LOCUS       NM_008590               2579 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus mesoderm specific transcript (Mest), transcript
            variant 2, mRNA.
ACCESSION   NM_008590 XM_974235 XM_974274
VERSION     NM_008590.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Zhou,B., Feng,C., Sun,S., Chen,X., Zhuansun,D., Wang,D., Yu,X.,
            Meng,X., Xiao,J., Wu,L., Wang,J., Wang,J., Chen,K., Li,Z., You,J.,
            Mao,H., Yang,S., Zhang,J., Jiao,C., Li,Z., Yu,D., Wu,X., Zhu,T.,
            Yang,J., Xiang,L., Liu,J., Chai,T., Shen,J., Mao,C.X., Hu,J.,
            Hao,X., Xiong,B., Zheng,S., Liu,Z. and Feng,J.
  TITLE     Identification of signaling pathways that specify a subset of
            migrating enteric neural crest cells at the wavefront in mouse
            embryos
  JOURNAL   Dev Cell 59 (13), 1689-1706 (2024)
   PUBMED   38636517
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Feng,W., Bais,A., He,H., Rios,C., Jiang,S., Xu,J., Chang,C.,
            Kostka,D. and Li,G.
  TITLE     Single-cell transcriptomic analysis identifies murine heart
            molecular features at embryonic and neonatal stages
  JOURNAL   Nat Commun 13 (1), 7960 (2022)
   PUBMED   36575170
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2579)
  AUTHORS   Zimmerman,S.M., Fropf,R., Kulasekara,B.R., Griswold,M., Appelbe,O.,
            Bahrami,A., Boykin,R., Buhr,D.L., Fuhrman,K., Hoang,M.L., Huynh,Q.,
            Isgur,L., Klock,A., Kutchma,A., Lasley,A.E., Liang,Y.,
            McKay-Fleisch,J., Nelson,J.S., Nguyen,K., Piazza,E., Rininger,A.,
            Zollinger,D.R., Rhodes,M. and Beechem,J.M.
  TITLE     Spatially resolved whole transcriptome profiling in human and mouse
            tissue using Digital Spatial Profiling
  JOURNAL   Genome Res 32 (10), 1892-1905 (2022)
   PUBMED   36100434
REFERENCE   4  (bases 1 to 2579)
  AUTHORS   Anunciado-Koza,R.P., Stohn,J.P., Hernandez,A. and Koza,R.A.
  TITLE     Social and maternal behavior in mesoderm specific transcript
            (Mest)-deficient mice
  JOURNAL   PLoS One 17 (7), e0271913 (2022)
   PUBMED   35867696
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2579)
  AUTHORS   Lee,D.R., Rhodes,C., Mitra,A., Zhang,Y., Maric,D., Dale,R.K. and
            Petros,T.J.
  TITLE     Transcriptional heterogeneity of ventricular zone cells in the
            ganglionic eminences of the mouse forebrain
  JOURNAL   Elife 11, e71864 (2022)
   PUBMED   35175194
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2579)
  AUTHORS   Lefebvre,L., Viville,S., Barton,S.C., Ishino,F. and Surani,M.A.
  TITLE     Genomic structure and parent-of-origin-specific methylation of Peg1
  JOURNAL   Hum Mol Genet 6 (11), 1907-1915 (1997)
   PUBMED   9302270
REFERENCE   7  (bases 1 to 2579)
  AUTHORS   Kobayashi,S., Kohda,T., Miyoshi,N., Kuroiwa,Y., Aisaka,K.,
            Tsutsumi,O., Kaneko-Ishino,T. and Ishino,F.
  TITLE     Human PEG1/MEST, an imprinted gene on chromosome 7
  JOURNAL   Hum Mol Genet 6 (5), 781-786 (1997)
   PUBMED   9158153
REFERENCE   8  (bases 1 to 2579)
  AUTHORS   Wada,J., Kumar,A., Ota,K., Wallner,E.I., Batlle,D.C. and
            Kanwar,Y.S.
  TITLE     Representational difference analysis of cDNA of genes expressed in
            embryonic kidney
  JOURNAL   Kidney Int 51 (5), 1629-1638 (1997)
   PUBMED   9150484
REFERENCE   9  (bases 1 to 2579)
  AUTHORS   Nishita,Y., Yoshida,I., Sado,T. and Takagi,N.
  TITLE     Genomic imprinting and chromosomal localization of the human MEST
            gene
  JOURNAL   Genomics 36 (3), 539-542 (1996)
   PUBMED   8884280
REFERENCE   10 (bases 1 to 2579)
  AUTHORS   Kaneko-Ishino,T., Kuroiwa,Y., Miyoshi,N., Kohda,T., Suzuki,R.,
            Yokoyama,M., Viville,S., Barton,S.C., Ishino,F. and Surani,M.A.
  TITLE     Peg1/Mest imprinted gene on chromosome 6 identified by cDNA
            subtraction hybridization
  JOURNAL   Nat Genet 11 (1), 52-59 (1995)
   PUBMED   7550314
  REMARK    GeneRIF: Mest/Peg1 gene is imprinted, with preferential expression
            from the paternal allele.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ162886.1, AK032881.1 and BF608470.1.
            
            On Nov 9, 2011 this sequence version replaced NM_008590.1.
            
            Transcript Variant: This variant (2) lacks an alternate exon in the
            5' coding region, compared to variant 1. The resulting protein
            (isoform 2) is shorter when it is compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006639.1, AK168743.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 7550314
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 CJ162886.1         2-4
            4-2493              AK032881.1         3-2492
            2494-2579           BF608470.1         480-565
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.53 cM"
     gene            1..2579
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /note="mesoderm specific transcript"
                     /db_xref="GeneID:17294"
                     /db_xref="MGI:MGI:96968"
     exon            1..286
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             261..1268
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     mesoderm-specific transcript protein; paternally-expressed
                     gene 1 protein"
                     /codon_start=1
                     /product="mesoderm-specific transcript protein isoform 2"
                     /protein_id="NP_032616.1"
                     /db_xref="CCDS:CCDS19979.1"
                     /db_xref="GeneID:17294"
                     /db_xref="MGI:MGI:96968"
                     /translation="MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWK
                     TSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIAL
                     DFLGFGFSDKPRPHQYSIFEQASIVESLLRHLGLQNRRINLLSHDYGDIVAQELLYRY
                     KQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLT
                     PVFGPYTRPTESELWDMWAVIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVSIPI
                     HFIYGPLDPINPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINS
                     F"
     exon            287..441
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            442..521
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            522..599
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            600..736
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            737..795
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            796..836
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            837..907
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1009
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1086
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1150
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..2565
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2544..2549
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /note="hexamer: AACAAA"
     polyA_site      2561
                     /gene="Mest"
                     /gene_synonym="Peg1"
                     /note="major polyA site"
ORIGIN      
        1 tctgcttcgg ccaccagcac atcccggtgc ttcttctcag gcgcagcagc tttcctctgc
       61 ggcagccgca cctcgccaaa cggcgtagtg ctgcaggctc gcccgagttg ctgcttgctg
      121 cctctgctgc cgctgccgcg ggccgccctg cgcggaccgt aggctgcgca gacgccacct
      181 ccgatcctgt atcgctgcgg gcgcctcggc gcgccctgtg atccgcaatc ctgcggcggg
      241 cggcatggga taatgcggcc atggtgcgcc gagatcgctt gcgcaggatg agagagtggt
      301 gggtccaagt agggctcctg gctgtgccct tgctggctgc gtacctgcac atcccgcccc
      361 ctcagctctc ccctgctctg cactcatgga agacttctgg caagtttttc acctacaaag
      421 gcctacgcat cttctaccaa gattctgtcg gtgtggtcgg aagccctgag atagttgtgc
      481 ttttacatgg ctttccaaca tccagctatg actggtacaa gatctgggaa gggctgaccc
      541 tgaggttcca tcgagtgatc gcccttgatt tcttaggctt tggcttcagt gacaagccga
      601 gaccacatca gtactccata tttgagcagg ccagcatcgt agagtccctt ctgcggcacc
      661 tggggctcca gaaccgcaga atcaacctgc tgtctcacga ttatggagat atcgttgctc
      721 aggaactgct gtacaggtac aagcagaatc gatctggccg gctcaccata aagagtctct
      781 gtctgtcgaa tggaggtatc tttcctgaga cgcatcgtcc tctccttctc caaaagctcc
      841 tcaaagacgg aggtgtgctt tcccccatcc tcaccaggct catgaatttc ttcgtgttct
      901 ctcgaggtct cactccagtc tttggaccgt atactcgacc cactgagagt gagctgtggg
      961 acatgtgggc tgtgattcgc aacaatgacg gcaacctggt catcgacagt cttttacagt
     1021 acattaatca aaggaagaaa ttcagaagac gctgggtggg agcgcttgct tctgtgtcca
     1081 tccccattca ttttatctat gggccattgg atcctataaa tccgtatcca gagtttttgg
     1141 agctgtacag gaaaacgctg ccgcggtcca cagtgtcgat tctggatgac cacattagcc
     1201 actacccaca gctagaggat cccatgggct tcttgaatgc atatatgggc ttcatcaact
     1261 ccttctgagc tggaaagagt agcctccctg tattacctcc cccattctcg tatctgtgtg
     1321 tattccactt agaaatgccc aaaagaggtc ctggccatca aatactatcc tttcatcaaa
     1381 gtccatttcc ttcacattgg tgaacaaact acaggaatca ggcagctgga actctatagg
     1441 gtgacacaat agtccacctc atctctaagg gtgacatgat aatcctctca tttctgtgac
     1501 atctgaccaa ataaatagag ctgctgttgt tttgtgttat taggacattc tgactaataa
     1561 cacttttcat ttatgtagaa aaacagcatt agatattctt ttttggtgtt aaatacctca
     1621 aaaaaccccc aaaacataat aacaacaaaa acccagtttc tgtggacttt attaaaatat
     1681 ttagaagtgc tcccttccgg cccaatccta actggaatac agagtgaggg aggagcttgc
     1741 tatctccctt tcaggatctg ttacagttta tgagagggat catcttgtca ctcctttaca
     1801 tttagtttca tcggctgctt ctaacagaca tttttgttaa aacagacatt ggcttagggg
     1861 gctggggaag tagctcagtg gtagtgtgcc tgcctagcat gttaaagccc ttgggttcag
     1921 ttcccagcac tctccccacc ccagcccatc gaacctaaat agacattggc gtaaatacag
     1981 tgttctgagt atgagtctaa gactgatttg catactcatg tatatattta caaacagttg
     2041 gcccttgcta agaagaaaac ctcattatat atagaatttg tgaataaaca ggttgaaatt
     2101 atttgcagaa tttcctaaaa ctgccaggca ttaagaactg catctgtgcc agagatttac
     2161 tgttactgtt tagaaagtcc aagctgaata gtaaataaca gcctaggggc agctcctcca
     2221 gggctgacgt ggaggggaag ggacatgtaa gtgcggaatg aatgtccatt ggctcctcta
     2281 tgatggccgc tcagcatccc gctggctgtt cagaaaggct ttctcatata actcctgtac
     2341 ttaaacaggc aagtttccag agacattaag aacaaattct gaaagaccta agagcaaatg
     2401 gtgctgaata cttttttaag ccacagtttt aacttagtta aagcagcatt attgattatt
     2461 ttaaattcgt tttttaaatt agaaatttca agtatagcaa ctttgaaact ggaatgaatg
     2521 agtgtttatt ttctattaat aaaaacaaac tgtgacaaaa atggaaaaaa aaaaaaaaa
//
