1..122
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     CDS             123..1784
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /EC_number="2.1.1.346"
                     /EC_number="2.1.1.348"
                     /note="putative methyltransferase METT10D;
                     methyltransferase-like protein 16; methyltransferase like
                     16; methyltransferase 10 domain-containing protein;
                     methyltransferase 10 domain containing;
                     N6-adenosine-methyltransferase METTL16; U6 small nuclear
                     RNA (adenine-(43)-N(6))-methyltransferase; RNA
                     N6-adenosine-methyltransferase METTL16"
                     /codon_start=1
                     /product="RNA N(6)-adenosine-methyltransferase METTL16"
                     /protein_id="NP_080473.1"
                     /db_xref="CCDS:CCDS25036.1"
                     /db_xref="GeneID:67493"
                     /db_xref="MGI:MGI:1914743"
                     /translation="MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSL
                     NFKDPEAVRALTCTLLREDFGLSIDIPLERLIPTVPLRLNYIHWVEDLIGHQDSDKTT
                     LRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFNYAKKNVEQNNLSDLIKVVKV
                     PQKTLLMDALKEESEIVYDFCMCNPPFFANQLEAKGVNSRNSRRPPPSSVNTGGITEI
                     MAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTFTEFC
                     QGRTMRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLESVMKELSLKASSLGSE
                     TAEGIVVVTTWIEKILTDLKVQHKRIPCGREEVSLFLTAIENSWIHLRRKRRERVRQL
                     REVPRAPEDVILALEERKSTPKELSSGQDVAHSPQESALCGLDVPGGEAAADGGHCLS
                     QKLLCQEETPEATEDERDEERGGMEAMESCKGSSNGAQDGEASEKGDRLDGAAGRYLF
                     KCLVNIKKEAGDAVVEMHWVEGQNRDLMNQLCTYVRNQILRLVAS"
     exon            123..250
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            251..450
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            451..591
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            592..707
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            708..850
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            851..920
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1010
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1184
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..2718
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2143..2148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="hexamer: AATAAA"
     polyA_site      2150
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="major polyA site"
     polyA_site      2165
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="major polyA site"
     regulatory      2601..2606
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="hexamer: AATAAA"
     polyA_site      2623
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="major polyA site"
     regulatory      2694..2699
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="hexamer: AATATA"
     polyA_site      2717
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /note="major polyA site"
ORIGIN      
        1 aagatggcgg cgcgcttgga gcgtagcatc tgcgtttcca ggagcttggc tgtgtgagtg
       61 gtgtgggctt ggtgattgcg ggcctgtccg cactccgtcc gcctgctcca ttggcctgcg
      121 agatggctct aagcaaatca atgcatgcaa gaaatagata caaggacaaa ccacctgact
      181 tcgcatatct ggcatctaaa tatccagatt ttaagcagca tattcagata aatctgaatg
      241 ggagagtaag tcttaatttt aaagaccccg aagcagtcag agctctgact tgtactctcc
      301 tgagagagga ctttggactt tctattgata ttcctttgga gagactaatt cccacagtcc
      361 ccttgagact taactatatt cactgggtag aagatttgat tggtcaccag gattcggaca
      421 aaactactct ccgaagagga attgacatag gtactggggc atcctgcatc tatcccttac
      481 ttggagcaac cttaaatggc tggtatttcc tggcaacaga agtggatgat atgtgtttca
      541 attatgccaa gaaaaatgta gagcagaata acctatctga tctcattaaa gtggtgaaag
      601 tgccgcagaa gacactcctg atggatgcgc ttaaggaaga gtctgagata gtctatgact
      661 tctgtatgtg caaccctccc ttttttgcca accaattgga agccaagggt gtaaattccc
      721 ggaattctcg aagacctcca cctagttctg taaacaccgg gggcatcaca gagattatgg
      781 cagaaggagg tgagctagaa tttgtcaaaa ggatcatcca tgacagttta cagctcaaaa
      841 aaaggttacg gtggtatagc tgtatgctgg gaaagaaatg cagtctggct ccactgaagg
      901 aagagcttag aatccaaggg gttcctaaag tcaccttcac agagttttgt cagggtcgga
      961 caatgagatg ggccttagct tggagttttt atgatgatgt cacagtaccg tcacctccaa
     1021 gtaaacgaag aaaattagaa aagccaagga aacccattac ttttgtagtg ttggagtctg
     1081 tgatgaaaga attgtctctc aaagcttcat ctttgggctc cgagacagcg gaaggcatag
     1141 tggttgtaac aacatggata gaaaagatcc tgactgatct gaaggtgcaa cataagcgaa
     1201 ttccctgtgg aagagaagaa gttagccttt tcctgacagc catagaaaac tcttggattc
     1261 atttaaggcg gaagagaaga gagcgagtga ggcagctgcg ggaggtgcct cgggctcctg
     1321 aggacgtcat cttagccttg gaagagagaa agtccactcc caaagagctg agtagtggcc
     1381 aagacgtggc ccacagcccc caagagagtg ccctgtgtgg gcttgatgtg ccgggcggcg
     1441 aggctgctgc tgatggaggc cattgcctca gccagaagtt actgtgccag gaggagactc
     1501 ccgaggccac ggaggatgaa agggatgagg aaaggggagg gatggaggcc atggaaagtt
     1561 gtaaaggctc cagcaatgga gcccaggacg gagaggcttc tgagaagggg gatcgcctgg
     1621 atggggcggc cggacgctac ctgtttaagt gtttagtcaa cattaagaag gaagctggcg
     1681 atgccgtggt tgaaatgcat tgggttgagg gccagaatag ggatttgatg aaccagcttt
     1741 gcacttacgt acgtaaccaa attttgaggc ttgttgctag ttaattctta atttcttcct
     1801 cagttagaaa agtgatgtat ggggctaggg atatgatgca atgaccttat gtgcttagta
     1861 tccagaagtt accctgtgtt gagtccctta catctaacat cattacaagg aaaaaaaaaa
     1921 cccatattat aaaatagctt gctttggagg tagagcctat tgggaggcca gcagaatttc
     1981 accactggtc catgagtgac accatgtgga atcttcttta aaaaacacaa atttagttgg
     2041 atgtgctctg tggcacttgt aaccccacta cttggaggca aaggcaggaa gatggctatg
     2101 ggtgtaaggg cagcctgtgc tacagagaca gacactcgtc taaataaaaa gaaggaaaaa
     2161 ggaaagcagc agtggatcca tttttaaccc ttgccctctt cacagcagct aaacctagga
     2221 ggagcagatc atcgcagcca aaggccagag gggctctggt ggcatctggt gtgggtcagg
     2281 gctgcctaga aagtgtctgt caccaagggt tgcttggtga caagggcaac agtgttctcc
     2341 agtggaggtc ctgctcatgg ctgccttcaa gatatctgct ttctcaagga aacagaattg
     2401 agcgagctgg ttcccagaaa ggagctagcc tgttgatagg tgacgagagt gtcagggaga
     2461 gtcatcttag tcgagccaag gagagggcct gtctggggaa cacacaggaa gctgcacgtt
     2521 catcgcccac cactattctg acattgccta tttcagaacc agagttgaat aagcaaaact
     2581 ctgagatgta gctgaggcac aataaagtgt acccaccaac ccaccgacag cattgttttg
     2641 taacaattgt actgcctttt tttttaaatg tgaaatggaa tcttttggtt tttaatataa
     2701 gacttaataa ttgaaaac
//
