LOCUS       NP_080473                553 aa            linear   ROD 28-APR-2025
DEFINITION  RNA N(6)-adenosine-methyltransferase METTL16 [Mus musculus].
ACCESSION   NP_080473
VERSION     NP_080473.1
DBSOURCE    REFSEQ: accession NM_026197.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 553)
  AUTHORS   Han,Y., Sun,K., Yu,S., Qin,Y., Zhang,Z., Luo,J., Hu,H., Dai,L.,
            Cui,M., Jiang,C., Liu,F., Huang,Y., Gao,P., Chen,X., Xin,T.,
            Ren,X., Wu,X., Song,J., Wang,Q., Tang,Z., Chen,J., Zhang,H.,
            Zhang,X., Liu,M. and Luo,D.
  TITLE     A Mettl16/m6A/mybl2b/Igf2bp1 axis ensures cell cycle progression of
            embryonic hematopoietic stem and progenitor cells
  JOURNAL   EMBO J 43 (10), 1990-2014 (2024)
   PUBMED   38605226
  REMARK    GeneRIF: A Mettl16/m[6]A/mybl2b/Igf2bp1 axis ensures cell cycle
            progression of embryonic hematopoietic stem and progenitor cells.
REFERENCE   2  (residues 1 to 553)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 553)
  AUTHORS   Han,L., Dong,L., Leung,K., Zhao,Z., Li,Y., Gao,L., Chen,Z., Xue,J.,
            Qing,Y., Li,W., Pokharel,S.P., Gao,M., Chen,M., Shen,C., Tan,B.,
            Small,A., Wang,K., Zhang,Z., Qin,X., Yang,L., Wunderlich,M.,
            Zhang,B., Mulloy,J.C., Marcucci,G., Chen,C.W., Wei,M., Su,R.,
            Chen,J. and Deng,X.
  TITLE     METTL16 drives leukemogenesis and leukemia stem cell self-renewal
            by reprogramming BCAA metabolism
  JOURNAL   Cell Stem Cell 30 (1), 52-68 (2023)
   PUBMED   36608679
REFERENCE   4  (residues 1 to 553)
  AUTHORS   Yoshinaga,M., Han,K., Morgens,D.W., Horii,T., Kobayashi,R.,
            Tsuruyama,T., Hia,F., Yasukura,S., Kajiya,A., Cai,T., Cruz,P.H.C.,
            Vandenbon,A., Suzuki,Y., Kawahara,Y., Hatada,I., Bassik,M.C. and
            Takeuchi,O.
  TITLE     The N6-methyladenosine methyltransferase METTL16 enables
            erythropoiesis through safeguarding genome integrity
  JOURNAL   Nat Commun 13 (1), 6435 (2022)
   PUBMED   36307435
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 553)
  AUTHORS   Mendel,M., Delaney,K., Pandey,R.R., Chen,K.M., Wenda,J.M.,
            Vagbo,C.B., Steiner,F.A., Homolka,D. and Pillai,R.S.
  TITLE     Splice site m6A methylation prevents binding of U2AF35 to inhibit
            RNA splicing
  JOURNAL   Cell 184 (12), 3125-3142 (2021)
   PUBMED   33930289
REFERENCE   6  (residues 1 to 553)
  AUTHORS   Shima,H., Matsumoto,M., Ishigami,Y., Ebina,M., Muto,A., Sato,Y.,
            Kumagai,S., Ochiai,K., Suzuki,T. and Igarashi,K.
  TITLE     S-Adenosylmethionine Synthesis Is Regulated by Selective
            N6-Adenosine Methylation and mRNA Degradation Involving METTL16 and
            YTHDC1
  JOURNAL   Cell Rep 21 (12), 3354-3363 (2017)
   PUBMED   29262316
REFERENCE   7  (residues 1 to 553)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   8  (residues 1 to 553)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (residues 1 to 553)
  AUTHORS   Gehman,L.T., Meera,P., Stoilov,P., Shiue,L., O'Brien,J.E.,
            Meisler,M.H., Ares,M. Jr., Otis,T.S. and Black,D.L.
  TITLE     The splicing regulator Rbfox2 is required for both cerebellar
            development and mature motor function
  JOURNAL   Genes Dev 26 (5), 445-460 (2012)
   PUBMED   22357600
REFERENCE   10 (residues 1 to 553)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL607024.17
            and AL604066.9.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK012739.1, AK011780.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.76 cM"
     Protein         1..553
                     /product="RNA N(6)-adenosine-methyltransferase METTL16"
                     /EC_number="2.1.1.346"
                     /EC_number="2.1.1.348"
                     /note="putative methyltransferase METT10D;
                     methyltransferase-like protein 16; methyltransferase like
                     16; methyltransferase 10 domain-containing protein;
                     methyltransferase 10 domain containing;
                     N6-adenosine-methyltransferase METTL16; U6 small nuclear
                     RNA (adenine-(43)-N(6))-methyltransferase; RNA
                     N6-adenosine-methyltransferase METTL16"
                     /calculated_mol_wt=62210
     Region          1..291
                     /region_name="Methyltransf_10"
                     /note="RNA methyltransferase; pfam05971"
                     /db_xref="CDD:399160"
     Region          17..20
                     /region_name="RNA-binding.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Site            order(109..115,133..134,160..162,184)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     Region          163..167
                     /region_name="K-loop.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          199..211
                     /region_name="RNA-binding.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          250..254
                     /region_name="RNA-binding.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          277..283
                     /region_name="RNA-binding.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          289..400
                     /region_name="VCR 1.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Site            329
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q86W50; propagated from
                     UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Site            425
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Site            429
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          457..496
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Site            463
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q9CQG2.1)"
     Region          506..553
                     /region_name="VCR 2.
                     /evidence=ECO:0000250|UniProtKB:Q86W50"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9CQG2.1)"
     CDS             1..553
                     /gene="Mettl16"
                     /gene_synonym="2610100D03Rik; 2810013M15Rik;
                     A830095F14Rik; Mett10d"
                     /coded_by="NM_026197.3:123..1784"
                     /db_xref="CCDS:CCDS25036.1"
                     /db_xref="GeneID:67493"
                     /db_xref="MGI:MGI:1914743"
ORIGIN      
        1 malsksmhar nrykdkppdf aylaskypdf kqhiqinlng rvslnfkdpe avraltctll
       61 redfglsidi plerliptvp lrlnyihwve dlighqdsdk ttlrrgidig tgasciypll
      121 gatlngwyfl atevddmcfn yakknveqnn lsdlikvvkv pqktllmdal keeseivydf
      181 cmcnppffan qleakgvnsr nsrrpppssv ntggiteima eggelefvkr iihdslqlkk
      241 rlrwyscmlg kkcslaplke elriqgvpkv tftefcqgrt mrwalawsfy ddvtvpspps
      301 krrklekprk pitfvvlesv mkelslkass lgsetaegiv vvttwiekil tdlkvqhkri
      361 pcgreevslf ltaienswih lrrkrrervr qlrevprape dvilaleerk stpkelssgq
      421 dvahspqesa lcgldvpgge aaadgghcls qkllcqeetp eatederdee rggmeamesc
      481 kgssngaqdg easekgdrld gaagrylfkc lvnikkeagd avvemhwveg qnrdlmnqlc
      541 tyvrnqilrl vas
//
